Three-Dimensional Reconstruction of Fleece Fabric Surface for Thickness Evaluation

Aiming at solving the problem of manually measuring the fabric surface thickness, this paper proposes a three-dimensional (3D) reconstruction method based on the tangential two-dimensional (2D) sequence images. Firstly, the characteristic region of the fabric surface is extracted. Secondly, the image is splitting based on the maximum between-class variance method. Thirdly, the splitting image is processed by the morphological method. Fourthly, the canny operator is used to obtain the edge detection for calculating the edge contour coordinate. Finally, the surf function is used to reconstruct the 3D model of the fabric surface. To evaluate the performance of the proposed 3D model, the thickness and relief degree of the fabric surface are used, and the comparison between the proposed method and the manual measurement is carried out. The results demonstrate that, under a reasonable relief degree condition, the proposed method is more effective to evaluate the thickness of the fabric surface and the estimated thickness is more accurate than the manually measured one.

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Computer-Aided Three-Dimensional Reconstruction: A Method of Measuring Temporal Bone Structures Including the Length of the Cochlea

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348948909800705 ◽

1989 ◽

Vol 98 (7) ◽

pp. 515-522 ◽

Cited By ~ 68

Author(s):

Akira Takagi ◽

Isamu Sando

Keyword(s):

Temporal Bone ◽

Three Dimensional ◽

Computer Software ◽

Three Dimensions ◽

Reconstruction Method ◽

Two Dimensional ◽

Three Dimensional Reconstruction ◽

Computer Aided ◽

Dimensional Reconstruction ◽

Bone Structures

To visualize the three-dimensional (3-D) shapes of structures in the temporal bone from histologic sections, we developed computer software to create computer-generated 3-D images from two-dimensional (2-D) materials. Using those images, we have been able to measure these structures in three dimensions. This means that quantitative 3-D measurements can be performed regardless of the angle at which the specimen was cut. This report describes this method for 3-D reconstruction and measurement and discusses its implications. For instance, in addition to showing the 3-D shape of the cochlea from different angles, we have been able to measure the length of the cochlea and have shown that measurements made by use of the conventional 2-D graphic reconstruction method can be distorted considerably by variations in cutting angle of the specimen.

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THREE-DIMENSIONAL RECONSTRUCTION OF OBJECTS IN A DYNAMIC SCENE ON VIDEO STREAM

Podvodnye issledovaniia i robototehnika ◽

10.37102/24094609.2020.33.3.002 ◽

2020 ◽

Author(s):

В.А. Бобков ◽

А.П. Кудряшов

Keyword(s):

3D Model ◽

Three Dimensional ◽

Video Stream ◽

Reconstruction Method ◽

Three Dimensional Reconstruction ◽

Dynamic Scene ◽

Structure And Motion ◽

Dimensional Reconstruction ◽

Point Representation ◽

Dynamic Objects

Представлен метод 3D реконструкции объектов в динамической сцене в рамках общего подхода к решению проблемы восстановления структуры и движения по изображениям. Подход в целом основывается на точечном представлении объектов и применении метода визуальной одометрии для расчета движения динамических объектов по стереовидеопотоку. На этапе подготовки входных данных для метода реконструкции выполняются идентификация динамических объектов и вычисление траекторий их движения. Согласно предлагаемому методу 3D модель динамического объекта строится посредством объединения его видов, получаемых из разных позиций камеры. Проведенные эксперименты с виртуальными сценами подтвердили работоспособность предложенных алгоритмов. The article presents the method of three-dimensional reconstruction of objects on a dynamic scene in the framework of a common approach to solving the problem of restoring the structure and motion by images. In general, the proposed approach is based on point representation of the object and utilization of the visual odometry method to calculate the motion of dynamic objects by stereo video stream. The stage of preparation of input data for the reconstruction method consists of the identification of dynamic objects and calculating trajectories of its motion. According to the proposed method, the 3D model of a dynamic object is reconstructed by combining its views received from different camera positions. Conducted experimental research with virtual scenes proved the performance capabilities of proposed algorithms.

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Computer Assisted Image Reconstruction of Oligomer Structure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092323 ◽

1976 ◽

Vol 34 ◽

pp. 472-473

Author(s):

A.M. Jones ◽

A. Max Fiskin

Keyword(s):

Three Dimensional ◽

Depth Of Field ◽

Computer Assisted ◽

Projection Data ◽

Two Dimensional ◽

Single Particles ◽

Dimensional Reconstruction ◽

Computer Assisted Image ◽

The Fourier Transform ◽

Space Approach

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.

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Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128961 ◽

1987 ◽

Vol 45 ◽

pp. 936-937

Author(s):

Neng-Yu Zhang ◽

Terence Wagenknecht ◽

Michael Radermacher ◽

Tom Obrig ◽

Joachim Frank

Keyword(s):

Three Dimensional ◽

Ribosomal Subunit ◽

Uranyl Acetate ◽

Reconstruction Method ◽

Single Exposure ◽

Electron Dose ◽

Ribosomal Subunits ◽

40S Ribosomal Subunit ◽

Dimensional Reconstruction ◽

Rabbit Reticulocytes

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.

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Three-Dimensional Structure of Cloned T3 Connector Protein at 1.6nm Resolution

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100180161 ◽

1990 ◽

Vol 48 (1) ◽

pp. 282-283

Author(s):

José L. Carrascosa ◽

José M. Valpuesta ◽

Hisao Fujisawa

Keyword(s):

Dna Sequences ◽

Expression Vector ◽

Three Dimensional ◽

Deae Cellulose ◽

Dna Packaging ◽

Dimensional Structure ◽

Two Dimensional ◽

Freezing And Thawing ◽

Three Dimensional Structure ◽

Dimensional Reconstruction

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.

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Three-Dimensional Reconstruction of Two Forms of the Chaperonin GRO EL

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100180045 ◽

1990 ◽

Vol 48 (1) ◽

pp. 258-259

Author(s):

J.L. Carrascosa ◽

G. Abella ◽

S. Marco ◽

M. Muyal ◽

J.M. Carazo

Keyword(s):

Three Dimensional ◽

The Other ◽

Two Dimensional ◽

Series Method ◽

Three Dimensional Reconstruction ◽

Structural Components ◽

E Coli ◽

Dimensional Reconstruction ◽

Morphogenetic Factors ◽

Tilt Series

Chaperonins are a class of proteins characterized by their role as morphogenetic factors. They trantsiently interact with the structural components of certain biological aggregates (viruses, enzymes etc), promoting their correct folding, assembly and, eventually transport. The groEL factor from E. coli is a conspicuous member of the chaperonins, as it promotes the assembly and morphogenesis of bacterial oligomers and/viral structures.We have studied groEL-like factors from two different bacteria:E. coli and B.subtilis. These factors share common morphological features , showing two different views: one is 6-fold, while the other shows 7 morphological units. There is also a correlation between the presence of a dominant 6-fold view and the fact of both bacteria been grown at low temperature (32°C), while the 7-fold is the main view at higher temperatures (42°C). As the two-dimensional projections of groEL were difficult to interprete, we studied their three-dimensional reconstruction by the random conical tilt series method from negatively stained particles.

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