scholarly journals Effects of salbutamol, montelukast and prednisone on orthodontic tooth movement in rats

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Victor Chumpitaz-Cerrate ◽  
Lesly Chávez-Rimache ◽  
César Franco-Quino ◽  
Eliberto Ruiz-Ramirez ◽  
Elías Aguirre-Siancas ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Study Groups ◽  
Preclinical Study ◽  
Control Group ◽  
Histopathological Analysis ◽  
Significant Difference ◽  
Interincisal Distance

Objective: To evaluate the effect of salbutamol, montelukast, and prednisone on orthodontic tooth movement in rats. Material and Methods: In vivo experimental preclinical study. The sample consisted of 48 rats randomly distributed in four study groups. Group A was given saline solution; to group B, salbutamol 4 mg/Kg; to group C, montelukast 2.5 mg/Kg and to group D, prednisone 2.5 mg/Kg. All were fitted with orthodontic devices and the medications were administered intraperitoneally every 12 hours for 5 days. The clinical evaluation (variation in the interincisal distance) was performed at one, three, five, and seven days and the histopathological analysis (cell count) at five and seven days. Results: In the clinical evaluation of the variation in the interincisal distance, a significant difference was found in all the evaluations (p <0.05). It was found that the salbutamol group presented higher variation values in the interincisal distance on all the days evaluated. In the histopathological analysis at five and seven days, it was found that the osteoblast and osteocyte count was significantly higher in the salbutamol group compared to the other groups (p ><0.05). However, in the subgroup analysis, it was found that there was no significant difference in the osteoblast and osteocyte count between the prednisone, montelukast, and control group (p>0.05). Conclusion: The administration of salbutamol increased the magnitude of orthodontic tooth movement; nonetheless, the administration of montelukast and prednisone did not modify the magnitude of orthodontic tooth movement in rats.   Keywords Albuterol; Montelukast; Prednisone; Rats; Tooth movement.

scholarly journals The effect of autologous leukocyte platelet rich fibrin on the rate of orthodontic tooth movement: A prospective randomized clinical trial

2018 ◽  
Vol 12 (03) ◽  
pp. 350-357 ◽  
Author(s):  
Azita Tehranchi ◽  
Hossein Behnia ◽  
Fereydoun Pourdanesh ◽  
Parsa Behnia ◽  
Nelson Pinto ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Tooth Movement ◽  
Random Effect ◽  
Random Effect Model ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Extraction Socket ◽  
Effect Model ◽  
Significant Difference ◽  
And Control

ABSTRACT Objective: The aim of this study was to evaluate the effect of LPRF, placed in extraction sockets, on orthodontic tooth movement (OTM). Materials and Methods: Thirty extraction sockets from eight patients (five males, three females, with a mean age of 17.37 years; range 12–25 years) requiring extraction of first premolars based on their orthodontic treatment plan participated in this split-mouth clinical trial. In one randomly selected quadrant of each jaw, the extraction socket was preserved as the experimental group by immediate placement of LPRF in the extraction socket. The other quadrant served as the control group for secondary healing. Immediately, the teeth adjacent to the defects were pulled together by a NiTi closed-coil spring with constant force. A piece of 0.016 × 0.022-inch stainless steel wire was used as the main arch wire. The amount of OTM was measured on the study casts at eight time points with 2-week intervals for 3 months. Analysis of random effect model was performed for the purpose of comparison between the experimental and control groups. Results: According to the random effect model, a statistically significant difference was found between the experimental and control group in rate of OTM (P = 0.006). Conclusion: According to the results, application of LPRF, as an interdisciplinary approach combining orthodontics and surgery, may accelerate OTM, particularly in extraction cases.

scholarly journals Human Dental Pulp Tissue during Orthodontic Tooth Movement: An Immunofluorescence Study

2020 ◽  
Vol 5 (3) ◽  
pp. 65 ◽  
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Giovanni Matarese ◽  
Angela Militi ◽  
Marco Matarese ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Pulp Tissue ◽  
Force Application ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

