Riboflavin and Its Effect on Dentin Bond Strength: Considerations for Clinical Applicability—An In Vitro Study

Bioactive collagen crosslinkers propose to render the dentin hybrid layer less perceptive to hydrolytic challenge. This study aims to evaluate whether bond strength of dental resin composite to dentin benefits from riboflavin (RB)-sensitized crosslinking when used in a clinically applicable protocol. A total of 300 human dentin specimens were prepared consistent with the requirements for a macro-shear bond test. RB was applied on dentin, either incorporated in the primer (RBp) of a two-step self-etch adhesive or as an aqueous solution (RBs) before applying the adhesive, and blue light from a commercial polymerization device was used for RB photoactivation. Bonding protocol executed according to the manufacturer’s information served as control. Groups (n = 20) were tested after 1 week, 1 month, 3 months, 6 months or 1 year immersion times (37 °C, distilled water). The different application methods of RB significantly influenced bond strength (p < 0.001) with a medium impact (η2p = 0.119). After 1 year immersion, post hoc analysis identified a significant advantage for RB groups compared to RBp (p = 0.018), which is attributed to a pH-/solvent-dependent efficiency of RB-sensitized crosslinking, stressing the importance of formulation adjustments. We developed an application protocol for RB-sensitized crosslinking with emphasis on clinical applicability to test its performance against a gold-standard adhesive, and are confident that, with a few adjustments to the application solution, RB-sensitized crosslinking can improve the longevity of adhesive restorations in clinics.

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COMPARATIVE EVALUATION OF THE SHEAR BOND STRENGTH OF THREE SELF-ETCH DENTIN BONDING AGENTS TO DENTIN: AN IN-VITRO STUDY

International Journal of Advanced Research ◽

10.21474/ijar01/11872 ◽

2020 ◽

Vol 8 (10) ◽

pp. 454-459

Author(s):

Bhalla V. ◽

◽

K. Goud M. ◽

Chockattu S. ◽

Khera A ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Testing Machine ◽

Group Analysis ◽

In Vitro Study ◽

Bonding Agents ◽

Mandibular Molar ◽

Self Etch

Background:Dentin bonding is an ever-evolving field in adhesive dentistry. With the introduction of newer systems into the market, there is a crucial need to test their efficiency in terms of bond strength. Dual-cured adhesives in theory may provide for a better degree of conversion as compared to conventional light-cured adhesives .Thus, the aim of this study was to compare the shear bond strength of three different self-etch adhesives namely ClearfilSE bond (Kuraray), Tetric N Bond Universal (IvoclarVivadent) and Futura Bond DC (Voco) to dentin. Materials & Methods: Ninety extracted non-carious, intact human mandibular molar teeth were selected for this study. Each tooth was decoronated using a double-sided diamond disc with water coolant to a depth of 2mm from the cusp tip .The cut dentin surface was then abraded against 600-grit wet silicon carbide papers for 60 seconds to produce a uniform smear layer. The root portion of each tooth was mounted on a plastic ring using cold cure acrylic resin. Specimens were then divided into three adhesive groups of 30 teeth each, Group A: ClearfilSE Bond (Kuraray), Group B: Tetric N Bond Universal (IvoclarVivadent), Group C :Futura Bond DC (Voco). All bonding agents were used according to the manufacturers’ instructions, in combination with the resin composite Tetric N Ceram (IvoclarVivadent). The samples were thermocycled, followed by shear bond strength testing using a Universal testing machine (Hounsfield). Data were subjected to statistical analysis using one-way analysis of variance (ANOVA) (P<0.05) and Post hoc Tukey’s test for inter- and intra- group analysis respectively. Results: Clearfil SE Bond yielded the highest shear bond strength values (30.9 ±4.66 MPa) which were statistically significant, followed byTetric N Bond Universal group (29.8 ±4.34) and the lowest shear bond strength values were recorded for Futura Bond DC (18.2 ±3.13). Conclusion: Clearfil SE bond and Tetric N bond Universal can be considered as better options than Futura Bond DC.

