Addressing Human Skin Ethnicity: Contribution of Tissue Engineering to the Development of Cosmetic Ingredients

Recent publications describe various skin disorders in relation to phototypes and aging. The highest phototypes (III to VI) are more sensitive to acne, with the appearance of dark spots due to the inflammation induced by Cutibacterium acnes (previously Propionibacterium acnes). Dryness with aging is due to a lower activity of specific enzymes involved in the maturation of lipids in the stratum corneum. To observe and understand these cutaneous issues, tissue engineering is a perfect tool. Since several years, pigmented epidermis with melanocytes derived from specific phototypes allow to develop in vitro models for biological investigations. In the present study, several models were developed to study various skin disorders associated with phototypes and aging. These models were also used to evaluate selected ingredients’ ability to decrease the negative effects of acne, inflammation, and cutaneous dryness. Hyperpigmentation was observed on our reconstructed pigmented epidermis after the application of C. acnes, and pollutant (PM10) application induced increased inflammatory cytokine release. Tissue engineering and molecular biology offer the capability to modify genetically cells to decrease the expression of targeted proteins. In our case, GCase was silenced to decrease the maturation of lipids and in turn modify the epidermal barrier function. These in vitro models assisted in the development of ethnic skin-focused cosmetic ingredients.

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Hydrogels for Liver Tissue Engineering

Bioengineering ◽

10.3390/bioengineering6030059 ◽

2019 ◽

Vol 6 (3) ◽

pp. 59 ◽

Cited By ~ 11

Author(s):

Shicheng Ye ◽

Jochem W.B. Boeter ◽

Louis C. Penning ◽

Bart Spee ◽

Kerstin Schneeberger

Keyword(s):

Tissue Engineering ◽

Liver Tissue ◽

Drug Testing ◽

In Vitro Models ◽

Liver Tissue Engineering ◽

Synthetic Materials ◽

Toxicological Studies ◽

End Stage

Bioengineered livers are promising in vitro models for drug testing, toxicological studies, and as disease models, and might in the future be an alternative for donor organs to treat end-stage liver diseases. Liver tissue engineering (LTE) aims to construct liver models that are physiologically relevant. To make bioengineered livers, the two most important ingredients are hepatic cells and supportive materials such as hydrogels. In the past decades, dozens of hydrogels have been developed to act as supportive materials, and some have been used for in vitro models and formed functional liver constructs. However, currently none of the used hydrogels are suitable for in vivo transplantation. Here, the histology of the human liver and its relationship with LTE is introduced. After that, significant characteristics of hydrogels are described focusing on LTE. Then, both natural and synthetic materials utilized in hydrogels for LTE are reviewed individually. Finally, a conclusion is drawn on a comparison of the different hydrogels and their characteristics and ideal hydrogels are proposed to promote LTE.

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Calcium sulphate mixed with antibiotics does not decrease efficacy against Cutibacterium acnes (formerly Propionibacterium acnes), in vitro study

Journal of Orthopaedics ◽

10.1016/j.jor.2019.11.028 ◽

2020 ◽

Vol 19 ◽

pp. 138-142

Author(s):

Anne Couture ◽

Valéry Lavergne ◽

Emilie Sandman ◽

Jean-Michel Leduc ◽

Benoit Benoit ◽

...

Keyword(s):

Propionibacterium Acnes ◽

In Vitro Study ◽

Calcium Sulphate ◽

Vitro Study ◽

Cutibacterium Acnes

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3-D Cell Culture Systems in Bone Marrow Tissue and Organoid Engineering, and BM Phantoms as In Vitro Models of Hematological Cancer Therapeutics—A Review

Materials ◽

10.3390/ma13245609 ◽

2020 ◽

Vol 13 (24) ◽

pp. 5609

Author(s):

Dasharatham Janagama ◽

Susanta K. Hui

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Large Scale ◽

Cancer Therapeutics ◽

In Vitro Models ◽

Cancer Tissue ◽

Hematological Cancer ◽

Culture Systems ◽

Hematological Cancers

We review the state-of-the-art in bone and marrow tissue engineering (BMTE) and hematological cancer tissue engineering (HCTE) in light of the recent interest in bone marrow environment and pathophysiology of hematological cancers. This review focuses on engineered BM tissue and organoids as in vitro models of hematological cancer therapeutics, along with identification of BM components and their integration as synthetically engineered BM mimetic scaffolds. In addition, the review details interaction dynamics of various BM and hematologic cancer (HC) cell types in co-culture systems of engineered BM tissues/phantoms as well as their relation to drug resistance and cytotoxicity. Interaction between hematological cancer cells and their niche, and the difference with respect to the healthy niche microenvironment narrated. Future perspectives of BMTE for in vitro disease models, BM regeneration and large scale ex vivo expansion of hematopoietic and mesenchymal stem cells for transplantation and therapy are explained. We conclude by overviewing the clinical application of biomaterials in BM and HC pathophysiology and its challenges and opportunities.