The orthodontic tooth movement is the last step of several biological processes that take place after the application of external forces. During this process, dental pulp tissue is subjected to structural and protein expression modifications in order to maintain their integrity and functional morphology. The purpose of the present work was to perform an in vivo study, evaluating protein expression modifications in the human dental pulp of patients that have undergone orthodontic tooth movement due to pre-calibrated light force application for 30 days. Dental pulp samples were extracted from molars and premolars of the control group and after 7 and 30 days of treatment; the samples were then processed for immunofluorescence reactions using antibodies against fibronectin, collagen I and vascular endothelial growth factor (VEGF). Our results show that, after 7 days of treatment, all tested proteins change their pattern expression and will reset after 30 days. These data demonstrate that the dental pulp does not involve any irreversible iatrogenic alterations, supporting the efficacy and safety of using pre-calibrated force application to induce orthodontic tooth movement in clinical practice.

scholarly journals Pain Experience with Micro-osteoperforations during Initial Orthodontic Alignment: A Randomized Clinical Trial.

2020 ◽  
Vol 9 (4) ◽  
pp. 309-318
Author(s):  
Azaitun Akma Shahrin ◽  
◽  
Sarah Haniza Abdul Ghani ◽  
Noraina Hafizan Norman ◽  
◽  
...  
Keyword(s):  
Clinical Trial ◽  
Repeated Measures ◽  
Pain Perception ◽  
Tooth Movement ◽  
Intervention Group ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Initial Alignment ◽  
Significant Difference ◽  
Fixed Appliances

Objetive: The objective of this clinical trial was to investigate the perception of pain during initial maxillary alignment with an adjunctive procedure of micro-osteoperforations (MOPs) compared to conventional orthodontics. Material and methods: This study design was a single-centre, two-arm parallel prospective randomised clinical trial. Thirty consecutive adult subjects (25 females and 5 males; mean age ± SD, 22.66 ± 3.27 years) with 5-8mm moderate upper labial segment crowding were randomly allocated using block randomisation into intervention and control group. All subjects had first premolar extractions, bonded conventional fixed appliances and 0.014-inch nickel-titanium archwire was placed for initial alignment. The intervention group received a 3-mm deep MOPs procedure under local anaesthesia using a Propel device (PROPEL Ortho Singapore) on the labiogingival aspect between the maxillary incisors. Both groups received a set of 100 mm visual analogue scale to complete over the first week, recording pain at 24 hours, 3 days and 1 week. Data were analysed using repeated-measures analysis of variance (ANOVA). Results: There was a statistically significant difference observed in perceived pain levels between MOPs and the control group on day 1, day 3 and day 7 postoperatively. Pain perception was significantly lower in the intervention group at all time points. Conclusion: Accelerating orthodontic tooth movement with MOPs did not accentuate pain perceived during initial maxillary alignment with fixed appliances.

scholarly journals Effects of 405-, 532-, 650-, and 940-nm wavelengths of low-level laser therapies on orthodontic tooth movement in rats

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Hasibe Baser Keklikci ◽  
Ahmet Yagci ◽  
Arzu Hanim Yay ◽  
Ozge Goktepe
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Positive Control ◽  
Control Group ◽  
Bone Area ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
532 Nm

