scholarly journals Preventative Approach to Microbial Control of Capnodis tenebrionis by Soil Application of Metarhizium brunneum and Beauveria bassiana

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 319
Author(s):  
Dana Ment ◽  
Hysen Kokiçi ◽  
Enrico de Lillo
Keyword(s):  
Beauveria Bassiana ◽  
Microbial Control ◽  
Soil Surface ◽  
Field Application ◽  
Post Inoculation ◽  
Metarhizium Brunneum ◽  
Root Localization ◽  
Neonate Larvae ◽  
Preventative Approach ◽  
Colonization Rates

Management of the Mediterranean flat-headed root-borer, Capnodis tenebrionis, is critical due to the larvae’s root localization. Neonate larvae can be exposed to natural enemies before penetrating the roots. Application of Metarhizium brunneum strain Mb7 and Beauveria bassiana strain GHA formulations on rice granules was investigated for their efficacy against C. tenebrionis larvae. Mb7 application, evaluated on apricot twigs, significantly and dose-dependently reduced colonization rates of neonates, with highest mortality at 108 conidia/g soil. Neonate susceptibility to Mb7 and GHA was evaluated on potted rootstocks (GF677 almond × peach, 2729 plum) planted in entomopathogenic fungi (EPF)-premixed soil (1.3–1.6 × 105 conidia/cm3 soil) or in EPF-free soil surface-treated with 5 g Mb7 fungal granules (1.25 × 109 conidia). Larval colonization rates were reduced 7.4-fold in 2729 by both fungi; only Mb7 completely prevented colonization of GF677 by larvae. Larvae inside plant galleries exhibited mycosis with EPF-treated soils and both fungi proliferated on larval frass. Mb7 conidia germinated in the rhizosphere of GF677, and conidia of both fungi remained viable throughout the trial. Galleria baiting technique was used on EPF-treated soil to evaluate EPF infectivity over time; Mb7 and GHA persisted 180 and 90 days post inoculation, respectively. The formulation (fungus-covered rice grains), delivery method (mixing with soil) and persistence (3–6 months) of Mb7 and GHA are feasible for potential field application to control C. tenebrionis.

Experimental parasitization of larvae of the gypsy moth, Porthetria dispar (L.), with the entomogenous fungus, Beauveria bassiana (Balsamo) Vuill

Parasitology ◽  
1975 ◽  
Vol 70 (3) ◽  
pp. 341-346 ◽  
Author(s):  
S. S. Wasti ◽  
G. C. Hartmann
Keyword(s):  
Beauveria Bassiana ◽  
Gypsy Moth ◽  
Post Inoculation ◽  
Entomogenous Fungus ◽  
Porthetria Dispar ◽  
Host Penetration ◽  
Fungus Beauveria Bassiana ◽  
Wax Moth

Second-instar larvae of the gypsy moth, Porthetria dispar (L.) were infected with Beauveria bassiana (Balsamo) Vuill. by allowing larvae to crawl over sporulating cultures of the fungus. Virulence of the pathogen was increased by inoculating larvae of the wax moth, Galleria melonella (L.), twice in serial succession. Observations were made on the infectivity and histopathology of the parasite in the host. Penetration of the cuticle occurred 24 h after inoculation and at 64 h post-inoculation the interior was completely filled with hyphae. Penetration of the gut wall was also observed.

scholarly journals Compatibility between the entomopathogenic fungus Beauveria bassiana and insecticides used in coffee plantations

2003 ◽  
Vol 60 (4) ◽  
pp. 663-667 ◽  
Author(s):  
Carolina Natali de Oliveira ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
Lídio Sueki Kawazoe
Keyword(s):  
Beauveria Bassiana ◽  
Vegetative Growth ◽  
Entomopathogenic Fungus ◽  
Microbial Control ◽  
Infection Process ◽  
Conservation Strategy ◽  
Coffee Plantations ◽  
Conidia Germination ◽  
Fungus Beauveria Bassiana

Microbial control in integrated pest management (IPM) programs of coffee plantations is an important factor for the reduction of pest population densities. The use of selective pesticides can be associated with entomopathogens, increasing the efficiency of the control and reducing the use of required insecticides. The in vitro fungitoxic effect of insecticide formulations of Thiamethoxam, Cyfluthrin, Deltamethrin, Alpha-Cypermethrin, Triazophos, Chlorpyrifos, Fenpropathrin and Endosulfan and Beauveria bassiana (CG 425 strain) was evaluated at three concentrations (FR = average field recommendation; 0.5 ´ FR and 2 ´ FR). Effects of these products on conidia germination, vegetative growth and sporulation were compared. Only five insecticides, at the FR concentration, promoted conidia viability higher than 60%. Viability should be considered the most important factor to be evaluated since it is the first step of the infection process. The insecticide formulations of Alpha-Cypermethrin, Thiamethoxam and Cyfluthrin caused the lower inhibition level on conidia germination at the two lower concentrations, with no difference in relation to the control. With respect to vegetative growth analysis, Thiamethoxam at the two lower concentrations was not found to cause radial growth inhibition. Thiamethoxam caused the smallest inhibition level with regard to conidia production. The use of Alpha-Cypermethrin and Thiamethoxam formulations in coffee IPM programs for a B. bassiana inoculum conservation strategy are recommended, since these products were compatible with the entomopathogenic fungus Beauveria bassiana (CG 425), an important natural control agent of the coffee berry borer, Hypothenemus hampei.

scholarly journals Existence of entomopathogen fungi, Beauveria bassiana as an endophyte in cacao seedlings

