The entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana promote systemic immunity and confer resistance to a broad range of pests and pathogens in tomato

2021 ◽  
Author(s):  
Rupali Gupta ◽  
Ravindran Keppanan ◽  
Meirav Leibman-Markus ◽  
Dalia Rav David ◽  
Yigal Elad ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plant Pathogens ◽  
Plant Immunity ◽  
Systemic Immunity ◽  
Metarhizium Brunneum ◽  
Promote Plant Growth ◽  
Arthropod Pests

Biocontrol agents can control pathogens by re-enforcing systemic plant resistance through systemic acquired resistance (SAR) or induced systemic resistance (ISR). Trichoderma spp. can activate the plant immune system through ISR, priming molecular mechanisms of defense against pathogens. Entomopathogenic fungi (EPF) can infect a wide range of arthropod pests, and play an important role in reducing pests' population. Here, we investigated the mechanisms by which EPF control plant diseases. We tested two well studied EPF, Metarhizium brunneum isolate Mb7 and Beauveria bassiana as the commercial product Velifer, for their ability to induce systemic immunity and disease resistance against several fungal and bacterial phytopathogens, and their ability to promote plant growth. We compared the activity of these EPF to an established biocontrol agent, T. harzianum T39, a known inducer of systemic plant immunity and broad disease resistance. The three fungal agents were effective against several fungal and bacterial plant pathogens and arthropod pests. Our results indicate that EPF induce systemic plant immunity and disease resistance by activating the plant host defense machinery, as evidenced by increases in reactive oxygen species (ROS) production and defense gene expression, and that EPF promote plant growth. EPF should be considered as control means for Tuta absoluta. We demonstrate that, with some exceptions, biocontrol in tomato can be equally potent by the tested EPF and T. harzianum T39, against both insect pests and plant pathogens. Taken together, our findings suggest that EPF may find use in broad-spectrum pest and disease management and as plant growth promoting agents.

scholarly journals Actinomycetes, promising tools to control plant diseases and to promote plant growth

2005 ◽  
Vol 82 (3) ◽  
pp. 85-102 ◽  
Author(s):  
C.L. Doumbou ◽  
M.K. Hamby Salove ◽  
D.L. Crawford ◽  
C. Beaulieu
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Soil Microbial Biomass ◽  
Extracellular Enzymes ◽  
Control Plant ◽  
Plant Diseases ◽  
Soil Microbial ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Actinomycetes represent a high proportion of the soil microbial biomass and have the capacity to produce a wide variety of antibiotics and of extracellular enzymes. Several strains of actinomycetes have been found to protect plants against plant diseases. This review focuses on the potential of actinomycetes as (a) source of agroactive compounds, (b) plant growth promoting organisms, and (c) biocontrol tools of plant diseases. This review also addresses examples of biological control of fungal and bacterial plant pathogens by actinomycetes species which have already reached the market or are likely to be exploited commercially within the next few years.

scholarly journals A Community Effort: Combining Functional Amplicon Sequencing and Metagenomics Reveals Potential Biosynthetic Gene Clusters Associated with Protective Phenotypes in Rhizosphere Microbiomes

mSystems ◽  
2021 ◽  
Author(s):  
Jaclyn M. Winter
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Gene Clusters ◽  
Amplicon Sequencing ◽  
Plant Roots ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
First Line ◽  
Community Effort ◽  
Promote Plant Growth

Soil-dwelling microorganisms associated with plant roots carry out essential processes that promote plant growth and productivity. In addition to these beneficial functions, the rhizosphere microbiome also serves as the first line of defense against many plant pathogens.

Redefining the dose of the entomopathogenic fungus Metarhizium brunneum (Ascomycota, Hypocreales) to increase Fe bioavailability and promote plant growth in calcareous and sandy soils

2017 ◽  
Vol 418 (1-2) ◽  
pp. 387-404 ◽  
Author(s):  
Silvia Raya–Díaz ◽  
Enrique Quesada–Moraga ◽  
Vidal Barrón ◽  
María Carmen del Campillo ◽  
Antonio Rafael Sánchez–Rodríguez
Keyword(s):  
Plant Growth ◽  
Entomopathogenic Fungus ◽  
Sandy Soils ◽  
Metarhizium Brunneum ◽  
Promote Plant Growth

scholarly journals Application of Riau local entomopathogen fungi Beauveria bassiana toward Spodoptera litura pest and plants growth red chili

