scholarly journals Biodegradation of Pyrethroids by a Hydrolyzing Carboxylesterase EstA from Bacillus cereus BCC01

2019 ◽  
Vol 9 (3) ◽  
pp. 477 ◽  
Author(s):  
Wei Hu ◽  
Qiqi Lu ◽  
Guohua Zhong ◽  
Meiying Hu ◽  
Xin Yi
Keyword(s):  
Bacillus Cereus ◽  
Genomic Library ◽  
Cost Effective ◽  
Gas Chromatography Mass Spectrometry ◽  
Effective Technique ◽  
Ph Values ◽  
Wide Range ◽  
Cost Effective Technique ◽  
Culture Suspension ◽  
Bacterium Bacillus

Microbial degradation has been considered as a rapid, green, and cost-effective technique to reduce insecticide pollutions in a contaminated environment. However, the instability and low efficacy of non-indigenous microorganisms hampers their further exploitation when being introduced into a real environmental matrix. In order to overcome the restriction that these functional microorganisms are under, we investigated the optimal conditions to improve the pyrethroid-degrading ability of one previously isolated bacterium Bacillus cereus BCC01, where 9.6% of the culture suspension (with cell density adjusted to OD600 = 0.6) was inoculated into 50 mL media and cultivated at pH 8 and 30 °C, and its metabolic pathway was illuminated by analyzing the main metabolites via gas chromatography mass spectrometry (GC-MS). Most importantly, a key pyrethroid-hydrolyzing carboxylesterase gene estA was identified from the genomic library of strain BCC01, and then expressed in Escherichia coli BL21 (DE3). After purification, the recombinant protein EstA remained soluble, displaying high degrading activity against different pyrethroids and favorable stability over a wide range of temperatures (from 15 °C to 50 °C) and pH values (6.5–9). Therefore, the EstA-associated biodegradation of pyrethroids was determined, which could provide novel insights to facilitate the practical application of B. cereus BCC01 in the microbial detoxification of pyrethroid contamination.

Reflectance Transformation Imaging for Roman Coin Identification: Archaeology and Education

2016 ◽  
Author(s):  
Ana Crisan
Keyword(s):  
Cost Effective ◽  
School Level ◽  
Effective Technique ◽  
Imperial Period ◽  
Wide Range ◽  
Secondary School Level ◽  
Cost Effective Technique ◽  
Byzantine Period ◽  
Reflectance Transformation Imaging ◽  
Primary And Secondary School

In 2001 the Department of Classics acquired pieces from the Diniacopolous family collection, along with a large number of coins. The majority of these coins were minted in Alexandria and vary in dates from the Hellenistic to the Byzantine period with the bulk from the Roman Imperial Period date range. While some of the coins are in decent condition and their legends and reliefs can be read with the naked eye, most require the use of imaging technology in order to be identified. This presentation will discuss results of a project currently underway to image the coins using Reflectance Transformation Imaging (RTI), a cost effective technique, which has also been used by the department at Cataraqui Cemetery to recover eroded tombstone inscriptions. While some coins were extensively eroded and thus could not be classified, the technique showed impressive results allowing most coins to be identified and dated. The presentation will also outline how RTI can be used in education, as bags of coins can be cheaply acquired by educators, thus allowing students at the primary and secondary school level to actively participate in deciphering corroded coins. This project demonstrates that RTI can be applied to a wide range of artefacts and is a valuable tool in preserving cultural heritage.

Bucket handle meniscus tears in low-resource settings can be successfully treated with a cost-effective technique

2021 ◽  
Author(s):  
Allicia Ostoposides Imada ◽  
James J. O’Hara ◽  
Ignacio L. Proumen ◽  
Pablo S. Molinari ◽  
Daniel C. Wascher ◽  
...  
Keyword(s):  
Cost Effective ◽  
Effective Technique ◽  
Low Resource Settings ◽  
Low Resource ◽  
Meniscus Tears ◽  
Cost Effective Technique ◽  
Bucket Handle

scholarly journals Simple, cost-effective technique for portable digital eletrocorticography

2000 ◽  
Vol 58 (2B) ◽  
pp. 424-427 ◽  
Author(s):  
PAULO R. M. DE BITTENCOURT ◽  
MARCOS C. SANDMANN ◽  
MARLUS S. MORO ◽  
JOÃO C. DE ARAÚJO
Keyword(s):  
Real Time ◽  
Epilepsy Surgery ◽  
Vascular Malformations ◽  
Cost Effective ◽  
New Method ◽  
Cortical Lesions ◽  
Effective Technique ◽  
Seizure Disorders ◽  
Operational Costs ◽  
Cost Effective Technique

We revised 16 patients submitted to epilepsy surgery using a new method of digital, real-time, portable electrocorticography. Patients were operated upon over a period of 28 months. There were no complications. The exam was useful in 13 cases. The low installation and operational costs, the reliability and simplicity of the method, indicate it may be useful for defining the epileptogenic regions in a variety of circumnstances, including surgery for tumors, vascular malformations, and other cortical lesions associated with seizure disorders.

