scholarly journals Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China

2020 ◽  
Vol 6 (4) ◽  
pp. 251
Author(s):  
Asha J. Dissanayake ◽  
Ya-Ya Chen ◽  
Jian-Kui (Jack) Liu
Keyword(s):  
Phylogenetic Analysis ◽  
Elongation Factor ◽  
Nature Reserves ◽  
Guizhou Province ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Molecular Phylogenetic ◽  
Culture Characteristics ◽  
Elongation Factor 1

Though several Diaporthe species have been reported in China, little is known about the species associated with nature reserves in Guizhou province. During a survey of fungi in six nature reserves in Guizhou province of China, thirty-one Diaporthe isolates were collected from different woody hosts. Based on morphology, culture characteristics and molecular phylogenetic analysis, these isolates were characterized and identified. Phylogenetic analysis of internal transcribed spacer region (ITS), combined with translation elongation factor 1-alpha (tef), β-tubulin (tub), calmodulin (cal) and histone H3 (his) gene regions identified five known Diaporthe species and seven distinct lineages representing novel Diaporthe species. The details of five known species: Diaporthe cercidis, D. cinnamomi, D. conica, D. nobilis and D. sackstonii are given and the seven new species D. constrictospora, D. ellipsospora, D. guttulata, D. irregularis, D. lenispora, D. minima, and D. minusculata are introduced with detailed descriptions and illustrations. This study revealed a high diversity of previously undescribed Diaporthe species associated with woody hosts in various nature reserves of Guizhou province, indicating that there is a potential of Diaporthe species remains to be discovered in this unique landform (Karst formations) in China. Interestingly, the five known Diaporthe species have been reported as pathogens of various hosts, and this could indicate that those newly introduced species in this study could be potentially pathogenic pending further studies to confirm.

Amanita griseofusca: A new species of Amanita in section Vaginatae from Malam Jabba, Swat, Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Elongation Factor ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pileus Surface ◽  
Elongation Factor 1 ◽  
A New Species ◽  
Partial Translation

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.

scholarly journals Bambusicolous Arthrinium Species in Guangdong Province, China

2020 ◽  
Vol 11 ◽  
Author(s):  
Indunil C. Senanayake ◽  
Jayarama D. Bhat ◽  
Ratchadawan Cheewangkoon ◽  
Ning Xie
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Guangdong Province ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Bambusicolous Fungi ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

A survey of bambusicolous fungi in Bijiashan Mountain Park, Shenzhen, Guangdong Province, China, revealed several Arthrinium-like taxa from dead sheaths, twigs, and clumps of Bambusa species. Phylogenetic relationships were investigated based on morphology and combined analyses of the internal transcribed spacer region (ITS), large subunit nuclear ribosomal DNA (LSU), beta tubulin (β-tubulin), and translation elongation factor 1-alpha (tef 1-α) gene sequences. Based on morphological characteristics and phylogenetic data, Arthrinium acutiapicum sp. nov. and Arthrinium pseudorasikravindrae sp. nov. are introduced herein with descriptions and illustrations. Additionally, two new locality records of Arthrinium bambusae and Arthrinium guizhouense are described and illustrated.

Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 28 ◽  
Author(s):  
KAI CHEN ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Culture Characteristics ◽  
Close Relatives ◽  
Encoding Genes ◽  
Elongation Factor 1

Seven new species of the genus Trichoderma belonging to the Viride clade are described from soil samples of different regions in China, and named as T. adaptatum, T. beijingense, T. bifurcatum, T. mangshanicum, T. shaoguanicum, T. tardum and T. vulgatum. Their phylogenetic positions are determined by analyses of the combined partial sequences of translation elongation factor 1-alpha and the second largest RNA polymerase subunit encoding genes. Morphology and culture characteristics are observed, described and illustrated in detail. All the new species form green conidia, fit nicely the Viride clade, but differ obviously from any known species of the genus in sequence data, colony features, growth rates, and size of phialides and conidia. Distinctions between the new species and their close relatives are discussed.

scholarly journals Neopestalotiopsis Species Associated with Flower Diseases of Macadamia integrifolia in Australia

