scholarly journals The Diagnostic Performance of Interleukin-6 and C-Reactive Protein for Early Identification of Neonatal Sepsis

Diagnostics ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 978
Author(s):  
Belay Tessema ◽  
Norman Lippmann ◽  
Anja Willenberg ◽  
Matthias Knüpfer ◽  
Ulrich Sack ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Neonatal Sepsis ◽  
Diagnostic Performance ◽  
University Hospital ◽  
C Reactive Protein ◽  
Clinical Sepsis ◽  
Reactive Protein ◽  
Increased Sensitivity ◽  
And Performance ◽  
Culture Negative

Interleukin-6 (IL-6) and C-reactive protein (CRP) are being used for diagnosis of sepsis. However, studies have reported varying cut-off levels and diagnostic performance. This study aims to investigate the optimal cut-off levels and performance of IL-6 and CRP for the diagnosis of neonatal sepsis. The study was conducted at the University Hospital of Leipzig, Germany from November 2012 to June 2020. A total of 899 neonates: 104 culture proven sepsis, 160 clinical sepsis, and 625 controls were included. Blood culture was performed using BacT/ALERT 3D system. IL-6 and CRP were analyzed by electrochemiluminescent immunoassay and immunoturbidimetric assay, respectively. Data were analyzed using SPSS 20 statistical software. Among neonates with proven sepsis, the optimal cut-off value of IL-6 was 313.5 pg/mL. The optimal cut-off values for CRP in 5 days serial measurements (CRP1, CRP2, CRP3, CRP4, and CRP5) were 2.15 mg/L, 8.01 mg/L, 6.80 mg/L, 5.25 mg/L, and 3.72 mg/L, respectively. IL-6 showed 73.1% sensitivity, 80.2% specificity, 37.6% PPV, and 94.8% NPV. The highest performance of CRP was observed in the second day with 89.4% sensitivity, 97.3% specificity, 94.5% PPV, and 98.3% NPV. The combination of IL-6 and CRP showed increase in sensitivity with decrease in specificity. In conclusion, this study defines the optimal cut-off values for IL-6 and CRP. The combination of IL-6 and CRP demonstrated increased sensitivity. The CRP 2 at cut-off 8.01 mg/L showed the highest diagnostic performance for identification of culture negative clinical sepsis cases. We recommend the combination of IL-6 (≥313.5 pg/mL) and CRP1 (≥2.15 mg/L) or IL-6 (≥313.5 pg/mL) and CRP2 (≥8.01 mg/L) for early and accurate diagnosis of neonatal sepsis. The recommendation is based on increased sensitivity, that is, to minimize the risk of any missing cases of sepsis. The CRP2 alone at cut-off 8.01 mg/L might be used to identify clinical sepsis cases among culture negative sepsis suspected neonates in hospital settings.

Efficacy of new leukocyte parameters versus serum C-reactive protein, procalcitonin, and interleukin-6 in the diagnosis of neonatal sepsis

2016 ◽  
Vol 58 (2) ◽  
pp. 119-125 ◽  
Author(s):  
H. Tolga Çelik ◽  
Oytun Portakal ◽  
Şule Yiğit ◽  
Gülşen Hasçelik ◽  
Ayşe Korkmaz ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Neonatal Sepsis ◽  
C Reactive Protein ◽  
Reactive Protein

ASSESSMENT OF INTERLEUKIN 6 AND HIGH SENSITIVE C-REACTIVE PROTEIN AS EARLY MARKER OF NEONATAL SEPSIS

2020 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
maram el-shafee ◽  
atef noseir ◽  
Amal abdellatif ◽  
nagla khalefa
Keyword(s):  
Interleukin 6 ◽  
Neonatal Sepsis ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Early Marker

Diagnostic value of resistin and visfatin, in comparison with C-reactive protein, procalcitonin and interleukin-6 in neonatal sepsis

2011 ◽  
Vol 22 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Ferhat Cekmez ◽  
Fuat Emre Canpolat ◽  
Merih Çetinkaya ◽  
Seçil Aydinöz ◽  
Gokhan Aydemir ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Neonatal Sepsis ◽  
Diagnostic Value ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals DIAGNOSTIC VALUE OF SIMULTANEOUS MEASUREMENT OF PROCALCITONIN, INTERLEUKIN-6 AND HS CRP IN PREDICTION OF EARLY-ONSET NEONATAL SEPSIS

2012 ◽  
Vol 4 (1) ◽  
pp. e2012028 ◽  
Author(s):  
Alireza Abdollahi ◽  
Saeed Shoar ◽  
Fatemeh Nayyeri ◽  
Mamak Shariat
Keyword(s):  
Interleukin 6 ◽  
Simultaneous Measurement ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Clinical Signs ◽  
C Reactive Protein ◽  
Maternal Risk ◽  
Reactive Protein ◽  
Early Onset Sepsis ◽  
Hs Crp

Neonatal sepsis is a major cause of morbidities and mortalities mostly remarkable in the third world nations .We aimed to assess the value of simultaneous measurement of procalcitonin (PCT) and interleukin-6 (IL-6) in association with high sensitive- C reactive protein in prediction of early neonatal sepsis.We performed a follow- up study on 95 neonates who were below 12 hours (h) of age, had clinical signs of sepsis or maternal risk factors for sepsis. Neonates were assigned to 4 groups including “proven early-onset sepsis”, “clinical early-onset sepsis”, “negative infectious status”, and “uncertain infectious status”. Blood samples were obtained within the first 12 h of birth repeated between 24 hours and 36 hours of age for determination of serum levels of PCT, IL-6, high sensitivie- C Reactive Protein (hs-CRP), and white blood cell (WBC) count.On admission, neonates with sepsis had a higher WBC count, IL-6, PCT, and hs-CRP levels compared with those neonates without sepsis. This remained significant even after 12-24 hours of admission. Also, patients with clinical evidences of sepsis had a higher serum level of PCT and IL-6 within 12-24 hours after admission compared to the patients with uncertain sepsis. In final The combination of IL-6, hs-CRP, and PCT seems to be predictive in diagnosis of early onset neonatal sepsis.

