scholarly journals Immobilization of Camel Liver Catalase on Nanosilver-Coated Cotton Fabric

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 900
Author(s):  
Omar A. M. Al-Bar ◽  
Reda M. El-Shishtawy ◽  
Saleh A. Mohamed

Nanoparticles have the advantage of a superior surface area to volume ratio, and thus such materials are useful for enzyme immobilization. A silver nanoparticle coated cotton fabric (AgNp-CF) is used to immobilize camel liver catalase in the present work. The effect of loading levels of AgNp inside cotton fabrics on the immobilization of catalase was investigated. The results revealed that a 6 mL loading level of AgNp precursor (silver nitrate, 2 mM) at pH 8 showed the maximum immobilization efficiency (76%). The morphological properties of the cotton fabric (CF), AgNp-CF and AgNp-CF-catalase were characterized by SEM. The reusability of the immobilized enzyme was tested over ten reuses to show a 67% retained function of its initial activity. Compared with the soluble enzyme’s working pH (6.5), a rather broader working pH (6.5–7.0) was observed for the immobilized catalase. Additionally, the optimum working temperature increased from 30 for the soluble enzyme to 40 °C for the immobilized one, indicating thermal stability. The free and immobilized catalase enzyme’s Km values were 22.5 and 25 mM H2O2, respectively, reflecting the enzyme’s effective properties. The inhibitory effect of metal ions on the enzyme activity was higher toward soluble catalase than the immobilized catalase. This work has developed a method for immobilizing catalase to be useful for several applications.

Author(s):  
Cynthia Nagy ◽  
Robert Huszank ◽  
Attila Gaspar

AbstractThis paper aims at studying open channel geometries in a layer-bed-type immobilized enzyme reactor with computer-aided simulations. The main properties of these reactors are their simple channel pattern, simple immobilization procedure, regenerability, and disposability; all these features make these devices one of the simplest yet efficient enzymatic microreactors. The high surface-to-volume ratio of the reactor was achieved using narrow (25–75 μm wide) channels. The simulation demonstrated that curves support the mixing of solutions in the channel even in strong laminar flow conditions; thus, it is worth including several curves in the channel system. In the three different designs of microreactor proposed, the lengths of the channels were identical, but in two reactors, the liquid flow was split to 8 or 32 parallel streams at the inlet of the reactor. Despite their overall higher volumetric flow rate, the split-flow structures are advantageous due to the increased contact time. Saliva samples were used to test the efficiencies of the digestions in the microreactors. Graphical abstract


2018 ◽  
Vol 199 ◽  
pp. 390-396 ◽  
Author(s):  
Quan-Yong Cheng ◽  
Cheng-Shu Guan ◽  
Meng Wang ◽  
Yi-Dong Li ◽  
Jian-Bing Zeng

Cellulose ◽  
2018 ◽  
Vol 25 (12) ◽  
pp. 7393-7407 ◽  
Author(s):  
Mohamad M. Ayad ◽  
Wael A. Amer ◽  
Sawsan Zaghlol ◽  
Nela Maráková ◽  
Jaroslav Stejskal

Author(s):  
Anna Yunitsyna ◽  
Ernest Shtepani

Cities are a complex mass of morphological properties of many city fragments, which play a major role in energy consumption. Urban form, urban patterns, or city fragments can also be seen as defined by algorithms or form generators.  Cities are designed taking into account infrastructure, city standards and land use regulations. Energy efficiency of the urban form may be understood as the balance between gains and losses of energy, which may depend on a set of parameters mostly defined by the geometrical shape of the buildings and the distance between them. The study starts from the development and analysis of 60 hypothetical models in order to evaluate their energy efficiency potential. The Galapagos Evolutionary Solver is used as a tool in order to find the set of parameters, which brings to the morphological properties the optimal combination of density and surface-to-volume ratio. At the final stage morphological properties of 64 Prague’s patterns were selected.  Computer simulation and analysis is performed using the models extracted from the virtual Google Earth model of Prague. During the process of evaluation of the samples, the relationship between the urban form and such parameters as plot coverage, surface-to-volume ratio and the incident solar radiation was established and potentially higher energy efficient structures were indicated. As the result of analysis the interrelation between urban form and energy efficiency was established, which allowed to identify the urban patterns with the higher potential of energy efficiency.


2021 ◽  
Author(s):  
Xinhua Liu ◽  
Hailong Liu ◽  
Yinchun Fang

Abstract In this study, intumescent flame retardant coating of polyethylenimine/phytic acid (PEI/PA) with gradient structure was constructed on cotton fabric through facile layer-by-layer (LBL) assembly method. The LOI value of coated cotton fabric reached over 40% indicating excellent flame retardancy. Reasonable controlling the LBL assembly process of PEI/PA coating brought less influence to the physical properties of cotton fabrics. And the coated cotton fabric revealed good flame retardant washing durability. Thermogravimetric analysis results of coated cotton fabrics showed that PEI/PA flame retardant coating changed the thermal decomposition process and promoted char formation revealing the obviously condensed phase flame retardant action. SEM images of char residues revealed that PEI/PA flame retardant coating promoted to form the intumescent flame retardant (IFR) char layer showing obvious IFR action. This research provides novel strategy for the development of high-efficiency flame retardant cotton fabric with good durability and physical properties using simple LBL assembly method.


Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 928 ◽  
Author(s):  
Hyelim Kim ◽  
Sunhee Lee

We prepared a horseshoe-pattern type electrical heating textile that was coated with high graphene nanoplatelet (GNP) content (32 wt% to 64 wt%) of graphene nanoplatelet/poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) composite. Silver-coated conductive yarn is used as electrode in the sample to improve its flexibility and applicability as wearable textile. These graphene nanoplatelet/PVDF-HFP coated samples with various high-contents of graphene were characterized using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), sheet resistance analysis, and electrical heating performance analysis. Graphene nanoplatelet/PVDF-HFP coated cotton fabric improved the crystallinity and thermal stability with increasing thw high-content of GNP. With an increasing of the high-content of graphene nanoplatelet in the PVDF-HFP composite solution, the sheet resistance of samples tended to gradually decrease. That of, 64 wt% graphene nanoplatelet/PVDF-HFP composite coated sample (64 GR/cotton) was 44 Ω/sq. The electrical heating performance of graphene nanoplatelet/PVDF-HFP composite coated cotton fabric was improved with increasing the high-content of graphene nanoplatelet. When 5 V was applied to 64 GR/cotton, its surface temperature has been indicated to be about 48 °C and it could be used at a low voltage (<10 V). Thus, a horseshoe-pattern type electrical heating textile that is coated by high content of graphene nanoplatelet/PVDF-HFP composite solution sewn with silver-coated conductive yarn is expected to be applied to glove, shoes, jacket, and so on to improve its wearability and applicability.


RSC Advances ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 4180-4189 ◽  
Author(s):  
Yuzhou Li ◽  
Yufan Zhang ◽  
Haoran Zhang ◽  
Tie-ling Xing ◽  
Guo-qiang Chen

In recent years, the energy supply problem of wearable electronic equipment has become a topic of increasing concern. In this work, a solid, flexible and symmetrical supercapacitor based on graphene coated cotton fabric was fabricated.


2019 ◽  
Vol 58 (47) ◽  
pp. 21419-21430 ◽  
Author(s):  
Manoj Manickam ◽  
Prabunathan Pichaimani ◽  
Hariharan Arumugam ◽  
Alagar Muthukaruppan

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