scholarly journals β-Aminobutyric Acid Priming Acquisition and Defense Response of Mango Fruit to Colletotrichum gloeosporioides Infection Based on Quantitative Proteomics

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1029 ◽  
Author(s):  
Taotao Li ◽  
Panhui Fan ◽  
Ze Yun ◽  
Guoxiang Jiang ◽  
Zhengke Zhang ◽  
...  
Keyword(s):  
Defense Response ◽  
Molecular Mechanisms ◽  
Colletotrichum Gloeosporioides ◽  
Lipid Transfer Protein ◽  
Sugar Metabolism ◽  
Aminobutyric Acid ◽  
Lipid Transfer ◽  
Post Translational Modification ◽  
Mango Fruit ◽  
Priming Phase

β-aminobutyric acid (BABA) is a new environmentally friendly agent to induce disease resistance by priming of defense in plants. However, molecular mechanisms underlying BABA-induced priming defense are not fully understood. Here, comprehensive analysis of priming mechanism of BABA-induced resistance was investigated based on mango-Colletotrichum gloeosporioides interaction system using iTRAQ-based proteome approach. Results showed that BABA treatments effectively inhibited the expansion of anthracnose caused by C. gleosporioides in mango fruit. Proteomic results revealed that stronger response to pathogen in BABA-primed mango fruit after C. gleosporioides inoculation might be attributed to differentially accumulated proteins involved in secondary metabolism, defense signaling and response, transcriptional regulation, protein post-translational modification, etc. Additionally, we testified the involvement of non-specific lipid-transfer protein (nsLTP) in the priming acquisition at early priming stage and memory in BABA-primed mango fruit. Meanwhile, spring effect was found in the primed mango fruit, indicated by inhibition of defense-related proteins at priming phase but stronger activation of defense response when exposure to pathogen compared with non-primed fruit. As an energy-saving strategy, BABA-induced priming might also alter sugar metabolism to provide more backbone for secondary metabolites biosynthesis. In sum, this study provided new clues to elucidate the mechanism of BABA-induced priming defense in harvested fruit.

scholarly journals Identification of Differentially Expressed Proteins in Sugarcane in Response to Infection by Xanthomonas albilineans Using iTRAQ Quantitative Proteomics

2020 ◽  
Vol 8 (1) ◽  
pp. 76
Author(s):  
Jian-Yu Meng ◽  
Mbuya Sylvain Ntambo ◽  
Philippe C. Rott ◽  
Hua-Ying Fu ◽  
Mei-Ting Huang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Vascular System ◽  
Lipid Transfer Protein ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Differentially Expressed Proteins ◽  
Lipid Transfer ◽  
Leaf Scald ◽  
Improvement Programs ◽  
Xanthomonas Albilineans

Sugarcane can suffer severe yield losses when affected by leaf scald, a disease caused by Xanthomonas albilineans. This bacterial pathogen colonizes the vascular system of sugarcane, which can result in reduced plant growth and plant death. In order to better understand the molecular mechanisms involved in the resistance of sugarcane to leaf scald, a comparative proteomic study was performed with two sugarcane cultivars inoculated with X. albilineans: one resistant (LCP 85-384) and one susceptible (ROC20) to leaf scald. The iTRAQ (isobaric tags for relative and absolute quantification) approach at 0 and 48 h post-inoculation (hpi) was used to identify and annotate differentially expressed proteins (DEPs). A total of 4295 proteins were associated with 1099 gene ontology (GO) terms by GO analysis. Among those, 285 were DEPs during X. albilineans infection in cultivars LCP 85-384 and ROC20. One hundred seventy-two DEPs were identified in resistant cultivar LCP 85-384, and 113 of these proteins were upregulated and 59 were downregulated. One hundred ninety-two DEPs were found in susceptible cultivar ROC20 and half of these (92) were upregulated, whereas the other half corresponded to downregulated proteins. The significantly upregulated DEPs in LCP 85-384 were involved in metabolic pathways, the biosynthesis of secondary metabolites, and the phenylpropanoid biosynthesis pathway. Additionally, the expression of seven candidate genes related to photosynthesis and glycolytic pathways, plant innate immune system, glycosylation process, plant cytochrome P450, and non-specific lipid transfer protein was verified based on transcription levels in sugarcane during infection by X. albilineans. Our findings shed new light on the differential expression of proteins in sugarcane cultivars in response to infection by X. albilineans. The identification of these genes provides important information for sugarcane variety improvement programs using molecular breeding strategies.

