scholarly journals Functional Characterization of Aluminum (Al)-Responsive Membrane-Bound NAC Transcription Factors in Soybean Roots

2021 ◽  
Vol 22 (23) ◽  
pp. 12854
Author(s):  
Yan Lin ◽  
Guoxuan Liu ◽  
Yingbing Xue ◽  
Xueqiong Guo ◽  
Jikai Luo ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Functional Characterization ◽  
Duplicate Gene ◽  
Al Toxicity ◽  
Al Tolerance ◽  
Cell Wall Modification ◽  
Nac Transcription Factors ◽  
Al Stress ◽  
Membrane Bound

The membrane-bound NAC transcription (NTL) factors have been demonstrated to participate in the regulation of plant development and the responses to multiple environmental stresses. This study is aimed to functionally characterize soybean NTL transcription factors in response to Al-toxicity, which is largely uncharacterized. The qRT-PCR assays in the present study found that thirteen out of fifteen GmNTL genes in the soybean genome were up-regulated by Al toxicity. However, among the Al-up-regulated GmNTLs selected from six duplicate gene pairs, only overexpressing GmNTL1, GmNTL4, and GmNTL10 could confer Arabidopsis Al resistance. Further comprehensive functional characterization of GmNTL4 showed that the expression of this gene in response to Al stress depended on root tissues, as well as the Al concentration and period of Al treatment. Overexpression of GmNTL4 conferred Al tolerance of transgenic Arabidopsis in long-term (48 and 72 h) Al treatments. Moreover, RNA-seq assay identified 517 DEGs regulated by GmNTL4 in Arabidopsis responsive to Al stress, which included MATEs, ALMTs, PMEs, and XTHs. These results suggest that the function of GmNTLs in Al responses is divergent, and GmNTL4 might confer Al resistance partially by regulating the expression of genes involved in organic acid efflux and cell wall modification.

Functional characterization of tomato membrane-bound NAC transcription factors

2016 ◽  
Vol 93 (4-5) ◽  
pp. 511-532 ◽  
Author(s):  
Payel Bhattacharjee ◽  
Rohit Das ◽  
Arunava Mandal ◽  
Pallob Kundu
Keyword(s):  
Transcription Factors ◽  
Functional Characterization ◽  
Nac Transcription Factors ◽  
Membrane Bound

Functional characterization of two bZIP transcription factors in Verticillium dahliae

Gene ◽  
2017 ◽  
Vol 626 ◽  
pp. 386-394 ◽  
Author(s):  
Yulin Fang ◽  
Dianguang Xiong ◽  
Longyan Tian ◽  
Chen Tang ◽  
Yonglin Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Verticillium Dahliae ◽  
Functional Characterization ◽  
Bzip Transcription Factors

scholarly journals Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent

2019 ◽  
Author(s):  
Sonia Balyan ◽  
Sombir Rao ◽  
Sarita Jha ◽  
Chandni Bansal ◽  
Jaishri Rubina Das ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Functional Characterization ◽  
Transcriptome Profiling ◽  
Negative Regulator ◽  
Comparative Transcriptome ◽  
Regulatory Pathways ◽  
Cool Region ◽  
Promoter Swapping

AbstractThe footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub-tropics. Knowledge of novel regulatory hot-spots from varieties growing in the Indian sub-continent climatic zones could be vital for developing HS-resilient crops. Comparative transcriptome-wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar-pair short-listed from a pool of varieties exhibiting variable thermo-sensitivity using physiological, survival and yield-related traits revealed redundant to cultivar-specific HS-regulation with more up-regulated genes for CLN1621L than CA4. The anatgonisiticly-expressing genes include enzymes; have roles in plant defense and response to different abiotic stresses. Functional characterization of three antagonistic genes by overexpression and TRV-VIGS silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis), that are up-regulated in tolerant cultivar, as positive regulators of HS-tolerance and Solyc03g020030 (Pin-II proteinase inhibitor), that is down-regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress-responsive cis-elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar-specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS-tolerance in tomato. This study identifies several novel HS-tolerance genes and provides proof of their utility in tomato-thermotolerance.HighlightNovel heat stress regulatory pathways uncovered by comparative transcriptome profiling between contrasting tomato cultivars from Indian sub-continent for improving thermotolerance. (20/30)

Transcription factors–DNA interactions in rice: identification and verification

2019 ◽  
Vol 21 (3) ◽  
pp. 946-956 ◽  
Author(s):  
Zijie Shen ◽  
Yuan Lin ◽  
Quan Zou
Keyword(s):  
Transcription Factors ◽  
Functional Characterization ◽  
Rice Genome ◽  
Research Strategies ◽  
Promoter Regions ◽  
Rice Varieties ◽  
Rice Genome Sequence ◽  
Advantages And Disadvantages ◽  
Genomics Research

