scholarly journals Skin Improvement Effects of Gardeniae fructus Extract in HaCaT Keratinocytes, B16F10 Melanocytes, and CCD-986sk Fibroblast Cells

Cosmetics ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 48 ◽  
Author(s):  
Se Hyang Hong ◽  
Jin Mo Ku ◽  
Seung Hwan Lee ◽  
Ho Jong Shim ◽  
Dong Sun Park ◽  
...  
Keyword(s):  
Free Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Fibroblast Cells ◽  
Mrna Levels ◽  
Protein Levels ◽  
Free Radical Scavenging Assay ◽  
Free Radical Activity ◽  
Dose Dependent ◽  
Whitening Activity

The development of functional cosmetics with skin improvement effects from natural sources is necessary. In this study, the antioxidant, antiwrinkling, moisturizing, and whitening effects of Gardeniae fructus extract (GF) were investigated in keratinocytes, melanocytes, and fibroblast cells. Antioxidant activity was determined by a DPPH free radical scavenging assay. MMP-1, MMP-9, HAS1, and filaggrin mRNA levels were measured by RT-PCR in keratinocytes and fibroblast cells. MITF and tyrosinase protein levels were evaluated by blotting analysis in melanocytes. DPPH free radical activity was investigated to determine whether GF showed dose-dependent inhibitory activity. GF induced the upregulation of HAS1 and filaggrin mRNA expression in keratinocytes and fibroblast cells. GF led to the downregulation of MMP mRNA levels in keratinocytes and fibroblast cells. Western blotting was performed to confirm the whitening-related protein (MITF and tyrosinase) levels induced by GF in melanocytes, and the inhibitory activity was superior to that of the α-MSH used for the comparison test. GF showed marked antioxidant, antiwrinkling, skin moisturizing, and whitening activity in keratinocytes, melanocytes, and fibroblast cells. Through the results of these experiments, the applicability of GF as a natural and functional cosmetic material was verified.

Effects of a Flavonoid Extract from Cynomorium songaricum on the Swimming Endurance of Rats

2010 ◽  
Vol 38 (01) ◽  
pp. 65-73 ◽  
Author(s):  
Fa-Rong Yu ◽  
Ying Liu ◽  
Yong-Zhi Cui ◽  
Er-Qing Chan ◽  
Ming-Ren Xie ◽  
...  
Keyword(s):  
Body Weight ◽  
Free Radical ◽  
Radical Scavenging ◽  
Copper Zinc Superoxide Dismutase ◽  
Zinc Superoxide Dismutase ◽  
Body Weights ◽  
Copper Zinc ◽  
Scavenging Enzymes ◽  
Swimming Endurance ◽  
Dose Dependent

The present study investigated the effects of a flavonoid extract from Cynomorium songaricum on the swimming endurance of rats by measuring changes of free radical scavenging enzymes, such as CuZn-SOD (copper, zinc-superoxide dismutase) and GSH-px (glutathione peroxidase), and body weights. Significant and dose-dependent antioxidant and anti-fatigue effects of flavonoids (rutin, catechin and isoquercitrin) on swimming rats were observed during 10 days of swimming exercise. After treatment with the flavonoid extract at doses of 0.5, 1.0, and 2.0 g/kg body weight, the CuZn-SOD and GSH-px activities in swimming rats were increased by 1.4%, 3.3%, 4.1% and 112.2%, 208.7%, 261.7%, respectively, while the levels of MDA (malondialdehyde) were decreased by 64.7%, 79.4%, and 86.4% respectively. Furthermore, the average body weight and the total swimming time were increased by 3.1%, 8.8%, 10.6%, and 7.7%, 34.5%, 61.5%, respectively. Our experimental results suggest that flavonoid supplementation could not only reduce free radical formation and scavenge free radicals, but also enhance endurance exercise performance by reducing muscle fatigue.

scholarly journals Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay

Data in Brief ◽  
2018 ◽  
Vol 17 ◽  
pp. 870-875 ◽  
Author(s):  
Kotaro Hara ◽  
Takao Someya ◽  
Katsura Sano ◽  
Yoshimasa Sagane ◽  
Toshihiro Watanabe ◽  
...  
Keyword(s):  
Free Radical ◽  
Sri Lanka ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Abstract 492: Arachidonic Acid Induces Activation of Platelet PF4 and Par-1 mRNA, Which Is Attenuated by Aspirin

