scholarly journals Chlorhexidine Preserves Dentin Bond in vitro

2007 ◽  
Vol 86 (1) ◽  
pp. 90-94 ◽  
Author(s):  
M.R.O. Carrilho ◽  
R.M. Carvalho ◽  
M.F. de Goes ◽  
V. di Hipólito ◽  
S. Geraldeli ◽  
...  
Keyword(s):  
Bond Strength ◽  
Protease Inhibitors ◽  
Resin Composite ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Hybrid Layer ◽  
Adhesive Resin ◽  
Bond Stability ◽  
Etch And Rinse

Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted third molars were sectioned into two halves. One half was customarily restored (etch-and-rinse adhesive/resin composite), and the other was treated with 2% chlorhexidine after being acid-etched before restoration. Specimens were stored in artificial saliva with/without protease inhibitors. Microtensile bond strengths and failure mode distribution under SEM were analyzed immediately after specimens’ preparation and 6 months later. With chlorhexidine, significantly better preservation of bond strength was observed after 6 months; protease inhibitors in the storage medium had no effect. Failure analysis showed significantly less failure in the hybrid layer with chlorhexidine, compared with controls after 6 months. In conclusion, this in vitro study suggests that chlorhexidine might be useful for the preservation of dentin bond strength.

scholarly journals In Vitro Evaluation of Benzalkonium Chloride in the Preservation of Adhesive Interfaces

2014 ◽  
Vol 39 (3) ◽  
pp. 283-290 ◽  
Author(s):  
C Sabatini ◽  
JH Kim ◽  
P Ortiz Alias
Keyword(s):  
Bond Strength ◽  
Mass Loss ◽  
Benzalkonium Chloride ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Control Group ◽  
Significance Level ◽  
Bond Stability ◽  
Etch And Rinse

SUMMARY Inhibition of endogenous dentin matrix metalloproteinases (MMPs) by benzalkonium chloride (BAC) decreases collagen solubilization and may help improve resin-dentin bond stability. Objective: This study evaluated the resin-dentin bond stability of experimental adhesive blends containing BAC and the stability of dentin matrices by assessing the mass loss and collagen solubilization from dentin beams pretreated with BAC. Materials and Methods Twenty-five healthy molars were used for the bond strength evaluation of a two-step etch-and-rinse adhesive (Adper Single Bond Plus, SB) modified with BAC or not. The following groups were tested: 1) SB with no inhibitor (control); 2) topical 2.0% chlorhexidine + SB; 3) 1.0% BAC etchant + SB; 4) 0.5% BAC-SB; and 5) 1.0% BAC-SB. Microtensile bond strength (μTBS) and failure mode distribution under standard error of the mean were evaluated after 24 hours and six months of storage in artificial saliva (AS). A two-way analysis of variance and Tukey test with a significance level of p<0.05 was used for data analysis. In addition, 30 completely demineralized dentin beams from human molars were either dipped in deionized water (DW, control) or dipped in 0.5% and 1.0% BAC for 60 seconds, and then incubated in AS. Collagen solubilization was assessed by evaluating the dry mass loss and quantifying the amount of hydroxyproline (HYP) released from hydrolyzed specimens after four weeks of incubation. Results The control group demonstrated lower μTBS than some of the experimental groups containing BAC at 24 hours and six months (p<0.05). When BAC was incorporated into the adhesive blend in concentrations of 0.5% and 1.0%, no reduction in dentin bond strength was observed after six months (p<0.05). Less mass loss and HYP release was seen for dentin matrices pretreated with BAC relative to the control pretreated with DW (p<0.05). Conclusion This in vitro study demonstrates that BAC contributes to the preservation of resin-dentin bonds by reducing collagen degradation.

scholarly journals Evaluation of Caries-Free Restorations Bonded with Various Adhesive Systems: In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Morakot Piemjai ◽  
Pareewan Chantarawej ◽  
Nobuo Nakabayashi
Keyword(s):  
Resin Composite ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Negative Control ◽  
Adhesive Systems ◽  
Hybrid Layer ◽  
Class V ◽  
Secondary Caries ◽  
Dye Penetration

