scholarly journals Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study

Healthcare ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 514
Author(s):  
Benedetta Baldari ◽  
Simona Vittorio ◽  
Francesco Sessa ◽  
Luigi Cipolloni ◽  
Giuseppe Bertozzi ◽  
...  
Keyword(s):  
Experimental Study ◽  
Immunohistochemical Staining ◽  
Soft Tissues ◽  
Bone Fractures ◽  
Glycophorin A ◽  
Forensic Application ◽  
Radiological Investigation ◽  
Fracture Region ◽  
Three Samples ◽  
Human Glycophorin

Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence in tissues. This experimental study aims to investigate anti-GPA immunohistochemical staining in order to evaluate the vitality of the lesion from corpses in different decomposition state. Six cases were selected, analyzing autopsies’ documentation performed by the Institute of Legal Medicine of Rome in 2010–2018: four samples of fractured bones and three samples of soft tissues. For the control case, the fracture region of the femur was sampled. The results of the present study confirm the preliminary results of other studies, remarking the importance of the GPA immunohistochemical staining to highlight signs of survival. Moreover, this study suggests that the use of this technique should be routinely applied in cases of corpses with advanced putrefaction phenomena, even when the radiological investigation is performed, the macroscopic investigation is inconclusive, the H&E staining is not reliable. This experimental application demonstrated that the use of monoclonal antibody anti-human GPA on bone fractures and soft tissues could be important to verify whether the lesion is vital or not.

scholarly journals Structural variation of the malaria-associated human glycophorin A-B-E region

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Sandra Louzada ◽  
Walid Algady ◽  
Eleanor Weyell ◽  
Luciana W. Zuccherato ◽  
Paulina Brajer ◽  
...  
Keyword(s):  
Structural Variation ◽  
Glycophorin A ◽  
E Region ◽  
Human Glycophorin

Fibrinolysis After Multiple Trauma In Man. Clinical And Methodological Aspects

1981 ◽  
Author(s):  
A-C Teger-Nilsson ◽  
E Gyzander ◽  
A Medegård ◽  
B Risberq ◽  
B Wiman
Keyword(s):  
Traffic Accidents ◽  
Soft Tissues ◽  
Bone Fractures ◽  
Degradation Products ◽  
Clinical Situation ◽  
Fibrinolytic System ◽  
Phase Reaction ◽  
Chromogenic Substrate ◽  
Immunological Method ◽  
Activity Measurements

Fifteen patients with at least two bone fractures and damages of soft tissues, mainly after traffic accidents, were investigated for changes in the fibrinolytic system. Seventeen healthy students were controls. The patients were conventionally treated in an intensive care unit. Blood samples, in all 140, were taken on the day of admission and then daily for about one week. The blood was analysed for plasminogen with electroimmunoassay and with chromogenic substrate after streptokinase activation, for α 2-antiplasmin with electroimmunoassay and two types of activity measurements (chromogenic substrate), for α 2-antiplasmin - plasmin complexes with RIA technique, and for α 2-macroglobulin with electroimmunoassay and activity measurements. In addition fibrinogen, fibrinogen-fibrin degradation products and fibrinolytic activity on fibrin plates were estimated.Plasminogen was somewhat low the first days after trauma lowest 69 % ± 22 % (M ± SD), day 3]. It then slowly increased towards the end of the investigation (highest 123% ± 21%). The last days of investigation the immunological method also gave higher values than the activity method, α 2 Antiplasmin was low directly after the trauma (lowest 67% ± 18%, day 1 ) and increased gradually (highest 115% ± 19%, day 6 ). The first days after trauma immunological method gave higher values than the activity methods, α 2 Antiplasmin - plasmin complexes varied, but showed occasionally high values in particular the first day. α 2 -Macroglobulin decreased only slightly on day 5-6, when it also showed a difference between the immunological and activity methods.It is concluded that the fibrinolytic system is activated immediately after a trauma. Later there is an increase of plasminogen, α 2 antiplasmin and fibrinogen, probably due to an acute phase reaction. Some reasons for the methodological discrepancies are discussed. Serial analysis of α 2-antiplasmin activity seems to be a relevant method for evaluation of fibrinolysis in a complex clinical situation.

An Experimental Study of Pictures Produced by Acute Schizophrenic Subjects

1979 ◽  
Vol 134 (2) ◽  
pp. 195-200 ◽  
Author(s):  
Stephen Russell-Lacy ◽  
Victoria Robinson ◽  
Janet Benson ◽  
Janet Cranage
Keyword(s):  
Experimental Study ◽  
Differential Diagnosis ◽  
Psychiatric Diagnosis ◽  
Psychiatric Patients ◽  
Psychiatric Admission ◽  
Three Samples ◽  
Colour Variety

SummaryThe purpose of this study was to examine the validity of assessing pictures produced by acute schizophrenic subjects as a technique in differential diagnosis. It was hypothesized that art productions by schizophrenics differ from those both by other acute psychiatric patients and by ‘normals'. Coloured slides were made from pictures produced in standardized conditions by three samples each of thirty subjects. It was found that repetition of abstract forms was the only factor studied to be associated specifically with schizophrenia; whereas the presence of pictorial imbalance, overelaboration, childlike features, uncovered space, detail and colour variety were found to be associated with psychiatric admission irregardless of diagnosis. Doubt is cast on the use of art as a technique in differential psychiatric diagnosis.

scholarly journals The mechanism of production of multiple mRNAs for human glycophorin A

1990 ◽  
Vol 18 (19) ◽  
pp. 5829-5836 ◽  
Author(s):  
Jawed Hamid ◽  
Alfred T.H. Burness
Keyword(s):  
Glycophorin A ◽  
Human Glycophorin

SEDIMENT PERMEABILITY AND THE PRESERVATION OF SOFT-TISSUES IN CONCRETIONS: AN EXPERIMENTAL STUDY

Palaios ◽  
2015 ◽  
Vol 30 (8) ◽  
pp. 608-612 ◽  
Author(s):  
VICTORIA E. MCCOY ◽  
ROBERT T. YOUNG ◽  
DEREK E.G. BRIGGS
Keyword(s):  
Experimental Study ◽  
Soft Tissues ◽  
Sediment Permeability

scholarly journals A Rare Case of Myeloid Sarcoma Presenting as an Anorectal Ulcer

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Laxmi Parsa ◽  
Priti Bijpuria ◽  
Daniel Ringold ◽  
David Stein
Keyword(s):  
Immunohistochemical Staining ◽  
Myeloid Leukemia ◽  
Rare Case ◽  
Soft Tissues ◽  
Histochemical Staining ◽  
Myeloid Sarcoma ◽  
Atypical Presentation ◽  
Rare Tumor ◽  
Gastrointestinal Complaint ◽  
Acute Myeloid

Myeloid Sarcoma is a rare tumor composed of myeloblasts occurring at an extramedullary site like bones, or various soft tissues. Myeloid sarcoma may involve the gastrointestinal tract very rarely either solitarily, or occurring simultaneously with acute myeloid leukemia. Its diagnosis is challenging and needs biopsy and immunohistochemical staining. We are describing a case of myeloid sarcoma which presented as a painful anal ulcer mimicking an atypical fissure. Its appearance resembled crohn’s disease on sigmoidoscopy. A biopsy of the ulcer along with histochemical staining led to the diagnosis of myeloid sarcoma. Our case demonstrates the need for aggressive evaluation of any common gastrointestinal complaint with an atypical presentation.

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