scholarly journals Improvement of Biogas Yield by Pre-Treating Poultry Waste with Bacterial Strains

Energies ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5601
Author(s):  
Krystyna Cybulska ◽  
Ilona Kołosowska ◽  
Karol Kramkowski ◽  
Monika Karpińska ◽  
Katarzyna Roszkowicz-Ostrowska ◽  
...  

Poultry waste is increasingly used as a substrate for the methane fermentation process in a biogas plant. However, not all waste materials processed in the meat industry meet the criteria for optimal process management and cost-effective methane efficiency. An example may be centrifuged biological sludge, etc. Treatment of such material used as a substrate by introducing new metabolically and enzymatically active strains of bacteria could be beneficial for the fermentation process in a biogas plant and provide increased energy efficiency. The aim of the study was to compare the amount and quality of biogas obtained from biological sludge from the processing of poultry vaccinated with metabolically diversified bacterial inoculum after initial incubation of the batch before the actual process in a biofermenter. Laboratory tests were carried out in accordance with the guidelines contained in the DIN 38 414-S8 and VDI 4630 standards. Based on the obtained results, it was found that the optimized biological sludge can be used as a substrate in the methane fermentation process in a biogas plant. The material processed by the combination of bacterial strains marked with the symbols A/C, E/G, and F/H showed a significantly increased efficiency of biogas, including methane, compared to the non-grafted material. This is a good predictor for industrial applications, process feasibility, economic viability, and environmental sustainability that should be compiled based on the results obtained from this study.

2014 ◽  
Vol 25 (4) ◽  
pp. 55-58
Author(s):  
Anna Kwarciak-Kozłowska ◽  
Aleksandra Krzywicka ◽  
Lucyna Sławik-Dembiczak

Abstract The aim of this paper was to study meat industry wastewater treatment efficiency during fermentation process in ASBR reactor and post-treatment in UF process. The anaerobic process obtained a considerable degree of the removal of organic pollutants from raw wastewater designated as COD (73.3%), BOD (71.4%) and TOC (83.2%). The concentrations of COD and BOD were 435 and 443 mg/dm3, respectively. The value of TOC reached a level of 136 mg/dm3. Generated biogas in the methane fermentation process of wastewater from meat industry plants was characterized by high methane content (80.9% vol.). In the final part of the experiment, the UF process was used in order to post-treating effluent from ASBR reactor. During the UF process, COD, BOD and TOC parameters were removed at 67.2%, 68% and 70.4%, respectively.


2015 ◽  
Vol 36 (1) ◽  
pp. 101-112 ◽  
Author(s):  
Julia V. Karaeva ◽  
Galia R. Khalitova ◽  
Dmitry A. Kovalev ◽  
Irene A. Trakhunova

Abstract Two systems of hydraulic mixing in a vertical cylindrical anaerobic digester: standard and modernised are discussed in the paper. Numerical investigations that were carried out are focused on a study of hydrodynamic processes in an aerobic digester using two various systems of hydraulic mixing as well as on analysis of the efficiency of methane fermentation process accomplished under different geometric parameters of an anaerobic digester and systems of hydraulic mixing.


Author(s):  
Akinori IGUCHI ◽  
Yuya KANO ◽  
Yuki IKARASHI ◽  
Ayaka KAINUMA ◽  
Saori HORI ◽  
...  

2015 ◽  
Vol 18 (2) ◽  
pp. 239-247 ◽  
Author(s):  
Anh Van Ngo ◽  
Ha Thi Nguyen ◽  
Chieu Van Le ◽  
Rajeev Goel ◽  
Mitsuharu Terashima ◽  
...  

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1227
Author(s):  
Robert H. Hicks ◽  
Mauro Moreno-Beltrán ◽  
Deborah Gore-Lloyd ◽  
Christopher J. Chuck ◽  
Daniel A. Henk

Metschnikowia pulcherrima is a non-conventional yeast with potential to be used in biotechnological processes, especially those involving low-cost feedstock exploitation and biocontrol applications. The combination of traits that supports these industrial applications in M. pulcherrima also makes it an attractive option to study in the context of livestock health. In this study, we examined the specific interactions between M. pulcherrima and multiple avian pathogenic bacteria. We tested individual bacteria–yeast interactions and bacterial combinations in both solid and liquid media and in variable nutrient environments. Across multiple isolates of M. pulcherrima, we observed different levels of antimicrobial activity, varying from supporting the growth of competing bacteria through suppression and bacterial killing, and we found that these responses varied depending on the bacterial strains and media. We identified multiple molecular routes, including proteins produced by M. pulcherrima strains, that acted to control these microbial interactions. Furthermore, protein screening revealed that M. pulcherrima strains were induced to produce proteins specifically when exposed to bacterial strains, suggesting that fine-tuned mechanisms allow M. pulcherrima to function as a potential lynchpin in a microbial community.


2021 ◽  
Author(s):  
Michael Bamitale Osho ◽  
Sarafadeen Olateju Kareem

Biotransformation of broth through fermentation process suffers a major setback when it comes to disintegration of organic substrates by microbial agents for industrial applications. These biocatalysts are in crude/dilute form hence needs to be purified to remove colloidal particles and enzymatic impurities thus enhancing maximum activity. Several contractual procedures of concentrating dilute enzymes and proteins had been reported. Such inorganic materials include ammonium sulphate precipitation; salting, synthetic polyacrylic acid; carboxy-methyl cellulose, tannic acid, edible gum and some organic solvents as precipitants etc. The emergence of organic absorbents such as sodom apple (Calostropis procera) extract, activated charcoal and imarsil had resulted in making significant impact in industrial circle. Various concentrations of these organic extracts have been used as purifying agents on different types of enzyme vis: lipase, amylase, protease, cellulase etc. Purification fold and stability of the enzyme crude form attained unprecedented results.


