scholarly journals Biological Activity of Humulus lupulus (L.) Essential Oil and Its Main Components against Sitophilus granarius (L.)

Biomolecules ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1108
Author(s):  
Gianluca Paventi ◽  
Laura de Acutis ◽  
Antonio De Cristofaro ◽  
Marco Pistillo ◽  
Giacinto S. Germinara ◽  
...  
Keyword(s):  
Biological Activity ◽  
Essential Oil ◽  
Biologically Active ◽  
Contact Toxicity ◽  
Inhalation Toxicity ◽  
Fumigant Toxicity ◽  
Sitophilus Granarius ◽  
Brewing Industry ◽  
Humulus Lupulus L ◽  
Main Components

Besides its use in the brewing industry, hop cones appear as a powerful source of biologically active compounds, already checked for their putative anticancer, antimicrobial, and other bioactivities. Conversely, hop use in pest control remains to date under-investigated. Therefore, the biological activity of hop essential oil (EO) and its main constituents was investigated here against Sitophilus granarius. Adult contact toxicity was found 24 h after treatment with hop EO (LD50/LD90 13.30/40.23 µg/adult), and its three most abundant components, α-humulene, β-myrcene, and β-caryophyllene (LD50/LD90 41.87/73.51, 75.91/126.05, and 138.51/241.27 µg/adult, respectively); negligible variations at 48 h, except for α-humulene (LD50/LD90 26.83/49.49 µg/adult), were found. The fumigant toxicity of the EO and terpenes was also checked: in the absence of wheat grains, β-myrcene showed the highest inhalation toxicity (LC50/LC90 72.78/116.92 mg/L air), whereas α-humulene, β-caryophyllene, and the EO induced similar values (LC50/LC90 about 130/200 mg/L air); with the exception for EO, the wheat presence increased (30–50%) LC50/LC90 values. Moreover, EO and terpenes were perceived by insect antennae and elicited repellent activity. Only β-caryophyllene showed an anticholinesterase effect, this suggesting that different mechanisms of action should be responsible for hop EO toxicity. Therefore, hop EO appears suitable for developing control means against this pest.

Analysis of the Essential Oil of Illicium henryi Diels Root Bark and Its Insecticidal Activity against Liposcelis bostrychophila Badonnel

2015 ◽  
Vol 78 (4) ◽  
pp. 772-777 ◽  
Author(s):  
XIN CHAO LIU ◽  
ZHI LONG LIU
Keyword(s):  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Root Bark ◽  
Liposcelis Bostrychophila ◽  
Stored Grains ◽  
Natural Insecticides ◽  
Main Components ◽  
Total Oil

Water-distilled essential oil from Illicium henryi (Illiciaceae) root bark was analyzed by gas chromatography–mass spectrometry. Thirty-four compounds, accounting for 97.86% of the total oil, were identified. The main components of the essential oil of I. henryi root bark were safrole (46.12%), myristicin (20.39%), and 1,8-cineole (6.17%), followed by α-cadinol (3.784%) and linalool (3.22%). The essential oil had higher levels of phenylpropanoids (66.89%) than of monoterpenoids (14.83%) and sesquiternoids (16.14%). Three constituents were isolated from the oil based on bioactivity fractionation. The essential oil possessed fumigant toxicity against booklice (Liposcelis bostrychophila), with a 50% lethal concentration (LC50) of 380.39 μg/liter of air, while the two isolated constituents myristicin and safrole had LC50s of 121.95 and 322.54 μg/liter, respectively. Another constituent, 1,8-cineole, showed weaker toxicity, with an LC50 of 1,120.43 μg/liter. The essential oil also exhibited contact toxicity against L. bostrychophila, with an LC50 of 96.83 μg/cm2. Myristicin (LC50, 18.74 μg/cm2) and safrole (LC50, 69.28 μg/cm2) exhibited stronger acute toxicity than 1,8-cineole (LC50, 1,049.41 μg/cm2) against the booklice. The results indicated that the essential oil and its constituent compounds have potential for development into natural insecticides for control of psocids in stored grains.

scholarly journals Chemical constituents and biological activities against Tribolium castaneum (Herbst) of the essential oil from Citrus wilsonii leaves

2014 ◽  
Vol 79 (10) ◽  
pp. 1213-1222 ◽  
Author(s):  
Hai Chen ◽  
Kai Yang ◽  
Chun You ◽  
Shu Du ◽  
Qian Cai ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Positive Control ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Active Compounds ◽  
Active Constituents ◽  
Main Components

