The Cytoprotective Effect of Sulfuretin against  tert-Butyl Hydroperoxide-Induced Hepatotoxicity  through Nrf2/ARE and JNK/ERK MAPK-Mediated  Heme Oxygenase-1 Expression

Sargassum species have been reported to be a source of phytochemicals, with a wide range of biological activities. In this study, we evaluated the hepatoprotective effect of a meroterpenoid-rich fraction of the ethanolic extract from Sargassum serratifolium (MES) against tert-butyl hydroperoxide (t-BHP)-treated HepG2 cells. Treatment with MES recovered the cell viability from the t-BHP-induced oxidative damage in a dose-dependent manner. It suppressed the reactive oxygen species production, lipid peroxidation, and glutathione depletion in the t-BHP-treated HepG2 cells. The activity of the antioxidants induced by t-BHP, including superoxide dismutase (SOD) and catalase, was reduced by the MES treatment. Moreover, it increased the nuclear translocation of nuclear factor erythroid 2-related factor 2, leading to the enhanced activity of glutathione S transferase, and the increased production of heme oxygenase-1 and NAD(P)H:quinine oxidoreductase 1 in t-BHP-treated HepG2 cells. These results demonstrate that the antioxidant activity of MES substituted the activity of the SOD and catalase, and induced the production of detoxifying enzymes, indicating that MES might be used as a hepatoprotectant against t-BHP-induced oxidative stress.

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Total Flavonoids of Crocus sativus Petals Release tert-Butyl Hydroperoxide-Induced Oxidative Stress in BRL-3A Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/5453047 ◽

2021 ◽

Vol 2021 ◽

pp. 1-15

Author(s):

Hong Ye ◽

Juan Luo ◽

Dongmei Hu ◽

Shuting Yang ◽

Aolai Zhang ◽

...

Keyword(s):

Superoxide Dismutase ◽

Antioxidant Capacity ◽

B Cell Lymphoma ◽

Crocus Sativus ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Nuclear Factor ◽

Butyl Hydroperoxide ◽

Tert Butyl ◽

Tert Butyl Hydroperoxide

Antioxidant and hepatoprotective activities in vitro of saffron petals were examined in this study for better utilizing saffron (Crocus sativus L.) biowaste. Using the DPPH and ABTS radical scavenging method, we compared the antioxidant activity and the content of total flavonoid extracts from petals (TFESP), stamens (TFESS), and both saffron petals and stamens (TFEMS). The results showed that the antioxidant capacity and the flavonoid content of TFESP were higher than those of TFESS and TFEMS. Then, the hepatoprotective activity of TFESP was determined, and the silymarin was used as a positive control. The main components of TFESP were analysed by ultrahigh performance liquid chromatography (UPLC) photodiode array (PDA)/mass spectrometry (MS) and nuclear magnetic resonance (NMR). The result showed that (1) TFESP could release oxidative liver injury induced by tert-butyl hydroperoxide (t-BHP). (2) TFESP could reduce the accumulation of reactive oxygen species (ROS); enhance the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH); and then improve the total antioxidant capacity (T-AOC) in BRL-3A cells. (3) TFESP could enhance the expression of B-cell lymphoma-2 (BCL-2) and decrease the expression of caspase-3 and caspase-9; increase the expression of Kelch-like ECH-associated protein-1 (Keap-1), nuclear factor, erythroid 2-related factor 2 (Nrf2), superoxide dismutase, and heme oxygenase 1 (HO-1); and downregulate inducible nitric oxide synthase (INOS), interleukin-6 (IL-6), and nuclear factor kappa B-9 (NF-κB-9). (4) The main hepatoprotective component of TFESP was identified as kaempferol-3-o-sophoroside. The mechanism may be that kaempferol-3-o-sophoroside can protect t-BHP-induced cell injury by regulating the expression of antioxidant, antiapoptotic, and anti-inflammatory genes. Thus, saffron petals are a potential hepatoprotective resource worthy of development.

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Chrysin and rutin protect against hydrogen peroxide and tert-butyl hydroperoxide induced oxidative cell damage

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i6.5131 ◽

2021 ◽

Vol 11 (6) ◽

pp. 20-25

Author(s):

Rouamba Ablassé ◽

Compaoré Moussa ◽

Ouédraogo Maurice ◽

Bationo Raoul ◽

Kiendrebeogo Martin

Keyword(s):

Hydrogen Peroxide ◽

Cell Damage ◽

Human Gingival Fibroblasts ◽

Dependent Manner ◽

Gingival Fibroblasts ◽

Cytoprotective Effect ◽

Butyl Hydroperoxide ◽

Tert Butyl ◽

Thiazolyl Blue Tetrazolium Bromide ◽

Tert Butyl Hydroperoxide

Objective: Chrysin and rutin are two dietary flavonoids lying in fruits or honey bee’s products. Their pharmacological properties include antioxidant, anti-inflammatory, anticancer, neuroprotection and immunomodulatory. In the current study, the potentiality of chrysin and rutin to protect human gingival fibroblasts against oxidative cell damage has been investigated in vitro. Method: Human gingival fibroblasts, passage 3, were concomitantly put in contact with the cytotoxic compounds and chrysin or rutin for 24 h at 37 °C, 5% CO2 atmosphere, and 96% humidity. The amount of viable cell after the incubated time was recorded by using the thiazolyl blue tetrazolium bromide (MTT) assay. Results: Chrysin in all tested concentration didn’t exhibit any cytoprotective effect against the tert-butyl hydroperoxide-induced oxidative cell damage. Moreover, chrysin in a low concentration (5 and 10 µg/mL) didn’t protect the fibroblasts against oxidative cell damage induced by the hydrogen peroxide. However, chrysin in a concentration of 20 µg/mL showed a significant cytoprotective activity in the hydrogen peroxide-induced cell damage (p < 0.05). Rutin in all tested concentrations protected fibroblasts against hydrogen peroxide and tert-butyl hydroperoxide-induced oxidative cell damage. The cytoprotective effect of rutin didn’t increase with the increase of the concentration when hydrogen peroxide is used to induce oxidative cell damage. However, rutin has protected cells against the tert-butyl hydroperoxide cytotoxicity in a concentration dependent manner. Conclusion: Given to the interesting cytoprotective activities exhibited by chrysin and rutin, further investigations to highlight their cytoprotective involved mechanisms are justified. Keywords: Chrysin, Cytoprotective, Fibroblasts, Rutin.

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