scholarly journals Metabolic Functional Community Diversity of Associated Bacteria during the Degradation of Phytoplankton from a Drinking Water Reservoir

Author(s):  
Sheng-Nan Chen ◽  
Pan-Lu Shang ◽  
Peng-Liang Kang ◽  
Man-Man Du

In the drinking water reservoir ecosystem, phytoplankton and bacteria play important roles in shaping freshwater health and function. In this work, the associated bacterial community functional diversity during degradation of phytoplankton was determined using the substrate utilization profiling (BIOLOG) technique, meanwhile, the composition and concentration of phytoplankton were examined using a microscope. The results indicated that Euglena decreased 58.33% from 0 to 38 d, while the smallest degradation of Bacillariophyta was 20.19%. Average well color development (AWCD590nm) increased during the static periods from 0 to 38 d; however, the AWCD590nm of 18 and 38 d had no significant difference (p < 0.05). The Simpson’s index (D) was in accordance with Shannon’s diversity (H) and species richness(S); it was measured to be18 > 38 > 5 > 0 d. There were significant differences in the pattern and level of carbon sources used by the phytoplankton-associated bacteria. In addition, the principle component analyses (PCA) suggested that the first principle component (PC1) and the second principle component (PC2) explained 46.76% and 21.49% of the total variation for bacterial community, respectively. Redundancy analysis (RDA) revealed that cell abundance of phytoplankton was negatively correlated with the AWCD590nm, amino acids and other functional indexes. Therefore, the data suggest that there are differences in the phytoplankton-associated bacterial community functional diversity during different static stages of water samples collected from the drinking water reservoir.

Author(s):  
Zizhen Zhou ◽  
Tinlin Huang ◽  
Weijin Gong ◽  
Yang Li ◽  
Yue Liu ◽  
...  

Field research on the performance of pollutant removal and the structure of the microbial community was carried out on a drinking water reservoir. After one month of operation of a water-lifting aeration system, the water temperature difference between the bottom and the surface decreased from 9.9 to 3.1 °C, and the concentration of the dissolved oxygen (DO) in the bottom layer increased from 0 to 4.2 mg/L. The existing stratification in the reservoir was successfully eliminated. Total nitrogen (TN), total phosphorus (TP), and total organic carbon (TOC) concentrations were reduced by 47.8%, 66.7%, and 22.9%, respectively. High-throughput sequencing showed that Proteobacteria, Bacteroides, and Actinomycetes accounted for 67.52% to 78.74% of the total bacterial population. Differences in the bacterial changes were observed between the enhanced area and the control area. With the operation of the water-lifting aeration system, the populations of bacteria of the main genera varied temporally and spatially. Principal component analysis pointed out a clear evolution in the vertical distribution of the microbial structure controlled by the operation of the aeration system. Permutational analysis of variance showed a significant difference in the microbial community (p < 0.01). Redundancy analysis showed that physical (water temperature, DO) and chemical environmental factors (Chl-a, TOC, TN) were the key factors affecting the changes in the microbial communities in the reservoir water. In addition, a hierarchical partitioning analysis indicated that T, Chl-a, ORP, TOC, pH, and DO accounted for 24.1%, 8.7%, 6.7%, 6.2%, 5.8%, and 5.1% of such changes, respectively. These results are consistent with the ABT (aggregated boosted tree) analysis for the variations in the functional bacterial community, and provide a theoretical basis for the development and application of biotechnology.


2021 ◽  
Vol 596 ◽  
pp. 126090
Author(s):  
Haihan Zhang ◽  
Kaiwen Liu ◽  
Tinglin Huang ◽  
Nan Li ◽  
Fan Si ◽  
...  

2017 ◽  
Vol 2017 (6) ◽  
pp. 4586-4604
Author(s):  
Ari Elden ◽  
Gary Skipper ◽  
Don Gordon ◽  
Ernesto Fernandez ◽  
Chris Garrett

2021 ◽  
Vol 192 ◽  
pp. 116848
Author(s):  
Ming Su ◽  
Yiping Zhu ◽  
Zeyu Jia ◽  
Tingting Liu ◽  
Jianwei Yu ◽  
...  

2021 ◽  
pp. 117069
Author(s):  
Allan A. Santos ◽  
Dayvson O. Guedes ◽  
Mário U.G. Barros ◽  
Samylla Oliveira ◽  
Ana B.F. Pacheco ◽  
...  

2008 ◽  
Vol 13 (4) ◽  
pp. 289-300 ◽  
Author(s):  
Tiia Pedusaar ◽  
Ilkka Sammalkorpi ◽  
Arto Hautala ◽  
Ain Järvalt

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