The essential oil obtained from Citrus wilsonii Tanaka leaves with hydrodistillation was investigated by GC and GC-MS. The main components of the essential oil were identified to be citronellol (16.94%), nerol acetate (10.35%), ?-terpinen (9.85%), citronellal (9.36%) and ?-pinene (6.72%). Among them, the four active constituents, predicted with a bioactivity-test, were isolated and identified as citronellol, ?-terpinene, nerol (neryl) acetate and ?-pinene. It was found that the essential oil of C. wilsonii leaves and isolated compounds possessed fumigant and contact toxicity against Tribolium castaneum adults. The essential oil and ?-terpinen showed strong fumigant toxicity against T. castaneum (LC50 = 8.18 and 4.09 mg L-1). Repellency of the crude oil and active compounds was also determined. Citronellol, neryl acetate and ?-pinene were strongly repellent (100%, 86% and 92%, respectively, at 78.63 nL cm-2, after 2 h treatment) against T. castaneum. The essential oil and citronellol exhibited the same level of repellency compared with the positive control, DEET. The results indicate that the essential oil of C. wilsonii leaves and its active compounds had the potential to be developed as natural fumigants, insecticides and repellents for control of T. castaneum.

Chemical Composition and Insecticidal Activities of the Essential Oil of Clinopodium chinense (Benth.) Kuntze Aerial Parts against Liposcelis bostrychophila Badonnel

2015 ◽  
Vol 78 (10) ◽  
pp. 1870-1874 ◽  
Author(s):  
HENG YU LI ◽  
XIN CHAO LIU ◽  
XU BO CHEN ◽  
QI ZHI LIU ◽  
ZHI LONG LIU
Keyword(s):  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Bornyl Acetate ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Lc50 Value ◽  
Liposcelis Bostrychophila ◽  
Aerial Parts ◽  
Main Components ◽  
Total Oil

Water-distilled essential oil from Clinopodium chinense (Labiatae) aerial parts at the flowering stage was analyzed by gas chromatography–mass spectrometry. Thirty-five compounds, accounting for 99.18% of the total oil, were identified, and the main components of the essential oil of C. chinense were spathulenol (18.54%), piperitone (18.9%), caryophyllene (12.04%), and bornyl acetate (8.14%). Based on bioactivity-directed fractionation, bornyl acetate, caryophyllene, and piperitone were identified from the essential oil. The essential oil possessed fumigant toxicity against booklice (Liposcelis bostrychophila) with a 50% lethal concentration (LC50) value of 423.39 μg/liter, while the isolated constituents, bornyl acetate and piperitone, had LC50 values of 351.69 and 311.12 μg/liter against booklice, respectively. The essential oil also exhibited contact toxicity against L. bostrychophila with an LC50 value of 215.25 μg/cm2. Bornyl acetate, caryophyllene, and piperitone exhibited acute toxicity against booklice with LC50 values of 321.42, 275.00, and 139.74 μg/cm2, respectively. The results indicated that the essential oil and its isolated constituents have potential for development into natural insecticides or fumigants for control of insects in stored grains.

scholarly journals Bioactivity of Wild Hop Extracts against the Granary Weevil, Sitophilus granarius (L.)

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 564
Author(s):  
Gianluca Paventi ◽  
Giuseppe Rotundo ◽  
Marco Pistillo ◽  
Ilaria D’Isita ◽  
Giacinto Salvatore Germinara
Keyword(s):  
Pest Control ◽  
Anticholinesterase Activity ◽  
Contact Toxicity ◽  
Inhalation Toxicity ◽  
Sitophilus Granarius ◽  
Granary Weevil ◽  
Humulus Lupulus L ◽  
Interesting Species ◽  
Fumigation Toxicity

