scholarly journals Product Inhibition of Biological Hydrogen Production in Batch Reactors

Energies ◽  
2020 ◽  
Vol 13 (6) ◽  
pp. 1318 ◽  
Author(s):  
Subhashis Das ◽  
Rajnish Kaur Calay ◽  
Ranjana Chowdhury ◽  
Kaustav Nath ◽  
Fasil Ejigu Eregno
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Hydrogen Concentration ◽  
Microbial Growth ◽  
Batch Reactor ◽  
Product Inhibition ◽  
Batch Reactors ◽  
Hydrogen Production Rate ◽  
Biological Hydrogen Production ◽  
Fermentative Hydrogen Production

In this paper, the inhibitory effects of added hydrogen in reactor headspace on fermentative hydrogen production from acidogenesis of glucose by a bacterium, Clostridium acetobutylicum, was investigated experimentally in a batch reactor. It was observed that hydrogen itself became an acute inhibitor of hydrogen production if it accumulated excessively in the reactor headspace. A mathematical model to simulate and predict biological hydrogen production process was developed. The Monod model, which is a simple growth model, was modified to take inhibition kinetics on microbial growth into account. The modified model was then used to investigate the effect of hydrogen concentration on microbial growth and production rate of hydrogen. The inhibition was moderate as hydrogen concentration increased from 10% to 30% (v/v). However, a strong inhibition in microbial growth and hydrogen production rate was observed as the addition of H2 increased from 30% to 40% (v/v). Practically, an extended lag in microbial growth and considerably low hydrogen production rate were detected when 50% (v/v) of the reactor headspace was filled with hydrogen. The maximum specific growth rate (µmax), substrate saturation constant (ks), a critical hydrogen concentration at which microbial growth ceased (H2*) and degree of inhibition were found to be 0.976 h−1, 0.63 ± 0.01 gL, 24.74 mM, and 0.4786, respectively.

High yield hydrogen production in a single-chamber membrane-less microbial electrolysis cell

2010 ◽  
Vol 61 (3) ◽  
pp. 721-727 ◽  
Author(s):  
Yejie Ye ◽  
Liyong Wang ◽  
Yingwen Chen ◽  
Shemin Zhu ◽  
Shubao Shen
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Hydrogen Concentration ◽  
Current Density ◽  
Production Performance ◽  
Electrolysis Cell ◽  
Hydrogen Production Rate ◽  
High Hydrogen ◽  
Single Chamber ◽  
Hydrogen Recovery

The single-chamber membrane-less MEC exerted much better hydrogen production performance while given higher applied voltages than it did at lower. High applied voltages that could shorten the reaction time and the exposure of anode to air for at least 30 min between cycles can significantly suppress methanogen and increase hydrogen production. At an applied voltage of 1.0 V, a hydrogen production rate of 1.02 m3/m3/day with a current density of 5.7 A/m2 was achieved. Cathodic hydrogen recovery and coulombic efficiency were 63.4% and 69.3% respectively. The hydrogen concentration of mixture gas produced of 98.4% was obtained at 1.0 V, which was the best result of reports. The reasons that such a high hydrogen concentration can be achieved were probably the high electrochemical activity and hydrogen production capability of the active microorganisms. Increase in substrate concentrations could not improve MEC's performance, but increased the reaction times. Further, reactor configuration and operation factors optimisation should be considered to increase current density, hydrogen production rate and hydrogen recovery.

Effect of Sulfate Concentration on Biohydrogen Production by Enriched Anaerobic Sludge

2014 ◽  
Vol 884-885 ◽  
pp. 433-436 ◽  
Author(s):  
Bo Wang ◽  
Ya Nan Yin ◽  
Rong Cheng ◽  
Qiong Zhang ◽  
Liang Wang ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Experimental Results ◽  
Anaerobic Sludge ◽  
Hydrogen Yield ◽  
Sulfate Concentration ◽  
Hydrogen Production Rate ◽  
Fermentative Hydrogen Production ◽  
Initial Ph ◽  
Fermentative Hydrogen

The effect of SO2-4 concentration ranging from 0 to 10 g/L on fermentative hydrogen production by enriched anaerobic sludge was investigated using glucose as substrate at 35°C and initial pH 7.0. The experimental results showed that the hydrogen yield increased with increasing SO2-4 concentration from 0 to 0.05 g/L. The maximum maximum hydrogen yield of 272.2 mL/g glucose were obtained at the SO2-4 concentration of 0.05 g/L. The average hydrogen production rate increased with increasing SO2-4 concentration from 0 to 0.1 g/L and the maximum average hydrogen production rate of 8.4 mL/h was obtained at the SO2-4 concentration of 0.1 g/L. The Han-Levenspiel model could describe the effect of SO2-4 concentration on average hydrogen production rate successfully.

scholarly journals Enhanced hydrogen production of Rhodobacter sphaeroides promoted by extracellular H+ of Halobacterium salinarum