Diagnostic Markers for Neonatal Sepsis: Comparing C-reactive Protein, Interleukin-6 and Immunoglobulin M

2007 ◽  
Vol 65 (2) ◽  
pp. 171-175 ◽  
Author(s):  
M. Khassawneh ◽  
W. A. Hayajneh ◽  
H. Kofahi ◽  
Y. Khader ◽  
Z. Amarin ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Neonatal Sepsis ◽  
Diagnostic Markers ◽  
Immunoglobulin M ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Qualitative C-reactive protein as a marker of neonatal sepsis in a tertiary neonatal unit in Sudan

2013 ◽  
Vol 1 (2) ◽  
pp. 21
Author(s):  
Abdelmoneim E. Kheir ◽  
Ghada A. Jobara ◽  
Kamal M. Elhag ◽  
Mohamed Z. Karar
Keyword(s):  
Risk Factors ◽  
Blood Culture ◽  
Neonatal Sepsis ◽  
Neonatal Intensive Care ◽  
University Hospital ◽  
C Reactive Protein ◽  
Chi Square ◽  
Reactive Protein ◽  
Chi Square Test ◽  
A Prospective Study

Sepsis is one of the most common causes of morbidity and mortality in newborns. Diagnosis of neonatal sepsis may be difficult because clinical presentations are often non-specific. The aim of this study was to evaluate the role of qualitative C-reactive protein in the diagnosis of neonatal sepsis, and examine the correlation between C-reactive protein, blood culture and risk factors for sepsis. This was a prospective study, conducted in the Neonatal Intensive Care Unit at Soba University Hospital, Sudan. A total of seventy babies with a clinical diagnosis of sepsis were included. Chi square test was used to determine the association between C-reactive protein and risk factors for sepsis and also the association between C-reactive protein and blood culture. Blood culture was positive in 41.4% of babies, and C-reactive protein was positive in 58% of babies with positive blood culture. There was significant association between C-reactive protein results and blood culture (P=0.00). In conclusion, we can assume that Creactive protein is a reliable diagnostic marker of neonatal sepsis, especially in developing communities with poor resources.

scholarly journals 632. Genetic Diversity of Carbapenem-Resistant Klebsiella pneumoniae Causing Late-Onset Neonatal Sepsis in Intensive Care Unit of Caro University Hospital, Cairo, Egypt

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S293-S293
Author(s):  
Doaa Ghaith ◽  
Halaa Mufeed ◽  
Sherif Elanwary ◽  
Mai Zafer ◽  
Mohamed Alagamy ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Neonatal Sepsis ◽  
Gel Electrophoresis ◽  
Genetic Relatedness ◽  
Late Onset ◽  
Pulsed Field Gel Electrophoresis ◽  
University Hospital ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Carbapenem Resistant

Abstract Background Neonatal sepsis poses a great challenge for clinicians and infection control practitioners. Carbapenem-resistant Klebsiella pneumoniae (CRKP) is rapidly increasing and poses a major threat to neonates. Our research aim was to examine the phenotypes, genotypes, and genetic relatedness of CRKP in late-onset neonatal sepsis in the neonatal intensive care unit (NICU) at Cairo University Hospital. Methods Our study included 88 neonates diagnosed with sepsis; 58 with late-onset sepsis (LOS) and 30 with early-onset sepsis (EOS) admitted to the NICU between November 2015 and April 2016. Laboratory investigations included (vomplete blood count, C-reactive protein, serum interleukin-6 level by ELISA technique and blood culture). bacterial identification and antibiotic susceptibility testing were done by automated VITEK 2 compact system (BioMérieux, France). Detection of carbapenemases (OXA-48, NDM, IMP, KPC, and VIM) by multiplex PCR and pulsed-field gel electrophoresis (PFGE). Results K. pneumoniae was the most common encountered pathogen in the LOS group (37.9%) with a mean sepsis score of 6.39 when compared with the 33 EOS group (P < 0.005). The interleukin ratio, C-reactive protein, and interleukin-6 levels were significantly high in the K. group (P ˂ 0.001). The most prevalent 35 carbapenemase gene in the NICU, OXA-48, was identified in 14/23 (60.8%) 36 isolates followed by NDM-1 in 12/23 (52.2%) isolates as detected by multiplex 37 PCR. Coexistence of both carbapenemases was found in 52.2% (12/23). By investigating the genetic relatedness of CRKP by pulsed-field gel electrophoresis, 23 isolates of K. pneumoniae revealed various PFGE patterns, demonstrating that 40 the isolates were nonclonal. Conclusion In conclusion, carbapenam-resistant Klebsiella pneumoniae remains the most frequent organism detected in neonatal sepsis. Our results revealed that CRKP isolates were not clonal. Extra data are required on the rates of birth asphyxia and microbiology of neonatal infection since reduced records of diseases sets gaps in understanding how to improve existing practices. Infection control actions including antibiotic stewardship programs with continuous surveillance to trace emerging CRKP infections in the early hours as possible particularly in units at risk as NICUs. Disclosures All authors: No reported disclosures.

Sign in / Sign up

Export Citation Format

Share Document