Purpose. Secondary caries originate from a leakage pathway where oral acids can penetrate faster and demineralize the tooth substrate deeper which can be visualized by dye penetration. The ability to prevent secondary caries by contemporary adhesive systems was evaluated in this study. Dye penetration distance through leakage and into the tooth substrate adjacent to Class V restorations after artificial caries exposure was compared. Materials and Methods. Previously frozen extracted human molars were used to prepare the Class V cavities at the CEJ on axial surfaces. All cavities were restored with either the resin-composite or amalgam with or without resin adhesives: dry bonding: Super-Bond D-Liner II Plus; moist bonding: All-Bond 2; and self-etch bonding: AQ Bond and Clearfil Protect Bond. Two subgroups of Super-Bond D-Liner II Plus were immersed for 14 days at 37°C either in artificial saliva (negative control) or the artificial caries solution. The other groups were soaked in the artificial caries solution. The distance of dye penetration into the adjacent enamel, cementum/dentin, and tooth-resin interfaces was measured after immersion in 0.5% basic fuchsin dye for 24 h. The tooth-resin interfacial layer was investigated using SEM. Results. No dye penetration into the tooth-resin interface was found in Super-Bond D-Liner II Plus and AQ Bond groups which demonstrated a constant hybrid layer after a chemical challenge. The leakage distance at the cementum/dentin-resin interface of All-Bond 2, Clearfil Protect Bond, and non-adhesive amalgam (positive control) groups was significantly higher than the distance of dye penetration into the adjacent demineralized root surface (p<0.05). Conclusion. Caries associated with either amalgam or resin-composite restorations can be prevented using resin adhesives which can penetrate into the intact tooth substrate to form a stable hybrid layer. With caries-free restorations, tooth vitality may be conserved lifelong.

scholarly journals Riboflavin and Its Effect on Dentin Bond Strength: Considerations for Clinical Applicability—An In Vitro Study

2022 ◽  
Vol 9 (1) ◽  
pp. 34
Author(s):  
Franziska Beck ◽  
Nicoleta Ilie
Keyword(s):  
Bond Strength ◽  
Resin Composite ◽  
In Vitro Study ◽  
Dental Resin ◽  
Hybrid Layer ◽  
Clinical Applicability ◽  
Human Dentin ◽  
Post Hoc ◽  
Self Etch

Bioactive collagen crosslinkers propose to render the dentin hybrid layer less perceptive to hydrolytic challenge. This study aims to evaluate whether bond strength of dental resin composite to dentin benefits from riboflavin (RB)-sensitized crosslinking when used in a clinically applicable protocol. A total of 300 human dentin specimens were prepared consistent with the requirements for a macro-shear bond test. RB was applied on dentin, either incorporated in the primer (RBp) of a two-step self-etch adhesive or as an aqueous solution (RBs) before applying the adhesive, and blue light from a commercial polymerization device was used for RB photoactivation. Bonding protocol executed according to the manufacturer’s information served as control. Groups (n = 20) were tested after 1 week, 1 month, 3 months, 6 months or 1 year immersion times (37 °C, distilled water). The different application methods of RB significantly influenced bond strength (p < 0.001) with a medium impact (η2p = 0.119). After 1 year immersion, post hoc analysis identified a significant advantage for RB groups compared to RBp (p = 0.018), which is attributed to a pH-/solvent-dependent efficiency of RB-sensitized crosslinking, stressing the importance of formulation adjustments. We developed an application protocol for RB-sensitized crosslinking with emphasis on clinical applicability to test its performance against a gold-standard adhesive, and are confident that, with a few adjustments to the application solution, RB-sensitized crosslinking can improve the longevity of adhesive restorations in clinics.

scholarly journals Comparison of the Shear Bond Strength of Acid Etched and Er,Cr:YSGG Laser Irradiated IPS Empress II and Feldspathic Ceramic Surfaces to Metal Brackets: an in-Vitro Study