The use of bioinsecticides, rather than synthetic compounds, appears a goal to be pursued in pest control, especially for species such as Sitophilus granarius (L.) which attack stored products. Since Humulus lupulus (L.) is a remarkable source of bioactive compounds, this study investigated the bioactivity of hop flower extracts against S. granarius adults by evaluating toxic (contact, inhalation, and ingestion), repellent, antifeedant, and nutritional effects as well as their anticholinesterase activity and olfactory sensitivity. Hop extracts were obtained by soaking dried and ground hop cones in solvents of decreasing polarity: methanol, acetone, and n-hexane. Dried crude extracts were resuspended in each solvent, and used in topical application, ingestion, and fumigation toxicity assays, as well as in contact and short-range repellency tests, in vitro anticholinesterase activity evaluation, and electroantennographic tests. No inhalation toxicity for the extracts was found. On the contrary, all extracts showed adult contact toxicity 24 h after treatment (LD50/LD90 16.17/33.20, 25.77/42.64, and 31.07/49.48 µg/adult for acetone, n-hexane, and methanol extracts, respectively); negligible variations for these values at 48 h were found. The anticholinesterase activity shown by all extracts suggested that the inhibition of this enzyme was one of the mechanisms of action. Interestingly, flour disk bioassays revealed a significant ingestion toxicity for the acetone extract and a lower toxicity for the other two extracts. Moreover, all extracts affected insect nutritional parameters, at the highest dose checked. Filter paper and two-choice pitfall bioassays showed repellent activity and a strong reduction of insect orientation to a highly attractive food odor source, with minor differences among extracts, respectively. Finally, the presence of volatile compounds in the different extracts that are perceived by insect antennae was confirmed by electroantennography. All these findings strongly suggest a possible use of hop cone extracts against S. granarius, thus further confirming this plant as an interesting species for pest control.

scholarly journals Chemical Constituents and Insecticidal Activities of the Essential Oil ofCinnamomum camphoraLeaves againstLasioderma serricorne

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Hai Ping Chen ◽  
Kai Yang ◽  
Chun Xue You ◽  
Ning Lei ◽  
Rui Qi Sun ◽  
...  
Keyword(s):  
Essential Oil ◽  
Screening Program ◽  
Chemical Constituents ◽  
Wild Plants ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Cinnamomum Camphora ◽  
Chinese Medicinal Herbs ◽  
Lasioderma Serricorne ◽  
Main Components

During our screening program for agrochemicals from Chinese medicinal herbs and wild plants, the essential oil ofCinnamomum camphoraleaves was found to possess strong fumigant and contact toxicity againstLasioderma serricorneadults with LC50/LD50values of 2.5 mg/L air and 21.25 μg/adult, respectively. The essential oil obtained by hydrodistillation was investigated by GC and GC-MS. The main components of the essential oil were identified to beD-camphor (40.54%), linalool (22.92%), cineole (11.26%), and 3,7,11-trimethyl-3-hydroxy-6,10-dodecadien-1-yl acetate (4.50%). Bioactivity-directed chromatographic separation on repeated silica gel columns led to the isolation ofD-camphor and linalool.D-camphor and linalool showed strong fumigant toxicity (LC50= 2.36 and 18.04 mg/L air, resp.) and contact toxicity (LD50= 13.44 and 12.74 μg/adult, resp.) againstL. serricorne. The results indicate that the essential oil ofC. camphoraand its active compounds had the potential to be developed as natural fumigants and insecticides for control ofL. serricorne.

scholarly journals Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.

2019 ◽  
Vol 20 (2) ◽  
pp. 338-343 ◽  
Author(s):  
SHAHLA AMINI ◽  
MAJID GHORBANI NOHOOJI ◽  
MOUSA KHANI ◽  
MOHAMMAD REZA LABBAFI ◽  
FARAHNAZ KHALIGHI-SIGAROODI
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Sitophilus Oryzae ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Nepeta Cataria ◽  
Natural Substances

Amini S, Nohooji MG, Khani M, Labbafi MR, Khalighi-Sigaroodi f. 2019. Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.. Biodiversitas 20: 338-343. Recently, there has been a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known natural substances with insecticidal properties. These compounds may act as fumigants, contact insecticides, repellents, antifeedants and may affect growth and development of the insects. In an attempt to find a natural and inexpensive method for the control of stored-product pests, contact toxicity of essential oils of Nepeta cataria, Nepeta pogonosperma, Nepeta glomerulosa. and Nepeta binaloudensis. were investigated on adult insects of Sitophilus oryzae L. (Coleoptera, Curculionidae) in vitro condition. Chemical Composition, repellency and fumigant toxicity of the essential oils were investigated. Chemical composition of the essential oils of above plants were identified by GC-MS. 4aα,7α,7aβ-nepetalactone (82.74 %) was major component of N. cataria and also different isomers of nepetalactone were among the major compounds in N. Pogonosperma and N. binaloudensis oils. In fumigants bioassay, N. glomerulosa (124.318μL/L air) showed the highest toxicity against S. oryzae adults, followed by N.pogonosperma (150.49μL/L air) and N. cataria (152.630μL/L air), respectively. Also, the S. oryzae was repelled by N. cataria (91.61%) and N. binaloudensis (91.50 %), respectively.

