Optimized Extraction of Phenylpropanoids and Flavonoids from Lemon Verbena Leaves by Supercritical Fluid System Using Response Surface Methodology

The aim of this work was to optimize the recovery of phenolic compounds from Lippia citriodora using supercritical fluid extraction (SFE). To achieve this goal, response surface methodology based on a 23 central composite design was used to evaluate the effects of the following experimental factors: temperature, pressure and co-solvent percentage. The effects of these variables on the extraction yield and total polar compound contents were evaluated. With respect to the phytochemical composition, an exhaustive individual phenolic compound quantitation was carried out by HPLC-ESI-TOF-MS to analyze the functional ingredients produced by this system design. To the best of our knowledge, this is the first time that a standardized supercritical fluid process has been developed to obtain functional ingredients based on phenolic compounds from L. citriodora in which the individual compound concentration was monitored over the different SFE conditions. The results enabled the establishment of the optimal technical parameters for developing functional ingredients and revealed the main factors that should be included in the extraction process control. This functional food ingredient design could be used as a control system to be applied in nutraceutical and functional food production industry.

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Optimization of Supercritical Fluid Extraction of Phenolic Compounds from Peach Blossom (Amygdalus Persica) by Response Surface Methodology

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.17:180-187 ◽

2018 ◽

Vol 17 (2) ◽

pp. 180-187

Author(s):

Yang Wen-Bo ◽

Liu Jie-Chao ◽

Liu Hui ◽

Zhang Chun-Ling ◽

Lv Zhen-Zhen ◽

...

Keyword(s):

Response Surface Methodology ◽

Phenolic Compounds ◽

Response Surface ◽

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Antioxidant Activities ◽

Total Phenolic ◽

Fluid Extraction ◽

Phenolic Contents ◽

C 30

Supercritical fluid extraction (SFE) was used for the extraction of phenolic compounds from peach blossom (Amygdalus persica). The optimum conditions of SFE were optimized by response surface methodology and confirmed at 64°C, 30 MPa, 143 min, and 35 mL ethanol (100%) as modifier for maximum total phenolic contents (54.10 mg GAE g−1 DW), which was higher than the yield achieved by ultrasonic-assisted extraction (44.04 mg GAE g−1 DW). The antioxidant activities and tyrosinase inhibitory activities of extraction extracted by SFE were 79.55% and 14.42%. Furthermore, chlorogenic acid, quercetin-3-O-galactoside, kaempferol-3-O-galactoside, quercitrin-3-O-rhamnoside, and kaempferol 4′-glucoside were identified in this study.

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An Environment Friendly, Low-Cost Extraction Process of Phenolic Compounds from Grape Byproducts. Optimization by Multi-Response Surface Methodology

Food and Nutrition Sciences ◽

10.4236/fns.2013.46084 ◽

2013 ◽

Vol 04 (06) ◽

pp. 650-659 ◽

Cited By ~ 12

Author(s):

Hiba N. Rajha ◽

Nada El Darra ◽

Eugène Vorobiev ◽

Nicolas Louka ◽

Richard G. Maroun

Keyword(s):

Response Surface Methodology ◽

Phenolic Compounds ◽

Response Surface ◽

Low Cost ◽

Extraction Process ◽

Environment Friendly

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Determination of optimal parameters of basil supercritical fluid extraction by response surface methodology

Acta periodica technologica ◽

10.2298/apt1647193f ◽

2016 ◽

pp. 193-203

Author(s):

Snezana Filip ◽

Senka Vidovic ◽

Branimir Pavlic ◽

Zoran Zekovic

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Supercritical Fluid Extraction ◽

Supercritical Fluid ◽

Interactive Effect ◽

Extraction Process ◽

Extraction Yield ◽

Fluid Extraction ◽

Experimental Values

The supercritical fluid extraction of aroma compounds from basil (Ocimum basilicum L.) was studied. Response surface methodology was used to optimize the parameters of the process. Full factorial design was applied to evaluate the effects of two independent variables (pressure and temperature) on the extraction yield and linalool yield. From the response surface plots, pressure and temperature exhibited independent and interactive effect on the extraction yield. The optimal conditions to obtain the highest extraction yield (1.91%) of O. basilicum were the pressure of 29.7 MPa and temperature of 59.2oC, whereas the highest yield of linalool (1.998 g?kg-1) was obtained at the pressure of 20 MPa and temperature of 40oC. The experimental values agreed with the predicted ones, indicating suitability of the response surface methodology for optimizing the extraction process.

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Development of an Innovative Pressurized Liquid Extraction Procedure by Response Surface Methodology to Recover Bioactive Compounds from Carao Tree Seeds

Foods ◽

10.3390/foods10020398 ◽

2021 ◽

Vol 10 (2) ◽

pp. 398

Author(s):

Jhunior Abrahan Marcía Fuentes ◽

Lucía López-Salas ◽

Isabel Borrás-Linares ◽

Miguel Navarro-Alarcón ◽

Antonio Segura-Carretero ◽

...

Keyword(s):

Response Surface Methodology ◽

Phenolic Compounds ◽

Response Surface ◽

Bioactive Compounds ◽

Extraction Procedure ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Bioactive Substances ◽

Functional Ingredients ◽

Tree Seeds

Nowadays there are evidences from several studies which have revealed the protective effects of food against chronic diseases. These healthy properties have been related to bioactive compounds. Among bioactive substances, the scientific interest in phenolic compounds has stimulated multidisciplinary research on the composition of plant phenolic compounds. The aim of this work has been to determine the bioactive composition of Carao tree seeds (Cassia grandis) and to optimize the recovering of these compounds for developing functional ingredients. To achieve this goal, pressurized liquid extraction (PLE) has been applied to recover these phytochemicals. The optimization of this innovative extraction procedure was performed by a response surface methodology (RSM) based on a central composite design 23 model to address the bioactive compounds extraction. Phenolic compounds recovered by PLE were characterized using reversed-phase high-performance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS). Analytical characterization allowed the identification and quantitation of phenolic compounds belonging to hydroxybenzoic acids and flavonoids (flavonols, flavanols, flavanones and proanthocyanidins). Phytochemical concentrations were used as response variable in order to get the best extraction conditions. These results pointed out that Carao tree seeds can be a potential source of bioactive compounds and PLE extracts could be used as functional ingredients.

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