scholarly journals A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 451
Author(s):  
Ping Ouyang ◽  
Chenxin Fang ◽  
Jialun Han ◽  
Jingjing Zhang ◽  
Yuxing Yang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Gold Electrode ◽  
Folate Receptor ◽  
Protein Detection ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Target Protein ◽  
Receptor Protein ◽  
Electrochemical Dna Biosensor ◽  
Electrochemical Signal

The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA). We immobilized DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA. M1-Biotin DNA selectively combines with SA to generate M1-Biotin-SA DNA and protects M1-Biotin DNA from digestion by EXO III; therefore, M1-Biotin DNA remains intact on the electrode surface. M1-Biotin-SA DNA was modified with methylene blue (MB); the MB reporter molecule is located near the surface of the gold electrode, which generates a substantial electrochemical signal during the detection of SA. Through this strategy, we can exploit the presence or absence of an electrochemical signal to provide qualitative target protein determination as well as the strength of the electrochemical signal to quantitatively analyze the target protein concentration. This strategy has been proven to be used for the quantitative analysis of the interaction between biotin and streptavidin (SA). Under optimal conditions, the detection limit of the proposed biosensor is as low as 18.8 pM, and the linear range is from 0.5 nM to 5 μM, showing high sensitivity. The detection ability of this DNA biosensor in complex serum samples has also been studied. At the same time, we detected the folate receptor (FR) to confirm that this strategy can be used to detect other proteins. Therefore, this electrochemical DNA biosensor provides a sensitive, low-cost, and fast target protein detection platform, which may provide a reliable and powerful tool for early disease diagnosis.

Graphene Oxide/Perylene-Aniline Electrochemiluminescence Platform for Protein Detection Based on Molecule Recognition

2021 ◽  
Author(s):  
Peng Yin ◽  
Wei Zhang ◽  
Lei Shang ◽  
Rong-Na Ma ◽  
Liping Jia ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Folic Acid ◽  
Small Molecule ◽  
Folate Receptor ◽  
Protein Detection ◽  
Single Strand ◽  
Molecule Recognition

Most biosensors for protein folate receptor(FR) detection based on small molecule folic acid(FA) recognition usually introduced FA linked single strand DNA(FA-ssDNA) and nuclease to promote sensitivity, which increased expenses and...

A New Electrochemical Aptasensor for Protein Detection Based on Target Protein-Induced Strand Displacement

2013 ◽  
Vol 753-755 ◽  
pp. 2113-2116 ◽  
Author(s):  
Han Feng Cui ◽  
Hao Fan ◽  
Yan Lin
Keyword(s):  
Protein Detection ◽  
Thiol Group ◽  
Target Protein ◽  
Film Electrode ◽  
Electrochemical Aptasensor ◽  
Strand Displacement ◽  
Au Electrode ◽  
Mercury Film Electrode ◽  
Detection Probe ◽  
Electrochemical Signal

A sensitive electrochemical aptasensor for detection of thrombin based on target protein-induced strand displacement is presented. For this proposed aptasensor, dsDNA which is from immobilized probe DNA (IP) which has a hybridized with aptamer(Apt) initially was immobilized on the Au electrode through thiol group of IP，while a DNA labeled with PdS nanoparticles was used as a detection probe (DP-PdS). When Au electrode immersed into solution which contain target protein and DP-PdS, the aptamer preferred to form G-quarter structure with the present target protein and dsDNA was released into immobilized probe DNA (IP) which could be hybridized with DP-PdS. After dissolving PdS from electrode, a mercury-film electrode was used for electrochemical detection of these Pd2+ ions which offered excellent electrochemical signal transduction. Detection of thrombin was specific without being affected by the coexistence of other proteins such as BSA and lysozyme, and as low as 2.3×10-11 mol/L of thrombin was detected.

scholarly journals Colorimetric detection of protein via the terminal protection of small-molecule-linked DNA and unmodified gold nanoparticles

RSC Advances ◽  
2018 ◽  
Vol 8 (68) ◽  
pp. 38758-38764
Author(s):  
Jianwei Zhao ◽  
Cuiping Li ◽  
Guimin Ma ◽  
Wenhui Hao ◽  
Hongxia Jia
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Colorimetric Detection ◽  
Protein Detection ◽  
Target Protein

A novel colorimetric strategy for protein detection was developed based on unmodified gold nanoparticles (AuNPs) and terminal protection from a target protein.

Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode

2011 ◽  
Vol 16 (1) ◽  
pp. 273-282 ◽  
Author(s):  
Shafiquzzaman Siddiquee ◽  
Nor Azah Yusof ◽  
Abu Bakar Salleh ◽  
Soon Guan Tan ◽  
Fatimah Abu Bakar
Keyword(s):  
Ionic Liquid ◽  
Zno Nanoparticles ◽  
Trichoderma Harzianum ◽  
Gold Electrode ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor

Electrochemical DNA biosensor based on platinum-gold bimetal decorated graphene modified electrode for the detection of Vibrio Parahaemolyticus specific tlh gene sequence

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijun Yan ◽  
Fan Shi ◽  
Jingyao Zhang ◽  
Yanyan Niu ◽  
Lifang Huang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Modified Electrode ◽  
Vibrio Parahaemolyticus ◽  
Gene Sequence ◽  
Modified Electrodes ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: By using bimetal nanocomposite modified electrode, the electrochemical DNA biosensor showed the advantages of high sensitivity, low cost, rapid response and convenient operation, which was applied for disease diagnosis, food safety, and biological monitoring. Objective: A nanocomposite consisting of platinum (Pt)-gold (Au) bimetal and two-dimensional graphene (GR) was synthesized by hydrothermal method, which was modified on the surface of carbon ionic liquid electrode and further used for the immobilization of probe ssDNA related to Vibrio Parahaemolyticus tlh gene to construct an electrochemical DNA sensor. Method: Potassium ferricyanide was selected as electrochemical indicator, cyclic voltammetry was used to study the electrochemical behaviours of different modified electrodes and differential pulse voltammetry was employed to test the analytical performance of this biosensor for the detection of target gene sequence. Results: This electrochemical DNA biosensor could detect the Vibrio Parahaemolyticus tlh gene sequence as the linear concentration in the range from 1.0×10-13 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 2.91×10-14 mol L-1 (3σ). Conclusion: This proposed electrochemical DNA biosensor could be used to identify the special gene sequence with good selectivity, low detection limit and wide detection range.

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