scholarly journals Trends of Lipophilic, Antioxidant and Hematological Parameters Associated with Conventional and Electronic Smoking Habits in Middle-Age Romanians

2019 ◽  
Vol 8 (5) ◽  
pp. 665 ◽  
Author(s):  
Mihaela Badea ◽  
Laura Gaman ◽  
Corina Delia ◽  
Anca Ilea ◽  
Florin Leașu ◽  
...  
Keyword(s):  
Vitamin A ◽  
Blood Cell ◽  
Antioxidant Status ◽  
Statistical Difference ◽  
Ldl Cholesterol ◽  
Electronic Cigarettes ◽  
Group Versus ◽  
Low Density Lipoproteins ◽  
Bioactive Molecules ◽  
Low Density

It is known that cigarette smoking is correlated with medical associated inquires. New electronic cigarettes are intensively advertised as an alternative to conventional smoking, but only a few studies demonstrate their harmful potential. A cross-sectional study was designed using 150 subjects from Brasov (Romania), divided into three groups: non-smokers (NS = 58), conventional cigarettes smokers (CS = 58) and electronic cigarettes users (ECS = 34). The aim of this study was to determine levels of some plasma lipophilic and hematological components, and the total antioxidant status that could be associated with the smoking status of the subjects. Serum low density lipoproteins (LDL) cholesterol increased significantly for ECS participants versus NS group (18.9% difference) (p < 0.05). Also, the CS group is characterized by an increase of serum LDL cholesterol (7.9% difference vs. NS), but with no significant statistical difference. The variation of median values of serum very low density lipoproteins (VLDL) was in order NS < ECS < CS, with statistical difference between NS and CS groups (34.6% difference; p = 0.023). When comparing the antioxidant status of the three groups, significant differences (p < 0.05) were obtained between NS vs. CS and NS vs. ECS. Similar behavior was identified for CS and ECS. Statistically significant changes (p < 0.0001) for both vitamin A and vitamin E were identified in the blood of NS vs. CS and NS vs. ECS, and also when comparing vitamin A in the blood of the CS group versus the ECS group (p < 0.05). When all groups were compared, the difference in the white blood cell (WBC) was (p = 0.008). A slight increase in the red blood cell (RBC) count was observed, but with no statistical difference between groups. These results indicated that conventional cigarette and e-cigarette usage promotes the production of excess reactive oxygen species, involving different pathways, different antioxidants and bioactive molecules.

Comparison of methods for measurement of apolipoprotein B and cholesterol in low-density lipoproteins

1997 ◽  
Vol 43 (2) ◽  
pp. 390-393 ◽  
Author(s):  
Ljubica Vrga ◽  
Christine Contacos ◽  
Stephen C H Li ◽  
David R Sullivan
Keyword(s):  
Apolipoprotein B ◽  
Ldl Cholesterol ◽  
Low Density Lipoproteins ◽  
Plasma Triglyceride ◽  
Low Density ◽  
Comparison Of Methods ◽  
Apo B ◽  
Simple Alternative ◽  
Cholesterol Measurement ◽  
Commercial Kit

Abstract We describe a new method for the direct measurement of LDL-apolipoprotein (apo) B by using a commercial kit that isolates LDL by immunoseparation. We evaluated immunoseparation of LDL for apo B and cholesterol measurement in 46 dyslipidemic patients with LDL-cholesterol (LDL-C) between 1.5 and 8.2 mmol/L, 11 of whom had plasma triglyceride (TG) concentrations &gt;4.0 mmol/L. There was a reasonable correlation (r = 0.94, n = 40) between LDL-apo B obtained after immunoseparation and d &gt;1.006 kg/L apo B obtained after ultracentrifugation. LDL-C by the immunoseparation method also correlated well (r = 0.98, n = 46) with the d &gt;1.006 kg/L cholesterol after ultracentrifugation. These results show that immunoseparation can be used to determine LDL-apo B, even in hypertriglyceridemic samples. This method may provide a quick and simple alternative for the identification of hyperapobetalipoproteinemia, even when TG concentrations are high.

Increased Lipid Peroxidation in Serum and Low-Density Lipoproteins Associated with Aging in Humans

2002 ◽  
Vol 72 (5) ◽  
pp. 315-320 ◽  
Author(s):  
Jale Balkan ◽  
Öznur Kanbagli ◽  
Güldal Mehmetçik ◽  
Ümit Mutlu-Türkoglu ◽  
Gülçin Aykaç-Toker ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin E ◽  
Ldl Cholesterol ◽  
Serum Vitamin ◽  
Lipid Peroxide ◽  
Low Density Lipoproteins ◽  
Elderly Subjects ◽  
Low Density ◽  
Diene Conjugates ◽  
Diene Conjugate

This study was carried out in 140 healthy subjects who were divided into three subgroups of age: young (21–40 years), mature (41–60 years), and elderly ( 61–85 years) to investigate lipid peroxides and the antioxidant system in serum and low-density lipoproteins (LDL). Serum levels of cholesterol and LDL-cholesterol increased with age. The elderly group was found to have higher polyunsaturated fatty acid (PUFA) levels, thiobarbituric acid reactive substances (TBARS), diene conjugates, and lower cholesterol-adjusted vitamin E levels and antioxidant activity (AOA) as compared to the young group. No age-related difference was detected in serum vitamin C levels. Age correlated positively with serum cholesterol, LDL-cholesterol, PUFA, TBARS, diene conjugates, and negatively with cholesterol-adjusted vitamin E levels and AOA. In addition, endogenous LDL diene conjugate levels and the susceptibility of LDL to copper-induced lipid peroxidation increased in elderly subjects as compared with young subjects. In addition, positive correlations were detected between age and LDL endogenous diene conjugate levels and TBARS formation after copper incubation. However, the susceptibility of whole serum to copper-induced lipid peroxidation did not change in young and elderly subjects. Our results show that endogenous lipid peroxide levels in serum and LDL, and the susceptibility of LDL to copper-induced oxidation, increased with aging in humans.