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Comparative evaluation of shear bond strength of composite to dentin in presence of different dental liners: An in-vitro study

10.21203/rs.3.rs-909993/v3 ◽

2021 ◽

Author(s):

Ladan Ranjbar Omrani ◽

Saba Tohidkhah ◽

Elham Ahmadi ◽

Mahdi Abbasi ◽

Reza Morvaridi Farimani

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Testing Machine ◽

Glass Ionomer Cement ◽

In Vitro Study ◽

Control Group ◽

The One ◽

Post Hoc

Abstract Background: The aim of the current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

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Comparative Evaluation of Shear Bond Strength of Composite to Dentin in Presence of Calcium Releasing Liners: An In-Vitro Study

10.21203/rs.3.rs-909993/v1 ◽

2021 ◽

Author(s):

Saba Tohidkhah ◽

Elham Ahmadi ◽

Mahdi Abbasi ◽

Reza Morvaridi Farimani ◽

Ladan Ranjbar Omrani

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Testing Machine ◽

Glass Ionomer Cement ◽

In Vitro Study ◽

Control Group ◽

The One ◽

Post Hoc

Abstract Background: The aim of current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

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Comparative evaluation of shear bond strength of composite to dentin in presence of different dantal liners: An in-vitro study

10.21203/rs.3.rs-909993/v2 ◽

2021 ◽

Author(s):

Ladan Ranjbar Omrani ◽

Saba Tohidkhah ◽

Elham Ahmadi ◽

Mahdi Abbasi ◽

Reza Morvaridi Farimani

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Testing Machine ◽

Glass Ionomer Cement ◽

In Vitro Study ◽

Control Group ◽

The One ◽

Post Hoc

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Chlorhexidine Preserves Dentin Bond in vitro

Journal of Dental Research ◽

10.1177/154405910708600115 ◽

2007 ◽

Vol 86 (1) ◽

pp. 90-94 ◽

Cited By ~ 235

Author(s):

M.R.O. Carrilho ◽

R.M. Carvalho ◽

M.F. de Goes ◽

V. di Hipólito ◽

S. Geraldeli ◽

...

Keyword(s):

Bond Strength ◽

Protease Inhibitors ◽

Resin Composite ◽

Artificial Saliva ◽

In Vitro Study ◽

Hybrid Layer ◽

Adhesive Resin ◽

Bond Stability ◽

Etch And Rinse

Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted third molars were sectioned into two halves. One half was customarily restored (etch-and-rinse adhesive/resin composite), and the other was treated with 2% chlorhexidine after being acid-etched before restoration. Specimens were stored in artificial saliva with/without protease inhibitors. Microtensile bond strengths and failure mode distribution under SEM were analyzed immediately after specimens’ preparation and 6 months later. With chlorhexidine, significantly better preservation of bond strength was observed after 6 months; protease inhibitors in the storage medium had no effect. Failure analysis showed significantly less failure in the hybrid layer with chlorhexidine, compared with controls after 6 months. In conclusion, this in vitro study suggests that chlorhexidine might be useful for the preservation of dentin bond strength.

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Micro Push-Out Bond Strength of Resin Composite to Dentin in Primary Dentition Using Three Universal Adhesives with Different pH: An In Vitro Study

Applied Sciences ◽

10.3390/app11146349 ◽

2021 ◽

Vol 11 (14) ◽

pp. 6349

Author(s):

Regina María Del Río Carrillo ◽

Pedro Molinero-Mourelle ◽

Vicente Vera ◽

Pablo Romero Villaba ◽

Elizabeth Casañas ◽

...

Keyword(s):

Bond Strength ◽

Resin Composite ◽

In Vitro Study ◽

Primary Dentition ◽

Vitro Study ◽

Deciduous Tooth ◽

Universal Adhesives ◽

Type Of Failure ◽

Push Out

To evaluate the bond strength of different universal adhesives on deciduous tooth dentineand their relationship with the composition and potential of hydrogen (pH). Methods: An in vitromicro push-out test on 150 samples (n = 50) per group per adhesive, namely, Adhese Universal(ADH; Ivoclar Vivadent), Futurabond U (FUT; Voco GmbH) (Test), and Scotchbond Universal withpre-conditioning (SCO; 3M) (Control), to record bond strength (BS) and type of adhesive failure.Results: The results of the different adhesives (megapascals (MPa)) varied, showing no statisticalsignificance. The corresponding averages are in MPa: ADH, 13.66 2.81; FUT, 14.48 2.88; SCO,14.98 3.96. Additionally, the frequency of type of failure was as follows: mixed (60.7%), adhesive(27.3%), and cohesive (12%). Conclusions: SCO, with a pH of 2.7, showed greater resistance tofracture, while FUT, with a pH of 2.3 and no pre-conditioning, approached the same values, being aone-step adhesive. No relationship was found between failure and type of adhesive.

