scholarly journals A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2896
Author(s):  
Liangliang Zhu ◽  
Hongshun Hao ◽  
Chao Ding ◽  
Hanwei Gan ◽  
Shuting Jiang ◽  
...  

To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO3) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 101–1.3 × 107 CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes.

The Analyst ◽  
2015 ◽  
Vol 140 (19) ◽  
pp. 6748-6757 ◽  
Author(s):  
Xuanping Tan ◽  
Jidong Yang ◽  
Qin Li ◽  
Qiong Yang

This paper reports a quantum dot (QD)-based “off–on” fluorescent biosensor specifically for the determination of glutathione (GSH) with high sensitivity.


Small ◽  
2012 ◽  
Vol 8 (17) ◽  
pp. 2652-2658 ◽  
Author(s):  
P. Haro-González ◽  
L. Martínez-Maestro ◽  
I. R. Martín ◽  
J. García-Solé ◽  
D. Jaque

2018 ◽  
Vol 54 (20) ◽  
pp. 2542-2545 ◽  
Author(s):  
Yamin Xiong ◽  
Peijun Meng ◽  
Huiling Li ◽  
Yue Hu ◽  
Lihong Zhou ◽  
...  

A facile method was constructed and applied to detection of copper species in bio-samples with a tunable dynamic range.


NANO ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. 1750151 ◽  
Author(s):  
Yue Zheng ◽  
Hengshan Tang ◽  
Xiaoyu Wang ◽  
Ya Di ◽  
Kunling Lu ◽  
...  

The synthesis of aqueous MPA-capped CdTe quantum dots (QDs) via a facile one-pot route was developed. The particle size, optical properties and crystal structure of the as-synthesis QDs were investigated by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV-vis absorption and photoluminescence (PL) spectra, respectively. Meanwhile, the effect of reaction conditions, including reaction time, pH and the quantity of sodium citrate (SC) on the growth of CdTe QDs were discussed. Then, the obtained CdTe QDs were successfully used for the detection of trace Cu[Formula: see text] with high sensitivity and excellent selectivity. The fluorescence intensity of CdTe QDs with Cu[Formula: see text] concentration showed a linear relationship in the range from [Formula: see text] mol/L to [Formula: see text][Formula: see text]mol/L. The correlation coefficient (R) is 0.9980 and limit of detection (LOD) is [Formula: see text][Formula: see text]mol/L. Moreover, the concentration of Cu[Formula: see text] in tap water samples was determined based on this sensing system and the recovery test was satisfactory.


Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2017
Author(s):  
Chen Liu ◽  
Jialun Han ◽  
Lujian Zhou ◽  
Jingjing Zhang ◽  
Jie Du

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.


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