Vitamins A and E Status in an Urban Lebanese Population: A Case Study at Dar Al-Fatwa Area, Beirut

2006 ◽  
Vol 76 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Omar A. Obeid ◽  
Rola M. Al-Ghali ◽  
Rola M. Al-Ghali ◽  
Nahla Hwalla
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Ldl Cholesterol ◽  
Low Density Lipoprotein ◽  
Plasma Concentrations ◽  
Density Lipoprotein ◽  
Alpha Tocopherol ◽  
Plasma Vitamin ◽  
Low Density

Vitamin A and E status are widely studied in various populations because of their association with several diseases. Fasting plasma vitamin A and E status of 857 Lebanese adults residing in Dar Al-Fatwa, Beirut were assessed using high performance liquid chromatography (HPLC). Mean retinol and alpha-tocopherol concentrations were 59.8 ± 29 µg/dL and 1.0 ± 0.5 mg/dL, respectively in which only 0.2% were retinol-deficient, while 0.7% were alpha-tocopherol-deficient. Vitamin A and E correlated positively with plasma concentrations of total cholesterol, low-density lipoprotein (LDL)-cholesterol, and triglycerides. Vitamin E, but not vitamin A, correlated positively with blood pressure and glucose. A good status of vitamins A and E was found among the studied Lebanese sample and the elevation in vitamin A and E levels was associated with unfavorable lipid profile.

Contribution of vitamin A to the oxidation resistance of human low density lipoproteins

1995 ◽  
Vol 18 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Maria A. Livrea ◽  
Luisa Tesoriere ◽  
Antonino Bongiorno ◽  
Anna Maria Pintaudi ◽  
Marcello Ciaccio ◽  
...  
Keyword(s):  
Vitamin A ◽  
Oxidation Resistance ◽  
Low Density Lipoproteins ◽  
Low Density

Improved method for simultaneous determination of cholesterol in high- and low-density lipoproteins.

1979 ◽  
Vol 25 (8) ◽  
pp. 1480-1481 ◽  
Author(s):  
A Noma ◽  
H Okabe ◽  
K Netsu-Nakayama ◽  
Y Ueno ◽  
H Shinohara
Keyword(s):  
Simultaneous Determination ◽  
Ldl Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Improved Method ◽  
Good Separation ◽  
Assay Precision

Abstract A previously described procedure for simultaneous determination of cholesterol in high- and low-density lipoproteins (HDL and LDL) [Clin. Chem. 24, 1504--1508 (1978)] has been improved by using centrifugation instead of ultrafiltration. Addition of NICl2 (2.0 mmol/L) to the reagent produced a good separation of HDL from the complex of low- and very-low-density lipoprotein-heparin-Ca2+ on centrifugation at 3000 rpm for 15 min. Replicate analyses for high-density lipoprotein cholesterol by the present method demonstrated the following intra-assay precision: mean = 389 mg/L, SD = 11 mg/L, CV = 2.8%. The present (y) and original (x) methods gave results that agreed reasonably well (n = 50, r = 0.960, y = 1.0x + 0.9). The enzymic method for HDL- or (HDL + LDL)-cholesterol after the separation of their fractions gave erroneous results, in particular in the cases of hyperbilirubinemic sera and in (HDL + LDL) fractions.

Changes in plasma lipids after a non-lethal dose of cycloheximide in rats

2003 ◽  
Vol 22 (5) ◽  
pp. 245-248
Author(s):  
A Lucić ◽  
V Bradamante ◽  
M Peraica ◽  
B Radić ◽  
A-M Domijan ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Plasma Lipids ◽  
Lethal Dose ◽  
Hdl Cholesterol ◽  
Low Density Lipoproteins ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Apo B

This paper describes a study of the effect of a single intraperitoneal non-lethal dose of cycloheximide (CHM; 2.0 mg/kg body weight) on the concentration of plasma lipids and lipoproteins in male rats killed one, two, three, four and nine days after receiving the dose. The concentration of triglycerides, total cholesterol, high-density lipoproteins (HDL)-cholesterol and low-density lipoproteins (LDL)-cholesterol was measured in treated and control animals. The effect of CHM on the concentration of triglycerides, total cholesterol, HDL-cholesterol, and LDL-cholesterol was visible in rat plasma throughout the study. Total cholesterol and HDL-cholesterol concentrations showed the same pattern of changes, probably due to the reversible inhibition of apolipoprotein apo A-I synthesis by CHM. The concentration of triglycerides decreased after a lag period of three days when the reserves of apolipoprotein apo B, the main apolipoprotein of very low-density lipoproteins (VLDL)-cholesterols produced in the liver, were consumed.

Sign in / Sign up

Export Citation Format

Share Document