2019 ◽  
Vol 116 ◽  
pp. 00104
Author(s):  
Iwona Zawieja ◽  
Kinga Brzeska

The advanced oxidation processes (AOPs) play an important role in the degradation of hardly decomposable organic pollutants. AOPs methods rely on the production of highly reactive hydroxyl OH• radicals. The aim of the conducted research was to intensify biogas production in the methane fermentation process of excess sludge subjected to the process of deep oxidation with Fenton's reagent. In the process of oxidation of sewage sludge with the Fenton reagent, doses of iron ions in the range 0.02–0.14 g Fe2+/g TS (total solids) were used Hydrogen peroxide was measured in the proportions 1: 1–1:10 in relation to the mass of iron ions. The basic substrate of the study was excess sludge. In the case of excess sludge oxidation with the use of Fenton's reagent, the most favorable process conditions were considered to be the dose of iron ions 0.08 g Fe2+/g d.m. and a Fe2+: H2O2 ratio of 1:5. As a result of subjecting the excess sludge to disintegration with the Fenton reagent in the above-mentioned dose, with respect to the fermentation process of unprocessed sludge, about two-fold increase in the digestion degree of excess sludge and about 35% increase of the biogas yield was obtained.


2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Li-Tong Ma ◽  
Ya-Nan Lu ◽  
Ya-Xiong Wang

The effects of acid precipitation with different liquid acids on spectral properties of fulvic acid extracted from peat was studied to select the most appropriate liquid acid for the acid precipitation step of the alkali solution acid precipitation method, and the new process mechanism of peat utilization by coupling methane fermentation and extraction of fulvic acid was analyzed. The alkali solution acid precipitation method was adopted to extract fulvic acid from the methane-fermented group and methane-unfermented group. Different liquid acids were used to conduct acid precipitation during the extraction. And then, the characterizations of fulvic acid samples were conducted through Fourier infrared spectroscopy, UV-Vis spectroscopy, and fluorescence spectroscopy. The yield and content of fulvic acid decreased significantly after methane fermentation. During the methane fermentation process, some fulvic acid was consumed and utilized by microorganisms to promote the methane fermentation process, resulting both in the decrease of methyl, hydroxyl, and ether bonds and in the increase of methylene, carbonyl, conjugated double bond, benzene rings, and other groups. The E4/E6 ratio shows that the E4/E6 ratio of fulvic acid was decreased after methane fermentation, and the fermentation consumed the functional group with simpler structure such as aliphatic chain hydrocarbon of fulvic acid while the structure with a higher degree of aromatization and conjugate cannot be consumed. When conducting acid precipitation with different liquid acids, the yield of fulvic acid was the highest through acid precipitation with phosphoric acid. The fulvic acid obtained through acid precipitation with nitric acid has a higher content, more benzene rings, and the highest degree of aromatization.


2020 ◽  
pp. 004051752096828
Author(s):  
Lifeng Cheng ◽  
Shengwen Duan ◽  
Xiangyuan Feng ◽  
Ke Zheng ◽  
Qi Yang ◽  
...  

To explore high-quality microbial resources with the capability of ramie degumming, we collected soil samples from rotten ramie and straw heaps. After enrichment culture by ramie raw materials, bacterial strains with the potential ramie-degumming function were screened using a pectin-hydrolysis plate. Dominant bacteria were identified by combining colonial morphological characteristics with the molecular biology method, and their ramie-degumming effects were verified through comprehensive biological degumming indices. Results demonstrated that Bacillus aryabhattai, Bacillus thuringiensis, Lysinibacillus fusiformis, and Acidovorax temperans were successfully obtained. The highest pectinase activity, 98.2 U/mg, was found by A. temperans. B. thuringiensis showed the best ramie-degumming effect. The residual gum content, single-fiber linear density, and bundle-breaking strength of the degummed ramie fiber treated with B. thuringiensis were 8.32%, 6.80 dtex, and 7.84 cN/dtex, respectively. The residual gum content of the ramie fiber treated with B. thuringiensis met the textile requirement (<10%), and the values of all other indicators were also satisfactory. Therefore, B. thuringiensis was an excellent strain for ramie degumming, indicating potential industrial applications.


2018 ◽  
Vol 44 ◽  
pp. 00114 ◽  
Author(s):  
Natalia Mioduszewska ◽  
Mariusz Adamski ◽  
Anna Smurzyńska ◽  
Jacek Przybył ◽  
Krzysztof Pilarski

The aim of the study was to evaluate the usefulness of sugar beet for biogas production, taking into account the duration time of storage and sugar content in the roots. The research has included analysis of methane and biogas yield of sugar beet. The relations between the sugar content in the roots and the length of storage period and the course of the methane fermentation process were determined. Sugar beets with sugar content of 17.6% and 19.6% were used for this experiment. In order to analyse the fermentation process, the fresh beets and the beets stored in flexible, hermetic tanks in the period of 43 and 89 days were used. Based on the analysis of the obtained results, it was found that the sugar content and the storage time of sugar beet roots can differentiate the production of biomethane and that it influences the methane fermentation process and the quality of the produced biogas.


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