scholarly journals Monitoring System for Laboratory Mice Transportation: A Novel Concept for the Measurement of Physiological and Environmental Parameters

Electronics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 34 ◽  
Author(s):  
Carlos González-Sánchez ◽  
Juan-Carlos Fraile ◽  
Javier Pérez-Turiel ◽  
Ellen Damm ◽  
Jochen G. Schneider ◽  
...  
Keyword(s):  
Heart Rate ◽  
Biomedical Research ◽  
Electric Potential ◽  
Integrated Circuit ◽  
Laboratory Animal ◽  
Environmental Parameters ◽  
Breathing Rate ◽  
Laboratory Mice ◽  
Novel Concept ◽  
Transportation Container

Laboratory mice are used in biomedical research as “models” for studying human disease. These mice may be subject to significant levels of stress during transportation that can cause alterations that could negatively affect the results of the performed investigation. Here, we present the design and realization of a prototypical transportation container for laboratory mice, which may contribute to improved laboratory animal welfare. This prototype incorporates electric potential integrated circuit (EPIC) sensors, which have been shown to allow the recording of physiological parameters (heart rate and breathing rate) and other sensors for recording environmental parameters during mouse transportation. This allows for the estimation of the stress levels suffered by mice. First experimental results for capturing physiological and environmental parameters are shown and discussed.

Changes in Blood Pressure, Heart Rate and Breathing Rate During Moderate Swimming Activity in Rainbow Trout

1967 ◽  
Vol 46 (2) ◽  
pp. 307-315 ◽  
Author(s):  
E. DON STEVENS ◽  
D. J. RANDALL
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Rainbow Trout ◽  
Venous Blood ◽  
The Body ◽  
Swimming Activity ◽  
Breathing Rate ◽  
Blood Pressures ◽  
Ventral Aorta ◽  
Pressure Changes

1. Changes in blood pressure in the dorsal aorta, ventral aorta and subintestinal vein, as well as changes in heart rate and breathing rate during moderate swimming activity in the rainbow trout are reported. 2. Blood pressures both afferent and efferent to the gills increased during swimming and then returned to normal levels within 30 min. after exercise. 3. Venous blood pressure was characterized by periodic increases during swimming. The pressure changes were not in phase with the body movements. 4. Although total venous return to the heart increased during swimming, a decreased blood flow was recorded in the subintestinal vein. 5. Heart rate and breathing rate increased during swimming and then decreased when swimming ceased. 6. Some possible mechanisms regulating heart and breathing rates are discussed.

INFLUENCE OF THE AGE ON BAROREFLEX SYNCHRONIZATION OF ARTERIAL PRESSURE AND HEART RATE DURING THE PASSIVE STANDING TEST

2021 ◽  
Vol 55 (1) ◽  
pp. 46-50
Author(s):  
Е.А. Orlova ◽  
◽  
О.S. Tarasova ◽  
V.D. Son'kin ◽  
А.S. Borovik ◽  
...  
Keyword(s):  
Heart Rate ◽  
Phase Synchronization ◽  
Tilt Test ◽  
Breathing Rate ◽  
Age Related ◽  
Head Up Tilt ◽  
Wave Range ◽  
Standing Test ◽  
Head Up Tilt Test ◽  
Over 40 Years

Age-related changes in phase synchronization of spontaneous blood pressure (BP) and heart rate (HR) fluctuations within the baroreflex wave range (about 0.1 Hz) were studied in 66 subjects aged 20 to 52 years. Measurements performed during the head-up tilt test included continuous BP monitoring using the volume-compensation method, ECG recording for ensuing HR calculation, and breathing rate recording. The phase synchronization index (PSI) was used for evaluation of BP-HR coupling. In supine position, phase synchronization in the subjects over 40 years was higher as compared with their counterparts at the age of 20 to 24 years. Along with that, less pronounced PSI increase in people over 40 in response to the tilt test suggests a decline of the baroreflex activity with aging.

scholarly journals One score to rule them all: severity assessment in laboratory mice

2020 ◽  
Author(s):  
Steven R. Talbot ◽  
Birgitta Struve ◽  
Laura Wassermann ◽  
Miriam Heider ◽  
Nora Weegh ◽  
...  
Keyword(s):  
Heart Rate ◽  
Animal Welfare ◽  
Biomedical Research ◽  
Restraint Stress ◽  
Assessment Tool ◽  
Postoperative Recovery ◽  
Assessment System ◽  
Severity Assessment ◽  
Severity Levels ◽  
Individual Variables

AbstractAnimal welfare and the refinement of experimental procedures are fundamental aspects of biomedical research. They provide the basis for robust experimental designs and reproducibility of results. In many countries, the determination of welfare is a mandatory legal requirement and implies the assessment of the degree of the severity that an animal experiences during an experiment. However, for an effective severity assessment, an objective and exact approach/system/strategy is needed. In light of these demands, we have developed the Relative Severity Assessment (RELSA) score.This comprehensive composite score was established on the basis of physiological and behavioral data from a surgical mouse study. Body weight, the Mouse Grimace Scale score, burrowing behavior, and the telemetry-derived parameters heart rate, heart rate variability, temperature, and general activity were used to investigate the quality of indicating severity during postoperative recovery. The RELSA scores not only revealed individual severity levels but also allowed a comparison of severity in distinct mouse models addressing colitis, sepsis, and restraint stress using a k-means clustering approach with the maximum achieved RELSA scores.We discriminated and classified data from sepsis nonsurvivors into the highest relative severity level. Data from mice after intraperitoneal transmitter implantation and sepsis survivor al were located in the next lower cluster, while data from mice subjected to colitis and restraint stress were placed in the lowest severity cluster. Analysis of individual variables and their combinations revealed model- and time-dependent contributions to severity levels.In conclusion, we propose the RELSA score as a validated tool for objective real-time applicability in severity assessment and as a first step towards a unified and accessible risk assessment tool in biomedical research. As an effective severity assessment system, it will fundamentally improve animal welfare, as well as data quality and reproducibility.

