scholarly journals Circadian Clock Gene Period Contributes to Diapause via GABAeric-Diapause Hormone Pathway in Bombyx mori

Biology ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 842
Author(s):  
Wen-Zhao Cui ◽  
Jian-Feng Qiu ◽  
Tai-Ming Dai ◽  
Zhuo Chen ◽  
Jiang-Lan Li ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Bombyx Mori ◽  
Clock Gene ◽  
Endocrine System ◽  
Circadian Clock Gene ◽  
Seasonal Adaptation ◽  
Clock System ◽  
Effects Of Temperature ◽  
Diapause Hormone ◽  
Per Gene

Diapause is a developmental transition in insects based on seasonal adaptation to adversity; it is regulated by a circadian clock system and the endocrine system. However, the molecular node and its mechanism underlying the effects of these systems are still unclear. Here, a mutant of Bombyx mori with the circadian clock gene Period (Per) knocked out was constructed, which dramatically changed the classic diapause-destined pathway. Per-knockout silkworms powerfully attenuated, but could not completely block, the predetermined effects of temperature and photoperiod on diapause determination, and this effect depended on the diapause hormone (DH) pathway. The impaired transcription-translation feedback loop of the circadian clock system lacking the Per gene caused direct up-regulation of the expression of GRD, a receptor of γ-aminobutyric acid (GABA), by changing expression level of Cycle. The synthesis of GABA in the tissue complex of brain-suboesophageal ganglion then increased and restricted the decomposition, which continuously promoted the GABAergic signal to play a role, and finally inhibiting (delaying) the release of DH to the hemolymph, and reducing the diapause-inducing effect of DH. The results provided an example to explain the regulatory mechanism of the circadian clock on endocrine hormones in the silkworm.

scholarly journals The BrGI Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1664
Author(s):  
Nan-Sun Kim ◽  
Su-Jeong Kim ◽  
Jung-Su Jo ◽  
Jun-Gu Lee ◽  
Soo-In Lee ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Circadian Clock ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Clock Gene ◽  
Clock Genes ◽  
Floral Induction ◽  
Stem Elongation ◽  
Circadian Clock Gene ◽  
Clock System

Circadian clocks integrate environmental cues with endogenous signals to coordinate physiological outputs. Clock genes in plants are involved in many physiological and developmental processes, such as photosynthesis, stomata opening, stem elongation, light signaling, and floral induction. Many Brassicaceae family plants, including Chinese cabbage (Brassica rapa ssp. pekinensis), produce a unique glucosinolate (GSL) secondary metabolite, which enhances plant protection, facilitates the design of functional foods, and has potential medical applications (e.g., as antidiabetic and anticancer agents). The levels of GSLs change diurnally, suggesting a connection to the circadian clock system. We investigated whether circadian clock genes affect the biosynthesis of GSLs in Brassica rapa using RNAi-mediated suppressed transgenic Brassica rapa GIGENTEA homolog (BrGI knockdown; hereafter GK1) Chinese cabbage. GIGANTEA plays an important role in the plant circadian clock system and is related to various developmental and metabolic processes. Using a validated GK1 transgenic line, we performed RNA sequencing and high-performance liquid chromatography analyses. The transcript levels of many GSL pathway genes were significantly altered in GK1 transgenic plants. In addition, GSL contents were substantially reduced in GK1 transgenic plants. We report that the BrGI circadian clock gene is required for the biosynthesis of GSLs in Chinese cabbage plants.

scholarly journals Phenotypic effects induced by knock-down of the period clock gene in Bombyx mori

2007 ◽  
Vol 89 (2) ◽  
pp. 73-84 ◽  
Author(s):  
FEDERICA SANDRELLI ◽  
SILVIA CAPPELLOZZA ◽  
CLARA BENNA ◽  
ALESSIO SAVIANE ◽  
ANTONIO MASTELLA ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Clock Gene ◽  
Developmental Time ◽  
Economic Importance ◽  
Circadian Clock Gene ◽  
Egg Hatching ◽  
Knock Down ◽  
Production Parameters ◽  
Silk Production ◽  
Practical Level

SummaryThe lepidopteran Bombyx mori is an insect of considerable scientific and economic importance. Recently, the B. mori circadian clock gene period has been molecularly characterized. We have transformed a B. mori strain with a construct encoding a period double-strand RNA in order to knock-down period gene expression. We observe that this post-transcriptional silencing produces a small but detectable disruption in the egg-hatching rhythm, as well as a reduction in egg-to-adult developmental time, without altering silk production parameters. Thus we show that both circadian and non-circadian phenotypes can be altered by changing per expression, and, at a practical level, these results suggest that per knock-down may provide a suitable strategy for improving the efficiency of rearing, without affecting silk productivity.

scholarly journals Effect of Circadian Clock Gene Mutations on Nonvisual Photoreception in the Mouse

2012 ◽  
Vol 53 (1) ◽  
pp. 454 ◽  
Author(s):  
Leah Owens ◽  
Ethan Buhr ◽  
Daniel C. Tu ◽  
Tamara L. Lamprecht ◽  
Janet Lee ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Clock Gene ◽  
Gene Mutations ◽  
Circadian Clock Gene
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