Abstract Background Investigating the effects of 405-nm, 532-nm, 650-nm, and 950-nm wavelengths of LLLTs (low-level laser therapies) on the orthodontic tooth movement in rats by using histological and immunohistochemical methods. Forty-five Wistar albino rats were randomly divided into 5 groups: control group (positive control: the left maxillary 1st molar side; negative control: the right maxillary 1st molar side), 405 nm LLLT group (Realpoo), 532 nm LLLT group (Realpoo), 650 nm LLLT group (Realpoo), and 940 nm LLLT group (Biolase). The left maxillary 1st molar teeth of all rats were applied mesially 50-g force. Starting from the 1st day, 48 h intervals, LLLT was applied in continuous wave mode and in contact with the tissue. The application area was approximately 1 cm2. The lasers were performed for 3 min on each surface (buccal, palatal, mesial), totally 9 min (total dose 54 J/cm2). The amount of the molar mesialization, the bone area between the roots, PDL (periodontal ligament) measurements, TRAP (tartrate-resistant acid phosphatase), and ALP (alkaline phosphatase) immunoreactivity intensity were calculated. Results The amount of the molar mesialization was significantly higher in the 650 nm LLLT group (mean 0.878 ± 0.201 mm; 95% CI (confidence interval) 0.724 and 1.032) than in the groups of positive control (mean 0.467 ± 0.357 mm; 95% CI 0.192 and 0.741) and 405 nm LLLT (mean 0.644 ± 0.261 mm; 95% CI 0.443 and 0.845) (p < 0.001). There were significant differences in the PDL-mesial (p = 0.042) and PDL-distal (p = 0.007) regions between the groups. The immunoreactivity intensity for TRAP-mesial was significantly higher in the positive control group (mean 109,420.33 ± 8769.17; 95% CI 100,217.65 and 118,623.02) than in the 405 nm (mean 91,678.83 ± 7313.39; 95% CI 84,003.9 and 99,353.77) and the 650 nm LLLT (mean 87,169.17 ± 4934.65; 95% CI 81,990.56 and 92,347.77) groups (p = 0.002). There was no statistically significant difference between the groups on immunoreactivity intensity with ALP staining. Conclusions The results of this study show that LLLT with 650-nm wavelength increases orthodontic tooth movement more than 405-nm, 532-nm, and 940-nm LLLTs. The 940-nm and 650-nm LLLTs also increase the bone area between the roots by more than 405-nm and 532-nm wavelengths.

scholarly journals Orthodontic Tooth Movement with Clear Aligners

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Carl T. Drake ◽  
Susan P. McGorray ◽  
Calogero Dolce ◽  
Madhu Nair ◽  
Timothy T. Wheeler
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
University Of Florida ◽  
Material Fatigue ◽  
Significant Difference ◽  
Specific Factors ◽  
The University ◽  
Convenient Model

Clear aligners provide a convenient model to measure orthodontic tooth movement (OTM). We examined the role of in vivo aligner material fatigue and subject-specific factors in tooth movement. Fifteen subjects seeking orthodontic treatment at the University of Florida were enrolled. Results were compared with data previously collected from 37 subjects enrolled in a similar protocol. Subjects were followed prospectively for eight weeks. An upper central incisor was programmed to move 0.5 mm. every two weeks using clear aligners. A duplicate aligner was provided for the second week of each cycle. Weekly polyvinyl siloxane (PVS) impressions were taken, and digital models were fabricated to measure OTM. Initial and final cone beam computed tomography (CBCT) images were obtained to characterize OTM. Results were compared to data from a similar protocol, where subjects received a new aligner biweekly. No significant difference was found in the amount of OTM between the two groups, with mean total OTM of 1.11 mm. (standard deviation (SD) 0.30) and 1.07 mm. (SD 0.33) for the weekly aligner and biweekly control groups, respectively (P=0.72). Over eight weeks, in two-week intervals, material fatigue does not play a significant role in the rate or amount of tooth movement.

Local and systemic effects of low-dose transtympanic methotrexate: in vivo animal study

2012 ◽  
Vol 127 (2) ◽  
pp. 148-152 ◽  
Author(s):  
E Sozen ◽  
S B Erol ◽  
O Yildirim ◽  
B U Coskun ◽  
T Basak ◽  
...  
Keyword(s):  
Animal Study ◽  
Middle Ear ◽  
Low Dose ◽  
Study Groups ◽  
Auditory Brainstem ◽  
Control Group ◽  
Systemic Effects ◽  
Significant Difference ◽  
Brainstem Response

AbstractObjective:To evaluate the safety of low-dose transtympanic methotrexate in a rat model.Design:Experimental animal study.Setting:Tertiary training and research hospital.Methods:Twenty-four rats were randomly divided into three study groups. Diluted methotrexate solution was administered transtympanically to fill the middle-ear cavity, twice a week in group one and three times a week in group two. Ringer lactate solution was administered transtympanically three times a week in the control group.Main outcome measures: Local and systemic effects of low-dose transtympanic methotrexate.Results:In the methotrexate groups, middle-ear mucosal oedema was present in all animals. Auditory brainstem response thresholds indicated no inner-ear dysfunction in any group. Liver function and serum haemoglobin levels showed no statistically significant difference in any group. However, liver biopsies from groups one and two showed mild portal hyperaemia.Conclusion:These findings are encouraging, and support further investigation of the topical application of methotrexate in autoimmune hearing diseases, as an alternative or adjunct to transtympanic steroids.

