scholarly journals Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site

2020 ◽  
Vol 21 (17) ◽  
pp. 6060 ◽  
Author(s):  
Chiara Luongo ◽  
Leopoldo González-Brusi ◽  
Paula Cots-Rodríguez ◽  
Mª José Izquierdo-Rico ◽  
Manuel Avilés ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Proteome Analysis ◽  
Interacting Proteins ◽  
Sperm Transport ◽  
Two Fluids ◽  
Boar Sperm ◽  
Uterine Fluid ◽  
Proteome Changes ◽  
Oviductal Fluid

Ejaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine fluid, UF; and oviductal fluid, OF) reproductive fluids. The following experimental groups were analyzed: (1) SP: sperm + 20% SP; (2) UF: sperm + 20% UF; (3) OF: sperm + 20% OF; (4) SP + UF: sperm + 20% SP + 20% UF; and (5) SP+OF: sperm + 20% SP + 20% OF. The proteome analysis, performed by HPLC-MS/MS, allowed the identification of 265 proteins. A total of 69 proteins were detected in the UF, SP, and SP + UF groups, and 102 proteins in the OF, SP, and SP + OF groups. Our results showed a higher number of proteins when sperm were incubated with only one fluid than when they were co-incubated with two fluids. Additionally, the number of sperm-interacting proteins from the UF group was lower than the OF group. In conclusion, the interaction of sperm with reproductive fluids alters its proteome. The description of sperm-interacting proteins in porcine species after co-incubation with male and/or female reproductive fluids may be useful to understand sperm transport, selection, capacitation, or fertilization phenomena.

scholarly journals Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1202
Author(s):  
Sergio Navarro-Serna ◽  
Evelyne París-Oller ◽  
Ondrej Simonik ◽  
Raquel Romar ◽  
Joaquín Gadea
Keyword(s):  
Calcium Concentration ◽  
Preparation Method ◽  
Culture Media ◽  
Penetration Rate ◽  
Sperm Parameters ◽  
High Concentration ◽  
Sperm Preparation ◽  
Boar Sperm ◽  
Oviductal Fluid

More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) to in vitro culture media. The objective of this study was to assess the swim-up procedure’s suitability as a sperm selection method using a medium supplemented with 1mg/mL BSA, 1% preovulatory pOF (v/v), 1% v/v pOF plus 1mg/mL BSA, and 5mg/mL BSA. After selection, various sperm parameters were studied, such as sperm recovery rate, sperm morphology, motility (by CASA), vitality, acrosome status and intracellular calcium (by flow cytometry) and ability to penetrate oocytes in vitro. Around 2% of sperm were recovered after swim-up, and the replacement of BSA by pOF showed a beneficial reduction of motility parameters calcium concentration, resulting in an increased penetration rate. The combination of albumin and oviductal fluid in the medium did not improve the sperm parameters results, whereas a high concentration of BSA increased sperm morphological abnormalities, motility, and acrosome damage, with a reduction of calcium concentration and penetration rate. In conclusion, the replacement of albumin by preovulatory oviductal fluid in the swim-up sperm preparation method modifies boar sperm parameters and improves the in vitro penetration of oocytes.

scholarly journals Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing1,2

2016 ◽  
Vol 94 (5) ◽  
pp. 1906-1912 ◽  
Author(s):  
M. A. Torres ◽  
G. M. Ravagnani ◽  
D. F. Leal ◽  
S. M. M. K. Martins ◽  
B. B. D. Muro ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
The Stability

scholarly journals Comparative proteome analysis of blood plasma of patients with early-stage chronic cerebral ischemia

2016 ◽  
Vol 62 (5) ◽  
pp. 599-602 ◽  
Author(s):  
Y.S. Kisrieva ◽  
N.A. Petushkova ◽  
N.F. Samenkova ◽  
G.P. Kuznetsova ◽  
O.V. Larina ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Blood Plasma ◽  
Early Stage ◽  
Proteome Analysis ◽  
Study Group ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chronic Cerebral Ischemia ◽  
Automatic Mode ◽  
Proteome Changes ◽  
Spectrometer Data

In the present study, we explored the technology of liquid chromatography-mass spectrometry (HPLC-MS/MS) for the proteome analysis of blood plasma of patients with early chronic cerebral ischemia. Analysis of mass-spectrometer data carried out in automatic mode using the software Progenesis LS-MS. As a result of this study identified 43 proteins. The differences identified in the study group compared with the control in 7 proteins. It was found that in the early stages of chronic cerebral ischemia proteome changes in blood plasma affect proteins related to the immune system, the system for the maintenance of hemostasis and lipid metabolism.

Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

2018 ◽  
Vol 198 ◽  
pp. 20-26 ◽  
Author(s):  
D.F. Leal ◽  
M.A. Torres ◽  
G.M. Ravagnani ◽  
S.M.M.K. Martins ◽  
F.V. Meirelles ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Quality Characteristics ◽  
Liquid Storage ◽  
Boar Sperm

Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance

2020 ◽  
Vol 157 ◽  
pp. 130-139 ◽  
Author(s):  
Kayla M. Mills ◽  
Uma K. Aryal ◽  
Tiago Sobreira ◽  
Amanda M. Minton ◽  
Theresa Casey ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Reproductive Performance ◽  
Proteome Analysis

scholarly journals Seminal Plasma Cytokines Are Predictive of the Outcome of Boar Sperm Preservation

2019 ◽  
Vol 6 ◽  
Author(s):  
Isabel Barranco ◽  
Lorena Padilla ◽  
Cristina Pérez-Patiño ◽  
Juan M. Vazquez ◽  
Emilio A. Martínez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm ◽  
Plasma Cytokines

Proteins and Heparin Binding Proteins of Spermatozoa and Seminal Plasma in Beetal Bucks: Purification, Characterization and Role in In vivo Fertility

2020 ◽  
Author(s):  
Ranjna S. Cheema ◽  
Navjot S. Dhillon ◽  
Sumit Singhal
Keyword(s):  
Seminal Plasma ◽  
Binding Proteins ◽  
Proteome Analysis ◽  
Fertility Rate ◽  
Heparin Binding ◽  
Sds Page ◽  
Sperm Extract ◽  
Sperm Membrane ◽  
Special Relevance

Background: The proteome analysis of seminal plasma and spermatozoa is of special relevance in livestock. Heparin binding proteins (HBPs) found in the seminal plasma of several mammals are shown to bind to sperm membrane and affect a series of events that contribute to normal fertility, such as sperm capacitation, formation of the oviduct reservoir and binding to the oocyte. Profiles of HBPs from seminal plasma and sperm membranes have been associated with sperm fertility. Although, HBPs present in the SP are described in several species, but little is known about HBPs in buck. Methods: Seminal plasma (SP) and sperm extracts (SE) of 13 bucks were subjected to heparin-sepharose affinity chromatography. Sperm extract, seminal plasma and purified HBPs and Non-HBPs were fractionated by SDS-PAGE. Total 78 females (6 per buck) were mated with 13 bucks. Bucks were divided into two groups, G-I (high fertile, 83.3-100% FR) and G-II (low fertile, 50-66.7% FR). Relationship between HBPs and fertility rate was observed. Result: SDS – PAGE of SP and SE resulted in resolution of 22 (10-240 kDa) and 21 (10-270 kDa) bands, respectively. Based on fertility rate 15 and 13 kDa proteins were absent in SP of higher number of GI-compared to G-II bucks. Fourteen bands ranging from 10 – 180 kDa and 10 – 150 kDa were separated from SP-NHBP and SP-HBP. SP-HBPs of 75, 35, 30, 28, 25 and 13 kDa were present in higher (28.6%, 42.5%, 26.2%, 40.5%, 14.3% and 36.2%) number of high fertile than low fertile bucks. NHBP and HBP purified from SE resolved into 11 bands ranging from 10 – 135 kDa and 10 – 120 kDa, respectively. SE-HBP of 53 kDa, 50/45 kDa and 25 kDa were present in higher percentage of high fertile than low fertile bucks.

scholarly journals Sperm transport and retention at the fertilization site is orchestrated by a chemical guidance and oviduct movement

Reproduction ◽  
2012 ◽  
Vol 143 (5) ◽  
pp. 587-596 ◽  
Author(s):  
H A Guidobaldi ◽  
M E Teves ◽  
D R Uñates ◽  
L C Giojalas
Keyword(s):  
Evolutionary Adaptation ◽  
Sperm Transport ◽  
Working Model ◽  
Combined Action ◽  
Oviductal Fluid ◽  
Rabbit Spermatozoa

In mammals, only a few spermatozoa arrive at the fertilization site. During the last step in the journey to the egg, apart from their self-propulsion, spermatozoa may be assisted by oviduct movement and/or a guidance mechanism. The proportion of rabbit spermatozoa that arrive at the fertilization site was determined under in vivo conditions, in which either the ovulation products (secreting chemoattractants) and/or the oviduct movement (causing the displacement of the oviductal fluid) was inhibited. When only one of these components was inhibited, sperm transport to the fertilization site was partially reduced. However, when both the ovulation products and the oviduct movement were inhibited, almost no spermatozoa arrived at the fertilization site. The results suggest that spermatozoa are transported to and retained at the fertilization site by the combined action of a chemical guidance and the oviduct movement. A working model is proposed to explain how these two mechanisms may operate to transport spermatozoa to the fertilization site, probably as an evolutionary adaptation to maximize the chance of fertilizing an egg.

Effects of porcine pre-ovulatory oviductal fluid on boar sperm function

2010 ◽  
Vol 74 (4) ◽  
pp. 632-642 ◽  
Author(s):  
P. Coy ◽  
R. Lloyd ◽  
R. Romar ◽  
N. Satake ◽  
C. Matas ◽  
...  
Keyword(s):  
Sperm Function ◽  
Boar Sperm ◽  
Oviductal Fluid
Sign in / Sign up

Export Citation Format

Share Document