scholarly journals Pigment Production by the Edible Filamentous Fungus Neurospora Intermedia

Fermentation ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 11 ◽  
Author(s):  
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Author(s):  
Mostafa Jabbari ◽  
Osagie A. Osadolor ◽  
Ramkumar B. Nair ◽  
Mohammad J. Taherzadeh

All-polyamide composite coated fabric (APCCF) materials were developed and used as the material of construction of textile bioreactors as a replacement of traditional costly steel-/concreate-based bioreactors. Then, APCCF-bioreactor was used to cultivate filamentous fungus Neurospora intermedia to produce ethanol and fungal biomass. The results showed similar performance of the fungus cultivated in stain-less steel bioreactors. Techno-economical analysis for a 5000-m3 APCCF bioreactor for fermentation facility would lead to a reduction of the annual production cost of the facility by $128,000,000 compared to similar stainless-steel processes. The comparative analyses (including mechanical and morphological analyses, density measurements and techno-economical analysis) revealed that the APCCF is a better candidate for material of construction of the textile bioreactor. The APCCF is a 100% recyclable single polymer composite which was prepared from textile production line waste.



2000 ◽  
Vol 66 (3) ◽  
pp. 1120-1125 ◽  
Author(s):  
Hassan Hajjaj ◽  
Alain Klaébé ◽  
Gérard Goma ◽  
Philippe J. Blanc ◽  
Estelle Barbier ◽  
...  

ABSTRACT During submerged culture in the presence of glucose and glutamate, the filamentous fungus Monascus ruber produces water-soluble red pigments together with citrinin, a mycotoxin with nephrotoxic and hepatoxic effects on animals. Analysis of the13C-pigment molecules from mycelia cultivated with [1-13C]-, [2-13C]-, or [1,2-13C]acetate by 13C nuclear magnetic resonance indicated that the biosynthesis of the red pigments used both the polyketide pathway, to generate the chromophore structure, and the fatty acid synthesis pathway, to produce a medium-chain fatty acid (octanoic acid) which was then bound to the chromophore by atrans-esterification reaction. Hence, to enhance pigment production, we tried to short-circuit the de novo synthesis of medium-chain fatty acids by adding them to the culture broth. Of fatty acids with carbon chains ranging from 6 to 18 carbon atoms, only octanoic acid showed a 30 to 50% stimulation of red pigment production, by a mechanism which, in contrast to expectation, did not involve its direct trans-esterification on the chromophore backbone. However, the medium- and long-chain fatty acids tested were readily assimilated by the fungus, and in the case of fatty acids ranging from 8 to 12 carbon atoms, 30 to 40% of their initial amount transiently accumulated in the growth medium in the form of the corresponding methylketone 1 carbon unit shorter. Very interestingly, these fatty acids or their corresponding methylketones caused a strong reduction in, or even a complete inhibition of, citrinin production byM. ruber when they were added to the medium. Several data indicated that this effect could be due to the degradation of the newly synthesized citrinin (or an intermediate in the citrinin pathway) by hydrogen peroxide resulting from peroxisome proliferation induced by medium-chain fatty acids or methylketones.



2021 ◽  
Vol 146 ◽  
pp. 103488
Author(s):  
Midori Tabara ◽  
Hisashi Koiwa ◽  
Nobuhiro Suzuki ◽  
Toshiyuki Fukuhara


2021 ◽  
Vol 86 (3) ◽  
pp. 969-976
Author(s):  
Chuantao Zeng ◽  
Yumiko Yoshizaki ◽  
Xuan Yin ◽  
Zitai Wang ◽  
Kayu Okutsu ◽  
...  


Genetics ◽  
1975 ◽  
Vol 80 (4) ◽  
pp. 785-805
Author(s):  
P T Spieth

ABSTRACT Electrophoretically detectable variation in the fungus Neurospora intermedia has been surveyed among isolates from natural populations in Malaya, Papua, Australia and Florida. The principal result is a pattern of genetic variation within and between populations that is qualitatively no different than the well documented patterns for Drosophila and humans. In particular, there is a high level of genetic variation, the majority of which occurs at the level of local populations. Evidence is presented which argues that N. intermedia has a population structure analogous to that of an annual vascular plant with a high level of vegetative reproduction. Sexual reproduction appears to be a regular feature in the biology of the species. Substantial heterokaryon function seems unlikely in natural populations of N. intermedia. Theoretical considerations concerning the mechanisms underlying the observed pattern of variation most likely should be consistent with haploid selection theory. The implications of this constraint upon the theory are discussed in detail, leading to the presentation of a model based upon the concept of environmental heterogeneity. The essence of the model, which is equally applicable to haploid and diploid situations, is a shifting distribution of multiple adaptive niches among local populations such that a given population has a small net selective pressure in favor of one allele or another, depending upon its particular distribution of niches. Gene flow among neighboring populations with differing net selective pressures is postulated as the principal factor underlying intrapopulational allozyme variation.



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