scholarly journals The effect of red wine extract, resveratrol, on the degree and rate of orthodontic tooth movement in guinea pigs

2015 ◽  
Vol 5 ◽  
pp. 181-189
Author(s):  
Isidro Alex C. Urriquia ◽  
Lotus D. Llavore
Keyword(s):  
Guinea Pigs ◽  
Low Dose ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
High Dose ◽  
Male Adult ◽  
Significant Difference ◽  
The Mean ◽  
And Control

ObjectiveAn animal trial, its protocol approved by the Institutional Animal Care and Use Committee of the U.P. National Institutes of Health (IACUC Protocol No. 2010-008), was employed to investigate the effects of resveratrol on the degree and rate of orthodontic tooth movement in guinea pigs.Materials and MethodsEighteen male adult guinea pigs were randomly allocated into 3 groups: low dose, high dose, and control groups. A 0.016″ titanium molybdenum alloy wire formed into a helical torsion spring with a coil, with the loops cemented onto the maxillary incisors of the animals, served as the orthodontic appliance. Daily oral administration of resveratrol was provided to the low dose (0.047 mg/kg) and high dose (0.47 mg/kg) groups, while water was provided to the control group. Measurements were taken everyday at the interproximal area at the level of the incisal edge using a measuring caliper.ResultsThe results of the ANOVA showed no statistically significant differences in the mean measurements of tooth separation among the three groups from day 2 (P=0.966) to day 8 (P=0.056). However, starting from day 9 (P=0.049) until day 18 (P=0.000), there was a significant difference in the mean tooth separation among the test groups.ConclusionUsing the LSD, it was noted that the low dose and the high dose groups have similar degrees of mean tooth separation, with the control group being significantly different from the two.

Efficacy of Papain-Arginine Gel on Gingivitis Treatment Caused by Orthodontic Appliances

2019 ◽  
Vol 829 ◽  
pp. 203-210
Author(s):  
Jessica Regina ◽  
Angela Putrika Astridtasya ◽  
Maria Rosari Adinda Ningtyas ◽  
Ananto Ali Alhasyimi ◽  
Niswati Fathmah Rosyida
Keyword(s):  
Tooth Movement ◽  
Periodontal Diseases ◽  
Orthodontic Tooth Movement ◽  
Inflammatory Cells ◽  
Untreated Group ◽  
Periodontal Pocket ◽  
Orthodontic Appliances ◽  
Histopathological Analysis ◽  
Significant Difference ◽  
Chlorhexidine Gel

Orthodontic appliances can be linked to a lack of proper oral hygiene which is commonly associated with gingivitis incidence. Gingivitis treatment is required to prevent further periodontal diseases such as periodontitis, pocket formation, tooth luxation, and tooth loss. Papain is known for its anti-inflammatory, antibacterial and antioxidant effects, while arginine is known for its capability to inhibit biofilm formation and interfering plaque stability. This study aimed to demonstrate the effect of papain-arginine gel on gingivitis caused by orthodontic appliances. The gel was prepared from CMC-Na as the polymer. The papain and arginine powder was successfully dissolved homogenously into the CMC-Na in aquadest 50 mL at 25°C. Papain-arginine gel showed good stability in room temperature with pH 7. Therefore, the gel is suitable for oral application. Twenty-seven adult Sprague dawleyrats weighing ± 250–300 g were included in this study. A closing loop was placed in the margin gingiva of animal’s upper incisor to generate orthodontic force and develop reproducible gingivitis. After 7 days of orthodontic tooth movement, animals were randomly divided into three groups; the papain-arginine gel group, the chlorhexidine gel group, and the untreated group. Three rats in every group were conducted clinical observation on its gingival index (GI) and periodontal pocket depths (PPD) before and at 1, 3 and 7 days after treatment. The number of newly-formed capillaries, neutrophils and macrophages proliferation were evaluated using haematoxylin-eosin staining. The clinical response such as increasing GI and decreasing PPD on the treatment did not appear significant difference (P> .05); while the histopathological analysis showed neutrophil and macrophage proliferation were significantly higher andreached its peak on day 3 after papain-arginine and chlorhexidine gel application(P < .05).A considerably larger number of newly-formed capillary formed, and the smaller number of neutrophils and macrophages were noticed significantly at 7 days after papain-arginine and chlorhexidine gel treatment (P> .05). In early gingivitis lesion, gingiva may appear clinically healthy. However, previous studies suggested that inflammatory cells decrease indicates the process of gingivitis healing. Treatment with papain-arginine gel compared to the untreated group significantly diminished the process of inflammation. Hence, it can be concluded that papain-arginine gel was efficient in treating gingivitis caused by orthodontic appliances.