2021 ◽  
Vol 3 (1) ◽  
pp. 130-135
Author(s):  
Hapsoh ◽  
Desita Salbiah ◽  
Isna Rahma Dini ◽  
Johanna Yosefin BR Tobing
Keyword(s):  
Plant Growth ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Spodoptera Litura ◽  
Raw Material ◽  
Horticultural Crop ◽  
Randomized Design ◽  
Plant Pest ◽  
Completely Randomized Design ◽  
Experimental Garden

Red chili is a horticultural crop that is widely cultivated commercially. The need for red chili is increasing, in accordance with the increasing variety of types of food that use red chili as a raw material. Pest attacks on red chili plants are an obstacle in red chili cultivation. Spodoptera litura is a red chili pest that can cause yield reductions of up to 80%. Control using the Riau local entomopathogenic fungi Beauveria bassiana is an effort to control S. litura using environmentally friendly materials. This study aims to determine the effect of the application of the Riau local entomopathogenic fungi B. bassiana to contol S. litura pests and the growth of red chili plants. The research was carried out at the Plant Pest Laboratory and Experimental Garden, Faculty of Agriculture, Riau University from July to August 2020. This study was designed in a completely randomized design with five treatments, namely the concentration of Riau local Riau B. bassiana 20 gl-1 water, 25 gl-1 water, 30 gl-1 water, 35 gl-1 water and 40 gl-1 water and four replications to obtain 20 experimental units. The results showed that the concentration of Riau local B. bassiana 30 gl-1 of water containing a conidia density of 4.1 × 108 con.ml-1 was a concentration that affected S. litura pests and chili plant growth by causing the intensity of S. litura pests which could be categorized as lower at 4.15% and can cause a peak in larval death on the fourth day after application.

Paecilomyces variotii extracts (ZNC) enhance plant immunity and promote plant growth

2019 ◽  
Vol 441 (1-2) ◽  
pp. 383-397 ◽  
Author(s):  
Chongchong Lu ◽  
Haifeng Liu ◽  
Depeng Jiang ◽  
Lulu Wang ◽  
Yanke Jiang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Immunity ◽  
Paecilomyces Variotii ◽  
Promote Plant Growth

scholarly journals Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

2020 ◽  
Author(s):  
Corné M. J. Pieterse ◽  
Roeland L. Berendsen ◽  
Ronnie de Jonge ◽  
Ioannis A. Stringlis ◽  
Anja J. H. Van Dijken ◽  
...  
Keyword(s):  
Plant Growth ◽  
Molecular Mechanisms ◽  
Root Colonization ◽  
Dianthus Caryophyllus ◽  
Systemic Resistance ◽  
Systemic Immunity ◽  
Microbe Interactions ◽  
Promote Plant Growth ◽  
Root Microbiome ◽  
Take All

Abstract Background Since the 1980s, numerous mutualistic Pseudomonas spp. strains have been used in studies on the biology of plant growth-promoting rhizobacteria (PGPR) and their interactions with host plants. In 1988, a strain from the Pseudomonas fluorescens group, WCS417, was isolated from lesions of wheat roots growing in a take-all disease-suppressive soil. In subsequent trials, WCS417 limited the build-up of take-all disease in field-grown wheat and significantly increased wheat yield. In 1991, WCS417 was featured in one of the first landmark studies on rhizobacteria-induced systemic resistance (ISR), in which it was shown to confer systemic immunity in carnation (Dianthus caryophyllus) against Fusarium wilt. The discovery that WCS417 conferred systemic immunity in the model plant species Arabidopsis thaliana in 1996 incited intensive research on the molecular mechanisms by which PGPR promote plant growth and induce broad-spectrum disease resistance in plants. Since then, the strain name appeared in over 750 studies on beneficial plant-microbe interactions. Scope In this review, we will highlight key discoveries in plant-microbe interactions research that have emerged from over 30 years of research featuring WCS417 as a model rhizobacterial strain. WCS417 was instrumental in improving our understanding of the microbial determinants that are involved in root colonization and the establishment of mutually beneficial interactions with the host plant. The model strain also provided novel insight into the molecular mechanisms of plant growth promotion and the onset and expression of rhizobacteria-ISR. More recently, WCS417 has been featured in studies on host immune evasion during root colonization, and chemical communication in the rhizosphere during root microbiome assembly. Conclusions Numerous studies on the modes of action of WCS417 have provided major conceptual advances in our understanding of how free-living mutualists colonize the rhizosphere, modulate plant immunity, and promote plant growth. The concepts may prove useful in our understanding of the molecular mechanisms involved in other binary plant-beneficial microbe interactions, and in more complex microbial community contexts, such as the root microbiome.