2021 ◽  
Vol 7 (9) ◽  
pp. 771
Author(s):  
Kandeeparoopan Prasannath ◽  
Roger G. Shivas ◽  
Victor J. Galea ◽  
Olufemi A. Akinsanmi
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Macadamia Integrifolia ◽  
Eastern Australia ◽  
Elongation Factor 1 Alpha ◽  
Molecular Phylogenetic ◽  
Flower Disease ◽  
Elongation Factor 1

Macadamia (Macadamia integrifolia) is native to eastern Australia and produces an edible nut that is extensively cultivated in commercial orchards in several countries. Little is known about the diversity of fungi associated with diseases of macadamia inflorescences. A survey of fungi associated with the dry flower disease of macadamia detected several isolates of Neopestalotiopsis (Pestalotiopsidaceae, Sordariomycetes). Five new species of Neopestalotiopsis were identified based on molecular phylogenetic analyses of concatenated gene sequences of the internal transcribed spacer (ITS), β-tubulin (TUB), and the translation elongation factor 1-alpha (TEF1α). The new species are named Neopestalotiopsis drenthii, N. maddoxii, N. olumideae, N. vheenae, and N. zakeelii, and are described by molecular, morphological, and cultural characteristics. The ecology of the isolates and their pathogenic, saprophytic, or commensal ability were not determined.

A new species of Scleroramularia associated with sooty blotch and flyspeck in Southern China

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 261 ◽  
Author(s):  
Liu Gao ◽  
Zhuoya Wei ◽  
Bo Wang ◽  
Mark L. Gleason ◽  
Rong Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Elongation Factor ◽  
Space Region ◽  
Translation Elongation ◽  
Sooty Blotch ◽  
Elongation Factor 1 Alpha ◽  
Sooty Blotch And Flyspeck ◽  
Elongation Factor 1 ◽  
A New Species

Scleroramularia is a genus that includes species of the sooty blotch and flyspeck (SBFS) fungal complex that blemishes the surface of apple, pawpaw, and other fruit. In a survey of SBFS on banana (Musa basjoo), an isolate associated with the flyspeck mycelial type of SBFS was obtained from Zhanjiang, Guangdong, China. Based on analysis of morphology and phylogeny (the nuclear ribosomal internal transcribed space region and translation elongation factor 1-alpha), it was delimited as a new species of Scleroramularia, described here as S. musae. Conidia of S. musae have more septa than other species presently known in the genus.

Saprobic Dothideomycetes in Thailand: Phaeoseptum hydei sp. nov., a new terrestrial ascomycete in Phaeoseptaceae

Phytotaxa ◽  
2020 ◽  
Vol 449 (2) ◽  
pp. 149-163
Author(s):  
DHANUSHKA N. WANASINGHE ◽  
PETER E. MORTIMER ◽  
CHANOKNED SENWANNA ◽  
RATCHADAWAN CHEEWANGKOON
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
University Campus ◽  
Translation Elongation ◽  
Delonix Regia ◽  
The Family ◽  
Elongation Factor 1 Alpha ◽  
Vertical Septum ◽  
Elongation Factor 1

During a survey of saprobic microfungi in Thailand, a dothideomycetous fungus was found on a dead twig of Delonix regia, on the Chiang Mai University campus. This fungus is characterized by fully immersed ascomata under a small blackened pseudoclypeus, pseudoparenchymatous peridium, cellular pseudoparaphyses, cylindrical-clavate asci with a distinct pedicel, overlapping 3–4-seriate, pale to dark brown, broadly fusoid, 7–9-transversally septate ascospores with a vertical septum in nearly all median cells. Multigene phylogenetic analyses, using partial sequences from the 28S nrRNA gene (LSU), 18S nrRNA gene (SSU), internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon and the translation elongation factor 1-alpha region (TEF) demonstrated a monophyletic affiliation of the new strain, accommodating the species of Phaeoseptum in the family Phaeoseptaceae. With further morphological and phylogenetic investigations, we justify the new fungus as a novel species, Phaeoseptum hydei in Phaeoseptaceae. Detailed descriptions and illustrations are provided for Phaeoseptum hydei and this novel species compared with the remaining species found in the genus. An updated checklist of microfungi recorded on Delonix regia from around the world is also provided.

scholarly journals Molecular characterization and phylogenetic analyses of Lophodermella needle pathogens (Rhytismataceae) on Pinus species in the USA and Europe

