scholarly journals The Relevance of Mass Spectrometry Analysis for Personalized Medicine through Its Successful Application in Cancer “Omics”

2019 ◽  
Vol 20 (10) ◽  
pp. 2576 ◽  
Author(s):  
Cristina Alexandra Ciocan-Cartita ◽  
Ancuța Jurj ◽  
Mihail Buse ◽  
Diana Gulei ◽  
Cornelia Braicu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Biologically Active ◽  
Mass Spectrometry Analysis ◽  
Active Metabolites ◽  
Spectrometry Analysis ◽  
Bodily Fluids ◽  
New Biomarkers ◽  
Biologically Active Metabolites ◽  
Analytical Technology

Mass spectrometry (MS) is an essential analytical technology on which the emerging omics domains; such as genomics; transcriptomics; proteomics and metabolomics; are based. This quantifiable technique allows for the identification of thousands of proteins from cell culture; bodily fluids or tissue using either global or targeted strategies; or detection of biologically active metabolites in ultra amounts. The routine performance of MS technology in the oncological field provides a better understanding of human diseases in terms of pathophysiology; prevention; diagnosis and treatment; as well as development of new biomarkers; drugs targets and therapies. In this review; we argue that the recent; successful advances in MS technologies towards cancer omics studies provides a strong rationale for its implementation in biomedicine as a whole.

scholarly journals Primary Structure and Antibacterial Activity of Chicken Bone Marrow-Derived β-Defensins

2009 ◽  
Vol 53 (11) ◽  
pp. 4647-4655 ◽  
Author(s):  
Chrystelle Derache ◽  
Valérie Labas ◽  
Vincent Aucagne ◽  
Hervé Meudal ◽  
Céline Landon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bone Marrow ◽  
Posttranslational Modifications ◽  
Amino Acid Sequences ◽  
Biologically Active ◽  
Mass Spectrometry Analysis ◽  
Gram Positive ◽  
Intact Cells ◽  
Spectrometry Analysis ◽  
Chicken Bone

ABSTRACTThree biologically active β-defensins were purified by chromatography from chicken bone marrow extract: avian β-defensin 1 (AvBD1), AvBD2, and the newly isolated β-defensin AvBD7. Mass spectrometry analyses showed that bone marrow-derived AvBD1, -2, and -7 peptides were present as mature peptides and revealed posttranslational modifications for AvBD1 and AvBD7 in comparison to their in silico-predicted amino acid sequences. Tandem mass spectrometry analysis using the nanoelectrospray-quadrupole time of flight method showed N-terminal glutaminyl cyclization of mature AvBD7 and C-terminal amidation of mature AvBD1 peptide, while posttranslational modifications were absent in bone marrow-derived mature AvBD2 peptide. Furthermore, mass spectrometry analysis performed on intact cells confirmed the presence of these three peptides in mature heterophils. In addition, the antibacterial activities of the three β-defensins against a large panel of gram-positive and -negative bacteria were assessed. While the three defensins displayed similar antibacterial spectra of activity against gram-positive strains, AvBD1 and AvBD7 exhibited the strongest activity against gram-negative strains in comparison to AvBD2.

scholarly journals HPLC and mass spectrometry analysis of dolichol-phosphates at the cell culture scale

2010 ◽  
Vol 396 (1) ◽  
pp. 133-138 ◽  
Author(s):  
Micha A. Haeuptle ◽  
Andreas J. Hülsmeier ◽  
Thierry Hennet
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis

Current Progress of Lipid Analysis in Metabolic Diseases by Mass Spectrometry Methods

2019 ◽  
Vol 26 (1) ◽  
pp. 60-103 ◽  
Author(s):  
Adriana Mika ◽  
Tomasz Sledzinski ◽  
Piotr Stepnowski
Keyword(s):  
Mass Spectrometry ◽  
Metabolic Diseases ◽  
Lipid Analysis ◽  
Mass Spectrometry Analysis ◽  
Human Organism ◽  
Aliphatic Chain ◽  
Lipid Profiling ◽  
Multivariate Statistical ◽  
Spectrometry Analysis ◽  
New Biomarkers

Background: Obesity, insulin resistance, diabetes, and metabolic syndrome are associated with lipid alterations, and they affect the risk of long-term cardiovascular disease. A reliable analytical instrument to detect changes in the composition or structures of lipids and the tools allowing to connect changes in a specific group of lipids with a specific disease and its progress, is constantly lacking. Lipidomics is a new field of medicine based on the research and identification of lipids and lipid metabolites present in human organism. The primary aim of lipidomics is to search for new biomarkers of different diseases, mainly civilization diseases. Objective: We aimed to review studies reporting the application of mass spectrometry for lipid analysis in metabolic diseases. Method: Following an extensive search of peer-reviewed articles on the mass spectrometry analysis of lipids the literature has been discussed in this review article. Results: The lipid group contains around 1.7 million species; they are totally different, in terms of the length of aliphatic chain, amount of rings, additional functional groups. Some of them are so complex that their complex analyses are a challenge for analysts. Their qualitative and quantitative analysis of is based mainly on mass spectrometry. Conclusion: Mass spectrometry techniques are excellent tools for lipid profiling in complex biological samples and the combination with multivariate statistical analysis enables the identification of potential diagnostic biomarkers.

scholarly journals Viridistratins A−C, Antimicrobial and Cytotoxic Benzo[j]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota)

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 805 ◽  
Author(s):  
Kevin Becker ◽  
Anna-Charleen Wessel ◽  
J. Jennifer Luangsa-ard ◽  
Marc Stadler
Keyword(s):  
Mass Spectrometry ◽  
High Resolution Mass Spectrometry ◽  
Biologically Active ◽  
Cytotoxic Activities ◽  
Tropical Fungi ◽  
Active Metabolites ◽  
Chemical Structures ◽  
New Compounds ◽  
Biologically Active Metabolites ◽  
Resolution Mass

During the course of our search for novel biologically active metabolites from tropical fungi, we are using chemotaxonomic and taxonomic methodology for the preselection of interesting materials. Recently, three previously undescribed benzo[j]fluoranthenes (1−3) together with the known derivatives truncatones A and C (4, 5) were isolated from the stromata of the recently described species Annulohypoxylon viridistratum collected in Thailand. Their chemical structures were elucidated by means of spectral methods, including nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). The new compounds, for which we propose the trivial names viridistratins A−C, exhibited weak-to-moderate antimicrobial and cytotoxic activities in cell-based assays.

Simple Synthesis of Fluorinated Ene-Ynes via In Situ Generation of Allenes

Synthesis ◽  
2021 ◽  
Author(s):  
Ellen M. Sletten ◽  
Joseph A. Jaye
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Biologically Active ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Synthetic Route ◽  
Spectrometry Analysis ◽  
Complex Products ◽  
In Situ Generation

AbstractFluorination of small molecules is a key route toward modulating reactivity and bioactivity. The 1,3 ene-yne functionality is an important synthon towards complex products, as well as a common functionality in biologically active molecules. Here, we present a new synthetic route towards fluorinated ene-ynes from simple starting materials. We employ gas chromatography-mass spectrometry analysis to probe the sequential eliminations necessary for this transformation and observe an allene intermediate. The ene-yne products are sufficiently fluorous to enable purification via fluorous extraction. This methodology will allow facile access to functional, fluorous ene-ynes.

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