scholarly journals Development of a Portable Multi-Sensor Urine Test and Data Collection Platform for Risk Assessment of Kidney Stone Formation

Electronics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2180
Author(s):  
Wen-Yaw Chung ◽  
Roozbeh Falah Ramezani ◽  
Angelito A. Silverio ◽  
Vincent F. Tsai
Keyword(s):  
Risk Assessment ◽  
Uric Acid ◽  
Data Collection ◽  
Stone Formation ◽  
Urinary Stone ◽  
Measurement Range ◽  
Urine Ph ◽  
System Validation ◽  
Stone Former ◽  
And Gender

In this paper, we present an Internet of things (IoT)-based data collection system for the risk assessment of urinary stone formation, or urolithiasis, by the measurement and storage of four parameters in urine: pH, concentrations of ionized calcium (Ca2+), uric acid and total dissolved solids. The measurements collected by the system from patients and healthy individuals grouped by age and gender will be stored in a cloud database. These will be used in the training phase of an artificial intelligence (AI) machine learning process utilizing the logistics regression model. The trained model provides a binary risk assessment, indicating if the end user is either a stone-former or not. For system validation, standard chemical solutions were used. Preliminary results indicated a sufficient measurement range, falling within the physiological range, and resolution for pH (2.0–10.0, +/−0.1), Ca2+(0.1–3.0 mmol/l, +/−0.05), uric acid (20–500 ppm, +/−1) and conductivity (1.0–40.0 mS/cm, +/−0.1), exhibiting high correlation with standard instruments. We intend to deploy this system in few hospitals in Taiwan to collect the data of patients’ urine, with analysis aided by urologist assessments for the risk of urolithiasis. The modularized design allows future modification and expansion to accommodate other sensing analytes.

scholarly journals Composition and Morphology of Nanocrystals in Urines of Lithogenic Patients and Healthy Persons

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Bao-Song Gui ◽  
Rong Xie ◽  
Xiu-Qiong Yao ◽  
Mei-Ru Li ◽  
Jian-Ming Ouyang
Keyword(s):  
Uric Acid ◽  
Calcium Oxalate ◽  
Stone Formation ◽  
Calcium Oxalate Monohydrate ◽  
Urinary Stone ◽  
Calcium Oxalate Dihydrate ◽  
Transmission Electron ◽  
Mean Size ◽  
Healthy Persons ◽  
Healthy Urine

The composition and morphology of nanocrystals in urines of healthy persons and lithogenic patients were comparatively investigated by means of X-ray diffraction (XRD) and transmission electron microscopy (TEM). It was shown that the main composition of urinary nanocrystals in healthy persons were calcium oxalate dihydrate (COD), uric acid, and ammonium magnesium phosphate (struvite). However, the main compositions of urinary nanocrystals in lithogenic patients were struvite,β-tricalcium phosphate, uric acid, COD, and calcium oxalate monohydrate (COM). According to the XRD data, the size of nanocrystals was calculated to be23∼72 nm in healthy urine and12∼118 nm in lithogenic urine by Scherer formula. TEM results showed that the nanocrystals in healthy urine were dispersive and uniform with a mean size of about 38 nm. In contrast, the nanocrystals in lithogenic urine were much aggregated with a mean size of about 55 nm. The results in this work indicated that the urinary stone formation may be prevented by diminishing the aggregation and the size differentiation of urinary nanocrystals by physical or chemical methods.

Epitaxial Relationships between Uric Acid Crystals and Mineral Surfaces:  A Factor in Urinary Stone Formation

Langmuir ◽  
2004 ◽  
Vol 20 (16) ◽  
pp. 6524-6529 ◽  
Author(s):  
M. Crina Frincu ◽  
Caitlin E. Fogarty ◽  
Jennifer A. Swift
Keyword(s):  
Uric Acid ◽  
Stone Formation ◽  
Urinary Stone ◽  
Mineral Surfaces

scholarly journals Biochemical urinalysis of healthy kidney and stone-generating kidney in unilateral urolithiasis

2019 ◽  
Vol 8 (2) ◽  
pp. 151-156
Author(s):  
Feramarz Mohammadalibeigi ◽  
Majid Shirani ◽  
Hamed Seyed-Salehi ◽  
Lotfollah Afzali
Keyword(s):  
Uric Acid ◽  
Kidney Stone ◽  
Stone Formation ◽  
Urinary Stone ◽  
Metabolic Factors ◽  
Cross Sectional ◽  
Urinary Sample ◽  
The Mean ◽  
The Difference ◽  
Southwest Iran

