Phosphonates as Unique Components of Plant Seeds—A Promising Approach to Use Phosphorus Profiles in Plant Chemotaxonomy

Phosphorus is one of the most important elements essential for all living beings. Plants accumulate and store phosphorous in various forms that have diverse physiological and biochemical functions. In this study,we determine and then examine the phosphorus profiles of seeds of plants belonging to different taxa based on extractable inorganic phosphates and organic forms of phosphorus. We paid particular attention to the presence of natural phosphonates in the tested materials. The inorganic phosphates were determined colorimetrically, whereas phosphorus profiles were created by using 31P NMR spectroscopy. Our study on phosphorus profiles revealed that the obtainedsets of data vary significantly among the representatives of different taxa and were somehow specific for families of plants. It should be emphasised that the measurements obtained using 31P NMR spectroscopy undoubtedly confirmed—for the first time—the presence of phosphonates among the natural components of plant seeds. Hence, the classification of plants considering the phosphorus profiles, including the presence of phosphonates, may be a new additional chemotaxonomic feature.

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Использование ЯМР и ИК спектроскопии для исследования крови в целях диагностики стадии заболевания при диффузной патологии печени

Журнал технической физики ◽

10.21883/os.2020.06.49411.16-20 ◽

2020 ◽

Vol 128 (6) ◽

pp. 783

Author(s):

М.В. Кручинина ◽

В.Н. Кручинин ◽

Г.В. Шувалов ◽

И.В. Минин ◽

О.В. Минин

Keyword(s):

Nmr Spectroscopy ◽

Liver Fibrosis ◽

Blood Serum ◽

31P Nmr ◽

Alpha Helix ◽

Adenosine Monophosphate ◽

31P Nmr Spectroscopy ◽

Combined Use ◽

Inorganic Phosphates ◽

Severe Fibrosis

67 patients (mean age 48 ± 1 year) with diffuse liver diseases of various origins were examined. It has been established that an increase in the degree of fibrosis is associated with an increase in the resonance intensity of glucose-6-phosphates, adenosine monophosphate (AMP), inosine monophosphate (IMP), pyrophosphates, 2,3-DPH and a decrease in the resonances of inorganic phosphates and phosphocreatine, γ-, alpha- and beta- ATP, -UTP, Hb- and Mg-ATP, as well as beta- and alpha-ADP. The predictive value of a positive result of erythrocyte 31P NMR spectroscopy for liver fibrosis is 92.3% (sensitivity 85.71%, specificity 80%). In the IR spectra of the blood serum of patients with severe fibrosis, in contrast to moderate fibrosis, the resonance intensity is higher at 1280, 3190 and significantly lower at 776, 818, 889, 966, 1399.1450, 1570, 1635 cm-1, which indicates the presence of protein molecules with a secondary structure mainly in the form of a helix (alpha-helix) compared with the structure in the form of a folded sheet (beta-sheet) (p <0.001-0.05). The combined use of 31P NMR spectroscopy of erythrocyte suspensions and IR spectroscopy of blood serum made it possible to increase diagnostic accuracy (96%), sensitivity (97%) and specificity (91%) in distinguishing between degrees of liver fibrosis in contrast to the isolated use of these methods.

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Identification of lower-order inositol phosphates (IP5 and IP4) in soil extracts as determined by hypobromite oxidation and solution 31P NMR spectroscopy

10.5194/bg-2019-431 ◽

2019 ◽

Author(s):

Jolanda E. Reusser ◽

René Verel ◽

Daniel Zindel ◽

Emmanuel Frossard ◽

Timothy I. McLaren

Keyword(s):