Formation of Periodical Sub-Micron Sized Structures on Silicon by Interfered Nanosecond Laser Pulses

2010 ◽  
Vol 97-101 ◽  
pp. 3803-3806
Author(s):  
Yong Xiang Hu ◽  
Heng Zhang ◽  
Zheng Qiang Yao
Keyword(s):  
Cost Effective ◽  
Laser Pulses ◽  
Laser Beams ◽  
Nanosecond Laser ◽  
Effective Technique ◽  
First Order ◽  
Direct Fabrication ◽  
Cost Effective Technique ◽  
Nanosecond Laser Pulses ◽  
Micro Structuring

Laser interference micro-structuring is a relatively efficient and cost-effective technique for fabricating periodical micro-nano-structuring surfaces. The direct fabrication of sub-micron sized dot array on silicon was performed by four interfering nanosecond laser beams with a diffractive beam splitter. The mechanism to form the dot array was analyzed and it was found that the obtained conical dot array had a negative shape of the interference pattern of four laser beams. A second-order peak between two first-order peaks also occurred due to the liquid-solid expansion.

Telemedicine in the control of intra-ocular pressure

2000 ◽  
Vol 6 (1_suppl) ◽  
pp. 126-128 ◽  
Author(s):  
G Michelson ◽  
W Striebel ◽  
W Prihoda ◽  
Volker Schmidt
Keyword(s):  
Cost Effective ◽  
Western World ◽  
Pressure Curve ◽  
Effective Technique ◽  
Intra Ocular Pressure ◽  
Continuous Evaluation ◽  
Common Causes ◽  
Cost Effective Technique ◽  
Email Message ◽  
Ocular Pressure

Glaucoma is one of the most common causes of blindness in the Western world and a major risk factor is increased intra-ocular pressure. We therefore used telemedicine in its control. Patients measured their intra-ocular pressure several times a day with a portable instrument and the values were then entered into a portable digital assistant. These data were transmitted by a modem to a central server. If the intra-ocular pressure was pathologically high, an email message was automatically sent to the ophthalmologist. The pressure curve, including a statistical analysis, was displayed in an easily readable chart format. Ten patients with glaucoma participated in a trial. Self-tonometry with telemedicine enabled continuous evaluation of the patient by the ophthalmologist. This approach offered the advantage of controlling the treatment remotely. Advantages for the patient were that the measurements were easily done at home under normal conditions, and the patient could control when the measurement and data transmission would be performed. Telemedicine is a cost-effective technique enabling the early diagnosis of pathologically increased intra-ocular pressure.

scholarly journals A cost-effective technique for pure laparoscopic live donor nephrectomy

2006 ◽  
Vol 32 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Tiberio M Siqueira Jr ◽  
Anuar I. Mitre ◽  
Fabiano A. Simoes ◽  
Andre F. Maciel ◽  
Alvaro M. Ferraz ◽  
...  
Keyword(s):  
Cost Effective ◽  
Donor Nephrectomy ◽  
Live Donor ◽  
Effective Technique ◽  
Live Donor Nephrectomy ◽  
Cost Effective Technique ◽  
Laparoscopic Live Donor Nephrectomy

scholarly journals Effect of Salicylic Acid in the Yield of Ricinine in Ricinus communis under Greenhouse Condition

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1902
Author(s):  
Carlos Eduardo Zavala-Gómez ◽  
Eloy Rodríguez-deLeón ◽  
Mamadou Moustapha Bah ◽  
Ana Angélica Feregrino-Pérez ◽  
Juan Campos-Guillén ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Plant Height ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Cost Effective ◽  
Control Group ◽  
Effective Technique ◽  
Greenhouse Condition ◽  
Cost Effective Technique

Castor bean (Ricinus communis) seeds contain ricinine, an alkaloid with insecticidal and insectistatic activities. Elicitation with salicylic acid (SA) has proven to stress R. communis and might modify the ricinine concentration. The aim of this study was to evaluate the concentration of ricinine in the bagasse of seeds from R. communis elicited with exogenous SA under greenhouse conditions. Plants were grown and divided into five groups, which were sprayed with SA and drench with 50 mL 60 days after sowing with concentrations of SA (0, 100, 300, 600 and 900 µM). Clusters were mixed and separated according to the treatment, and dried. The seeds were ground, the oil was extracted by Soxhlet with hexane, and then the bagasse was extracted with methanol. Ricinine was determined by HPLC. Elicitation did not change the plant height or diameter; the control group had 9.17 µg mL−1 of ricinine; and the concentrations followed a hormesis curve with the peak at 300 µM of SA that had a ricinine concentration of 18.25 µg mL−1. Elicitation with SA might be a cost-effective technique to increase ricinine from R. communis bagasse.

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