scholarly journals INDIGENOUS RHIZOBACTERIA SCREENING FROM TOMATO TO CONTROL Ralstonia syzigii subsp. indonesiensis AND PROMOTE PLANT GROWTH RATE AND YIELD

2019 ◽  
Vol 18 (2) ◽  
pp. 177
Author(s):  
Yulmira Yanti ◽  
Hasmiandy Hamid ◽  
Reflin Reflin
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Bacterial Wilt ◽  
Plant Pathogens ◽  
Wilt Disease ◽  
Growth Enhancement ◽  
Bacterial Wilt Disease ◽  
Promote Plant Growth ◽  
Phosphate Solubilizing ◽  
Selection Of

Indigenous rhizobacteria screening from tomato to control Ralstonia syzigii subsp. indonesiensis and promote plant growth rate and yield. Bacterial wilt is the most damaging vascular pathogen on tomato and many other crops in tropical, subtropical and warm temperate areas of the world which limits the production. Rhizobacteria have been concerned as potential biological control agents due to their ability to promote plant growth and health, and their role as antagonists of plant pathogens. The purpose of this research was to screen the best indigenous rhizobacteria (IRB) that able to control bacterial wilt disease and increase growth rate and yield of tomato plant. This research was conducted in 3 stages: (1) Isolation and selection of indigenous rhizobacteria as PGPR on tomato seedlings, consisted of 27 IRB isolates and a control, with triplications; (2) Selection of IRB isolates that control R. syzigii subsp. indonesiensis on tomato plants, which consisted of 8 treatments including 7 IRB and a control with 5 replications; (3) Characterization of IRB isolates ability to promote plant growth (indicated with IAA production & phosphate solubilizing). The variables observed were disease development, growth enhancement and IRB isolate ability to produce IAA and solubilize phosphate. The results showed that all IRB isolates were able to control bacterial wilt disease and increase the growth rate and yield of tomato. IR2.3.5, IR1.3.4 and IR1.4.2 were the best isolates in controlling R. syzigii subsp. indonesiensis and increasing the growth rate and yield by 81.25% and 68.72% respectively. All isolates showed various abilities to produce IAA, however, only isolates IR2.3.5 and IR1.3.4 that had abilities to solubilize phosphate.

scholarly journals Recessive Mutation Identifies Auxin-Repressed Protein ARP1, Which Regulates Growth and Disease Resistance in Tobacco

2014 ◽  
Vol 27 (7) ◽  
pp. 638-654 ◽  
Author(s):  
Yanying Zhao ◽  
Cheng Li ◽  
Jun Ge ◽  
Manyu Xu ◽  
Qian Zhu ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Plant Immunity ◽  
Genetic Locus ◽  
Recessive Mutation ◽  
Growth Enhancement ◽  
Auxin Response Factor Gene ◽  
Defense Response Genes ◽  
Growth Promoting ◽  
Molecular Patterns

To study the molecular mechanism that underpins crosstalk between plant growth and disease resistance, we performed a mutant screening on tobacco and created a recessive mutation that caused the phenotype of growth enhancement and resistance impairment (geri1). In the geri1 mutant, growth enhancement accompanies promoted expression of growth-promoting genes, whereas repressed expression of defense response genes is consistent with impaired resistance to diseases caused by viral, bacterial, and oomycete pathogens. The geri1 allele identifies a single genetic locus hypothetically containing the tagged GERI1 gene. The isolated GERI1 gene was predicted to encode auxin-repressed protein ARP1, which was determined to be 13.5 kDa in size. The ARP1/GERI1 gene was further characterized as a repressor of plant growth and an activator of disease resistance based on genetic complementation, gene silencing, and overexpression analyses. ARP1/GERI1 resembles pathogen-associated molecular patterns and is required for them to repress plant growth and activate plant immunity responses. ARP1/GERI1 represses growth by inhibiting the expression of AUXIN RESPONSE FACTOR gene ARF8, and ARP1/GERI1 recruits the NPR1 gene, which is essential for the salicylic-acid-mediated defense, to coregulate disease resistance. In conclusion, ARP1/GERI1 is an integral regulator for crosstalk between growth and disease resistance in the plant.

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