Nmr Spectroscopy ◽

Lower Order ◽

31P Nmr ◽

Inositol Phosphates ◽

31P Nmr Spectroscopy ◽

Organic P ◽

Soil Extracts ◽

Broad Signal ◽

Solution 31P Nmr Spectroscopy ◽

First Time

Abstract. Inositol phosphates (IP) are a major pool of identifiable organic phosphorus (P) in soil. However, insight on their distribution and cycling in soil remains limited, particularly of lower-order IP (IP5 and IP4). This is because their quantification typically requires a series of chemical extractions, including hypobromite oxidation to isolate IP, followed by chromatographic separation. Here, for the first time, we identify the chemical nature of organic P in four soil extracts following hypobromite oxidation using solution 31P NMR spectroscopy and transverse relaxation (T2) experiments. Soil samples analysed include the A horizon of a Ferralsol from Colombia, of a Cambisol from Switzerland, of a Gleysol from Switzerland and of a Cambisol from Germany. Solution 31P NMR spectra of the phosphomonoester region on soil extracts following hypobromite oxidation revealed an increase in the number of sharp signals (up to 70), and an on average 2-fold decrease in the concentration of the broad signal compared to the untreated soil extracts. We identified the presence of four stereoisomers of IP6, four stereoisomers of IP5, and scyllo-IP4 (using solution 31P NMR spectroscopy). We also identified for the first time two isomers of myo-IP5 in soil extracts: myo-(1,2,4,5,6)-IP5 and myo-(1,3,4,5,6)-IP5. Concentrations of total IP ranged from 1.4 to 159.3 mg P/kgsoil across all soils, of which between 9 % and 50 % were comprised of lower-order IP. Furthermore, we found that the T2 times, which are considered to be inversely related to the tumbling of a molecule in solution and hence its molecular size, were significantly shorter for the underlying broad signal compared to the sharp signals (IP6) in soil extracts following hypobromite oxidation. In summary, we demonstrate the presence of a plethora of organic P compounds in soil extracts, largely attributed to IP of various order, and provide new insight on the chemical stability of complex forms of organic P associated with soil organic matter.

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Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus Phycomyces blakesleeanus

Archives of Biological Sciences ◽

10.2298/abs1402847s ◽

2014 ◽

Vol 66 (2) ◽

pp. 847-857

Author(s):

Marina Stanic ◽

M. Zivic ◽

Mirzeta Hadzibrahimovic ◽

Aleksandra Pajdic ◽

S. Krizak ◽

...

Keyword(s):

Nmr Spectroscopy ◽

31P Nmr ◽

31P Nmr Spectroscopy ◽

Antimycin A ◽

Phosphate Metabolism ◽

Phycomyces Blakesleeanus ◽

Tightly Coupled ◽

A Cell ◽

Inorganic Phosphates

The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxidase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by 31P NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.

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Quantitative Determination of Krill Oil Composition by ATR-FTIR, NIR, and 31P NMR Spectroscopy

10.21748/am20.144 ◽

2020 ◽

Author(s):

Ashraf Ismail ◽

Sanaz Molaye Moghaddam ◽

Jean-Pierre MetabanzoulouSarya Aziz ◽

Jacqueline Sedman ◽

Mazen Bahadi

Keyword(s):

Nmr Spectroscopy ◽

Quantitative Determination ◽

31P Nmr ◽

31P Nmr Spectroscopy ◽

Krill Oil ◽

Oil Composition

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Enzymatic decomposition of a locked nucleoside phosphoramidate monitored by 31P NMR spectroscopy

Collection Symposium Series ◽

10.1135/css201414356 ◽

2014 ◽

Author(s):

Eliška Procházková ◽

Hubert Hřebabecký ◽

Radim Nencka ◽

Martin Dračínský

Keyword(s):

Nmr Spectroscopy ◽

31P Nmr ◽

31P Nmr Spectroscopy

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Effect of Dietary Magnesium on Post Mortem Phosphocreatine Utilization in Skeletal Muscle of Swine: A Non-Invasive Study Using 31P-NMR Spectroscopy

Acta Veterinaria Scandinavica ◽

10.1186/bf03548184 ◽

1993 ◽

Vol 34 (4) ◽

pp. 397-404

Author(s):

B. Moesgaard ◽

I. Errebo Larsen ◽

B. Quistorff ◽

I. Therkelsen ◽

V. Grøsfjeld Christensen ◽

...

Keyword(s):

Skeletal Muscle ◽

Nmr Spectroscopy ◽

31P Nmr ◽

31P Nmr Spectroscopy ◽

Post Mortem ◽

Non Invasive ◽

Dietary Magnesium

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Unravelling the dissolution mechanism of polyphosphate glasses by 31P NMR spectroscopy: ligand competition and reactivity of intermediate complexes

Dalton Transactions ◽

10.1039/d0dt03381b ◽

2021 ◽

Author(s):

Dahiana Andrea Avila Salazar ◽

Peter Bellstedt ◽

Atsuhiro Miura ◽

Yuki Oi ◽

Toshihiro Kasuga ◽

...

Keyword(s):

Nuclear Magnetic Resonance ◽

Solid State ◽

Nmr Spectroscopy ◽

Biomedical Applications ◽

Structural Changes ◽

31P Nmr ◽

Dissolution Mechanism ◽

31P Nmr Spectroscopy ◽

Glass Dissolution ◽

Polyphosphate Glasses

Phosphate glass dissolution can be tailored via compositional and subsequent structural changes, which is of interest for biomedical applications such as therapeutic ion delivery. Here, solid-state 31P nuclear magnetic resonance...

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