Cryopreservation of Fagus crenata seeds: estimation of optimum moisture content for maintenance of seed viability by Bayesian modeling

2018 ◽  
Vol 48 (2) ◽  
pp. 192-196 ◽  
Author(s):  
Keita Endoh ◽  
Michinari Matsushita ◽  
Megumi K. Kimura ◽  
So Hanaoka ◽  
Yuko Kurita ◽  
...  
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Fagus Crenata ◽  
Natural Forests ◽  
Fagus Crenata Blume ◽  
Long Term Storage ◽  
High Viability ◽  
Term Storage ◽  
Seed Cryopreservation

Fagus crenata Blume is an important forestry tree species that plays a key biological role in natural forests. Because F. crenata forests are declining under global warming, the conservation of seed resources is necessary to maintain sustainable regeneration. We examined the capability of seed cryopreservation to achieve semi-permanent preservation of the F. crenata genetic resource. Seeds at 7%–26% moisture content (MC) exhibited greater than 64% viability, with developing radicles growing at 22 °C in 16 h photoperiods following chilling at 2 °C for 2 months. These seeds were then stored cryogenically at –170 °C for 6 months; cryopreservation at 26% MC reduced seed viability to 12%, whereas dry seeds (7%–14% MC) maintained viability and were comparable with seeds that had neither been dried nor cryopreserved. Drying seeds to 6% MC reduced viability to 47%; following cryopreservation, the viability of these seeds with such a low MC was 36%. Using a Bayesian model, we then determined that the optimal range of MC for the cryopreservation of F. crenata seeds was 9%–12%. We conclude that cryopreservation is feasible for the long-term storage of F. crenata seeds, drying of the seeds to the range of optimal MC retains high viability during cryogenic storage.

scholarly journals Barley Seeds miRNome Stability during Long-Term Storage and Aging

2021 ◽  
Vol 22 (9) ◽  
pp. 4315
Author(s):  
Marta Puchta ◽  
Jolanta Groszyk ◽  
Magdalena Małecka ◽  
Marek D. Koter ◽  
Maciej Niedzielski ◽  
...  
Keyword(s):  
Seed Viability ◽  
Small Rna Sequencing ◽  
The Novel ◽  
Seed Aging ◽  
Long Term Storage ◽  
Novel Mirna ◽  
Complex Biological Process ◽  
Almost All ◽  
Term Storage

Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state.

scholarly journals The Technological Investigations Of Manufacturing Of Energy Chips

2015 ◽  
Vol 1 ◽  
pp. 220
Author(s):  
Ziedonis Miklašēvičs
Keyword(s):  
Moisture Content ◽  
Dry Mass ◽  
Quality Parameters ◽  
Heating Value ◽  
Long Term Storage ◽  
Lower Heating Value ◽  
Term Storage ◽  
Lower Heating

The methodology in Latvia forest industry provide to determine the quality of energy chips only in long- term storage places before selling. Due to the lack of hard empirical data about the quality parameters of energy chips in different phases of manufacturing process, this research paper consists of: - the identification and analyses of the factors that influenced the values of energy chips quality features such as: bulk density, moisture content, ash content, higher and lower heating value according to actual moisture content and per dry mass of the chips; - the methodology for determination the quality parameters of energy chips by analysis the wood moisture content and by choice the method of the manufacturing of energy chips.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

Critical review of threatened species collections in the New South Wales Seedbank: implications for ex situ conservation of biodiversity

2004 ◽  
Vol 10 (4) ◽  
pp. 221 ◽  
Author(s):  
C. A. Offord ◽  
M. L. McKensy ◽  
P. V. Cuneo
Keyword(s):  
Moisture Content ◽  
Threatened Species ◽  
New South ◽  
Ex Situ ◽  
Seed Moisture Content ◽  
Long Term Storage ◽  
South Wales ◽  
Seed Moisture ◽  
Term Storage

