aMMP-8 Point-of-Care/Chairside Oral Fluid Technology as a Rapid, Non-Invasive Tool for Periodontitis and Peri-Implantitis Screening in a Medical Care Setting

This communication article addresses currently available rapid non-invasive methods to screen and detect periodontitis and dental peri-implantitis. In this regard, oral fluid biomarkers have been researched extensively but self-reported oral health (SROH)-questionnaires have also been developed. Both alternatives may offer a quick and easy way to screen and detect diseased patients. Active matrix metalloproteinase (aMMP-8) is one of the most validated biomarkers for screening and detecting periodontal breakdown related to periodontitis and peri-implantitis and monitoring their treatment effects revealing successful, less- and non-successful treatment results. Currently available aMMP-8 lateral-flow technologies allow this kind of analysis, as demonstrated here, to be conducted quantitatively online and real-time as point-of-care/chairside testing in dental and even medical care settings. In this study, an aMMP-8 peri-implant sulcular fluid point-of-care-test diagnosed peri-implantitis and healthy implants far more accurately than bleeding-on-probing or the other biomarkers, such as polymorphonuclear (PMN)/neutrophil elastase, myeloperoxidase and MMP-9. Although, SROH-questionnaires allow screening in similar settings but they lack the information about the current disease activity of periodontitis and peri-implantitis, which is of essential value in periodontal diagnostics and treatment monitoring. Thus, both methods can be considered as adjunct methods for periodontitis and peri-implant diagnostics, but the value of oral fluid biomarkers analysis does not seem to be substitutable.

Download Full-text

P.10.10 FEASIBILITY OF HCV INFECTION SCREENING IN BABY BOOMERS WITH A RAPID, NON-INVASIVE POINT-OF-CARE TEST IN ORAL FLUID

Digestive and Liver Disease ◽

10.1016/s1590-8658(16)30274-2 ◽

2016 ◽

Vol 48 ◽

pp. e181

Author(s):

J.F. Blume ◽

L. Simone ◽

S. Boccia ◽

L. Trevisani ◽

P. Pazzi

Keyword(s):

Baby Boomers ◽

Oral Fluid ◽

Point Of Care ◽

Hcv Infection ◽

Non Invasive ◽

Point Of Care Test

Download Full-text

A rapid, non-invasive tool for periodontitis screening in a medical care setting

BMC Oral Health ◽

10.1186/s12903-019-0784-7 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 8

Author(s):

Martijn J. L. Verhulst ◽

Wijnand J. Teeuw ◽

Sergio Bizzarro ◽

Joris Muris ◽

Naichuan Su ◽

...

Keyword(s):

Medical Care ◽

Care Setting ◽

Non Invasive

Download Full-text

Active MMP-8 (aMMP-8) as a Grading and Staging Biomarker in the Periodontitis Classification

Diagnostics ◽

10.3390/diagnostics10020061 ◽

2020 ◽

Vol 10 (2) ◽

pp. 61 ◽

Cited By ~ 16

Author(s):

Timo Sorsa ◽

Saeed Alassiri ◽

Andreas Grigoriadis ◽

Ismo T. Räisänen ◽

Pirjo Pärnänen ◽

...

Keyword(s):

