scholarly journals Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-Like Cells

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3519
Author(s):  
Jiancheng Yang ◽  
Gejing Zhang ◽  
Qingmei Li ◽  
Qinghua Tang ◽  
Yan Feng ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Iron Metabolism ◽  
Biological Activities ◽  
Biological Effects ◽  
Iron Chelator ◽  
Cellular Viability ◽  
Cell Type ◽  
Static Magnetic Fields ◽  
Cellular Iron ◽  
Iron Preparation

There are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessary microelement that is involved in numerous life activities in cells. Studies have shown that high static magnetic fields (HiSMF) can regulate cellular iron metabolism. To illustrate the effect of HiSMF on activities of osteocytes, and whether iron is involved in this process, HiSMF of 16 tesla (T) was used, and the changes in cellular morphology, cytoskeleton, function-related protein expression, secretion of various cytokines, and iron metabolism in osteocytes under HiSMF were studied. In addition, the biological effects of HiSMF combined with iron preparation and iron chelator on osteocytes were also investigated. The results showed that HiSMF promoted cellular viability, decreased apoptosis, increased the fractal dimension of the cytoskeleton, altered the secretion of cytokines, and increased iron levels in osteocytes. Moreover, it was found that the biological effects of osteocytes under HiSMF are attenuated or enhanced by treatment with a certain concentration of iron. These data suggest that HiSMF-regulated cellular iron metabolism may be involved in altering the biological effects of osteocytes under HiSMF exposure.

The Cellular Labile Iron Pool and Intracellular Ferritin in K562 Cells

Blood ◽  
1999 ◽  
Vol 94 (6) ◽  
pp. 2128-2134 ◽  
Author(s):  
Abraham M. Konijn ◽  
Hava Glickstein ◽  
Boris Vaisman ◽  
Esther G. Meyron-Holtz ◽  
Itzchak N. Slotki ◽  
...  
Keyword(s):  
Biological Effects ◽  
Ferritin Level ◽  
Regulatory Protein ◽  
Iron Chelation ◽  
K562 Cells ◽  
Iron Chelator ◽  
Labile Iron Pool ◽  
Cellular Iron ◽  
Labile Iron ◽  
Iron Pool

Abstract The labile iron pool (LIP) harbors the metabolically active and regulatory forms of cellular iron. We assessed the role of intracellular ferritin in the maintenance of intracellular LIP levels. Treating K562 cells with the permeant chelator isonicotinoyl salicylaldehyde hydrazone reduced the LIP from 0.8 to 0.2 μmol/L, as monitored by the metalo-sensing probe calcein. When cells were reincubated in serum-free and chelator-free medium, the LIP partially recovered in a complex pattern. The first component of the LIP to reappear was relatively small and occurred within 1 hour, whereas the second was larger and relatively slow to occur, paralleling the decline in intracellular ferritin level (t½= 8 hours). Protease inhibitors such as leupeptin suppressed both the changes in ferritin levels and cellular LIP recovery after chelation. The changes in the LIP were also inversely reflected in the activity of iron regulatory protein (IRP). The 2 ferritin subunits, H and L, behaved qualitatively similarly in response to long-term treatments with the iron chelator deferoxamine, although L-ferritin declined more rapidly, resulting in a 4-fold higher H/L-ferritin ratio. The decline in L-ferritin, but not H-ferritin, was partially attenuated by the lysosomotrophic agent, chloroquine; on the other hand, antiproteases inhibited the degradation of both subunits to the same extent. These findings indicate that, after acute LIP depletion with fast-acting chelators, iron can be mobilized into the LIP from intracellular sources. The underlying mechanisms can be kinetically analyzed into components associated with fast release from accessible cellular sources and slow release from cytosolic ferritin via proteolysis. Because these iron forms are known to be redox-active, our studies are important for understanding the biological effects of cellular iron chelation.

scholarly journals Global transcriptome analysis reveals partial estrogen-like effects of karanjin in MCF-7 breast cancer cells

2021 ◽  
Author(s):  
Gaurav Bhatt ◽  
Akshita Gupta ◽  
Latha Rangan ◽  
Anil Mukund Limaye
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Target Genes ◽  
Biological Activities ◽  
Biological Effects ◽  
Dependent Manner ◽  
Cell Type ◽  
Concentration Dependent Manner ◽  
Mcf 7

