scholarly journals Mildew Resistance Locus O Genes CsMLO1 and CsMLO2 Are Negative Modulators of the Cucumis sativus Defense Response to Corynespora cassiicola

2019 ◽  
Vol 20 (19) ◽  
pp. 4793 ◽  
Author(s):  
Guangchao Yu ◽  
Qiumin Chen ◽  
Xiangyu Wang ◽  
Xiangnan Meng ◽  
Yang Yu ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Defense Response ◽  
Leaf Spot ◽  
Resistance Locus ◽  
Corynespora Cassiicola ◽  
Membrane Expression ◽  
Mildew Resistance ◽  
Pathogenesis Related Proteins ◽  
Pathogenesis Related ◽  
Related Proteins

Corynespora leaf spot caused by Corynespora cassiicola is one of the major diseases in cucumber (Cucumis sativus L.). However, the resistance mechanisms and signals of cucumber to C. cassiicola are unclear. Here, we report that the mildew resistance locus O (MLO) genes, CsMLO1 and CsMLO2, are both negative modulators of the cucumber defense response to C. cassiicola. Subcellular localization analysis showed that CsMLO1 and CsMLO2 are localized in the plasma membrane. Expression analysis indicated that the transcript levels of CsMLO1 and CsMLO2 are linked to the defense response to C. cassiicola. Transient overexpression of either CsMLO1 or CsMLO2 in cucumber cotyledons reduced resistance to C. cassiicola, whereas silencing of either CsMLO1 or CsMLO2 enhanced resistance to C. cassiicola. The relationships of pathogenesis-related proteins, reactive oxygen species (ROS)-associated genes, and abscisic acid (ABA)-related genes to the overexpression and silencing of CsMLO1/CsMLO2 in non-infested cucumber plants were investigated. The results indicated that CsMLO1 mediated resistance against C. cassiicola by regulating the expression of pathogenesis-related proteins and ROS-associated genes, as well as through ABA signaling pathway-associated genes. The CsMLO2-mediated resistance against C. cassiicola primarily involves regulation of the expression of pathogenesis-related proteins. Our findings will guide strategies to enhance the resistance of cucumber to corynespora leaf spot.

scholarly journals Transcriptomic Analysis of Genes Involved in Plant Defense Response to the Cucumber Green Mottle Mosaic Virus Infection

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1064
Author(s):  
Anna Slavokhotova ◽  
Tatyana Korostyleva ◽  
Andrey Shelenkov ◽  
Vitalii Pukhalskiy ◽  
Irina Korottseva ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Signaling Pathways ◽  
Mosaic Virus ◽  
Defense Response ◽  
Cucumis Sativus L ◽  
Pathogenesis Related Proteins ◽  
Pathogenesis Related ◽  
Genes Encoding ◽  
Acid Pathway ◽  
Related Proteins

Plants have evolved a complex multilayered defense system to counteract various invading pathogens during their life cycle. In addition to silencing, considered to be a major molecular defense response against viruses, different signaling pathways activated by phytohormones trigger the expression of secondary metabolites and proteins preventing virus entry and propagation. In this study, we explored the response of cucumber plants to one of the global pathogens, cucumber green mottle mosaic virus (CGMMV), which causes severe symptoms on leaves and fruits. The inbred line of Cucumis sativus L., which is highly susceptible to CGMMV, was chosen for inoculation. Transcriptomes of infected plants at the early and late stages of infection were analyzed in comparison with the corresponding transcriptomes of healthy plants using RNA-seq. The changes in the signaling pathways of ethylene and salicylic and jasmonic acids, as well as the differences in silencing response and expression of pathogenesis-related proteins and transcription factors, were revealed. The results show that silencing was strongly suppressed in infected plants, while the salicylic acid and ethylene signaling pathways were induced. The genes encoding pathogenesis-related proteins and the genes involved in the jasmonic acid pathway changed their expression insignificantly. It was also found that WRKY and NAC were the most sensitive to CGMMV infection among the transcription factors detected.

Pathogenesis Related Proteins in Plant Defense Response

2011 ◽  
pp. 379-403 ◽  
Author(s):  
J. Sudisha ◽  
R. G. Sharathchandra ◽  
K. N. Amruthesh ◽  
Arun Kumar ◽  
H. Shekar Shetty
Keyword(s):  
Plant Defense ◽  
Defense Response ◽  
Plant Defense Response ◽  
Pathogenesis Related Proteins ◽  
Pathogenesis Related ◽  
Related Proteins

Pathogenesis‐related proteins of plants

1991 ◽  
Vol 10 (2) ◽  
pp. 123-150 ◽  
Author(s):  
Huub J.M. Linthorst ◽  
L.C. Van Loon
Keyword(s):  
Pathogenesis Related Proteins ◽  
Pathogenesis Related ◽  
Related Proteins

scholarly journals A Faster and a Stronger Defense Response: One of the Key Elements in Grapevine Explaining Its Lower Level of Susceptibility to Esca?

2013 ◽  
Vol 103 (10) ◽  
pp. 1028-1034 ◽  
Author(s):  
Carole Lambert ◽  
Ian Li Kim Khiook ◽  
Sylvia Lucas ◽  
Nadège Télef-Micouleau ◽  
Jean-Michel Mérillon ◽  
...  
Keyword(s):  
Defense Response ◽  
Defense Mechanisms ◽  
Defense Responses ◽  
Cabernet Sauvignon ◽  
Susceptible Cultivar ◽  
Pathogenesis Related ◽  
Leaf Level ◽  
Plant Pathogen Interaction ◽  
Related Proteins ◽  
Stilbene Compounds

Wood diseases like Esca are among the most damaging afflictions in grapevine. The defense mechanisms in this plant–pathogen interaction are not well understood. As some grapevine cultivars have been observed to be less susceptible to Esca than others, understanding the factors involved in this potentially stronger defense response can be of great interest. To lift part of this veil, we elicited Vitis vinifera plants of two cultivars less susceptible to Esca (‘Merlot’ and ‘Carignan’) and of one susceptible cultivar (‘Cabernet Sauvignon’), and monitored their defense responses at the leaf level. Our model of elicitation consisted in grapevine cuttings absorbing a culture filtrate of one causal agent of Esca, Phaemoniella chlamydospora. This model might reflect the early events occurring in Esca-affected grapevines. The two least susceptible cultivars showed an earlier and stronger defense response than the susceptible one, particularly with regard to induction of the PAL and STS genes, and a higher accumulation of stilbene compounds and some pathogenesis-related proteins.

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