Classification System ◽

Point Of Care ◽

Bleeding On Probing ◽

Active Matrix ◽

Spearman's Rho ◽

Periodontal Breakdown ◽

Severe Periodontitis ◽

Spearman’S Rho ◽

Bonferroni Test ◽

Post Hoc

The aim of this study was to investigate the utility of incorporating active matrix metalloproteinase-8 (aMMP-8) as a biomarker into the new periodontitis classification system (stage/grade) presented in 2018. This study included 150 Greek adults aged 25–78, of whom 74 were men and 76 women. Participants were tested with an aMMP-8 point-of-care mouthrinse test, after which a full-mouth clinical examination was performed to assess their periodontal and oral health. The aMMP-8 levels in mouthrinse were significantly lower among healthy patients compared with patients in more severe periodontitis stages and grades (Kruskal–Wallis test and Dunn–Bonferroni test for pairwise post-hoc comparisons; p < 0.01 and p < 0.05, respectively). Furthermore, aMMP-8 levels were less correlated with plaque levels than bleeding on probing (BOP) (Spearman’s rho = 0.269, p < 0.001; Spearman’s rho = 0.586, p < 0.001); respectively). Thus, aMMP-8 was more robust to the confounding effects of oral hygiene than traditional periodontal parameter bleeding on probing. The aMMP-8 point-of-care mouthrinse test can be utilized as an adjunctive and preventive diagnostic tool to identify periodontal disease, classified by stage and grade, and ongoing periodontal breakdown chairside in clinical practice in only 5 min. Overall, integrating aMMP-8 into the new periodontitis classification system seems beneficial.

Download Full-text

Evaluation of a Rapid Oral Fluid Point-of-Care Test for MDMA

Journal of Analytical Toxicology ◽

10.1093/jat/31.2.98 ◽

2007 ◽

Vol 31 (2) ◽

pp. 98-104 ◽

Cited By ~ 9

Author(s):

L. Wilson ◽

A. Jehanli ◽

C. Hand ◽

G. Cooper ◽

R. Smith

Keyword(s):

Oral Fluid ◽

Point Of Care ◽

Point Of Care Test

Download Full-text

Utility of B-Natriuretic Peptide As a Rapid, Point-of-Care Test for Screening Patients Undergoing Echocardiography to Determine Left Ventricular Dysfunction. Utility of B-Type Natriuretic Peptide in the Diagnosis of Congestive Heart Failure in an Urgent-Care Setting

Congestive Heart Failure ◽

10.1111/j.1527-5299.2001.00275.x ◽

2001 ◽

Vol 7 (6) ◽

pp. 329-330

Keyword(s):

Heart Failure ◽

Congestive Heart Failure ◽

Natriuretic Peptide ◽

Care Setting ◽

Ventricular Dysfunction ◽

Point Of Care ◽

Left Ventricular ◽

Urgent Care ◽

Point Of Care Test ◽

Urgent Care Setting

Download Full-text

Host-Modulation Therapy and Chair-Side Diagnostics in the Treatment of Peri-Implantitis

Biosensors ◽

10.3390/bios10050044 ◽

2020 ◽

Vol 10 (5) ◽

pp. 44 ◽

Cited By ~ 3

Author(s):

Timo Sorsa ◽

Joseph Bacigalupo ◽

Mauno Könönen ◽

Pirjo Pärnänen ◽

Ismo T. Räisänen

Keyword(s):

Point Of Care ◽

Low Cost ◽

Diagnostic Tools ◽

Low Grade ◽

Active Matrix ◽

Point Of Care Test ◽

Increased Risk ◽

Prevention And Treatment ◽

Potential Benefits ◽

Low Grade Inflammation

Previous studies report periodontitis and peri-implantitis being able to induce systemic low-grade inflammation, which is known to be associated with increased risk for some systemic medical disease such as cardiovascular disease. In this regard, recent studies have shown that host modulation therapy (HMT) together with traditional mechanical and surgical treatment not only cease the progression of periodontitis but also reduce the systemic collagenolytic biomarkers in both oral fluids and circulation. This suggests that the corresponding adjunctive HMT-medication could be effective in the prevention and treatment of dental peri-implantitis, as well. Furthermore, low-cost, safe, and practical oral fluid active matrix metalloproteinase-8 (aMMP-8) lateral-flow immunotests have been proposed as point-of-care/chair-side diagnostic tools to detect peri-implantitis and periodontitis, and to monitor their effective resolutions, while using various therapeutic strategies, including host modulation. This study reports the potential benefits of HMT-medication in the prevention and treatment of dental peri-implantitis among five patients (four of five were current/ex-smokers). In addition, the aMMP-8 point-of-care test diagnosed 20 peri-implantitis and 20 healthy controls correctly. In conclusion, this study and previous studies support the potential effectiveness of HMT-medication(s) and point-of-care/chair-side technologies in the treatment and diagnostics/monitoring of peri-implantitis. However, more studies are needed to further confirm this.

Download Full-text