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11435
Author(s):  
Jessa P. Ata ◽  
Kelly S. Burns ◽  
Suzanne Marchetti ◽  
Isabel A. Munck ◽  
Ludwig Beenken ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Phenotypic Characters ◽  
Elongation Factor 1 Alpha ◽  
The Usa ◽  
Phylogenetic Resolution ◽  
Elongation Factor 1

Increasing prevalence of conifer needle pathogens globally have prompted further studies on pathogen identification and a better understanding of phylogenetic relationships among needle pathogens. Several Lophodermella species can be aggressive pathogens causing needle cast in natural pine forests in the USA and Europe. However, their relationships with other Rhytismataceae species have historically been based on similarities of only limited phenotypic characters. Currently, no molecular studies have been completed to elucidate their relationships with other Lophodermella needle pathogens. This study collected and sequenced three gene loci, namely: internal transcribed spacer, large ribosomal subunit, and translation elongation factor 1-alpha, from five Lophodermella needle pathogens from North America (L. arcuata, L. concolor, L. montivaga) and Europe (L. conjuncta and L. sulcigena) to distinguish phylogeny within Rhytismatacaeae, including Lophophacidium dooksii. Phylogenetic analyses of the three loci revealed that all but L. conjuncta that were sampled in this study consistently clustered in a well-supported clade within Rhytismataceae. The multi-gene phylogeny also confirmed consistent nesting of L. dooksii, a needle pathogen of Pinus strobus, within the clade. Potential synapomorphic characters such as ascomata position and ascospore shape for the distinct clade were also explored. Further, a rhytismataceous species on P. flexilis that was morphologically identified as L. arcuata was found to be unique based on the sequences at the three loci. This study suggests a potential wider range of host species within the genus and the need for genetic characterization of other Lophodermella and Lophophacidium species to provide a higher phylogenetic resolution.

scholarly journals Ramichloridium endophyticum sp. nov., a novel species of endophytic fungus from Potamogeton pectinatus

2020 ◽  
Vol 70 (5) ◽  
pp. 3433-3439
Author(s):  
Hua Zheng ◽  
Zefen Yu ◽  
Jianping Xu ◽  
Rafael F. Castañeda-Ruiz ◽  
Min Qiao
Keyword(s):  
Novel Species ◽  
Elongation Factor ◽  
Internal Transcribed Spacers ◽  
Potamogeton Pectinatus ◽  
Full Description ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pr China ◽  
Alpha Gene ◽  
Elongation Factor 1

During a survey of endophytic fungi in aquatic plants collected from Tibet, PR China, a novel species, Ramichloridium endophyticum, was isolated from Potamogeton pectinatus. This novel species differs from other species of the genus Ramichloridium by its finely verrucose, obovoid, ellipsoidal–obovoid and occasionally subglobose conidia. Phylogenetic analysis of the combined sequences of the internal transcribed spacers (ITS) and the translation elongation factor 1-alpha gene (tef1-α) confirmed that the isolated strain represents a member of the genus Ramichloridium. A full description, illustrations and a phylogenetic tree showing the position of R. endophyticum are provided.

scholarly journals Four new East Asian species of Aleurodiscus with echinulate basidiospores

MycoKeys ◽  
2019 ◽  
Vol 52 ◽  
pp. 71-87
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Yu-Ting Lin ◽  
Chiung-Chih Chang ◽  
Shuang-Hui He
Keyword(s):  
New Species ◽  
Southwest China ◽  
East Asian ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Northwest Yunnan ◽  
Elongation Factor 1 Alpha ◽  
Alpha Gene ◽  
Elongation Factor 1

Four new species of Aleurodiscus sensu lato with echinulate basidiospores are described from East Asia: A.alpinus, A.pinicola, A.senticosus, and A.sichuanensis. Aleurodiscusalpinus is from northwest Yunnan of China where it occurs on Rhododendron in montane habitats. Aleurodiscuspinicola occurs on Pinus in montane settings in Taiwan and northwest Yunnan. Aleurodiscussenticosus is from subtropical Taiwan, where it occurs on angiosperms. Aleurodiscussichuanensis is reported from southwest China on angiosperms in montane environments. Phylogenetic relationships of these four new species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1.

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