2021 ◽  
Vol 12 (5) ◽  
pp. 6863-6872
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
In Vitro Study ◽  
Acid Etching ◽  
Adhesive Resin ◽  
Ceramic Surfaces ◽  
Significant Difference

In this experimental study, the effects of Er,Cr:YSGG laser on the shear bond strength (SBS) of brackets bonded to two ceramic surfaces (Feldspathic and IPS Empress II) were compared to hydrofluoric (HF) acid etching. 60 ceramic discs were prepared, including two groups: Feldspathic and IPS Empress II surfaces. In each group, the following subgroups were prepared: 2W Er,Cr:YSGG laser, 2.5W Er,Cr:YSGG laser, and HF acid-etching. Ceramic primer and adhesive resin composite were applied and then light-cured to bond the ceramic cylinders to brackets. The samples were stored in distilled water for 24h and then, thermocycled. SBS testing was performed with a universal testing machine. Two-way ANOVA was used to compare SBS among subgroups (P<0.05). The highest SBS was seen in feldspathic surfaces with HF preparation (14.61±3.11MPa). There was a significant difference in SBS between different surface treatments except between the laser groups of IPS Empress II. Also, feldspathic surfaces had significantly higher SBS than IPS Empress II in all groups except HF. Conventional surface treatment provides much stronger adhesion than laser regardless of the ceramic type. Er,Cr:YSGG laser is effective on the SBS of the feldspathic ceramics, but not in IPS Empress II ceramics. So, laser treatment can be recommended as a favorable setting for treating feldspathic surfaces.

scholarly journals Micro Push-Out Bond Strength of Resin Composite to Dentin in Primary Dentition Using Three Universal Adhesives with Different pH: An In Vitro Study

2021 ◽  
Vol 11 (14) ◽  
pp. 6349
Author(s):  
Regina María Del Río Carrillo ◽  
Pedro Molinero-Mourelle ◽  
Vicente Vera ◽  
Pablo Romero Villaba ◽  
Elizabeth Casañas ◽  
...  
Keyword(s):  
Bond Strength ◽  
Resin Composite ◽  
In Vitro Study ◽  
Primary Dentition ◽  
Vitro Study ◽  
Deciduous Tooth ◽  
Universal Adhesives ◽  
Type Of Failure ◽  
Push Out

To evaluate the bond strength of different universal adhesives on deciduous tooth dentineand their relationship with the composition and potential of hydrogen (pH). Methods: An in vitromicro push-out test on 150 samples (n = 50) per group per adhesive, namely, Adhese Universal(ADH; Ivoclar Vivadent), Futurabond U (FUT; Voco GmbH) (Test), and Scotchbond Universal withpre-conditioning (SCO; 3M) (Control), to record bond strength (BS) and type of adhesive failure.Results: The results of the different adhesives (megapascals (MPa)) varied, showing no statisticalsignificance. The corresponding averages are in MPa: ADH, 13.66 2.81; FUT, 14.48 2.88; SCO,14.98 3.96. Additionally, the frequency of type of failure was as follows: mixed (60.7%), adhesive(27.3%), and cohesive (12%). Conclusions: SCO, with a pH of 2.7, showed greater resistance tofracture, while FUT, with a pH of 2.3 and no pre-conditioning, approached the same values, being aone-step adhesive. No relationship was found between failure and type of adhesive.

scholarly journals Effect of topical fluoride on microshear bond strength of primary enamel to composite, microhardness of enamel and its surface morphology: An in vitro study

2019 ◽  
Vol 13 (4) ◽  
pp. 305-310
Author(s):  
Mina Biria ◽  
Sajedeh Namaei Ghasemi ◽  
Seyedeh Mahsa Sheikh-Al-Eslamian ◽  
Narges Panahandeh
Keyword(s):  
Bond Strength ◽  
Artificial Saliva ◽  
Morphological Characteristics ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Modes Of Failure ◽  
Group 3 ◽  
Microshear Bond Strength