scholarly journals The Effect of Low-Level Laser Therapy on the Acceleration of Orthodontic Tooth Movement

2020 ◽  
Vol 11 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Maryam Baghizadeh Fini ◽  
Pooya Olyaee ◽  
Ahmadreza Homayouni
Keyword(s):  
Clinical Trials ◽  
Laser Therapy ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference

In clinical practice, low-level laser therapy (LLLT) is widely used. The main aim of this review is to assess the effectiveness of LLLT in accelerating tooth movement in human subjects. The PRISMA checklist was utilized as a guideline to carry out this systematic review. The electronic databases were searched from Google Scholar (2014-2018) and PubMed, and comprehensive research on this topic was also manually conducted. Therefore, 77 articles randomized clinical trials (RCTs) or controlled clinical trials (CCTs) were selected. After screening studies, consequently, eleven trials met the inclusion criteria. Eight out of 11 studies showed LLLT has a significant impact on the acceleration of orthodontic tooth movement, and there was no statistically significant difference in the rate of tooth movement between the laser group and the control group in the two remaining studies. Furthermore, five out of 11 articles showed that LLLT has no adverse effects. Although we have some degree of understanding from a cellular point of view to LLLT effects, we still do not know whether these cellular level changes have any effect on the clinical acceleration of orthodontic tooth movement. The results are inconclusive and cannot be generalized to the public community; therefore, well-structured studies are required.

scholarly journals Nicotine effect on bone remodeling during orthodontic tooth movement: Histological study in rats

2014 ◽  
Vol 19 (2) ◽  
pp. 96-107 ◽  
Author(s):  
Ricardo Lima Shintcovsk ◽  
Luégya Knop ◽  
Orlando Motohiro Tanaka ◽  
Hiroshi Maruo
Keyword(s):  
Bone Remodeling ◽  
Tooth Movement ◽  
Histological Study ◽  
Bone Matrix ◽  
Polarized Light ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Orthodontic Movement ◽  
Significant Difference ◽  
Male Wistar Rats

Introduction: Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen. Objective: The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats. Methods: Eighty male Wistar rats were randomly divided into three groups: Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth movement. The animals were euthanized and tissue specimens were processed histologically. We quantified blood vessels, Howship's lacunae and osteoclast-like cells present in the tension and compression areas of periodontal ligaments. The extent of bone formation was evaluated under polarized light to determine the percentage of immature/mature collagen. Results: We observed lower blood vessel densities in the NM group in comparison to the CM group, three (p < 0.001) and seven (p < 0.05) days after force application. Osteoclast-like cells and Howship's lacunae in the NM group presented lower levels of expression in comparison to the CM group, with significant differences on day 7 (p < 0.05 for both variables) and day 14 (p < 0.05 for osteoclast-like cells and p < 0.01 for Howship's lacunae). The percentage of immature collagen increased in the NM group in comparison to the CM group with a statistically significant difference on day 3 (p < 0.05), day 7 (p < 0.001), day 14 (p < 0.001) and day 21 (p < 0.001). Conclusions: Nicotine affects bone remodeling during orthodontic movement, reducing angiogenesis, osteoclast-like cells and Howship's lacunae, thereby delaying the collagen maturation process in developed bone matrix.

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