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The Influence of Different Surface Cleansing Agents on Shear Bond Strength of Contaminated Lithium Disilicate Ceramic: An In Vitro Study

International Journal of Dentistry ◽

10.1155/2021/7112400 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Taksid Charasseangpaisarn ◽

Pattarawadee Krassanairawiwong ◽

Chanidapa Sangkanchanavanich ◽

Atima Kurjirattikan ◽

Kanyarak Kunyawatyuwapong ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Sodium Hydroxide Solution ◽

Resin Composite ◽

In Vitro Study ◽

Lithium Disilicate ◽

Surface Element ◽

Similar Amount ◽

Modes Of Failure

Background and Purpose. Contamination of the lithium disilicate (LDS) during the try-in procedure is unavoidable and may weaken the bond strength of restoration. The purpose of this study was to investigate the efficacy of different surface cleansing agents on the shear bond strength (SBS) of contaminated LDS. Materials and Methods. Seventy LDS specimens were randomly divided into seven groups. The first group was noncontaminated surface (PC). The six other groups were contaminated with the saliva and silicone disclosing medium and treated with no surface cleansing agent (NC); phosphoric acid (PO); Ivoclean (IV); sodium hydroxide solution (NA); Restorative Cleansing Agent (RC); and hydrofluoric acid (HF). Then, LDS specimens were cementated with Panavia V5 to resin composite rod. Each specimen was subjected to an SBS test. The modes of failure was inspected under light microscope. The surface element of each group was examined by SEM-EDS. Results. The results were analyzed with one-way ANOVA and Tamhane’s T2. The mean SBS value of NC was significantly lower than others ( p < 0.05 ), and HF was significantly higher than others ( p < 0.05 ). However, PC, PO, IV, NA, and RC were not significantly different from each other ( p > 0.05 ). The mode of failure was mostly adhesive failure in every group. The surface showed similar amount of elements in every group. Conclusions. The SBS of LDS was reduced by saliva and silicone disclosing medium contamination which can be restored using acid- and alkaline-based surface cleansing agents before the cementation procedure.

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In Vitro Study on the Effect of a New Bioactive Desensitizer on Dentin Tubule Sealing and Bonding

Journal of Functional Biomaterials ◽

10.3390/jfb11020038 ◽

2020 ◽

Vol 11 (2) ◽

pp. 38

Author(s):

Minh N. Luong ◽

Laurie Huang ◽

Daniel C. N. Chan ◽

Alireza Sadr

Keyword(s):

Bond Strength ◽

In Vitro Study ◽

Vitro Study ◽

Acid Pretreatment ◽

Universal Adhesive ◽

Cross Sectional ◽

Before And After ◽

Human Dentin ◽

Microshear Bond Strength

Bioactive mineral-based dentin desensitizers that can quickly and effectively seal dentinal tubules and promote dentin mineralization are desired. This in vitro study evaluated a novel nanohydroxyapatite-based desensitizer, Predicta (PBD, Parkell), and its effect on bond strength of dental adhesives. Human dentin discs (2-mm thick) were subjected to 0.5 M EDTA to remove the smear layer and expose tubules, treated with PBD, and processed for surface and cross-sectional SEM examination before and after immersion in simulated body fluid (SBF) for four weeks (ISO 23317-2014). The effects of two dental desensitizers on the microshear bond strength of a universal adhesive and a two-step self-etch system were compared. SEM showed coverage and penetration of nanoparticles in wide tubules on the PBD-treated dentin at the baseline. After four weeks in SBF, untreated dentin showed amorphous mineral deposits while PBD-treated dentin disclosed a highly mineralized structure integrated with dentin. Desensitizers significantly reduced microshear bond strength test (MSBS) of adhesives by 15–20% on average, depending on the bonding protocol. In conclusion, PBD demonstrated effective immediate tubules sealing capability and promoted mineral crystal growth over dentin and into the tubules during SBF-storage. For bonding to desensitizer-treated dentin, a two-step self-etching adhesive or universal bond with phosphoric acid pretreatment are recommended.

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