Abstract The completion of the rice genome sequence paved the way for rice functional genomics research. Additionally, the functional characterization of transcription factors is currently a popular and crucial objective among researchers. Transcription factors are one of the groups of proteins that bind to either enhancer or promoter regions of genes to regulate expression. On the basis of several typical examples of transcription factor analyses, we herein summarize selected research strategies and methods and introduce their advantages and disadvantages. This review may provide some theoretical and technical guidelines for future investigations of transcription factors, which may be helpful to develop new rice varieties with ideal traits.

scholarly journals Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN

Science ◽  
2018 ◽  
Vol 362 (6414) ◽  
pp. eaat0572 ◽  
Author(s):  
Quinlan L. Sievers ◽  
Georg Petzold ◽  
Richard D. Bunker ◽  
Aline Renneville ◽  
Mikołaj Słabicki ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Small Molecules ◽  
Zinc Finger ◽  
Ubiquitin Ligase ◽  
Biochemical Analysis ◽  
Zinc Fingers ◽  
Functional Characterization ◽  
C2h2 Zinc Finger

The small molecules thalidomide, lenalidomide, and pomalidomide induce the ubiquitination and proteasomal degradation of the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3) by recruiting a Cys2-His2 (C2H2) zinc finger domain to Cereblon (CRBN), the substrate receptor of the CRL4CRBN E3 ubiquitin ligase. We screened the human C2H2 zinc finger proteome for degradation in the presence of thalidomide analogs, identifying 11 zinc finger degrons. Structural and functional characterization of the C2H2 zinc finger degrons demonstrates how diverse zinc finger domains bind the permissive drug-CRBN interface. Computational zinc finger docking and biochemical analysis predict that more than 150 zinc fingers bind the drug-CRBN complex in vitro, and we show that selective zinc finger degradation can be achieved through compound modifications. Our results provide a rationale for therapeutically targeting transcription factors that were previously considered undruggable.

scholarly journals Deep expression scrutiny of juxtaposed wild and cultivated lentil furnishes new insight into aluminium tolerance mechanism

2020 ◽  
Author(s):  
Dharmendra Singh ◽  
Chandan Kumar Singh ◽  
Jyoti Taunk ◽  
Ram Sewak Singh Tomar ◽  
Madan Pal ◽  
...  
Keyword(s):  
Pathway Analysis ◽  
De Novo ◽  
Crop Improvement ◽  
Al Toxicity ◽  
Aluminium Tolerance ◽  
Regulation Of Transcription ◽  
Al Tolerance ◽  
Illumina Hiseq ◽  
Al Stress ◽  
Protein Ubiquitination

Abstract Background: Aluminium (Al) stress hinders crop productivity in acidic soils. Lentil contains rich source of protein and micronutrients and cultivated in different parts of world. To enhance knowledge about Al toxicity tolerance, present study emphasizes on mechanistic analysis of genes associated with Al stress through de novo transcriptomic analysis of tolerant (L-4602), wild (ILWL-15) and sensitive (BM-4) genotypes. Result: Illumina HiSeq 2500 platform evaluated contigs ranging from 15,305 to 18,861 for all the samples with N 50 values of 1795 bp. Four annotation softwares revealed differential regulation of several genes where 30,158 genes were specifically up-regulated for combinations under Al stress conditions alone. Top up-regulated Differentially Expressed Genes (DEGs) in tolerant cultivar when compared to the sensitive one were found to be involved in protein transport as well as degradation, defences, cell growth and development. Wild v/s cultivar comparison revealed upregulation of wild DEGs that are involved in regulation of transcription in differentiating cells, pre-mRNA splicing, catalysis and protein ubiquitination. Based on assembled Unigenes, 89,722 high-quality SNPs and 39,874 SSRs were detected. Twelve selected genes were validated using qRT-PCR. KEGG pathway analysis extracted 8,757 GO annotation terms within molecular, cellular and biological processes. Pathway analysis indicated that organic acid synthesis and their transportation along with detoxification of ROS, an alternate pathway involving metacaspase-1,4,9 for programmed cell death were also significantly induced due to Al stress. Conclusion: Present study unveils the characterization of differential transcripts generated under Al stress indicating Al tolerance as a multiplex phenomenon which will directly widen crop improvement programmes for Al toxicity utilizing molecular approaches.

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