This article reviews the germinability and viability of seeds of threatened species collections in the New South Wales (NSW) Seedbank with the manifold aims of: ensuring that existing storage treatments and conditions provide effective ex situ storage of threatened species seed; providing baseline viability and seed storage life data on threatened species; and, identifying research gaps in seed germination and storage protocols for threatened species and communities. The germinability and viability of a range of seed accessions, of various ages and stored under different (although mainly identifiable) conditions in the NSW Seedbank, was determined through germination and cut-tests. The results indicated that many of the Fabaceae, Myrtaceae and Proteaceae species tested are orthodox and can be stored at 5 to 10% moisture content at 5�C for up to ten years without significant loss of viability (short- to medium-term storage). The best results were obtained in the lower seed moisture content range (2 to 9%), which appeared to be especially critical for long-term storage of many Proteaceae accessions, reinforcing the need to attain the correct seed moisture content for long-term storage. Around 10% of accessions exhibited some degree of dormancy even after long storage periods. Storage of the widest range of species, for periods greater than ten years for long-term conservation purposes, is generally best conducted by storing at sub-zero temperatures. Freezing at -18�C had little effect on the germinability of a range of seeds tested and is recommended over storage at 5�C. Collection and seed banking procedures for the NSW Seedbank will be regularly reviewed and procedures modified in order to identify the best long-term storage conditions for species within this and other seedbanks. Seed collection strategies to maximize diversity and uses of seedbanks in conservation are discussed.

scholarly journals Localization and Dynamics of the Methionine Sulfoxide Reductases MsrB1 and MsrB2 in Beech Seeds

2021 ◽  
Vol 22 (1) ◽  
pp. 402
Author(s):  
Natalia Wojciechowska ◽  
Agnieszka Bagniewska-Zadworna ◽  
Julia Minicka ◽  
Kornel M. Michalak ◽  
Ewa M. Kalemba
Keyword(s):  
Stress Responses ◽  
Management Practices ◽  
Seed Viability ◽  
Methionine Sulfoxide ◽  
Embryonic Axes ◽  
Long Term Storage ◽  
Protein Storage Vacuoles ◽  
Methionine Sulfoxide Reductases ◽  
Term Storage

Beech seeds are produced irregularly, and there is a need for long-term storage of these seeds for forest management practices. Accumulated reactive oxygen species broadly oxidize molecules, including amino acids, such as methionine, thereby contributing to decreased seed viability. Methionine oxidation can be reversed by the activity of methionine sulfoxide reductases (Msrs), which are enzymes involved in the regulation of many developmental processes and stress responses. Two types of Msrs, MsrB1 and MsrB2, were investigated in beech seeds to determine their abundance and localization. MsrB1 and MsrB2 were detected in the cortical cells and the outer area of the vascular cylinder of the embryonic axes as well as in the epidermis and parenchyma cells of cotyledons. The abundances of MsrB1 and MsrB2 decreased during long-term storage. Ultrastructural analyses have demonstrated the accumulation of these proteins in protein storage vacuoles and in the cytoplasm, especially in close proximity to the cell membrane. In silico predictions of possible Msr interactions supported our findings. In this study, we investigate the contribution of MsrB1 and MsrB2 locations in the regulation of seed viability and suggest that MsrB2 is linked with the longevity of beech seeds via association with proper utilization of storage material.

scholarly journals SEED QUALITY EVALUATION IN THE COLLECTION OF CYAMOPSIS TETRAGONOLOBA AFTER LONG¬TERM STORAGE UNDER UNCONTROLLED CONDITIONS

2020 ◽  
Vol 180 (4) ◽  
pp. 48-58
Author(s):  
N. V. Rakovskaya ◽  
O. N. Zabegayeva ◽  
E. A. Dzyubenko
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Growing Season ◽  
Cyamopsis Tetragonoloba ◽  
Monthly Temperature ◽  
Germination Energy ◽  
Long Term Storage ◽  
Seed Hardness ◽  
Term Storage

Background. Guar or clusterbean (Cyamopsis tetragonoloba (L.) Taub.) is an annual leguminous plant cultivated for feed, food and industrial purposes. Its seed endosperm is used to extract guar gum, so the guar genetic resources stored in the VIR collection are of strategic importance for the import substitution policy in Russia in the context of their prospective introduction into cultivation in the country’s southern areas. Most of the guar accessions had been preserved by VIR for 40 years or more, so the task was to assess their germination rates and restore their viability. In the process of screening, seed quality parameters of the accessions were examined and analyzed.Materials and methods. VIR’s collection of C. tetragonoloba, initiated by N. I. Vavilov, now contains 111 accessions. Percentage seed viability, germination energy and seed hardness were evaluated in the Long-Term Storage Laboratory for 50 accessions reproduced in 2018 and for 263 older reproductions of 89 guar accessions. In total, 313 seed samples reproduced across 18 different years were analyzed. Statistica 10 software was used to perform a single-factor analysis of variance and find out how germination energy, seed viability and seed hardness correlated with the age of seeds, mean monthly temperature and mean rainfall during the growing season.Results and discussion. After more than 40 years of storage, the guar accessions did not entirely lost their viability under laboratory and field conditions. Very high positive correlations were found for their percentage viability and germination energy with the mean monthly temperature in the year of reproduction. A weak positive correlation was observed between seed hardness and the year of reproduction. Correlations between seed viability and rainfall during the growing season were weak, with a weak negative correlation between seed hardness and rainfall. The viability level of 30-year-old seeds was 50%, so guar may be regarded as belonging to the group of macrobiotic plants.