Introduction: Oxalate, calcium, uric acid, and citrate are among the most widely known biochemical factors for urinary stone formation. In most cases, urolithiasis occurs as unilateral despite the role of systemic metabolic factors in kidney stone formation. Objectives: The present research aimed to compare these urinary biochemical factors in healthy and stone-generating kidneys in patients with unilateral urolithiasis. Patients and Methods: Forty patients with unilateral urolithiasis participated in this cross-sectional, descriptive-analytical study. The patients were hospitalized in kidney surgery and kidney urology ward of Kashani hospital of Shahrekord, southwest Iran. After the implementation of crushing stone using transurethral lithotripsy (TUL), 5-10 cc of urinary sample was collected from each kidney. An AutoAnalyzer (Mindray Company, Bs–360 model) and laboratory kits (Bionic Company) were used to measure calcium, uric acid, and creatinine. Moreover, a manual method and Darman Faraz Kave company kits were applied to measure the levels of oxalate and citrate. Results on the healthy and stone-generating kidneys of each patient were separately analyzed using the Stata 13 software. Results: The difference in the mean ratio of uric acid, oxalate, and citrate to creatinine in the healthy kidneys and stone-generating kidneys was not significant. However, the mean UCa/UCr ratio in the healthy kidneys was 0.27±0.07 and relatively greater than that in the stone-generating kidneys (0.11±0.04) (P=0.06). Conclusion: The studied topical factors and secretory disorders had not any significant relationship with unilateral urolithiasis. The cause of unilateral urolithiasis should be searched in other factors such as metabolic factors, main positions of individual during 24 hours and sleeping, and anatomic disorders of kidney stone, or multifactorial.

Crystalluria

2015 ◽  
Vol 53 (s2) ◽  
Author(s):  
Michel Daudon ◽  
Vincent Frochot
Keyword(s):  
Metabolic Disorders ◽  
Calcium Phosphates ◽  
Stone Formation ◽  
Polarized Light ◽  
Urinary Stone ◽  
Urine Ph ◽  
Accurate Knowledge ◽  
Calcium Oxalates ◽  
Function Impairment ◽  
Comprehensive Examination

AbstractCrystalluria is a marker of urine supersaturation with substances deriving from metabolic disorders, inherited diseases or drugs. The investigation of crystalluria must be done according to a protocol which includes the delivery to the laboratory of a proper urine sample, the use of a microscope equipped with polarized light, the accurate knowledge of urine pH, and a comprehensive examination of the crystals, which is based on their identification, quantification and size measurement. For unusual crystals, infrared spectroscopy may also be needed. The main urinary crystalline categories include: calcium oxalates, calcium phosphates, uric acids and urates, struvite, aminoacids (cystine), purines (2,8-dihydroxyadenine and xanthine) and drugs (e.g. sulfamethoxazole, amoxycillin, ceftriaxone, atazanavir). The investigation of crystalluria is a cheap and valuable tool for the detection and the monitoring of inherited and acquired diseases associated with urinary stone formation or renal function impairment – either acute or chronic – due to intrarenal crystal precipitation.

Uric acid stones

2018 ◽  
Author(s):  
Michel Daudon ◽  
Paul Jungers
Keyword(s):  
Type 2 Diabetes ◽  
Uric Acid ◽  
Stone Formation ◽  
Urine Volume ◽  
Potassium Citrate ◽  
Urine Ph ◽  
The Metabolic Syndrome ◽  
Urine Alkalinization ◽  
Recurrent Stone

Uric acid (UA) stones are typically red-orange and often appear as sand/ gravel though they may be large. They are totally radiolucent. They account for about 10% of all kidney stones in most countries, and up to 20% in some populations. It is twice as frequent in males, prevalence increases with age, and it is two to three times higher in patients with type 2 diabetes or with features of the metabolic syndrome. Factors that induce the formation of UA stones are a low urine volume, hyperuricosuria, and, more importantly, a permanently low urine pH (< 5). Indeed, below its pKa of 5.35 at 37°C, UA is in non-dissociated form, whose solubility is at best 100 mg/L, whereas urinary UA excretion normally exceeds 600 mg/day and may exceed 1g/day.Because UA solubility increases up to approximately 500 mg/L at urine pH > 6, urine alkalinization, with a target pH of 6.5–7, is the cornerstone of medical treatment. This most often allows dissolution of existing stones and prevention of recurrent stone formation so that urological intervention is infrequently needed. The preferred agent for alkalinization is potassium citrate (30–60 mEq/day in divided doses), because potassium urate is twice more soluble than sodium urate. However, in patients with poor gastric tolerance to potassium citrate or contraindication to potassium supplements, sodium bicarbonate is an acceptable alternative. Limitation of animal proteins, purine-rich foods (including beer), alcoholic drinks and acidified beverages (sodas) are useful measures, together with large fluid intake (> 2–2.5 L/day). Allopurinol may be indicated in cases of symptomatic hyperuricaemia. Regular observance of alkalinisation, with periodic controls of urine pH by the patient, is needed to prevent the rapid formation of UA stones. Patients affected by UANL, especially if overweight, should be evaluated for type 2 diabetes or glucose intolerance and managed accordingly.