2015 ◽  
Vol 31 (3) ◽  
pp. 186-195
Author(s):  
Endang Sulistyowati ◽  
Febrilia Nur AINI
Keyword(s):  
Beauveria Bassiana ◽  
Plant Protection ◽  
Biological Control Agent ◽  
Block Design ◽  
Control Agent ◽  
Post Inoculation ◽  
Pod Borer ◽  
Fungal Entomopathogens ◽  
Randomized Complete Block Design ◽  
Fungal Entomopathogen

Beauveria bassiana is one of the entomopathogen fungi which is known as biological control agent of cocoa pod borer and cocoa mirids (Helopeltis spp.). Because of its effectiveness in the fields is still not consistent, so we conduct a research with the objective to know the possibility of Beauveria bassiana to be established as a endophyte. Various fungal entomopathogens have already been reported as endophytes and the various methods used to inoculate the plants with B. bassiana were partially effective. The research has been conducted in laboratory of Plant Protection, Indonesian Coffee and Cocoa Research Institute by inoculating of cocoa seeds and cocoa nursery with B. bassiana suspension.  The trial was arranged  by randomized complete block design with a factorial arrangement. The factor were spore concentration of B. bassiana (0; 2; and 4 g/ 10 l) and cocoa varieties (family of ICS 60, TSH858, and hybrid). The trial were use  four replications. The results showed that the fungal entomopathogen B. bassiana was established as an endophyte in cocoa seedling, both from cocoa seeds and nursery application. Percentage of existence of B. bassiana colonies as endophytes one month after seeds application were ICS 60 amounted to 93.3 % both on concentration treatments, while the families of TSH 858 by 80 % and 86.67 % respectively in 2 g and 4 g per 10 l of B. bassiana spores concentration treament.. The lowest percentage was in hybrids, which amounted to 66.67% and 50%. B. bassiana colonies was exixtence as an endophyte in culture from root, stem and leaves of cocoa seedling up to 5 months post inoculation. While the application on nursery by soil drenshing, leaf spraying, and stem injection , it was known that B. bassiana colonies were found in the tissues of leaves, stems, and roots until two months after application. Colonies of B. bassiana as endophytes still exsist until six weeks after nursery was planted in the field. 

The entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana promote systemic immunity and confer resistance to a broad range of pests and pathogens in tomato

2021 ◽  
Author(s):  
Rupali Gupta ◽  
Ravindran Keppanan ◽  
Meirav Leibman-Markus ◽  
Dalia Rav David ◽  
Yigal Elad ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plant Pathogens ◽  
Plant Immunity ◽  
Systemic Immunity ◽  
Metarhizium Brunneum ◽  
Promote Plant Growth ◽  
Arthropod Pests

Biocontrol agents can control pathogens by re-enforcing systemic plant resistance through systemic acquired resistance (SAR) or induced systemic resistance (ISR). Trichoderma spp. can activate the plant immune system through ISR, priming molecular mechanisms of defense against pathogens. Entomopathogenic fungi (EPF) can infect a wide range of arthropod pests, and play an important role in reducing pests' population. Here, we investigated the mechanisms by which EPF control plant diseases. We tested two well studied EPF, Metarhizium brunneum isolate Mb7 and Beauveria bassiana as the commercial product Velifer, for their ability to induce systemic immunity and disease resistance against several fungal and bacterial phytopathogens, and their ability to promote plant growth. We compared the activity of these EPF to an established biocontrol agent, T. harzianum T39, a known inducer of systemic plant immunity and broad disease resistance. The three fungal agents were effective against several fungal and bacterial plant pathogens and arthropod pests. Our results indicate that EPF induce systemic plant immunity and disease resistance by activating the plant host defense machinery, as evidenced by increases in reactive oxygen species (ROS) production and defense gene expression, and that EPF promote plant growth. EPF should be considered as control means for Tuta absoluta. We demonstrate that, with some exceptions, biocontrol in tomato can be equally potent by the tested EPF and T. harzianum T39, against both insect pests and plant pathogens. Taken together, our findings suggest that EPF may find use in broad-spectrum pest and disease management and as plant growth promoting agents.

scholarly journals Effects of Seedling Age on Colonization Patterns of Citrus limon Plants by Endophytic Beauveria bassiana and Metarhizium anisopliae and Their Influence on Seedlings Growth

2020 ◽  
Vol 6 (1) ◽  
pp. 29
Author(s):  
Bamisope Steve Bamisile ◽  
Komivi Senyo Akutse ◽  
Chandra Kanta Dash ◽  
Muhammad Qasim ◽  
Luis Carlos Ramos Aguila ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Physiological Status ◽  
Citrus Limon ◽  
Post Inoculation ◽  
Seedling Age ◽  
Fungal Strains ◽  
Fungal Entomopathogens ◽  
Colonization Success ◽  
Colonization Patterns

The inoculation methods, the fungal strains, and several other factors are known to influent the success of fungal entomopathogens colonization in plants. The physiological status of the plant could also be another determinant. In the present study, the ability of three strains of Beauveria bassiana and one strain of Metarhizium anisopliae to successfully colonize Citrus limon plants and the influence of seedling age on endophytic colonization success was examined. Three, 4, and 6 months old seedlings were inoculated with 10 mL of 1 × 108 conidial·mL−1 suspensions of each of the four fungal strains via foliar spraying. All fungal strains successfully colonized citrus seedlings and were sustained up to 2 months in colonized plants irrespective of the seedling age, with differences in the mean percentage colonization recorded at various post-inoculation periods among the fungal strains. The highest percent endophytic fungi recovery rate was recorded in the 3 months old seedlings, where fungal mycelia of inoculated fungi were successfully re-isolated from 65.6% of the untreated newly developed leaf and stem tissues. One strain of B. bassiana, BB Fafu-12, significantly improved seedling height and leaf number. The study demonstrates the influence of seedling age on B. bassiana and M. anisopliae successful colonization in the citrus plant.

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