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Liang Hu ◽  
Michael A Nardi ◽  
Michael Merolla ◽  
Yajaira Suarez ◽  
Jeffrey Berger
Keyword(s):  
Arachidonic Acid ◽  
Platelet Activation ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Thiazole Orange ◽  
Platelet Activity ◽  
Protein Levels ◽  
Reticulated Platelets ◽  
Mrna And Protein Expression ◽  
Dose Dependent

Arachidonic acid (AA) is converted to thromboxane A2 via the cyclooxygenase pathway; however its exact mechanism of platelet activation is uncertain. Inhibition of this pathway via aspirin highlights the importance of this pathway in decreasing thrombotic events. In the present study, we investigate the effect of AA on platelet activity indicators (leukocyte- and monocyte-platelet aggregation [LPA, MPA] and reticulated platelets [RP]), as well as the expression (mRNA and protein) of platelet markers PF4 and Par-1, previously well established platelet transcripts with quantitative determinations. To this end, whole blood was incubated with AA (150mM) for 30 min at room temperature in the absence or presence of aspirin (1mM) prior to addition of antibodies for platelet activity indicators, and isolating platelets for mRNA and protein expression. LPA and MPA were significantly increased after AA stimulation in a dose dependent manner, and were inhibited by aspirin treatment. AA significantly increased PF4 and Par-1 protein level as determined by flow cytometry and western blot assays. Pretreatment with aspirin also attenuated this increase in protein levels. Surprisingly, AA stimulation significantly increased thiazole orange staining (a measure of nucleic acids), another marker of increased platelet activity. Importantly, these results suggest that AA-mediated platelet activation produced an overall increase in platelet total RNA content. To confirm these findings, we analyzed the mRNA expression of PF4 and Par-1 by quantitative real time PCR from platelets treated with AA. Interestingly, AA significantly up-regulated the platelet mRNA transcripts of PF4 and Par-1 by 40% to 60%, and pretreatment with aspirin completely attenuated this effect supporting the specificity of the AA effect on platelet RNA. Altogether, these data suggest that platelet mRNA is affected by AA stimulation, which is attenuated by pretreatment with aspirin. However, the mechanisms responsible for the increased mRNA levels and expression of PF4 and Par-1 (processing of pre-RNA to mRNA) require further investigation. Importantly, our findings provide novel insight regarding platelet activation and a better understanding of mediators in the processes of thrombosis and hemostasis.

In Vitro Regeneration and Free Radical Scavenging Assay of Hypericum perforatum L.

2018 ◽  
Vol 42 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Mohammad Yaseen Mir ◽  
Azra N. Kamili ◽  
Qazi P. Hassan ◽  
Sabreena Rafi ◽  
Javid A. Parray ◽  
...  
Keyword(s):  
Free Radical ◽  
Hypericum Perforatum ◽  
In Vitro Regeneration ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypericum Perforatum L ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Study on Scavenging Free Radical Activity with Polysaccharides Materials in Chuanxiong Based on Composite Properties of Biomaterials

2012 ◽  
Vol 583 ◽  
pp. 244-247
Author(s):  
Yong Guang Bi ◽  
Chun Chun Liu
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Superoxide Anion Radical ◽  
Dose Effect ◽  
Spectrophotometric Detection ◽  
Free Radical Activity ◽  
Composite Properties

By using spectrophotometric detection of the Chuanxiong polysaccharides on the free radical scavenging. The results show that the Chuanxiong polysaccharides on DPPH radical, hydroxyl radical (HO•) and superoxide anion radical (•) clearance. Scavenging ability with the the Chuanxiong polysaccharide concentration increased, and showed the dose-effect relationship. Concentration of 2.0mg/mL when DPPH radicals clear the rate of 35.61%, while the rate of hydroxyl radicals and superoxide anion radical scavenging, clear the rate of 57.78% and 57.14%. Chuanxiong polysaccharide is an ideal natural antioxidants and good prospects for the development of biomedical composites.