Karanjin, an abundantly occurring furanoflavonoid in edible and non-edible legumes, exerts diverse biological effects in vivo, and in vitro. Its potential as an anticancer agent is also gaining traction following recent demonstrations of its anti-proliferative, cell cycle inhibitory, and pro-apoptotic effects. However, the universality of its anticancer potential is yet to be scrutinized, particularly so because flavonoids can act as selective estrogen receptor modulators (SERMs). Even the genomic correlates of its biological activities are yet to be examined in hormone responsive cells. This paper presents the early and direct transcriptomic footprint of 10 μM karanjin in MCF-7 breast cancer cells, using next generation sequencing technology (RNA-seq). We show that karanjin-modulated gene-expression repertoire is enriched in several hallmark gene sets, which include early estrogen-response, and G2/M checkpoint genes. Genes modulated by karanjin overlapped with those modulated by 1 nM 17β-estradiol (E2), or 1 μM tamoxifen. Karanjin altered the expression of selected estrogen-regulated genes in a cell-type, and concentration dependent manner. It downmodulated the expression of ERα protein in MCF-7 cells. Furthermore, ERα knockdown negatively impacted karanjins ability to modulate the expression of selected E2 target genes. Our data suggest that karanjin exerts its effects on ERα-positive breast cancer cells, at least in part, via ERα. The apparent SERM-like effects of karanjin pose a caveat to the anticancer potential of karanjin. In-depth studies on cell-type and concentration-dependent effects of karanjin may bring out its true potential in endocrine therapies.

Biological effects of static magnetic fields and zinc isotopes onE. colibacteria

2018 ◽  
Vol 40 (1) ◽  
pp. 62-73 ◽  
Author(s):  
Ulyana G. Letuta ◽  
Vitaly L. Berdinskiy
Keyword(s):  
Magnetic Fields ◽  
Biological Effects ◽  
Static Magnetic Fields ◽  
Zinc Isotopes

Model study of biological effects of weak static magnetic fields at the organismic and subcellular levels

2015 ◽  
Vol 461 (1) ◽  
pp. 116-119 ◽  
Author(s):  
V. E. Stefanov ◽  
B. F. Shchegolev ◽  
O. V. Kriyachko ◽  
N. V. Kuzmenko ◽  
S. V. Surma ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Biological Effects ◽  
Static Magnetic Fields ◽  
Model Study

scholarly journals Cell shape and plasma membrane alterations after static magnetic fields exposure

10.4081/840 ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 299 ◽  
Author(s):  
A Chionna ◽  
M Dwikat ◽  
E Panzarini ◽  
B Tenuzzo ◽  
EC Carlà ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Magnetic Fields ◽  
Cell Surface ◽  
Cell Shape ◽  
Biological Effects ◽  
Human Lymphocytes ◽  
Cell Types ◽  
Continuous Exposure ◽  
U937 Cells ◽  
Static Magnetic Fields

The biological effects of static magnetic fields (MFs) with intensity of 6 mT were investigated in lymphocytes and U937 cells in the presence or absence of apoptosis-inducing drugs by transmission (TEM) and scanning (SEM) electron microscopy. Lectin cytochemistry of ConA-FITC conjugates was used to analyze plasma membrane structural modifications. Static MFs modified cell shape, plasma membrane and increased the level of intracellular [Ca++] which plays an antiapoptotic role in both cell types. Modifications induced by the exposure to static MFs were irrespective of the presence or absence of apoptotic drugs or the cell type. Abundant lamellar-shaped microvilli were observed upon 24 hrs of continuous exposure to static MFs in contrast to the normally rough surface of U937 cells having numerous short microvilli. Conversely, lymphocytes lost their round shape and became irregularly elongated; lamellar shaped microvilli were found when cells were simultaneosly exposed to static MFs and apoptosis-inducing drugs. In our experiments, static MFs reduced the smoothness of the cell surface and partially impeded changes in distribution of cell surface glycans, both features being typical of apoptotic cells. Cell shape and plasma membrane structure modifications upon static MFs exposure were time-dependent. Lamellar microvilli were clearly observed before the distortion of cell shape, which was found at long times of exposure. MFs exposure promoted the rearrangement of F-actin filaments which, in turn, could be responsible for the cell surface modifications. Here we report data that support biological effects of static MFs on U937 cells and human lymphocytes. However, the involvement of these modifications in the onset of diseases needs to be further elucidated.

Biological effects of 6 mT static magnetic fields: A comparative study in different cell types

2006 ◽  
Vol 27 (7) ◽  
pp. 560-577 ◽  
Author(s):  
Bernadette Tenuzzo ◽  
Alfonsina Chionna ◽  
Elisa Panzarini ◽  
Remigio Lanubile ◽  
Patrizia Tarantino ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Comparative Study ◽  
Biological Effects ◽  
Cell Types ◽  
Static Magnetic Fields ◽  
Different Cell Types
Sign in / Sign up

Export Citation Format

Share Document