scholarly journals Albumin Microspheres as “Trans-Ferry-Beads” for Easy Cell Passaging in Cell Culture Technology

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 176
Author(s):  
Patrizia Favella ◽  
Susanne Sihler ◽  
Heinz Raber ◽  
Ann-Kathrin Kissmann ◽  
Markus Krämer ◽  
...  

Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the “trans-ferry-beads” presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.

2004 ◽  
Vol 847 ◽  
Author(s):  
Giulia Fornasieri ◽  
Stéphane Badaire ◽  
Rénal Vasco Backov ◽  
Philippe Poulin ◽  
Cécile Zakri ◽  
...  

Using reverse emulsion systems, we were able to trigger mineralization confined at an oil-water interface. In this process, the alcoxide silica precursor is dissolved in the oil continuous phase of the emulsion and diffuses through the bulk to the interface where it starts to hydrolyze and condense as soon as a certain concentration threshold is attained. The process takes place only in the presence of a water soluble surfactant inside the droplet. This surfactant leads to the presence of a controlled mesoporosity inside the silica shells. The obtained objects could be used in different encapsulation applications.


1985 ◽  
Vol 11 (6) ◽  
pp. 729-734 ◽  
Author(s):  
Manabu Yamaguchi ◽  
Atsushi Kobayashi ◽  
Kohzi Ohbori ◽  
Takashi Katayama

2017 ◽  
Vol 18 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Miki ITO ◽  
Midori UEHARA ◽  
Ryota WAKUI ◽  
Makoto SHIOTA ◽  
Takashi KUROIWA

1973 ◽  
Vol 1973 (1) ◽  
pp. 469-474 ◽  
Author(s):  
P. G. Jeffery

ABSTRACT This paper describes those considerations that are of importance in undertaking large-scale oil-spreading experiments. One such experiment involving a discharge of 120 tons of Iranian Light Crude Oil in the North Atlantic is described, and the spreading pattern observed is reported in detail. The observations continued over a period of four days, after which the bulk of the oil had disappeared by natural factors, leaving only a few patches of thicker oil, largely in the form of a water-in-oil emulsion, which was also rapidly disappearing. Blokker constants have been calculated for the observed spreading of the slick, and these are reasonably constant throughout the four-day period.


1987 ◽  
Vol 22 (3) ◽  
pp. 437-443 ◽  
Author(s):  
N. Kosaric ◽  
Z. Duvnjak

Abstract Aerobic sludge from a municipal activated sludge treatment plant, sludge from a conventional municipal anaerobic digester, aerobic sludge from an activated sludge process of a petroleum refinery, and granular sludge from an upflow sludge blanket reactor (USBR) were tested in the deemulsification of a water-in-oil emulsion. All sludges except the last one, showed a good deemulsification capability and could he used for a partial deemulsification of such emulsions. The rate and degree of the deemulsifications increased with an increase in sludge concentrations. The deemulsifications were faster at 85°C and required smaller amounts of sludge than in the case of the deemulsifications at room temperature. An extended stirring (up to a certain limit) in the course of the dispersion of sludge emulsion helped the deemulsification. Too vigorous agitation had an adverse effect. The deemulsification effect of sludge became less visible with an increase in the dilution of emulsion which caused an increase in its spontaneous deemulsification.


2012 ◽  
Vol 9 (1) ◽  
pp. 110-115
Author(s):  
L.A. Kovaleva ◽  
R.R. Zinnatullin ◽  
V.N. Blagochinnov ◽  
A.A. Musin ◽  
Yu.I. Fatkhullina ◽  
...  

Some results of experimental and numerical studies of the influence of radio-frequency (RF) and microwave (MW) electromagnetic (EM) fields on water-in-oil emulsions are presented. A detailed investigation of the dependence of the dielectric properties of emulsions on the frequency of the field makes it possible to establish the most effective frequency range of the EM influence. The results of water-in-oil emulsion stability in the RF EM field depending on their dielectric properties are presented. The effect of the MW EM field on the emulsion in a dynamic mode has been studied experimentally. In an attempt to understand the mechanism of emulsion destruction the mathematical model for a single emulsion droplet dynamics in radio-frequency (RF) and microwave (MW) electromagnetic fields is formulated.


2021 ◽  
Vol 167 ◽  
pp. 106900
Author(s):  
G. Sahasrabudhe ◽  
G. DeIuliis ◽  
J. Davy ◽  
K.P. Galvin

2021 ◽  
pp. 116680
Author(s):  
Hao Lu ◽  
Shihan Wu ◽  
Zhiqiang Miao ◽  
Xiao Xu ◽  
Yiqian Liu ◽  
...  

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