scholarly journals The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin

2021 ◽  
Vol 22 (16) ◽  
pp. 8931
Author(s):  
Diane M. Spencer ◽  
Angel Garza Reyna ◽  
David S. Pisetsky
Keyword(s):  
Micrococcus Luteus ◽  
Double Helix ◽  
Lambda Phage ◽  
E Coli ◽  
Base Modification ◽  
Species Origin ◽  
Left Handed ◽  
Calf Thymus ◽  
Dna Antibodies ◽  
Z Dna

DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (Micrococcus luteus, calf thymus, Escherichiacoli, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and E. coli DNA showed binding with the monoclonal anti-DNA preparation. The specificity for Z-DNA conformation in MC DNA was demonstrated by an inhibition binding assay. An algorithm to identify propensity to form Z-DNA indicated that DNA from Mycobacterium tuberculosis could form Z-DNA, a prediction confirmed by immunoassay. Together, these findings indicate that anti-Z-DNA antibodies can serve as probes for the presence of Z-DNA in DNA of various species origin and that the content of Z-DNA varies significantly among DNA sources.

scholarly journals The molecular structure of the left-handed Z-DNA double helix at 1.0-Å atomic resolution

1989 ◽  
Vol 264 (14) ◽  
pp. 7921-7935
Author(s):  
R V Gessner ◽  
C A Frederick ◽  
G J Quigley ◽  
A Rich ◽  
A H J Wang
Keyword(s):  
Molecular Structure ◽  
Double Helix ◽  
Atomic Resolution ◽  
Left Handed ◽  
Z Dna ◽  
Dna Double Helix

Filarial DNA and its interaction with polyamines and antifilarial drugs

1997 ◽  
Vol 71 (4) ◽  
pp. 325-332 ◽  
Author(s):  
U. Pandya ◽  
J.K. Saxena ◽  
O.P. Shukla
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Melting Temperature ◽  
Base Composition ◽  
Micrococcus Luteus ◽  
Gc Content ◽  
Setaria Cervi ◽  
Calf Thymus ◽  
Absorption Studies ◽  
Z Dna

AbstractThe interaction of DNA from filarial parasite Setaria cervi with polyamines was monitored by melting temperature (Tm) profile, condensation and B to Z DNA transition and compared with DNA of Escherichia coli, Micrococcus luteus and calf thymus having different GC content. Polyamines, viz. spermine and spermidine, stabilized the secondary structure of all DNAs as indicated by increase in Tm value. UV absorption studies indicated B to Z DNA transition in the presence of polyamines. The amount of polyamines required for B to Z transition was dependent upon base composition of DNA and charge of the polyamine. Filarial DNA (AT rich) required six times higher concentration of spermine as compared to GC rich DNA for B to Z DNA transition. Spermidine was not effective in causing transition of S. cervi DNA even at Spd:DNA-P ratio of 20. The antifilarial compound suramin significantly decreased melting temperature of filarial DNA as compared to GC rich DNAs of other parasites. Suramin adversely affected condensation and B to Z DNA transition of various DNAs but prior addition of polyamines protected the DNAs from the destabilizing effect of suramin.

High-salt d(CpGpCpG), a left-handed Z′ DNA double helix

Nature ◽  
1980 ◽  
Vol 286 (5773) ◽  
pp. 567-573 ◽  
Author(s):  
Horace Drew ◽  
Tsunehiro Takano ◽  
Shoji Tanaka ◽  
Keiichi Itakura ◽  
Richard E. Dickerson
Keyword(s):  
Double Helix ◽  
High Salt ◽  
Left Handed ◽  
Z Dna ◽  
Dna Double Helix

scholarly journals Mendelian Disease caused by variants affecting recognition of Z-DNA and Z-RNA by the Zα domain of the double-stranded RNA Editing Enzyme ADAR

2019 ◽  
Author(s):  
Alan Herbert
Keyword(s):  
Double Helix ◽  
Protein Isoforms ◽  
Mendelian Disease ◽  
Loss Of Function ◽  
Double Stranded Rna ◽  
Parental Chromosome ◽  
Dna And Rna ◽  
Left Handed ◽  
Z Dna ◽  
Editing Enzyme

Variants in the human double-stranded RNA (dsRNA) editing enzyme ADAR produce three well-characterized rare Mendelian Diseases: Dyschromatosis Symmetrica Hereditaria (DSH)(OMIM: 127400), Aicardi-Goutières syndrome (AGS)(OMIM: 615010) and Bilateral Striatal Necrosis/Dystonia (BSD). ADAR encodes p150 and p110 protein isoforms. p150 incorporates the Zα domain that binds left-handed Z-DNA and Z-RNA with high affinity through contact of highly conserved residues with the DNA and RNA double-helix. In certain individuals, frameshift variants on one parental chromosome in the second exon of ADAR produce haploinsufficiency of p150 while maintaining normal expression of p110. In other individuals, loss of p150 expression from one chromosome allows mapping of Zα p150 variants from the other parental chromosome directly to phenotype. The analysis reveals that loss of function Zα variants cause dysregulation of innate interferon responses to dsRNA. This approach confirms a biological role for the left-handed conformation in human disease, further validating the power of Mendelian genetics to provide unambiguous answers. The findings reveal that the human genome encodes genetic information using both shape and sequence.

