scholarly journals Application of microRNA in Human Osteoporosis and Fragility Fracture: A Systemic Review of Literatures

2021 ◽  
Vol 22 (10) ◽  
pp. 5232
Author(s):  
Yen-Zung Wu ◽  
Hsuan-Ti Huang ◽  
Tsung-Lin Cheng ◽  
Yen-Mou Lu ◽  
Sung-Yen Lin ◽  
...  
Keyword(s):  
Human Blood ◽  
Diagnostic Value ◽  
Systemic Review ◽  
Blood Samples ◽  
In Vivo Experiments ◽  
Pubmed Database ◽  
Entry Points ◽  
Human Blood Samples

MicroRNAs (miRNAs) could serve as ideal entry points to the deregulated pathways in osteoporosis due to their relatively simple upstream and downstream relationships with other molecules in the signaling cascades. Our study aimed to give a comprehensive review of the already identified miRNAs in osteoporosis from human blood samples and provide useful information for their clinical application. A systematic literature search for relevant studies was conducted in the Pubmed database from inception to December 2020. We set two essential inclusion criteria: human blood sampling and design of controlled studies. We sorted the results of analysis on human blood samples according to the study settings and compiled the most promising miRNAs with analyzed diagnostic values. Furthermore, in vitro and in vivo evidence for the mechanisms of the identified miRNAs was also illustrated. Based on both diagnostic value and evidence of mechanism from in vitro and in vivo experiments, miR-23b-3p, miR-140-3p, miR-300, miR-155-5p, miR-208a-3p, and miR-637 were preferred candidates in diagnostic panels and as therapeutic agents. Further studies are needed to build sound foundations for the clinical usage of miRNAs in osteoporosis.

scholarly journals Protein Adductomics: Methodologies for Untargeted Screening of Adducts to Serum Albumin and Hemoglobin in Human Blood Samples

2019 ◽  
Vol 8 (1) ◽  
pp. 6 ◽  
Author(s):  
Henrik Carlsson ◽  
Stephen Rappaport ◽  
Margareta Törnqvist
Keyword(s):  
Mass Spectrometry ◽  
Serum Albumin ◽  
Human Blood ◽  
Mass Spectrometry Data ◽  
Reaction Products ◽  
Blood Samples ◽  
Large Numbers ◽  
Human Blood Samples ◽  
Critical Aspects

The reaction products of electrophiles in vivo can be measured as adducts to the abundant proteins, hemoglobin (Hb), and human serum albumin (HSA), in human blood samples. During the last decade, methods for untargeted screening of such adducts, called “adductomics”, have used liquid chromatography-mass spectrometry to detect large numbers of previously unknown Hb and HSA adducts. This review presents methodologies that were developed and used in our laboratories for Hb and HSA adductomics, respectively. We discuss critical aspects regarding choice of target protein, sample preparation, mass spectrometry, data evaluation, and strategies for identification of detected unknown adducts. With this review we give an overview of these two methodologies used for protein adductomics and the precursor electrophiles that have been elucidated from the adducts.

scholarly journals Platelet-neutrophil complex formation-a detailed in vitro analysis of murine and human blood samples

2015 ◽  
Vol 99 (5) ◽  
pp. 781-789 ◽  
Author(s):  
Maximilian Mauler ◽  
Julia Seyfert ◽  
David Haenel ◽  
Hannah Seeba ◽  
Janine Guenther ◽  
...  
Keyword(s):  
Complex Formation ◽  
Human Blood ◽  
Blood Samples ◽  
Human Blood Samples ◽  
Vitro Analysis ◽  
In Vitro Analysis

Stability of Fibrinogen Concentrate in Human Blood Samples: An In Vitro Study

2018 ◽  
Vol 183 (suppl_1) ◽  
pp. 183-188 ◽  
Author(s):  
Wenjun Z Martini ◽  
Rodolfo de Guzman ◽  
Michael A Dubick
Keyword(s):  
Human Blood ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fibrinogen Concentrate ◽  
Blood Samples ◽  
Human Blood Samples

scholarly journals The Role of Thermal Effects in Plasma Medical Applications: Biological and Calorimetric Analysis

2019 ◽  
Vol 9 (24) ◽  
pp. 5560 ◽  
Author(s):  
Luigi Cordaro ◽  
Gianluca De Masi ◽  
Alessandro Fassina ◽  
Clarice Gareri ◽  
Antonio Pimazzoni ◽  
...  
Keyword(s):  
Thermal Effects ◽  
Plasma Source ◽  
Total Power ◽  
Therapeutic Effects ◽  
Calorimetric Analysis ◽  
Blood Samples ◽  
In Vivo Experiments ◽  
Male Wistar Rats

Plasma Medicine tools exploit the therapeutic effects of the exposure of living matter to plasma produced at atmospheric pressure. Since these plasmas are usually characterized by a non-thermal equilibrium (highly energetic electrons, low temperature ions), thermal effects on the substrate are usually considered negligible. Conversely, reactive oxygen and nitrogen species (RONS), UV radiation and metastables are thought to play a major role. In this contribution, we compare the presence of thermal effects in different operational regimes (corresponding to different power levels) of the Plasma Coagulation Controller (PCC), a plasma source specifically designed for accelerating blood coagulation. In particular, we analyze the application of PCC on human blood samples (in vitro) and male Wistar rats tissues (in vivo). Histological analysis points out, for the highest applied power regime, the onset of detrimental thermal effects such as red cell lysis in blood samples and tissues damages in in-vivo experiments. Calorimetric bench tests performed on metallic targets show that the current coupled by the plasma on the substrate induces most of measured thermal loads through a resistive coupling. Furthermore, the distance between the PCC nozzle and the target is found to strongly affect the total power.

A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

2020 ◽  
Vol 8 (6) ◽  
pp. 1310-1315 ◽  
Author(s):  
Manlin Fu ◽  
Wei Shen ◽  
Yajun Chen ◽  
Wenjun Yi ◽  
Chunhui Cai ◽  
...  
Keyword(s):  
Human Blood ◽  
Fluorescent Probes ◽  
Living Cells ◽  
The State ◽  
Blood Samples ◽  
Highly Sensitive ◽  
Human Blood Samples ◽  
Viscosity Changes

Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo.

scholarly journals Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe3+ in human blood samples and cells

RSC Advances ◽  
2019 ◽  
Vol 9 (47) ◽  
pp. 27439-27448 ◽  
Author(s):  
Vishaka V. H. ◽  
Manav Saxena ◽  
Geetha Balakrishna R. ◽  
Sachin Latiyan ◽  
Shilpee Jain
Keyword(s):  
Fluorescent Probe ◽  
Human Blood ◽  
Blood Samples ◽  
Turn On ◽  
Human Blood Samples

The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro.

Sign in / Sign up

Export Citation Format

Share Document