scholarly journals 6-SFT, a Protein from Leymus mollis, Positively Regulates Salinity Tolerance and Enhances Fructan Levels in Arabidopsis thaliana

2019 ◽  
Vol 20 (11) ◽  
pp. 2691
Author(s):  
Mao Li ◽  
Xiaolan He ◽  
Dongdong Hao ◽  
Jun Wu ◽  
Jixin Zhao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Primary Root ◽  
Salt Stress Tolerance ◽  
Primary Root Growth ◽  
Rapid Amplification ◽  
Leymus Mollis

Fructans play vital roles in abiotic stress tolerance in plants. In this study, we isolated the sucrose:6-fructosyltransferase gene, which is involved in the synthesis of fructans, from Leymus mollis by rapid amplification of cDNA ends. The Lm-6-SFT gene was introduced into Arabidopsis thaliana cv. Columbia by Agrobacterium-mediated transformation. The transgenic plants were evaluated under salt stress conditions. The results showed that the expression of Lm-6-SFT was significantly induced by light, abscisic acid (ABA), salicylic acid (SA), and salt treatment in L. mollis plants. Overexpression of Lm-6-SFT in Arabidopsis promoted seed germination and primary root growth during the early vegetative growth stage under salt stress. We also found that the transgenic plants expressing Lm-6-SFT had increased proline and fructan levels. β-Glucuronidase staining and promoter analysis indicated that the promoter of Lm-6-SFT was regulated by light, ABA, and salt stress. Quantitative PCR suggested that overexpression of Lm-6-SFT could improve salt tolerance by interacting with the expression of some salt stress tolerance genes. Thus, we demonstrated that the Lm-6-SFT gene is a candidate gene that potentially confers salt stress tolerance to plants. Our study will aid the elucidation of the regulatory mechanism of 6-SFT genes in herb plants.

scholarly journals Accession-specific Parent-of-origin Dependent and Independent Genome Dosage Effects on Salt Tolerance in Arabidopsis Thaliana

2022 ◽  
Author(s):  
Brendan F. Hallahan ◽  
Galina Brychkova ◽  
Peter McKeown ◽  
Charles Spillane
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Salt Stress Tolerance ◽  
Metabolic Characteristics ◽  
Dosage Effects ◽  
Novel Traits ◽  
Parent Of Origin

Abstract Improving the salt stress tolerance of crops is an important goal in plant breeding. Changes in the number of chromosome pairs (i.e. ploidy level) cause genome dosage effects which can result in improved traits or emergence of novel traits. The genetic and epigenetic contribution of maternal or paternal chromosomes can differentially affect physiological and metabolic characteristics of F1 offspring. Hence genome dosage effects can be parent-of-origin independent or dependent. The model plant Arabidopsis thaliana displays both genome dosage and parent-of-origin effects on plant growth under normal, non-stress conditions. Using an insogenic ploidy series of diploid, triploid and tetraploid lines we investigate the extent of genome dosage effects and their parent-of-origin dependency on in vitro salt stress tolerance of seedlings across ten different A. thaliana accessions (genetic backgrounds). We demonstrate genome dosage effects on salt stress tolerance in five accessions, and using reciprocal triploid lines demonstrate parent-of-origin dependent genome dosage effects on salt stress tolerance in three accessions. Our results indicate that epigenetic genome dosage and genome dosage balance effects can have significant impacts on abiotic stress tolerance in plants.

scholarly journals Transcriptome Changes Reveal the Molecular Mechanisms of Humic Acid-Induced Salt Stress Tolerance in Arabidopsis

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 782
Author(s):  
Joon-Yung Cha ◽  
Sang-Ho Kang ◽  
Myung Geun Ji ◽  
Gyeong-Im Shin ◽  
Song Yi Jeong ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Humic Acid ◽  
Stress Tolerance ◽  
Plant Development ◽  
Molecular Mechanisms ◽  
Abiotic Stress Tolerance ◽  
Principal Component ◽  
Salt Stress Tolerance ◽  
Genes Encoding

