scholarly journals A Preliminary Investigation on Human Tears by Means of Surface Enhanced Raman Spectroscopy

Proceedings ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 18
Author(s):  
Carlo Camerlingo ◽  
Mikhail Lisitskiy ◽  
Maria Lepore ◽  
Marianna Portaccio ◽  
Daniela Montorio ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Capillary Tube ◽  
Preliminary Investigation ◽  
Surface Enhanced Raman Spectroscopy ◽  
Nano Particles ◽  
Glass Substrates ◽  
Non Invasive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Inflammatory Processes

A preliminary investigation on human tears by Surface Enhanced Raman Spectroscopy (SERS) is reported. This study is aimed to test the feasibility of SERS for sensing tear composition and potential changes in their composition due to pathologies, eye diseases and/or inflammatory processes. Home-made gold nano-particles have been used for preparing glass substrates that are able to efficiently induce a SERS effect in fluid samples excited by HeNe laser (λ = 633 nm). The system has been preliminarily tested on Rhodamine 6G aqueous solutions at different concentrations, proving the possibility to sense substance concentration as low as a few μM, i.e., of the order of the main tear organic component levels. Tears have been obtained by informed healthy patients by means of non-invasive methods based on a direct collection of the tear fluid by means of a suitable micro-capillary tube. A clear SERS response has also been obtained for human tear samples, allowing a preliminary insight on tear composition.

Non-invasive diagnosis of Crohn's disease based on SERS combined with PCA-SVM

2021 ◽  
Author(s):  
Bingyan Li ◽  
Yaling Wu ◽  
Zijie Wang ◽  
Mengmeng Xing ◽  
Weimin Xu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Surface Enhanced Raman Spectroscopy ◽  
Metabolic Change ◽  
Healthy Controls ◽  
Non Invasive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

The metabolic change of patients (Crohn's disease (CD)/active CD (aCD)/inactive CD (iCD)) and healthy controls (HC) could be identified by measuring urine with surface-enhanced Raman spectroscopy (SERS).

scholarly journals Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1177 ◽  
Author(s):  
Carlo Camerlingo ◽  
Mikhail Lisitskiy ◽  
Maria Lepore ◽  
Marianna Portaccio ◽  
Daniela Montorio ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Whole Body ◽  
Sources Of Information ◽  
Glass Substrates ◽  
Organic Components ◽  
Interesting Insight ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Unique Method

Tears are exceptionally rich sources of information on the health status of the eyes, as well as of whole body functionality, due to the presence of a large variety of salts and organic components whose concentration can be altered by pathologies, eye diseases and/or inflammatory processes. Surface enhanced Raman spectroscopy (SERS) provides a unique method for analyzing low concentrations of organic fluids such as tears. In this work, a home-made colloid of gold nanoparticles has been used for preparing glass substrates able to efficiently induce an SERS effect in fluid samples excited by a He–Ne laser ( λ = 633 nm). The method has been preliminary tested on Rhodamine 6G aqueous solutions at different concentrations, proving the possibility to sense substance concentrations as low as few μ M, i.e., of the order of the main tear organic components. A clear SERS response has been obtained for human tear samples, allowing an interesting insight into tear composition. In particular, aspartic acid and glutamic acid have been shown to be possible markers for two important human tear components, i.e., lactoferrin and lysozyme.

Sign in / Sign up

Export Citation Format

Share Document