scholarly journals Chemical Composition and Insecticidal Activity of the Essential Oil of Artemisia eriopoda against Maize Weevil, Sitophilus zeamais

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Guo Hua Jiang ◽  
Quan Ru Liu ◽  
Sha Sha Chu ◽  
Zhi Long Liu
Keyword(s):  
Essential Oil ◽  
Screening Program ◽  
Sitophilus Zeamais ◽  
Wild Plants ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Maize Weevil ◽  
Aerial Parts ◽  
Germacrene D ◽  
Main Components

In our screening program for new agrochemicals from local wild plants, Artemisia eriopoda was found to possess insecticidal activities against the maize weevil, Sitophilus zeamais. The essential oil of the aerial parts of A. eriopoda was obtained by hydrodistillation and investigated by GC and GC-MS. The main components of the essential oil are germacrene D (21.6%) and eucalyptol (14.2%). The essential oil possesses fumigant toxicity against S. zeamais adults with a LC50 value of 11.5 mg/L air and also shows contact toxicity against S. zeamais adults with a LD50 value of 24.8 μg/adult, respectively.

scholarly journals Contact Toxicity and Repellency of the Main Components From the Essential Oil ofClausena anisum-olensAgainst Two Stored Product Insects

2015 ◽  
Vol 15 (1) ◽  
pp. 87 ◽  
Author(s):  
Chun Xue You ◽  
Hai Yan Jiang ◽  
Wen Juan Zhang ◽  
Shan Shan Guo ◽  
Kai Yang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Contact Toxicity ◽  
Stored Product Insects ◽  
Main Components

Effect of Citrus reticulata Blanco Essential Oil on Cryptolestes ferrugineus (Stephens) Adults

2017 ◽  
Vol 80 (12) ◽  
pp. 2090-2093 ◽  
Author(s):  
Jianhua Lü
Keyword(s):  
Essential Oil ◽  
Insect Pest ◽  
Anhydrous Diethyl Ether ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Citrus Reticulata ◽  
Cryptolestes Ferrugineus ◽  
Fumigant Activity ◽  
Citrus Reticulata Blanco ◽  
Exposure Dosage

ABSTRACT The rusty grain beetle, Cryptolestes ferrugineus (Stephens), is a major insect pest of stored products worldwide. In an effort to manage populations of C. ferrugineus in stored grains, the essential oil from Citrus reticulata Blanco peel was prepared by the Soxhlet method with anhydrous diethyl ether, and its contact activity, repellent activity, and fumigant activity were investigated against C. ferrugineus adults. C. reticulata peel essential oil had potent contact, repellent, and fumigant activity against C. ferrugineus adults, with activity significantly increasing with increasing exposure dosage during the same exposure time. Percent repellency values achieved >80.0% after 60 h of exposure. For contact toxicity, the corrected mortality of C. ferrugineus adults reached >99.0% after 48 h of exposure at the dosage of 2.0 μL/cm2. For fumigant toxicity, the corrected mortality of C. ferrugineus adults reached 89.0 and 100.0% at the dosages of 40 and 80 μL/L of air, respectively. These results suggest that the C. reticulata peel essential oil has strong potential for managing populations of C. ferrugineus in practice.

scholarly journals Activity of Schinus Areira (Anacardiaceae) Essential Oils against the Grain Storage Pest Tribolium castaneum

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Lilian R. Descamps ◽  
Carolina Sánchez Chopa ◽  
Adriana A. Ferrero
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Feeding Deterrent ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Nutritional Index ◽  
Leaf Essential Oil ◽  
Storage Pest ◽  
Coleoptera Tenebrionidae

Essential oils extracted from leaves and fruits of Schinus areira (Anacardiaceae) were tested for their repellent, toxic and feeding deterrent properties against Tribolium castaneum (Coleoptera: Tenebrionidae) larvae and adults. A topical application assay was employed for the contact toxicity study and filter paper impregnation for the fumigant assay. A treated diet was also used to evaluate the repellent activity and a flour disk bioassay for the feeding deterrent action and nutritional index alteration. The essential oil of the leaves contained mainly monoterpenoids, with α-phellandrene, 3-carene and camphene predominant, whereas that from the fruits contained mainly α-phellandrene, 3-carene and β-myrcene. The leaf essential oil showed repellent effects, whereas that from the fruit was an attractant. Both oils produced mortality against larvae in topical and fumigant bioassays, but fumigant toxicity was not found against adults. Moreover, both essential oils produced some alterations in nutritional index. These results show that the essential oils from S. areira could be applicable to the management of populations of Tribolium castaneum.

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