Background. This in vitro study aimed to evaluate the microshear bond strength (μSBS), microhardness and morphological characteristics of primary enamel after treating with sodium fluoride (NaF) and acidulated phosphate fluoride (APF). Methods. Forty-eight primary canines were cut into mesial and distal sections and assigned to five groups randomly: group 1 (immersed in saliva as a control), group 2 (treated with NAF and immersed in saliva for 30 minutes), group 3 (treated with APF and immersed in saliva for 30 minutes), group 4 (treated with NAF and immersed in saliva for 10 days), and group 5 (treated with APF and immersed in saliva for 10 days). Composite resin (Filtek Z250) was bonded on the specimens (n=15) for measuring the μSBS. After storage in 37°C artificial saliva for 24 hours, µSBS and Vickers hardness tests (10 readings) were performed. The data were analyzed using one-way ANOVA and Kolmogorov-Smirnov, Levene’s and Tukey HSD tests (P<0.05). Morphological analysis of enamel and modes of failure were carried out under a scanning electron microscope (SEM) on two remaining specimens. Results. Significant differences in μSBS were only noted between groups 2 and 4 (P=0.024). Group 3 showed a significant decrease in hardness after storage in artificial saliva (P<0.001), with a significantly lower hardness than the other groups (P<0.001). The SEM observations showed irregular particles in groups 3 and 5; uniform, smooth and thin coats were seen in groups 2 and 4. Conclusion. Fluoride therapy with NaF and APF gels prior to restorative treatments had no adverse effects on the microshear bond strength.

scholarly journals COMPARATIVE EVALUATION OF THE SHEAR BOND STRENGTH OF THREE SELF-ETCH DENTIN BONDING AGENTS TO DENTIN: AN IN-VITRO STUDY

2020 ◽  
Vol 8 (10) ◽  
pp. 454-459
Author(s):  
Bhalla V. ◽  
◽  
K. Goud M. ◽  
Chockattu S. ◽  
Khera A ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Group Analysis ◽  
In Vitro Study ◽  
Bonding Agents ◽  
Mandibular Molar ◽  
Self Etch

Background:Dentin bonding is an ever-evolving field in adhesive dentistry. With the introduction of newer systems into the market, there is a crucial need to test their efficiency in terms of bond strength. Dual-cured adhesives in theory may provide for a better degree of conversion as compared to conventional light-cured adhesives .Thus, the aim of this study was to compare the shear bond strength of three different self-etch adhesives namely ClearfilSE bond (Kuraray), Tetric N Bond Universal (IvoclarVivadent) and Futura Bond DC (Voco) to dentin. Materials & Methods: Ninety extracted non-carious, intact human mandibular molar teeth were selected for this study. Each tooth was decoronated using a double-sided diamond disc with water coolant to a depth of 2mm from the cusp tip .The cut dentin surface was then abraded against 600-grit wet silicon carbide papers for 60 seconds to produce a uniform smear layer. The root portion of each tooth was mounted on a plastic ring using cold cure acrylic resin. Specimens were then divided into three adhesive groups of 30 teeth each, Group A: ClearfilSE Bond (Kuraray), Group B: Tetric N Bond Universal (IvoclarVivadent), Group C :Futura Bond DC (Voco). All bonding agents were used according to the manufacturers’ instructions, in combination with the resin composite Tetric N Ceram (IvoclarVivadent). The samples were thermocycled, followed by shear bond strength testing using a Universal testing machine (Hounsfield). Data were subjected to statistical analysis using one-way analysis of variance (ANOVA) (P<0.05) and Post hoc Tukey’s test for inter- and intra- group analysis respectively. Results: Clearfil SE Bond yielded the highest shear bond strength values (30.9 ±4.66 MPa) which were statistically significant, followed byTetric N Bond Universal group (29.8 ±4.34) and the lowest shear bond strength values were recorded for Futura Bond DC (18.2 ±3.13). Conclusion: Clearfil SE bond and Tetric N bond Universal can be considered as better options than Futura Bond DC.

