scholarly journals Effect of Selected Anionic and Cationic Drugs Affecting the Central Nervous System on Electrical Properties of Phosphatidylcholine Liposomes: Experiment and Theory

2021 ◽  
Vol 22 (5) ◽  
pp. 2270
Author(s):  
Joanna Kotyńska ◽  
Monika Naumowicz
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Barbituric Acid ◽  
Membrane Surface ◽  
Adsorption Equilibria ◽  
Two Component ◽  
The Central Nervous System

Interactions between phospholipid membranes and selected drugs affecting the central nervous system (CNS) were investigated. Small, unilamellar liposomes were used as biomimetic cell membrane models. Microelectrophoretic experiments on two-component liposomes were performed using the electrophoretic light scattering technique (ELS). The effect of both positively (perphenazine, PF) and negatively (barbituric acid, BA) charged drugs on zwitterionic L-α-phosphatidylcholine (PC) membranes were analyzed. Experimental membrane surface charge density (d) data were determined as a function of pH. Quantitative descriptions of the adsorption equilibria formed due to the binding of solution ions to analyzed two-component membranes are presented. Binding constants of the solution ions with perphenazine and barbituric acid-modified membranes were determined. The results of our research show that both charged drugs change surface charge density values of phosphatidylcholine membranes. It can be concluded that perphenazine and barbituric acid are located near the membrane surface, interacting electrostatically with phosphatidylcholine polar heads.

scholarly journals Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

2020 ◽  
Vol 21 (18) ◽  
pp. 6972 ◽  
Author(s):  
Monika Naumowicz ◽  
Magdalena Kusaczuk ◽  
Marcin Zając ◽  
Miroslav Gál ◽  
Joanna Kotyńska
Keyword(s):  
Charge Density ◽  
Surface Charge ◽  
Phenolic Acids ◽  
Surface Charge Density ◽  
Membrane Surface ◽  
Theoretical Data ◽  
Beneficial Effects ◽  
Ph Values ◽  
Ic50 Values ◽  
Human Gbm

Cinnamic acid (CA) and ferulic acid (FA) are naturally occurring phenolic acids claimed to exert beneficial effects against disorders related to oxidative stress, including cancer. One such malignancy that still remains a therapeutic challenge mainly due to its heterogeneity and inaccessibility to therapeutic agents is Glioblastoma multiforme (GBM). Here, the influence of CA and FA on the surface charge density of human GBM cell line LN-229 was studied using the electrophoretic light scattering technique. Also, the cytotoxicity of both phenolic acids was determined by metabolic activity-assessing tetrazolium test (MTT) analysis after exposure to CA and FA for 24 h and 48 h. Results showed that both compounds reduced cell viability of LN-229 cells, with more pronounced effect evoked by CA as reflected in IC50 values. Further analyses demonstrated that, after treatment with both phenolic acids, the negative charge of membranes decreased at high pH values and the positive charge of the membranes increased at low pH values compared to the data obtained for untreated cells. Afterward, a four-equilibrium model was applied to estimate the total surface concentrations of both acidic and basic functional groups and their association constants with solution ions in order to calculate theoretical values of membrane surface charge densities. Then, the theoretical data were compared to the experimental data in order to verify the mathematical model. As such, our results indicate that application of electrochemical methods to determine specific drug–membrane interactions might be crucial for predicting their pharmacological activity and bioavailability.

scholarly journals The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

2019 ◽  
Vol 20 (21) ◽  
pp. 5286 ◽  
Author(s):  
Marcin Andrzej Kruszewski ◽  
Joanna Kotyńska ◽  
Magdalena Kusaczuk ◽  
Miroslav Gál ◽  
Monika Naumowicz
Keyword(s):  
Charge Density ◽  
Surface Charge ◽  
Cancer Cells ◽  
Functional Groups ◽  
Surface Charge Density ◽  
Cell Membranes ◽  
Membrane Surface ◽  
Glioblastoma Cell ◽  
Coumaric Acid ◽  
Human Glioblastoma Cell

