scholarly journals Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 85 ◽  
Author(s):  
Brigitte Bruijns ◽  
Andrea Veciana ◽  
Roald Tiggelaar ◽  
Han Gardeniers
Keyword(s):  
Chemical Resistance ◽  
Multiple Displacement Amplification ◽  
Visible Region ◽  
Dna Amplification ◽  
Microfluidic Devices ◽  
Cyclic Olefin Copolymer ◽  
Color Test ◽  
Cyclic Olefin ◽  
On Chip ◽  
Amplification Reaction

Microfluidic devices offer important benefits for forensic applications, in particular for fast tests at a crime scene. A large portion of forensic applications require microfluidic chip material to show compatibility with biochemical reactions (such as amplification reactions), and to have high transparency in the visible region and high chemical resistance. Also, preferably, manufacturing should be simple. The characteristic properties of cyclic olefin copolymer (COC) fulfills these requirements and offers new opportunities for the development of new forensic tests. In this work, the versatility of COC as material for lab-on-a-chip (LOC) systems in forensic applications has been explored by realizing two proof-of-principle devices. Chemical resistance and optical transparency were investigated for the development of an on-chip presumptive color test to indicate the presence of an illicit substance through applying absorption spectroscopy. Furthermore, the compatibility of COC with a DNA amplification reaction was verified by performing an on-chip multiple displacement amplification (MDA) reaction.

Cyclic olefin copolymer as an X-ray compatible material for microfluidic devices

Lab on a Chip ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Manuela Denz ◽  
Gerrit Brehm ◽  
Clément Y. J. Hémonnot ◽  
Heidi Spears ◽  
Andrew Wittmeier ◽  
...  
Keyword(s):  
Small Angle ◽  
Microfluidic Devices ◽  
Hot Embossing ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

Fabrication of X-ray compatible microfluidic devices by hot embossing, and characterization for small angle X-ray scattering.

scholarly journals Fast Fabrication Process of Microfluidic Devices Based on Cyclic Olefin Copolymer

2014 ◽  
Vol 29 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Aymen Ben Azouz ◽  
Stephen Murphy ◽  
Shadi Karazi ◽  
Mercedes Vázquez ◽  
Dermot Brabazon
Keyword(s):  
Microfluidic Devices ◽  
Fabrication Process ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin

scholarly journals Cyclic Block Copolymer Microchannel Fabrication and Sealing for Microfluidics Applications

Inventions ◽  
2018 ◽  
Vol 3 (3) ◽  
pp. 49 ◽  
Author(s):  
Chia-Yi Yen ◽  
Moh-Ching Chang ◽  
Zong-Fu Shih ◽  
Yi-Hsing Lien ◽  
Chia-Wen Tsao
Keyword(s):  
Block Copolymer ◽  
Chemical Resistance ◽  
Microfluidic Devices ◽  
Cyclic Olefin ◽  
New Class ◽  
Front End ◽  
Cyclic Block ◽  
Cyclic Olefin Polymers ◽  
Polymer Microfabrication ◽  
Cyclic Olefin Copolymers

High mechanical rigidity, chemical resistance, and ultraviolet-visible light transmissivity of thermoplastics are attractive characteristics in microfluidics because various biomedical microfluidic devices require solvent, acid, or base manipulation, and optical observation or detection. The cyclic block copolymer (CBC) is a new class of thermoplastics with excellent optical properties, low water absorption, favorable chemical resistance, and low density, which make it ideal for use in polymer microfluidic applications. In the polymer microfabrication process, front-end microchannel fabrication and post-end bonding are critical steps that determine the success of polymer microfluidic devices. In this study, for the first time, we verified the performance of CBC created through front-end microchannel fabrication by applying hot embossing and post-end sealing and bonding, and using thermal fusion and ultraviolet (UV)/ozone surface-assist bonding methods. Two grades of CBC were evaluated and compared with two commonly used cyclic olefin polymers, cyclic olefin copolymers (COC), and cyclic olefin polymers (COP). The results indicated that CBCs provided favorable pattern transfer (>99%) efficiency and high bonding strength in microchannel fabrication and bonding procedures, which is ideal for use in microfluidics.

Genomic DNA amplification by the multiple displacement amplification (MDA) method

2009 ◽  
Vol 37 (2) ◽  
pp. 450-453 ◽  
Author(s):  
Roger S. Lasken
Keyword(s):  
Environmental Samples ◽  
Genomic Dna ◽  
Whole Genome Amplification ◽  
Multiple Displacement Amplification ◽  
Genomic Analysis ◽  
Dna Amplification ◽  
Genomic Sequencing ◽  
Extensive Coverage ◽  
Multiple Displacement Amplification Reaction ◽  
Amplification Reaction

Large amounts of DNA are frequently required for use in detection assays and genomic analysis. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. DNA amplification methods are often required to generate sufficient material from small specimens or environmental samples with low DNA content. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed.

scholarly journals A Microfluidic Approach for Biosensing DNA within Forensics

2020 ◽  
Vol 10 (20) ◽  
pp. 7067
Author(s):  
Brigitte Bruijns ◽  
Roald Tiggelaar ◽  
Han Gardeniers
Keyword(s):  
Dna Analysis ◽  
Multiple Displacement Amplification ◽  
Dna Amplification ◽  
Microfluidic Devices ◽  
Direct Analysis ◽  
Fast Analysis ◽  
Forensic Dna Analysis ◽  
Str Analysis ◽  
Promising Alternative ◽  
Experimental Findings

Reducing the risk of (cross-)contamination, improving the chain of custody, providing fast analysis times and options of direct analysis at crime scenes: these requirements within forensic DNA analysis can be met upon using microfluidic devices. To become generally applied in forensics, the most important requirements for microfluidic devices are: analysis time, method of DNA detection and biocompatibility of used materials. In this work an overview is provided about biosensing of DNA, by DNA profiling via standard short tandem repeat (STR) analysis or by next generation sequencing. The material of which a forensic microfluidic device is made is crucial: it should for example not inhibit DNA amplification and its thermal conductivity and optical transparency should be suitable for achieving fast analysis. The characteristics of three materials frequently used materials, i.e., glass, silicon and PDMS, are given, in addition to a promising alternative, viz. cyclic olefin copolymer (COC). New experimental findings are presented about the biocompatibility of COC and the use of COC chips for multiple displacement amplification and real-time monitoring of DNA amplification.

Rapid prototyping of plastic microfluidic devices in cyclic olefin copolymer (COC)

2005 ◽  
Author(s):  
Jin-Hwan Lee ◽  
Erik T. K. Peterson ◽  
Gabriel Dagani ◽  
Ian Papautsky
Keyword(s):  
Rapid Prototyping ◽  
Microfluidic Devices ◽  
Cyclic Olefin Copolymer ◽  
Cyclic Olefin
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