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Organ Culture System for Mechanobiology Studies of the Intervertebral Disc

Advances in Bioengineering ◽

10.1115/imece2004-61248 ◽

2004 ◽

Author(s):

Cynthia R. Lee ◽

Mauro Alini ◽

James C. Iatridis

Keyword(s):

Tissue Engineering ◽

Intervertebral Disc ◽

Culture System ◽

Static Load ◽

In Vitro Models ◽

Disc Regeneration ◽

Organ Culture System ◽

In Vitro Culture System ◽

Vertebral Endplates

The development of in vitro models is critical for furthering understanding of the intervertebral disc and the development of disc regeneration/tissue engineering. An in vitro culture system targeted towards mechano-biology studies of the intervertebral disc (IVD) was built and validated using bovine coccygeal discs. Discs were maintained in culture for up to one week with and without vertebral endplates. Water content and glycosaminoglycan content were found to be stable and cells were metabolically active when cultured under a 5kg static load.

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Dynamic in vitro models for tumor tissue engineering

Cancer Letters ◽

10.1016/j.canlet.2019.01.043 ◽

2019 ◽

Vol 449 ◽

pp. 178-185 ◽

Cited By ~ 1

Author(s):

Daniel Karami ◽

Nathan Richbourg ◽

Vassilios Sikavitsas

Keyword(s):

Tissue Engineering ◽

Tumor Tissue ◽

In Vitro Models

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Hollow Fiber and Nanofiber Membranes in Bioartificial Liver and Neuronal Tissue Engineering

Cells Tissues Organs ◽

10.1159/000511680 ◽

2021 ◽

pp. 1-30

Author(s):

Sabrina Morelli ◽

Antonella Piscioneri ◽

Simona Salerno ◽

Loredana De Bartolo

Keyword(s):

Tissue Engineering ◽

Drug Testing ◽

Electrospun Fiber ◽

In Vitro Models ◽

Bioartificial Liver ◽

Neuronal Tissue ◽

Biological Phenomena ◽

The Creation ◽

And Control

To date, the creation of biomimetic devices for the regeneration and repair of injured or diseased tissues and organs remains a crucial challenge in tissue engineering. Membrane technology offers advanced approaches to realize multifunctional tools with permissive environments well-controlled at molecular level for the development of functional tissues and organs. Membranes in fiber configuration with precisely controlled, tunable topography, and physical, biochemical, and mechanical cues, can direct and control the function of different kinds of cells toward the recovery from disorders and injuries. At the same time, fiber tools also provide the potential to model diseases in vitro for investigating specific biological phenomena as well as for drug testing. The purpose of this review is to present an overview of the literature concerning the development of hollow fibers and electrospun fiber membranes used in bioartificial organs, tissue engineered constructs, and in vitro bioreactors. With the aim to highlight the main biomedical applications of fiber-based systems, the first part reviews the fibers for bioartificial liver and liver tissue engineering with special attention to their multifunctional role in the long-term maintenance of specific liver functions and in driving hepatocyte differentiation. The second part reports the fiber-based systems used for neuronal tissue applications including advanced approaches for the creation of novel nerve conduits and in vitro models of brain tissue. Besides presenting recent advances and achievements, this work also delineates existing limitations and highlights emerging possibilities and future prospects in this field.

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Application of Graphene in Tissue Engineering of the Nervous System

International Journal of Molecular Sciences ◽

10.3390/ijms23010033 ◽

2021 ◽

Vol 23 (1) ◽

pp. 33

Author(s):

Karolina Ławkowska ◽

Marta Pokrywczyńska ◽

Krzysztof Koper ◽

Luis Alex Kluth ◽

Tomasz Drewa ◽

...

Keyword(s):

Tissue Engineering ◽

Nervous System ◽

Antibacterial Effect ◽

Transplant Rejection ◽

Organ Donor ◽

High Strength ◽

Immunosuppressive Drugs ◽

Negative Effects

Graphene is the thinnest two-dimensional (2D), only one carbon atom thick, but one of the strongest biomaterials. Due to its unique structure, it has many unique properties used in tissue engineering of the nervous system, such as high strength, flexibility, adequate softness, electrical conductivity, antibacterial effect, and the ability to penetrate the blood–brain barrier (BBB). Graphene is also characterized by the possibility of modifications that allow for even wider application and adaptation to cell cultures of specific cells and tissues, both in vitro and in vivo. Moreover, by using the patient's own cells for cell culture, it will be possible to produce tissues and organs that can be re-transplanted without transplant rejection, the negative effects of taking immunosuppressive drugs, and waiting for an appropriate organ donor.

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