scholarly journals Concentration Sensitivity of Nucleic Acid and Protein Molecule Detection Using Nanowire Biosensors

Biophysica ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 328-333
Author(s):  
Yuri D. Ivanov ◽  
Vadim Yu. Tatur ◽  
Alexander V. Glukhov ◽  
Vadim S. Ziborov
Keyword(s):  
Detection Limit ◽  
Nucleic Acid ◽  
Infectious Diseases ◽  
Early Stage ◽  
Protein Molecule ◽  
Cooperative Effect ◽  
Topical Issue ◽  
Low Concentration ◽  
Concentration Sensitivity ◽  
Concentration Detection Limit

The concentration detection limit (DL) of biomacromolecules attainable using a nanowire detector has become a topical issue. A DL of 10−15 M is required to reveal oncological and infectious diseases at an early stage. This study discusses the DL experimentally attainable in the subfemtomolar concentration range, and possible mechanisms explaining such a low-concentration DL through the cooperative effect of biomacromolecular complexes formed on the surface of the nanowire (NW) chip near the nanowire.

scholarly journals Nucleic Acid-based Diagnosis and Epidemiology of Infectious Diseases

10.5772/61965 ◽  
2016 ◽  
Author(s):  
Márcia Aparecida Sperança ◽  
Rodrigo Buzinaro Suzuki ◽  
Aline Diniz Cabral ◽  
Andreia Moreira dos Santos Carmo
Keyword(s):  
Nucleic Acid ◽  
Infectious Diseases

Fate of adsorbed arsenic during early stage sulfidization of nano-ferrihydrite

2019 ◽  
Vol 6 (7) ◽  
pp. 2228-2240 ◽  
Author(s):  
Guoqing Zhang ◽  
Danni Zhang ◽  
Zidan Yuan ◽  
Xu Ma ◽  
Lei Lei ◽  
...  
Keyword(s):  
Early Stage ◽  
Low Concentration

Sulfidization of As adsorbed nano-ferrihydrite at early stage by the low concentration of S(−ii) significantly affects the re-distribution and speciation of As, Fe, and S in the reducing environments.

scholarly journals Magnetic Particles for Advanced Molecular Diagnosis

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2158 ◽  
Author(s):  
Cristina Chircov ◽  
Alexandru Mihai Grumezescu ◽  
Alina Maria Holban
Keyword(s):  
Gene Expression ◽  
Infectious Disease ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Magnetic Particles ◽  
Early Stage ◽  
Disease Diagnosis ◽  
Laboratory Medicine ◽  
Clinical Reality

Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.

Nucleic acid vaccination strategies against infectious diseases

2015 ◽  
Vol 12 (12) ◽  
pp. 1851-1865 ◽  
Author(s):  
Małgorzata Anna Marć ◽  
Enrique Domínguez-Álvarez ◽  
Carlos Gamazo
Keyword(s):  
Nucleic Acid ◽  
Infectious Diseases ◽  
Vaccination Strategies

scholarly journals FLUORESCENT ANTIBODY DETECTION OF THE ANTIGENS OF THE SHOPE PAPILLOMA VIRUS IN PAPILLOMAS OF THE WILD AND DOMESTIC RABBIT

1957 ◽  
Vol 106 (4) ◽  
pp. 555-562 ◽  
Author(s):  
Wilbur Fiske Noyes ◽  
Robert C. Mellors
Keyword(s):  
Nucleic Acid ◽  
Early Stage ◽  
Fluorescent Antibody ◽  
Antibody Detection ◽  
Wild Rabbit ◽  
Papilloma Virus ◽  
Viral Antigens ◽  
Stage Of Development ◽  
Domestic Rabbit ◽  
Shope Papilloma Virus

The results obtained by a fluorescent antibody study of the Shope papilloma virus in papillomas of the wild and domestic rabbit are presented. In the wild rabbit papillomas the viral antigens occurred exclusively in the nucleus and were present in the differentiating cells of the keratohyaline layers and in the keratinized layers. The antigens were not present in the deeper proliferating epithelial cells of the papillomas. The Shope viral antigens were present in very minute amounts in papillomas of the domestic rabbit, as compared with papillomas of the wild rabbit, and were only detected in the superficial keratinized layers. It is postulated that virus is present in the nuclei of the proliferating cells of the papillomas of the wild and domestic rabbit but exists there in an early stage of development, consisting mainly of nucleic acid and deficient in protein, therefore non-antigenic and not demonstrable by fluorescent antibody. The nucleic acid moiety of the virus may be infective, and the protein component may provide immunologic specificity and serve to preserve transmissibility. The protein-deficient virus can be referred to as masked virus.

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