scholarly journals Effect of Intrahepatic Arterial Delivery of Sorafenib on Normal Liver Tissue of Rabbit: An Experimental Study

2020 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Eerdunbagena Ning ◽  
Zhijun Wang
Keyword(s):  
Heart Rate ◽  
Rectal Temperature ◽  
Liver Tissue ◽  
High Performance ◽  
Arterial Embolization ◽  
Hepatic Arterial Infusion ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
Breathing Rate ◽  
Local Necrosis

Objective: To assess the safety, feasibility and eluting efficiency of intrahepatic arterial delivery of sorafenib on normal liver tissue of rabbit. Methods: 24 New Zealand rabbits were randomly divided into three groups: group Ⅰ (Lipiodol-sorafenid), group Ⅱ (Lipiodol) and group Ⅲ (Sorafenib). Group Ⅰ and Ⅱ were treated by transcatheter selective hepatic arterial embolization with emulsions of lipiodol and sorafenib or with only lipiodol, while group Ⅲ was given hepatic arterial infusion with sorafenib. Sorafenib concentration in plasma was determined by HPLC (high performance liquid chromatography) in 0 min, 20 min, 1h, 2h, 4h, 8h, 16h, 32h and 48h respectively. The breathing rate, heart rate, rectal temperature and body weight were measured, as well the blood routine test and the function of liver, kidney, and heart. Two animals of each group were respectively killed in the 3rd day, 1st, 3rd and 6th week after treatment. Histopathologic study was done to liver, heart, kinney, lung, brain, gall bladder and intestine. Result: ① The peak sorafenib concentration (Cmax)and AUC(Area under curve) in plasma in groupⅠwas 2.46±0.101μg/ml and 945.72 ± 52.3 μg/mL.min respectively, while in group Ⅲ which was 3.78±0.180 ug/ml and 546.98±21.1μg/mL.min. Compared with groupⅢ, the Cmax and AUC of groupⅠhad a significant statistics difference (p<0.05). ② The breathing rate, heart rate, rectal temperature and AST/ALT,WBC,NEU% of group Ⅰand groupⅢhas a significant statistics difference(p<0.05) in the 3rd day. ③CK ,CK-MB, DB, Cr，BUN,RBC,PLT in plasma did not change in all group.④Local necrosis was seen in group Ⅰand group Ⅱin the 3rd day and 1st week, but they did not seem to be different. Group Ⅲ showed no necrosis. Granulation tissue with bile duty, portal vein and microfossils hyperplasia were seen in local necrosis area in the 3rd week. No pathological changes were found in brain, heart, kidney, intestine and gallbladder. Conclusion: TAE with emulsions of lipiodol and sorafenib is feasible, safe and has some slow-release effect.

scholarly journals The Physiological Effects of IPAD Digital Games on Children with Autism

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Nur Zakiah Mohd Saat ◽  
Najwa Suhaili Md Zin ◽  
Sazlina Kamaralzaman
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Children With Autism ◽  
Cross Sectional Study ◽  
Breathing Rate ◽  
Blood Pressure Monitor ◽  
Pressure Monitor ◽  
Cross Sectional ◽  
Strategy Games ◽  
The Mean

Background: Previous studies found that the use of an iPad® in intervention programmes benefited the skills of daily living of children with autism. The purpose of the study was to determine the physiological changes in children while playing action and strategy games on an iPad. Methodology: A cross-sectional study was conducted using Powerlab 24/6T, a blood pressure monitor, respiratory belt, and finger pulse transducer. The children were asked to play two types of game. The duration for each game was 10 minutes. The measurement of blood pressure - both systolic and diastolic, heart rate and breathing rate were taken at three consecutive times (before, during and after each game). The inclusion criteria are children aged 7 to 12 years old who have been diagnosed with a mild type of autism (Pervasive developmental disorder). The exclusion criteria were children who had co-morbidities such as epilepsy, migraine, heart, and lung problems, depression, physical disabilities and visual impairments.  Results: Nineteen children with autism participated in this study. The result indicated that the mean systolic and diastolic blood pressures increased before and after playing strategy games and action games. However, compared to pre-play values, the mean heart rate and breathing rate decreased both during and after playing both types of game. Meanwhile, there was significant mean difference for breathing rate for both types of game (p<0.05). Conclusion: Playing strategy and action games did not affect the measured physiological parameters of children with autism. 

scholarly journals Unstressing intemperate models: how cold stress undermines mouse modeling

2012 ◽  
Vol 209 (6) ◽  
pp. 1069-1074 ◽  
Author(s):  
Christopher L. Karp
Keyword(s):  
Cold Stress ◽  
Biomedical Research ◽  
Current Model ◽  
Environmental Variable ◽  
Model System ◽  
Laboratory Mice ◽  
Therapeutic Approaches ◽  
Mechanistic Analysis ◽  
Mouse Modeling

Mus musculus enjoys pride of place at the center of contemporary biomedical research. Despite being the current model system of choice for in vivo mechanistic analysis, mice have clear limitations. The literature is littered with examples of therapeutic approaches that showed promise in mouse models but failed in clinical trials. More generally, mice often provide poor mimics of the human diseases being modeled. Available data suggest that the cold stress to which laboratory mice are ubiquitously subjected profoundly affects mouse physiology in ways that impair the modeling of human homeostasis and disease. Experimental attention to this key, albeit largely ignored, environmental variable is likely to have a broad transformative effect on biomedical research.

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