scholarly journals Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

2019 ◽  
Vol 39 (4) ◽  
Author(s):  
Álan C. Maracahipes ◽  
Gabriel B. Taveira ◽  
Erica O. Mello ◽  
André O. Carvalho ◽  
Rosana Rodrigues ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Colletotrichum Gloeosporioides ◽  
Protein Extraction ◽  
Lipid Transfer Protein ◽  
Extraction Process ◽  
Lipid Transfer ◽  
Protease Inhibition ◽  
Transfer Protein ◽  
Trypsin Inhibition

Abstract There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides. The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml−1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi ‘in vitro’. There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

β-Aminobutyric acid induces resistance of mango fruit to postharvest anthracnose caused by Colletotrichum gloeosporioides and enhances activity of fruit defense mechanisms

2013 ◽  
Vol 160 ◽  
pp. 78-84 ◽  
Author(s):  
Zhengke Zhang ◽  
Dongping Yang ◽  
Bo Yang ◽  
Zhaoyin Gao ◽  
Min Li ◽  
...  
Keyword(s):  
Defense Mechanisms ◽  
Colletotrichum Gloeosporioides ◽  
Aminobutyric Acid ◽  
Mango Fruit

scholarly journals Barley Lipid Transfer Protein, LTP1, Contains a New Type of Lipid-like Post-translational Modification*

2001 ◽  
Vol 276 (36) ◽  
pp. 33547-33553 ◽  
Author(s):  
Kresten Lindorff-Larsen ◽  
Mathilde H. Lerche ◽  
Flemming M. Poulsen ◽  
Peter Roepstorff ◽  
Jakob R. Winther
Keyword(s):  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Post Translational Modification ◽  
Transfer Protein ◽  
New Type

scholarly journals Identification of DIR1-dependant cellular responses required for guard cell systemic acquired resistance

2021 ◽  
Author(s):  
Lisa David ◽  
Jianing Kang ◽  
Joshua J Nicklay ◽  
Craig Dufresne ◽  
Sixue Chen
Keyword(s):  
Guard Cell ◽  
Molecular Mechanisms ◽  
Systemic Acquired Resistance ◽  
Lipid Transfer Protein ◽  
Acquired Resistance ◽  
Guard Cells ◽  
Lipid Transfer ◽  
Wild Type ◽  
Transfer Protein ◽  
Uninfected Plant

After localized invasion by bacterial pathogens, systemic acquired resistance (SAR) is induced in uninfected plant tissues, resulting in enhanced defense against a broad range of pathogens. Although SAR requires mobilization of signaling molecules via the plant vasculature, the specific molecular mechanisms remain elusive. The lipid transfer protein-defective in induced resistance 1-1 (DIR1-1) was identified in Arabidopsis thaliana by screening for mutants that were defective in SAR. Here we demonstrate that stomatal response to pathogens is altered in systemic leaves by SAR, and this guard cell SAR defense requires DIR1. Using a multi-omics approach, we have determined potential SAR signaling mechanisms specific for guard cells in systemic leaves by profiling metabolite, lipid, and protein differences between guard cells in wild type and dir1-1 mutant during SAR. We identified two 18C fatty acids and two 16C wax esters as putative SAR-related molecules dependent on DIR1. Proteins and metabolites related to amino acid biosynthesis and response to stimulus were also changed in guard cells of dir1-1 compared to wild type. Identification of guard cell-specific SAR-related molecules may lead to new avenues of genetic modification/molecular breeding for disease resistant plants.