scholarly journals ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS

1965 ◽  
Vol 121 (2) ◽  
pp. 309-321 ◽  
Author(s):  
Charles L. Christian ◽  
Arthur R. DeSimone ◽  
John L. Abruzzo
Keyword(s):  
Low Molecular Weight ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Sperm Dna ◽  
Calf Thymus ◽  
Dnase Treatment ◽  
Dna Antibodies ◽  
Rabbit Bone ◽  
Salmon Sperm Dna

Complement-fixing anti-DNA antibodies were detected in a minority of sera of rabbits hyperimmunized with killed Gram-negative bacteria. The C'-fixing property of DNA was lost after DNase treatment. Preferential reactivity with denatured DNA was observed. The antisera reacted with DNA preparations derived from rabbit bone marrow and thymus, calf thymus, pneumococci, salmon sperm, and Escherichia coli. E. coli DNA was less effective than preparations of mammalian and salmon sperm DNA in fixation of C'. Inhibition of DNA C' fixation by nucleotides and nucleosides was observed. The bulk of anti-DNA activity was associated with the low molecular weight antibody fraction.

EFECTO INHIBITORIO DE EXTRACTOS DE Vitex mollis Kunth CONTRA BACTERIAS Y ESPECIES DE Fusarium DE IMPORTANCIA HUMANA Y AGRÍCOLA

2018 ◽  
Vol 41 (4) ◽  
pp. 353-363
Author(s):  
Alberto J. Valencia-Botin ◽  
Melesio Gutiérrez-Lomelí ◽  
Juan A. Morales-Del-Río ◽  
Pedro J. Guerrero-Medina ◽  
Miguel A. Robles-García ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Enterica ◽  
Micrococcus Luteus ◽  
E Coli

Actualmente existe la necesidad de hacer frente al problema de la resistencia a los antibióticos y al uso indiscriminado de fungicidas químicos en la agricultura. El objetivo de este trabajo fue evaluar el efecto inhibitorio de extractos acuosos, metanólicos, acetónicos y hexánicos de hoja y tallo de Vitex mollis Kunth (Lamiaceae) contra diferentes bacterias (Escherichia coli, Micrococcus luteus, Salmonella enterica y Staphylococcus aureus) y especies del hongo Fusarium (F. verticillioides, F. oxysporum, F. tapsinum y F. oxysporum f.sp. lycopersici) de importancia en la salud y en la agricultura, así como determinar su composición química general. Se determinaron las concentraciones inhibitorias mínimas (CIM) de todos los extractos por la técnica de microdilución, excepto del hexánico, que no presentó inhibición en las bacterias estudiadas. S. enterica fue la bacteria que mostró mayor sensibilidad al extracto metanólico de tallo (CIM = 28 μg mL-1), le siguieron M. luteus (CIM = 32 μg mL-1), S. aureus (CIM = 75 μg mL-1) y E. coli (CIM = 80 μg mL- 1). Los extractos metanólicos y acuosos de tallo presentaron mayor porcentaje de inhibición contra los diferentes tipos de Fusarium evaluados por el método de dilución en agar. Los extractos de V. mollis inhibieron a F. verticillioides entre 62 y 91 % con 120 μg mL-1 de extracto. El orden de las especies de hongos inhibidas por los extractos fue: F. verticillioides > F. oxysporum > F. tapsinum > F. oxysporum f.sp. lycopersici. La composición química de las especies se determinó mediante pruebas para fenoles, taninos, flavonoides, triterpenos, alcaloides, cumarinas y saponinas. Ninguno de los extractos presentó alcaloides y saponinas. Los fenoles (37.1 mg EAG/g muestra seca) y flavonoides (26.8 mg EQ/g muestra seca) fueron los compuestos mayoritarios en los extractos metanólicos y acuosos. En conclusión, se requieren cantidades muy pequeñas de extracto para la inhibición de bacterias y de Fusarium; por lo tanto, V. mollis puede ser considerada una fuente de metabolitos para este fin y en la agricultura como control alternativo dentro de un manejo integrado de enfermedades.

scholarly journals A Left-Handed RNA Double Helix Bound by the Zα Domain of the RNA-Editing Enzyme ADAR1

Structure ◽  
2007 ◽  
Vol 15 (4) ◽  
pp. 395-404 ◽  
Author(s):  
Diana Placido ◽  
Bernard A. Brown ◽  
Ky Lowenhaupt ◽  
Alexander Rich ◽  
Alekos Athanasiadis
Keyword(s):  
Rna Editing ◽  
Double Helix ◽  
Left Handed ◽  
Editing Enzyme

The halting arrival of left-handed Z-DNA

2003 ◽  
Vol 60 (3) ◽  
pp. 418-423 ◽  
Author(s):  
C.E Gagna ◽  
W.C Lambert
Keyword(s):  
Left Handed ◽  
Z Dna
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