scholarly journals The Influence of Different Surface Cleansing Agents on Shear Bond Strength of Contaminated Lithium Disilicate Ceramic: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Taksid Charasseangpaisarn ◽  
Pattarawadee Krassanairawiwong ◽  
Chanidapa Sangkanchanavanich ◽  
Atima Kurjirattikan ◽  
Kanyarak Kunyawatyuwapong ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Sodium Hydroxide Solution ◽  
Resin Composite ◽  
In Vitro Study ◽  
Lithium Disilicate ◽  
Surface Element ◽  
Similar Amount ◽  
Modes Of Failure

Background and Purpose. Contamination of the lithium disilicate (LDS) during the try-in procedure is unavoidable and may weaken the bond strength of restoration. The purpose of this study was to investigate the efficacy of different surface cleansing agents on the shear bond strength (SBS) of contaminated LDS. Materials and Methods. Seventy LDS specimens were randomly divided into seven groups. The first group was noncontaminated surface (PC). The six other groups were contaminated with the saliva and silicone disclosing medium and treated with no surface cleansing agent (NC); phosphoric acid (PO); Ivoclean (IV); sodium hydroxide solution (NA); Restorative Cleansing Agent (RC); and hydrofluoric acid (HF). Then, LDS specimens were cementated with Panavia V5 to resin composite rod. Each specimen was subjected to an SBS test. The modes of failure was inspected under light microscope. The surface element of each group was examined by SEM-EDS. Results. The results were analyzed with one-way ANOVA and Tamhane’s T2. The mean SBS value of NC was significantly lower than others ( p < 0.05 ), and HF was significantly higher than others ( p < 0.05 ). However, PC, PO, IV, NA, and RC were not significantly different from each other ( p > 0.05 ). The mode of failure was mostly adhesive failure in every group. The surface showed similar amount of elements in every group. Conclusions. The SBS of LDS was reduced by saliva and silicone disclosing medium contamination which can be restored using acid- and alkaline-based surface cleansing agents before the cementation procedure.

scholarly journals Adhesion of Two Bonding Systems to Air-Abraded or Bur-Abraded Human Enamel Surfaces

2008 ◽  
Vol 02 (03) ◽  
pp. 167-175 ◽  
Author(s):  
Abdulkadir Sengun ◽  
Hasan Orucoglu ◽  
Ilknur Ipekdal ◽  
Fusun Ozer
Keyword(s):  
Bond Strength ◽  
Composite Resin ◽  
In Vitro Study ◽  
Air Abrasion ◽  
Adhesive Resin ◽  
Bonding Performance ◽  
Human Enamel ◽  
Bond Strengths ◽  
Bonding Systems

ABSTRACTObjectives: The purpose of this in vitro study was to evaluate whether mechanical alteration of the enamel surfaces with air abrasion and bur abrasion techniques could enhance the bonding performance of a three step and a self etching adhesive resin systems to enamel.Methods: 126 extracted lower human incisor teeth were used. The teeth were divided into three groups including 40 teeth each. First group; teeth were used as control and no preparation was made on enamel surfaces, 2nd group; outer enamel surfaces were air abraded, 3rd group; outer enamel surfaces were abraded mechanically with a diamond fissure bur. Cylinder composite resin blocks were bonded to the buccal enamel surfaces with two bonding systems (20 specimens in each group). Bond strengths to enamel surfaces were measured at a cross-head speed of 1 mm/min. The data were analyzed by ANOVA and Duncan Tests. To examine interface composite resin/enamel surfaces at scanning electron microscopy, remaining 6 teeth were used. Fracture analysis was performed using an optical stereomicroscope.Results: Bond strengths values of Solid Bond were significantly higher than bond strengths of Clearfil SE Bond for all types of enamel (P<.05). Shear bond strength values obtained with Solid Bond (three step system) to three types of enamel surfaces [air-abraded (30.25±7.00 MPa), bur-abraded (29.07±3.53 MPa), control (31.74±7.35 MPa)] were close to each other (P>.05). The macroscopic mode of failures for bonding systems, SB and SE Bond appeared to be adhesive and cohesive in nature.Conclusions: In order to get better bond strength values with self etching systems, it is advisable to prepare enamel surfaces with bur or air abrasion, but it enamel preparation is not necessary for three step systems. (Eur J Dent 2008;2:167-175)

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