p-Coumaric acid (p-CoA), a phenolic acid belonging to the hydroxycinnamic acids family, is a compound with tentative anticancer potential. Microelectrophoretic mobility measurements conducted at various pH values of electrolyte solution were applied to study p-CoA effects on electrical properties of human glioblastoma cell membranes. The obtained results demonstrated that after the p-CoA treatment, the surface charge density of cancer cells changed in alkaline pH solutions, while no noticeable changes were observed in cell membranes incubated with p-CoA compared to control at acidic pH solutions. A four-equilibrium model was used to describe the phenomena occurring on the cell membrane surface. The total surface concentrations of both acidic and basic functional groups and their association constants with solution ions were calculated and used to define theoretical curves of membrane surface charge density versus pH. The resulting theoretical curves and the experimental data were compared to verify the reliability and validity of the adopted model. The deviation of both kinds of data obtained at a higher pH may be caused by disregarding interactions between the functional groups of cancer cells. Processes occurring in the cell membranes after their incubation with p-CoA can lead to disorders of existing equilibria, which result in changes in values of the parameters describing these equilibria.

scholarly journals Cadmium-Induced Oxidative Stress: Focus on the Central Nervous System

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 492 ◽  
Author(s):  
Jacopo J. V. Branca ◽  
Claudia Fiorillo ◽  
Donatello Carrino ◽  
Ferdinando Paternostro ◽  
Niccolò Taddei ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Membrane Surface ◽  
Cell Types ◽  
Environmental Pollutant ◽  
Glutathione Depletion ◽  
Ros Generation ◽  
The Central Nervous System ◽  
Cd Exposure ◽  
Cell Membrane Surface

Cadmium (Cd), a category I human carcinogen, is a well-known widespread environmental pollutant. Chronic Cd exposure affects different organs and tissues, such as the central nervous system (CNS), and its deleterious effects can be linked to indirect reactive oxygen species (ROS) generation. Since Cd is predominantly present in +2 oxidation state, it can interplay with a plethora of channels and transporters in the cell membrane surface in order to enter the cells. Mitochondrial dysfunction, ROS production, glutathione depletion and lipid peroxidation are reviewed in order to better characterize the Cd-elicited molecular pathways. Furthermore, Cd effects on different CNS cell types have been highlighted to better elucidate its role in neurodegenerative disorders. Indeed, Cd can increase blood–brain barrier (BBB) permeability and promotes Cd entry that, in turn, stimulates pericytes in maintaining the BBB open. Once inside the CNS, Cd acts on glial cells (astrocytes, microglia, oligodendrocytes) triggering a pro-inflammatory cascade that accounts for the Cd deleterious effects and neurons inducing the destruction of synaptic branches.

scholarly journals Theoretical Considerations and the Microelectrophoresis Experiment on the Influence of Selected Chaotropic Anions on Phosphatidylcholine Membrane Surface Charge Density

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 132
Author(s):  
Joanna Kotyńska ◽  
Monika Naumowicz
Keyword(s):  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Membrane Surface ◽  
Quantitative Description ◽  
Theoretical Data ◽  
Lipid Vesicles ◽  
Hofmeister Series ◽  
Chaotropic Anions ◽  
Membrane Surface Charge

Influence of sodium salts of selected chaotropic anions from the Hofmeister series (NaCl, NaBr, NaNO3, NaI) on the surface charge density of phosphatidylcholine membranes was studied. Small unilamellar lipid vesicles were used as a model system in the investigations. The theoretical and experimental approach to the interactions between inorganic anions and phosphatidylcholine membranes is presented. Experimental membrane surface charge densities data were determined as a function of pH of the aqueous electrolytes using microelectrophoresis method. The quantitative description of the interactions between zwitterionic phosphatidylcholine membrane and monovalent anions is presented. The equilibria constants of the binding of solution ions onto phospholipid surface were calculated. Knowledge of these parameters was essential to determine the theoretical membrane surface charge density values. The theoretical data were compared to the experimental ones in order to verify the mathematical model. Both approaches indicate that the anion-phosphatidylcholine membrane interaction increases with the size of the anion. The adsorption of chaotropic anions to membranes was found to follow the Hofmeister series I− > NO3− > Br− > Cl−.

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