scholarly journals Quantitative Analysis of the Cardiac Phosphoproteome in Response to Acute β-Adrenergic Receptor Stimulation In Vivo

2021 ◽  
Vol 22 (22) ◽  
pp. 12584
Author(s):  
Alican Güran ◽  
Yanlong Ji ◽  
Pan Fang ◽  
Kuan-Ting Pan ◽  
Henning Urlaub ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Adrenergic Receptor ◽  
Molecular Basis ◽  
Cell Signalling ◽  
Receptor Stimulation ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Major Mechanism ◽  
Signalling Network

β-adrenergic receptor (β-AR) stimulation represents a major mechanism of modulating cardiac output. In spite of its fundamental importance, its molecular basis on the level of cell signalling has not been characterised in detail yet. We employed mass spectrometry-based proteome and phosphoproteome analysis using SuperSILAC (spike-in stable isotope labelling by amino acids in cell culture) standardization to generate a comprehensive map of acute phosphoproteome changes in mice upon administration of isoprenaline (ISO), a synthetic β-AR agonist that targets both β1-AR and β2-AR subtypes. Our data describe 8597 quantitated phosphopeptides corresponding to 10,164 known and novel phospho-events from 2975 proteins. In total, 197 of these phospho-events showed significantly altered phosphorylation, indicating an intricate signalling network activated in response to β-AR stimulation. In addition, we unexpectedly detected significant cardiac expression and ISO-induced fragmentation of junctophilin-1, a junctophilin isoform hitherto only thought to be expressed in skeletal muscle. Data are available via ProteomeXchange with identifier PXD025569.

scholarly journals Stable isotope labelling in vivo as an aid to protein identification in peptide mass fingerprinting

PROTEOMICS ◽  
2002 ◽  
Vol 2 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Julie M. Pratt ◽  
Duncan H. L. Robertson ◽  
Simon J. Gaskell ◽  
Isabel Riba-Garcia ◽  
Simon J. Hubbard ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Protein Identification ◽  
Peptide Mass Fingerprinting ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Peptide Mass

scholarly journals Analytical Considerations of Stable Isotope Labelling in Lipidomics

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 151 ◽  
Author(s):  
Alexander Triebl ◽  
Markus Wenk
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Clinical Settings ◽  
Stable Isotope Labelling ◽  
Stable Isotope Label ◽  
Isotope Labelling ◽  
Isotope Label ◽  
Lipid Biomarker ◽  
Staple Technique ◽  
Biomarker Research

Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in clinical settings. Stable isotope labelling has become a staple technique in the analysis of small molecule metabolism and dynamics, as it is the only experimental setup by which biosynthesis, remodelling and degradation of biomolecules can be directly measured. Using state-of-the-art analytical technologies such as chromatography-coupled high resolution tandem mass spectrometry, the stable isotope label can be precisely localized and quantified within the biomolecules. The application of stable isotope labelling to lipidomics is however complicated by the diversity of lipids and the complexity of the necessary data analysis. This article discusses key experimental aspects of stable isotope labelling in the field of mass spectrometry-based lipidomics, summarizes current applications and provides an outlook on future developments and potential.

scholarly journals Dynamics of murine brain protein synthesis in vivo identify the hippocampus, cortex and cerebellum as highly active metabolic sites

2019 ◽  
Author(s):  
Ser Sue Ng ◽  
Jung Eun Park ◽  
Wei Meng ◽  
Christopher Li-Hsian Chen ◽  
Raj N. Kalaria ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Culture ◽  
Protein Synthesis ◽  
Stable Isotope ◽  
Density Lipoprotein ◽  
Apolipoprotein A1 ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Highly Active

AbstractIdentification of proteins that are synthesized de novo in response to specific microenvironmental cues is critical to understanding the molecular mechanisms that underpin key physiological processes and pathologies. Here we report that a brief period of pulsed SILAC diet (Stable Isotope Labelling by Amino acids in Cell culture) enables determination of biological functions corresponding to actively translating proteins in the mouse brain. Our data demonstrate that the hippocampus, cortex and cerebellum are highly active sites of protein synthesis, rapidly expressing key mediators of nutrient sensing and lipid metabolism, as well as critical regulators of synaptic function, axon guidance, and circadian entrainment. Together, these findings confirm that protein metabolic activity varies significantly between brain regions in vivo and indicate that pSILAC-based approaches can identify specific anatomical sites and biological pathways likely to be suitable for drug targeting in neurodegenerative disorders.AbbreviationsApoA1: Apolipoprotein A1, ApoA4: Apolipoprotein A4, ApoE: Apolipoprotein E, ApoJ/Clu: Apolipoprotein J/Clusterin, App: Amyloid-β precursor/A4 protein: App, HDL: high density lipoprotein, Lrp1: Low density lipoprotein receptor-related protein 1, pSILAC: pulsed SILAC, pSIVOM: pulsed-SILAC in vivo labelling in mouse, SILAC: Stable Isotope Labelling by Amino acids in Cell culture)

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2351-2355 ◽  
Author(s):  
Cunyu Yan ◽  
Jason W. Schmidberger ◽  
Fabio Parmeggiani ◽  
Shaneela A. Hussain ◽  
Nicholas J. Turner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
High Throughput ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Direct Infusion ◽  
Stable Isotope Labelling ◽  
Isotope Labelling

The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry enabled high-throughput and sensitive monitoring of biocatalytic reactions.

scholarly journals Natural isotope correction improves analysis of protein modification dynamics

2020 ◽  
Author(s):  
Jörn Dietze ◽  
Alienke van Pijkeren ◽  
Mathias Ziegler ◽  
Marcel Kwiatkowski ◽  
Ines Heiland
Keyword(s):  
Mass Spectrometry ◽  
Protein Modification ◽  
High Resolution Mass Spectrometry ◽  
Mass Spectrometry Data ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Naturally Occurring ◽  
Natural Isotope ◽  
Experimental Approaches ◽  
Correct Estimation

AbstractStable isotope labelling in combination with high resolution mass spectrometry approaches are increasingly used to analyse both metabolite and protein modification dynamics. To enable correct estimation of the resulting dynamics it is critical to correct the measured values for naturally occurring stable isotopes, a process commonly called isotopologue correction or deconvolution. While the importance of isotopologue correction is well recognized in metabolomics, it has received far less attention in proteomics approaches. Although several tools exist that enable isotopologue correction of mass spectrometry data, none of them is universally applicable for all potential experimental approaches. We here present PICor which has been streamlined for multiple isotope labelling isotopologue correction in proteomics or metabolomics approaches. We demonstrate the importance for accurate measurement of the dynamics of protein modifications, such as histone acetylation.

Stable isotope labelling of phosphatidylcholine synthesis in vivo in children with acute lung injury

2010 ◽  
Vol 95 (Suppl 1) ◽  
pp. A43.1-A43
Author(s):  
K Goss ◽  
L Ward ◽  
V Ledger ◽  
G Koster ◽  
P Townsend ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Stable Isotope ◽  
Lung Injury ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Phosphatidylcholine Synthesis

scholarly journals Ex vivo and in vivo stable isotope labelling of central carbon metabolism and related pathways with analysis by LC–MS/MS

2019 ◽  
Vol 14 (2) ◽  
pp. 313-330 ◽  
Author(s):  
Min Yuan ◽  
Daniel M. Kremer ◽  
He Huang ◽  
Susanne B. Breitkopf ◽  
Issam Ben-Sahra ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Carbon Metabolism ◽  
Ex Vivo ◽  
Central Carbon Metabolism ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Central Carbon
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