scholarly journals Study on α-glucosidase enzyme inhibitory activity and DPPH free radical scavenging of green coffee bean extract (Coffea canephora)

2019 ◽  
Vol 35 (2) ◽  
Author(s):  
Dang Kim Thu ◽  
Vu Manh Hung ◽  
Nguyen Thi Trang ◽  
Bui Thanh Tung
Keyword(s):  
Free Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Coffea Canephora ◽  
Coffee Bean ◽  
Green Coffee ◽  
Coffee Beans ◽  
Etoac Fraction ◽  
Treatment Of Diabetes ◽  
Green Coffee Bean Extract

Abstract: α-glucosidase enzyme is one of the important molecular targets in the treatment of diabetes. In addition, free radicals are the cause of insulin resistance, damage β- cell pancreatic, glucose uptake disorders and induced diabetes. In this study we evaluated the inhibitory effect of α-glucosidase enzyme and antioxidant effect by using DPPH free radical scavenging method of green coffee bean extract (Coffea canephora) and its fractions. Coffee beans were pulverized, extracted with ethanol 70% by sonications,  and fractionated with n-hexane, ethyl acetate (EtOAc) and n-butanol (n-BuOH) solvents. Our results showed that coffee bean extract has a strong α-glucosidase enzyme inhibitory activity, especially EtOAc fraction with an IC50 value of 2.21 ± 0.04 µg/mL. Furthermore, the coffee bean extract has an antioxidant effect by DPPH radical scavenging ability, and EtOAc fraction has the highest effect with an IC50 value of 25.69 ± 3.08 µg/ml. Our  results show that green coffee beans and EtOAc fraction have potential effect in preventing and supporting for the treatment of diabetes. Keywords Coffee; Coffea canephora; free radical; α-glucosidase; DPPH. References [1] Federation ID. IDF Diabetes Atlas 8th Edition (2017).[2] Wright Jr E, Scism‐Bacon J, Glass L. Oxidative stress in type 2 diabetes: the role of fasting and postprandial glycaemia. International journal of clinical practice 60(3) (2006) 308.[3] X. Chen. A review on coffee leaves: Phytochemicals, bioactivities and applications. Critical reviews in food science and nutrition 59(6) (2019) 1008.[4] Chu Y-F. Coffee: emerging health effects and disease prevention. John Wiley & Sons (2012).[5] N.T. Hai, D.K. Thu, B.T. Tung. Sarcandra glabra Extract Protects against Scopolamine Induced Cognitive Deficits by Modulating Neuroinflammation and the Cholinergic System. Current Enzyme Inhibition 14(3) (2018) 210.[6] B.T. Tung, D.K. Thu, P.T. Hai, N.T. Hai. Evaluation of α-glucosidase inhibitory activity of fractions from Punica granatum Linn fruits (in Vietnamese), Journal of Traditional Vietnamese Medicine and Pharmacy 5(18) (2018) 59.[7] S. Lenzen. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia 51(2) (2008) 216.[8] K. Shapiro, W.C. Gong . Natural products used for diabetes. Journal of the American Pharmaceutical Association 42(2) (2002) 217.[9] O. Babova, A. Occhipinti, M.E. Maffei. Chemical partitioning and antioxidant capacity of green coffee (Coffea arabica and Coffea canephora) of different geographical origin. Phytochemistry 123 (2016) 33.[10] A. Priftis, D. Stagos, K. Konstantinopoulos, C. Tsitsimpikou, D.A. Spandidos, A.M. Tsatsakis, et al. Comparison of antioxidant activity between green and roasted coffee beans using molecular methods. Molecular medicine reports 12(5) (2015) 7293.[11] N. Liang, D.D. Kitts. Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules 19(11) (2014) 19180.[12] Vieira TMFdS. Potential antioxidant of brazilian coffee from the region of Cerrado. Food Science and Technology 38(3) (2018) 447.[13] S.D. Kim. α-Glucosidase inhibitor isolated from coffee. J Microbiol Biotechnol 25(2) (2015) 174.[14] Y. Zheng, K. Liu, G. Jia, H. Li, L. Han, Y. Kimura Effect of hot-water extract of coffee seeds on postprandial blood glucose concentration in rats. (2007).    

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