Humic acid (HA) is a principal component of humic substances, which make up the complex organic matter that broadly exists in soil environments. HA promotes plant development as well as stress tolerance, however the precise molecular mechanism for these is little known. Here we conducted transcriptome analysis to elucidate the molecular mechanisms by which HA enhances salt stress tolerance. Gene Ontology Enrichment Analysis pointed to the involvement of diverse abiotic stress-related genes encoding HEAT-SHOCK PROTEINs and redox proteins, which were up-regulated by HA regardless of salt stress. Genes related to biotic stress and secondary metabolic process were mainly down-regulated by HA. In addition, HA up-regulated genes encoding transcription factors (TFs) involved in plant development as well as abiotic stress tolerance, and down-regulated TF genes involved in secondary metabolic processes. Our transcriptome information provided here provides molecular evidences and improves our understanding of how HA confers tolerance to salinity stress in plants.

scholarly journals Overexpression of a Novel Component Induces HAK5 and Enhances Growth in Arabidopsis

ISRN Botany ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Eri Adams ◽  
Celine Diaz ◽  
Minami Matsui ◽  
Ryoung Shin
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Adaptive Response ◽  
Screening Method ◽  
Primary Root ◽  
Nutrient Deficiency ◽  
Potassium Deficiency ◽  
Low Potassium ◽  
Primary Root Growth ◽  
Gene Hunting

Plants have developed mechanisms to adapt to the potassium deficient conditions over the years. In Arabidopsis thaliana, expression of a potassium transporter HAK5 is induced in low potassium conditions as an adaptive response to nutrient deficiency. In order to understand the mechanism in which HAK5 is regulated, the full-length cDNA overexpressor gene hunting system was employed as a screening method. Of 40 genes recovered, At4g18280 was found to be dramatically induced in response to potassium-deficiency and salt stress. Plants overexpressing this gene showed higher HAK5 expression and enhanced growth. These plants were also less sensitive to potassium-deficiency in terms of primary root growth. Taken together, these data suggest that this novel component, At4g18280, contributes to regulation of HAK5 and, consequently, tolerance to potassium-deficiency in plants.

scholarly journals Overexpression of a S-Adenosylmethionine Decarboxylase from Sugar Beet M14 Increased Araidopsis Salt Tolerance

2019 ◽  
Vol 20 (8) ◽  
pp. 1990 ◽  
Author(s):  
Meichao Ji ◽  
Kun Wang ◽  
Lin Wang ◽  
Sixue Chen ◽  
Haiying Li ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Sugar Beet ◽  
Stress Tolerance ◽  
Membrane Damage ◽  
Pcr Analysis ◽  
Ros Generation ◽  
Salt Stress Tolerance ◽  
Adenosylmethionine Decarboxylase

Polyamines play an important role in plant growth and development, and response to abiotic stresses. Previously, differentially expressed proteins in sugar beet M14 (BvM14) under salt stress were identified by iTRAQ-based quantitative proteomics. One of the proteins was an S-adenosylmethionine decarboxylase (SAMDC), a key rate-limiting enzyme involved in the biosynthesis of polyamines. In this study, the BvM14-SAMDC gene was cloned from the sugar beet M14. The full-length BvM14-SAMDC was 1960 bp, and its ORF contained 1119 bp encoding the SAMDC of 372 amino acids. In addition, we expressed the coding sequence of BvM14-SAMDC in Escherichia coli and purified the ~40 kD BvM14-SAMDC with high enzymatic activity. Quantitative real-time PCR analysis revealed that the BvM14-SAMDC was up-regulated in the BvM14 roots and leaves under salt stress. To investigate the functions of the BvM14-SAMDC, it was constitutively expressed in Arabidopsis thaliana. The transgenic plants exhibited greater salt stress tolerance, as evidenced by longer root length and higher fresh weight and chlorophyll content than wild type (WT) under salt treatment. The levels of spermidine (Spd) and spermin (Spm) concentrations were increased in the transgenic plants as compared with the WT. Furthermore, the overexpression plants showed higher activities of antioxidant enzymes and decreased cell membrane damage. Compared with WT, they also had low expression levels of RbohD and RbohF, which are involved in reactive oxygen species (ROS) production. Together, these results suggest that the BvM14-SAMDC mediated biosynthesis of Spm and Spd contributes to plant salt stress tolerance through enhancing antioxidant enzymes and decreasing ROS generation.

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