scholarly journals Cloning, Expression, and Tobacco Overexpression Analyses of a PISTILLATA/GLOBOSA-like (OfGLO1) Gene from Osmanthus fragrans

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1748
Author(s):  
Zhanghui Zeng ◽  
Si Chen ◽  
Mingrui Xu ◽  
Min Wang ◽  
Zhehao Chen ◽  
...  
Keyword(s):  
Ectopic Expression ◽  
Genetic Effect ◽  
Floral Organ ◽  
Plant Size ◽  
Mads Box ◽  
Flower Bud ◽  
Osmanthus Fragrans ◽  
Reading Frame ◽  
Full Flowering ◽  
Mads Box Gene

GLOBOSA (GLO), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in Osmanthus fragrans, which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous GLO1 gene (named OfGLO1) was cloned from a flower bud of O. fragrans using the RACE technique. The OfGLO1 has a 645 bp open reading frame, encoding 214 amino acids. Similar to other PI/GLO proteins, OfGLO1 has two conserved domains, MADS MEF2-like and K-box, and a 16-amino-acid PI motif in the C terminal region. Our phylogeny analysis classified OfGLO1 as a PI-type member of the B-class MADS-box gene family. The qRT-PCR assay showed that the expression of OfGLO1 in O. fragrans was continuously upregulated from the tight bud stage to the full flowering stage but barely expressed in the pistils, sepals, and non-floral organs, such as root, leaf, and stem. The genetic effect of OfGLO1 was assayed by ectopic expression in tobacco plants. Compared with the wild-type, OfGLO1 transformants showed reduced plant size, earlier flowering, shorter stamens, and lower seed setting rates. Furthermore, some stamens were changed into petal-like structures. These findings indicate that OfGLO1 plays an important role in the regulation of flower development. This study improved our understanding of class B gene function in woody plants.

scholarly journals A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 265
Author(s):  
Yong Zhou ◽  
Lingli Ge ◽  
Lifang Hu ◽  
Yingui Yang ◽  
Shiqiang Liu
Keyword(s):  
Functional Characterization ◽  
Ectopic Expression ◽  
Leaf Size ◽  
Reproductive Organs ◽  
Mads Box ◽  
Cucumis Sativus L ◽  
Reading Frame ◽  
Relationship Analysis ◽  
Mads Box Gene

The AGL15 subfamily MADS-box proteins play vital roles in various developmental processes, such as floral transition, somatic embryogenesis, and leaf and fruit development. In this work, an AtAGL15 ortholog, CsMADS26, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS26 is 669 bp in length, encoding a predicted protein of 222 amino acids. The CsMADS26 protein contains a highly conserved MADS-box domain and a variable C domain, as well as less conserved I and K domains. Phylogenetic relationship analysis revealed that CsMADS26 was clustered into the AGL15 clade of AGL15 subfamily. Expression analysis based on qRT-PCR showed that CsMADS26 is mainly expressed in reproductive organs including flowers and fruits. Transgenic Arabidopsis plants with ectopic expression of CsMADS26 exhibited curled rosette and cauline leaves, and the leaf size was much smaller than that of wild-type (WT) plants. These results provide clues for the functional characterization of CsMADS26 in the future.

scholarly journals Pitfall Flower Development and Organ Identity of Ceropegia sandersonii (Apocynaceae-Asclepiadoideae)

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1767
Author(s):  
Annemarie Heiduk ◽  
Dewi Pramanik ◽  
Marlies Spaans ◽  
Loes Gast ◽  
Nemi Dorst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Floral Anatomy ◽  
Floral Organ ◽  
Mads Box ◽  
Floral Organs ◽  
Abcde Model ◽  
Organ Identity ◽  
Mads Box Gene ◽  
Flower Evolution ◽  
Developmental Phases

Deceptive Ceropegia pitfall flowers are an outstanding example of synorganized morphological complexity. Floral organs functionally synergise to trap fly-pollinators inside the fused corolla. Successful pollination requires precise positioning of flies headfirst into cavities at the gynostegium. These cavities are formed by the corona, a specialized organ of corolline and/or staminal origin. The interplay of floral organs to achieve pollination is well studied but their evolutionary origin is still unclear. We aimed to obtain more insight in the homology of the corona and therefore investigated floral anatomy, ontogeny, vascularization, and differential MADS-box gene expression in Ceropegia sandersonii using X-ray microtomography, Light and Scanning Electronic Microscopy, and RT-PCR. During 10 defined developmental phases, the corona appears in phase 7 at the base of the stamens and was not found to be vascularized. A floral reference transcriptome was generated and 14 MADS-box gene homologs, representing all major MADS-box gene classes, were identified. B- and C-class gene expression was found in mature coronas. Our results indicate staminal origin of the corona, and we propose a first ABCDE-model for floral organ identity in Ceropegia to lay the foundation for a better understanding of the molecular background of pitfall flower evolution in Apocynaceae.