scholarly journals Identification of DIR1-Dependant Cellular Responses in Guard Cell Systemic Acquired Resistance

2021 ◽  
Vol 8 ◽  
Author(s):  
Lisa David ◽  
Jianing Kang ◽  
Josh Nicklay ◽  
Craig Dufresne ◽  
Sixue Chen
Keyword(s):  
Guard Cell ◽  
Molecular Mechanisms ◽  
Systemic Acquired Resistance ◽  
Lipid Transfer Protein ◽  
Acquired Resistance ◽  
Guard Cells ◽  
Lipid Transfer ◽  
Wild Type ◽  
Transfer Protein ◽  
In The Wild

After localized invasion by bacterial pathogens, systemic acquired resistance (SAR) is induced in uninfected plant tissues, resulting in enhanced defense against a broad range of pathogens. Although SAR requires mobilization of signaling molecules via the plant vasculature, the specific molecular mechanisms remain elusive. The lipid transfer protein defective in induced resistance 1 (DIR1) was identified in Arabidopsis thaliana by screening for mutants that were defective in SAR. Here, we demonstrate that stomatal response to pathogens is altered in systemic leaves by SAR, and this guard cell SAR defense requires DIR1. Using a multi-omics approach, we have determined potential SAR signaling mechanisms specific for guard cells in systemic leaves by profiling metabolite, lipid, and protein differences between guard cells in the wild type and dir1-1 mutant during SAR. We identified two long-chain 18 C and 22 C fatty acids and two 16 C wax esters as putative SAR-related molecules dependent on DIR1. Proteins and metabolites related to amino acid biosynthesis and response to stimulus were also changed in guard cells of dir1-1 compared to the wild type. Identification of guard cell-specific SAR-related molecules may lead to new avenues of genetic modification/molecular breeding for disease-resistant plants.

scholarly journals Quantitative succinyl-proteome profiling of Chinese hickory (Carya cathayensis) during the grafting process

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Huwei Yuan ◽  
Juanjuan Chen ◽  
Ying Yang ◽  
Chenjia Shen ◽  
Dongbin Xu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Mechanisms ◽  
Carbon Fixation ◽  
Economic Value ◽  
Sugar Metabolism ◽  
Post Translational Modification ◽  
Affinity Enrichment ◽  
Lysine Succinylation ◽  
Starting Point ◽  
Carya Cathayensis

Abstract Background Chinese hickory (Carya cathayensis) is a popular nut plant having high economic value. Grafting is applied to accelerate the transition from vegetative phase to reproductive phase. Lysine succinylation occurs frequently in the proteins associated with metabolic pathways, which may participate in the regulation of the grafting process. However, the exact regulatory mechanism underlying grafting process in Chinese hickory has not been studied at post-translational modification level. Results A comprehensive proteome-wide lysine succinylation profiling of Chinese hickory was explored by a newly developed method combining affinity enrichment and high-resolution LC-MS/MS. In total, 259 succinylation sites in 202 proteins were identified, representing the first comprehensive lysine succinylome in Chinese hickory. The succinylation was biased to occur in the cytosolic proteins of Chinese hickory. Moreover, four conserved succinylation motifs were identified in the succinylated peptides. Comparison of two grafting stages of Chinese hickory revealed that the differential expressed succinylated proteins were mainly involved in sugar metabolism, carbon fixation, amino acid metabolism and plant-pathogen interaction. Besides, seven heat shock proteins (HSPs) with 11 succinylation sites were also identified, all of which were observed to be up-regulated during the grafting process. Conclusions Succinylation of the proteins involved in amino acid biosynthesis might be required for a successful grafting. Succinylated HSPs might play a role in stress tolerance of the grafted Chinese hickory plants. Our results can be a good resource for functional validation of the succinylated proteins and a starting point for the investigation of molecular mechanisms during lysine succinylation occurring at grafting site.

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