PD51-12 LOW URINE PH AND ASSOCIATED HYPOCITRATURIA ARE RISK FACTORS FOR URINARY STONE FORMATION IN ELDERLY STONE PATIENTS

2015 ◽  
Vol 193 (4S) ◽  
Author(s):  
Young-Won Kim ◽  
Sung Pil Seo ◽  
Yunbyung Chae ◽  
In-Chang Cho ◽  
Hoon Jang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Stone Formation ◽  
Urinary Stone ◽  
Urine Ph

scholarly journals Proteus mirabilis Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity

2021 ◽  
Vol 22 (13) ◽  
pp. 7205
Author(s):  
Matheus V. C. Grahl ◽  
Augusto F. Uberti ◽  
Valquiria Broll ◽  
Paula Bacaicoa-Caruso ◽  
Evelin F. Meirelles ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Stone Formation ◽  
Cell Types ◽  
Bioinformatic Analysis ◽  
Urinary Stone ◽  
Enzymatic Properties ◽  
Hek293 Cells ◽  
Urinary Stones ◽  
Isolated Cells ◽  
Tnf Α

Infection by Proteus mirabilis causes urinary stones and catheter incrustation due to ammonia formed by urease (PMU), one of its virulence factors. Non-enzymatic properties, such as pro-inflammatory and neurotoxic activities, were previously reported for distinct ureases, including that of the gastric pathogen Helicobacter pylori. Here, PMU was assayed on isolated cells to evaluate its non-enzymatic properties. Purified PMU (nanomolar range) was tested in human (platelets, HEK293 and SH-SY5Y) cells, and in murine microglia (BV-2). PMU promoted platelet aggregation. It did not affect cellular viability and no ammonia was detected in the cultures’ supernatants. PMU-treated HEK293 cells acquired a pro-inflammatory phenotype, producing reactive oxygen species (ROS) and cytokines IL-1β and TNF-α. SH-SY5Y cells stimulated with PMU showed high levels of intracellular Ca2+ and ROS production, but unlike BV-2 cells, SH-SY5Y did not synthesize TNF-α and IL-1β. Texas Red-labeled PMU was found in the cytoplasm and in the nucleus of all cell types. Bioinformatic analysis revealed two bipartite nuclear localization sequences in PMU. We have shown that PMU, besides urinary stone formation, can potentially contribute in other ways to pathogenesis. Our data suggest that PMU triggers pro-inflammatory effects and may affect cells beyond the renal system, indicating a possible role in extra-urinary diseases.

scholarly journals The Impact of Diet on Urinary Risk Factors for Cystine Stone Formation

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 528
Author(s):  
Roswitha Siener ◽  
Norman Bitterlich ◽  
Hubert Birwé ◽  
Albrecht Hesse
Keyword(s):  
Risk Factors ◽  
Dietary Habits ◽  
Sodium Intake ◽  
Stone Formation ◽  
Dietary Treatment ◽  
Vegetarian Diet ◽  
Dietary Sodium ◽  
Urine Ph ◽  
Cystine Stone ◽  
The Impact

Despite the importance of dietary management of cystinuria, data on the contribution of diet to urinary risk factors for cystine stone formation are limited. Studies on the physiological effects of diet on urinary cystine and cysteine excretion are lacking. Accordingly, 10 healthy men received three standardized diets for a period of five days each and collected daily 24 h urine. The Western-type diet (WD; 95 g/day protein) corresponded to usual dietary habits, whereas the mixed diet (MD; 65 g/day protein) and lacto-ovo-vegetarian diet (VD; 65 g/day protein) were calculated according to dietary reference intakes. With intake of the VD, urinary cystine and cysteine excretion decreased by 22 and 15%, respectively, compared to the WD, although the differences were not statistically significant. Urine pH was significantly highest on the VD. Regression analysis showed that urinary phosphate was significantly associated with cystine excretion, while urinary sulfate was a predictor of cysteine excretion. Neither urinary cystine nor cysteine excretion was affected by dietary sodium intake. A lacto-ovo-vegetarian diet is particularly suitable for the dietary treatment of cystinuria, since the additional alkali load may reduce the amount of required alkalizing agents.

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