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Jiang-Yu Ye ◽  
Yue Pan ◽  
Yong Wang ◽  
Yi-Chao Wang
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Rhodobacter Sphaeroides ◽  
Coupled System ◽  
Purple Membrane ◽  
Halobacterium Salinarum ◽  
Control Group ◽  
Hydrogen Production Rate ◽  
Production Of Hydrogen ◽  
The Impact

Abstract Purpose This study utilized the principle that the bacteriorhodopsin (BR) produced by Halobacterium salinarum could increase the hydrogen production of Rhodobacter sphaeroides. H. salinarum are co-cultured with R. sphaeroides to determine the impact of purple membrane fragments (PM) on R. sphaeroides and improve its hydrogen production capacity. Methods In this study, low-salinity in 14 % NaCl domesticates H salinarum. Then, 0–160 nmol of different concentration gradient groups of bacteriorhodopsin (BR) and R. sphaeroides was co-cultivated, and the hydrogen production and pH are measured; then, R. sphaeroides and immobilized BR of different concentrations are used to produce hydrogen to detect the amount of hydrogen. Two-chamber microbial hydrogen production system with proton exchange membrane-assisted proton flow was established, and the system was operated. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. Results H salinarum can still grow well after low salt in 14% NaCl domestication. When the BR concentration is 80 nmol, the highest hydrogen production reached 217 mL per hour. Both immobilized PC (packed cells) and immobilized PM (purple membrane) of H. salinarum could promote hydrogen production of R. sphaeroides to some extent. The highest production of hydrogen was obtained by the coupled system with 40 nmol BR of immobilized PC, which increased from 127 to 232 mL, and the maximum H2 production rate was 18.2 mL−1 h−1 L culture. In the 192 h experiment time, when the potential is 0.3 V, the hydrogen production amount can reach 920 mL, which is 50.3% higher than the control group. Conclusions The stability of the system greatly improved after PC was immobilized, and the time for hydrogen production of R. sphaeroides significantly extended on same condition. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. These results are helpful to build a hydrogen production-coupled system by nitrogenase of R. sphaeroides and proton pump of H. salinarum. Graphical abstract

scholarly journals Anaerobic treatment of glycerol for methane and hydrogen production

2013 ◽  
Vol 14 (2) ◽  
pp. 149-156 ◽  
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Anaerobic Treatment ◽  
Microbial Culture ◽  
Batch Reactors ◽  
Continuous Stirred Tank Reactor ◽  
Organic Loading ◽  
Loading Rates ◽  
Fermentative Hydrogen Production ◽  
Initial Ph

This work focused on glycerol exploitation for biogas and hydrogen production. Anaerobic digestion of pure glycerol was studied in a continuous stirred tank reactor (CSTR), operated under mesophilic conditions (35oC) at various organic loading rates. The overall operation of the reactor showed that it could not withstand organic loading rates above 0.25 g COD L-1 d-1, where the maximum biogas (0.42 ± 0.05 L (g COD)-1) and methane (0.30 ± 0.04 L (g COD)-1) production were achieved. Fermentative hydrogen production was carried out in batch reactors under mesophilic conditions (35oC), using heat-pretreated anaerobic microbial culture as inoculum. The effects of initial concentration of glycerol and initial pH value on hydrogen production were studied. The highest yield obtained was 22.14 ± 0.46 mL H2 (g COD added)-1 for an initial pH of 6.5 and an initial glycerol concentration of 8.3 g COD L-1. The main metabolic product was 1.3 propanediol (PDO), while butyric and acetic acids as well as ethanol, at lower concentrations, were also determined.

Effects of applied voltage on hydrogen production rate of a single reactor BML with Clostridium sp.

2015 ◽  
Vol 98 ◽  
pp. 383-389 ◽  
Author(s):  
Chiung-Yi Cheng ◽  
Kuang-Li Cheng ◽  
Terng-Jou Wan ◽  
Wei-Nung Kuo ◽  
Feng-Jen Chu ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Applied Voltage ◽  
Hydrogen Production Rate

Continuous Fermentative Hydrogen Production from Brown Sugar Using EGSB Reactor

2010 ◽  
Vol 113-116 ◽  
pp. 1132-1137 ◽  
Author(s):  
Yong Feng Li ◽  
Yong Ming Hui ◽  
Xin Yao ◽  
Lu Wang ◽  
Qian Wen Song ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Hydraulic Retention Time ◽  
Loading Rate ◽  
Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Hydrogen Production Rate ◽  
Brown Sugar ◽  
Fermentative Hydrogen Production ◽  
Fermentative Hydrogen

In this experiment, brown sugar was chosen as the substrate of continuous operation. A lab-scale expanded granular sludge blanket (EGSB) reactor was employed. Stable ethanol-type fermentation was formed by controlling the organic loading rate (OLR). The results showed a maximum hydrogen production rate of 5.73L / L reactor•d was achieved, under the condition that the hydraulic retention time (HRT) = 2h, OLR = 97.2kg COD/m3 reactor•d. The average hydrogen content in the biogas during the 73-day operation was 41.27%.

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