scholarly journals GmAGL1, a MADS-Box Gene from Soybean, Is Involved in Floral Organ Identity and Fruit Dehiscence

2017 ◽  
Vol 8 ◽  
Author(s):  
Yingjun Chi ◽  
Tingting Wang ◽  
Guangli Xu ◽  
Hui Yang ◽  
Xuanrui Zeng ◽  
...  
Keyword(s):  
Floral Organ ◽  
Mads Box ◽  
Floral Organ Identity ◽  
Organ Identity ◽  
Mads Box Gene ◽  
Fruit Dehiscence

scholarly journals MADS-box genes are involved in floral development and evolution.

2001 ◽  
Vol 48 (2) ◽  
pp. 351-358 ◽  
Author(s):  
H Saedler ◽  
A Becker ◽  
K U Winter ◽  
C Kirchner ◽  
G Theissen
Keyword(s):  
Floral Development ◽  
Higher Plants ◽  
Control Cell ◽  
Floral Organ ◽  
Reproductive Organs ◽  
Mads Box ◽  
Mads Box Genes ◽  
Flower Bud ◽  
Organ Identity ◽  
Eukaryotic Organisms

MADS-box genes encode transcription factors in all eukaryotic organisms thus far studied. Plant MADS-box proteins contain a DNA-binding (M), an intervening (I), a Keratin-like (K) and a C-terminal C-domain, thus plant MADS-box proteins are of the MIKC type. In higher plants most of the well-characterized genes are involved in floral development. They control the transition from vegetative to generative growth and determine inflorescence meristem identity. They specify floral organ identity as outlined in the ABC model of floral development. Moreover, in Antirrhinum majus the MADS-box gene products DEF/GLO and PLE control cell proliferation in the developing flower bud. In this species the DEF/GLO and the SQUA proteins form a ternary complex which determines the overall "Bauplan" of the flower. Phylogenetic reconstructions of MADS-box sequences obtained from ferns, gymnosperms and higher eudicots reveal that, although ferns possess already MIKC type genes, these are not orthologous to the well characterized MADS-box genes from gymnosperms or angiosperms. Putative orthologs of floral homeotic B- and C-function genes have been identified in different gymnosperms suggesting that these genes evolved some 300-400 million years ago. Both gymnosperms and angiosperms also contain a hitherto unknown sister clade of the B-genes, which we termed Bsister. A novel hypothesis will be described suggesting that B and Bsister might be involved in sex determination of male and female reproductive organs, respectively.

Ectopic expression of a Eucalyptus grandis SVP orthologue alters the flowering time of Arabidopsis thaliana

2004 ◽  
Vol 31 (3) ◽  
pp. 217 ◽  
Author(s):  
Elizabeth M. Brill ◽  
John M. Watson
Keyword(s):  
Arabidopsis Thaliana ◽  
Sequence Data ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Eucalyptus Grandis ◽  
Floral Organ ◽  
Sequence Alignments ◽  
Nucleotide Sequence Data ◽  
Mads Box Gene ◽  
Nucleotide Database

A new MADS-box gene, EgrSVP was isolated from vegetative tips of Eucalyptus grandis Hill ex Maiden saplings. This gene was expressed in vegetative tissues such as shoots, leaves and roots, as well as in unopened floral buds. DNA sequence alignments indicate that EgrSVP shares the highest level of sequence identity with PkMADS1, JOINTLESS, IbMADS3 and SVP. Phylogenetically, it is grouped in the JOINTLESS clade, the members of which share similar expression patterns. Transgenic Arabidopsis thaliana (L.) Heynh. plants overexpressing EgrSVP, exhibited a variety of altered phenotypes, including homeotic floral organ transformation, indeterminate floral development, multiple inflorescences and coflorescences, and some degree of late flowering.The nucleotide sequence data reported will appear in the GenBank Nucleotide Database under the accession number AY263809.

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