scholarly journals Does Storage under Gene Bank Conditions Affect Seed Germination and Seedling Growth? The Case of Senecio morisii (Asteraceae), a Vascular Plant Exclusive to Sardinian Water Meadows

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 581 ◽  
Author(s):  
Alba Cuena-Lombraña ◽  
Martina Sanna ◽  
Marco Porceddu ◽  
Gianluigi Bacchetta
Keyword(s):  
Seedling Growth ◽  
Seedling Survival ◽  
Vascular Plant ◽  
Seed Viability ◽  
Storage Conditions ◽  
Growth And Survival ◽  
Survival Percentage ◽  
Long Term Storage ◽  
Term Storage

Understanding seed viability under long-term storage conditions provides basic and useful information to investigate the effectiveness of seed banking. Besides the germination success, seedling establishment is also an important requirement, and a decisive step to ensure plant propagation. We used comparative data of germination, seedling growth, and survival percentage between fresh and 10-years-stored seeds of Senecio morisii, a narrow endemic and vulnerable species of Sardinia (Italy), in order to evaluate if differences exist in these traits between fresh and 10-years-stored seeds. Stored seeds showed higher germination percentages than fresh ones, whereas seedling growth and survival did not present significant differences between them, except for seedling growth in plants produced from seeds germinated at 25 °C. This study allowed us to assess if seeds of S. morisii were able to germinate under controlled conditions, and if they maintained their viability and germination capacity for at least 10 years of long-term storage in the seed bank. In addition, the high seedling survival detected in both fresh and stored seeds suggests that stored seeds of S. morisii can be used to support reinforcement or reintroduction actions when fresh materials are not available.

scholarly journals The Influence of Moisture Content and Temperature on the Long-Term Storage Stability of Freeze-Dried High Concentration Immunoglobulin G (IgG)

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 303 ◽  
Author(s):  
Arnold Duralliu ◽  
Paul Matejtschuk ◽  
Paul Stickings ◽  
Laura Hassall ◽  
Robert Tierney ◽  
...  
Keyword(s):  
Moisture Content ◽  
Immunoglobulin G ◽  
Storage Stability ◽  
Binding Activity ◽  
Atmospheric Environment ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Monomer Content ◽  
Term Storage

High protein concentration products for targeted therapeutic use are often freeze-dried to enhance stability. The long-term storage stability of freeze-dried (FD) plasma-derived Immunoglobulin G (IgG) from moderate to high concentrations (10–200 mg/mL) was assessed. Monomer content, binding activity and reconstitution times were evaluated over a 12-month period under accelerated and real-term storage conditions. In the first case study it was shown that FD IgG from 10 to 200 mg/mL had minimal monomer/activity losses at up to ambient temperature after 12 months of storage. However, at 45 °C the sucrose-to-protein ratio played a significant impact on IgG stability above 50 mg/mL. All IgG concentrations witnessed moisture ingress over a 12-month period. The impact of moisture ingress from environmental exposure (between 0.1% and 5% w/w moisture) for IgG 50 mg/mL was assessed, being generated by exposing low moisture batches to an atmospheric environment for fixed time periods. Results showed that at −20 °C and 20 °C there was no significant difference in terms of monomer or antigen-binding activity losses over 6 months. However, at 45 °C, there were losses in monomer content, seemingly worse for higher moisture content samples although model binding activity indicated no losses. Finally, the difference between a low moisture product (0.1–1% w/w) and a moderately high moisture (3% w/w) product generated by alternative freeze-drying cycles, both stoppered under low oxygen headspace conditions, was evaluated. Results showed that at −20 °C and 20 °C there was no difference in terms of binding activity or monomer content. However, at 45 °C, the low moisture samples had greater monomer and binding activity losses than samples from the highest moisture cycle batch, indicating that over-drying can be an issue.

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