scholarly journals Physicochemical and Biological Characterization of Ti6Al4V Particles Obtained by Implantoplasty: An In Vitro Study. Part I

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6507
Author(s):  
Jorge Toledano-Serrabona ◽  
Francisco Javier Gil ◽  
Octavi Camps-Font ◽  
Eduard Valmaseda-Castellón ◽  
Cosme Gay-Escoda ◽  
...  
Keyword(s):  
Cell Viability ◽  
Specific Surface ◽  
Surface Area ◽  
Specific Surface Area ◽  
In Vitro Study ◽  
Vitro Study ◽  
Equivalent Diameter ◽  
Metal Debris ◽  
Aluminum Ions

Implantoplasty is a mechanical decontamination technique that consists of polishing the supra-osseous component of the dental implant with peri-implantitis. This technique releases metal particles in the form of metal swarf and dust into the peri-implant environment. In the present in vitro study, the following physicochemical characterization tests were carried out: specific surface area, granulometry, contact angle, crystalline structure, morphology, and ion release. Besides, cytotoxicity was in turn evaluated by determining the fibroblastic and osteoblastic cell viability. As a result, the metal debris obtained by implantoplasty presented an equivalent diameter value of 159 µm (range 6–1850 µm) and a specific surface area of 0.3 m2/g on average. The particle had a plate-like shape of different sizes. The release of vanadium ions in Hank’s solution at 37 °C showed no signs of stabilization and was greater than that of titanium and aluminum ions, which means that the alloy suffers from a degradation. The particles exhibited cytotoxic effects upon human osteoblastic and fibroblastic cells in the whole extract. In conclusion, metal debris released by implantoplasty showed different sizes, surface structures and shapes. Vanadium ion levels were higher than that those of the other metal ions, and cell viability assays showed that these particles produce a significant loss of cytocompatibility on osteoblasts and fibroblasts, which means that the main cells of the peri-implant tissues might be injured.

scholarly journals Cytotoxicity induced by new spiral mesoporous silica nanorods via specific surface area and ROS accumulation in HeLa cells

2020 ◽  
Vol 1 (9) ◽  
pp. 3556-3564
Author(s):  
Lan She ◽  
Miao Sun ◽  
Xinfang Li ◽  
Anfeng Kang ◽  
Feng Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mesoporous Silica ◽  
Cell Viability ◽  
Specific Surface ◽  
Surface Area ◽  
Specific Surface Area ◽  
Hela Cells ◽  
Ros Accumulation ◽  
Silica Nanorods

Results indicated that the effect of MSNRs on cell viability and cellular oxidative stress was related to specific surface area.

Surface Analysis of Etched Molar Enamel by Gas Adsorption

2008 ◽  
Vol 87 (6) ◽  
pp. 532-536 ◽  
Author(s):  
M.F. Orellana ◽  
A.E. Nelson ◽  
J.P.R. Carey ◽  
G. Heo ◽  
D.G Boychuk ◽  
...  
Keyword(s):  
Specific Surface ◽  
Surface Area ◽  
Specific Surface Area ◽  
Gas Adsorption ◽  
Third Molar ◽  
Dental Enamel ◽  
Total Surface Area ◽  
Gas Absorption ◽  
Etch Pattern

Much research has been devoted to the study of etched enamel, since it is critical to bonding. Currently, there are no precise data regarding the etched-enamel specific surface area. The aim of this study was to characterize, by two different methods, the surface of human dental enamel in vitro after being etched. It was hypothesized that differences would be observed between specimens in terms of specific surface area and grade of etching. Sixteen third molar enamel samples were etched for 30 sec with 37% phosphoric acid prior to being viewed by SEM. Etched enamel surfaces were graded according to the Galil and Wright classification. The total surface area of etched samples was determined by the BET gas absorption method. A substantial variability in total surface area was observed between and among samples. A Pearson’s Correlation Coefficient showed a lack of relationship between etch pattern and total surface area.

scholarly journals The impact of electronic cigarette liquids on human gingival cell viability – a preliminary in vitro study

Dental Forum ◽  
2016 ◽  
Vol 44 (1) ◽  
pp. 17-20
Author(s):  
Iwona Inkielewicz‑Stępniak ◽  
Aida Kusiak ◽  
Anna Wojtaszek‑Słomińska ◽  
Karolina Niska ◽  
Barbara Szkarłat
Keyword(s):  
Cell Viability ◽  
In Vitro Study ◽  
Electronic Cigarette ◽  
Vitro Study ◽  
The Impact

scholarly journals Nyquist limit and orifice area independently affect the accuracy of proximal isovelocity surface area estimation of flow rate:An in vitro study

1990 ◽  
Vol 15 (2) ◽  
pp. A109 ◽  
Author(s):  
Leonardo Rodriquez ◽  
Joseph Anconina ◽  
Pamela Harrigan ◽  
Robert A Levine ◽  
Victor H Monterroso ◽  
...  
Keyword(s):  
Surface Area ◽  
In Vitro Study ◽  
Vitro Study ◽  
Orifice Area ◽  
Area Estimation ◽  
Proximal Isovelocity Surface Area ◽  
Nyquist Limit

Coconut milk and probiotic milk as storage media to maintain periodontal ligament cell viability: an in vitro study

2016 ◽  
Vol 33 (3) ◽  
pp. 160-164 ◽  
Author(s):  
Divya Saini ◽  
Prahlad Gadicherla ◽  
Prakash Chandra ◽  
Latha Anandakrishna
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Coconut Milk ◽  
Vitro Study ◽  
Periodontal Ligament Cell ◽  
Storage Media

Non-Clinical investigation of Tuberculosis Drugs - Conjugated Norbornene-Based Nanocarriers Toxic Impacts on Zebrafish

2021 ◽  
Vol 12 ◽  
Author(s):  
Thangammal Anju ◽  
Radhakrishnan Preetha ◽  
Raja Shunmugam ◽  
Shivshankar R Mane ◽  
Jesu Arockiaraj ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Viability ◽  
Hepg2 Cells ◽  
In Vitro Study ◽  
Liver Cells ◽  
Vitro Study ◽  
Ros Generation ◽  
Human Liver Cell ◽  
Zebrafish Liver

INTRODUCTION: Rifampicin conjugated (R-CP), and rifampicin -isoniazid dual conjugated (RI-CP) norbornene-derived nanocarriers are newly designed for pH stimuli-responsive delivery of tuberculosis (TB) drugs. Its biosafety level is yet to be well established. OBJECTIVES: To assess the impacts of the nanocarriers on liver cells using zebrafish animal model and human liver cell line model (HepG2). METHODS: Initially, lethal dose concentration for the norbornene-derived nanocarrier systems in zebrafish was determined. The toxic effects were analysed at the sub-lethal drug concentration by histopathological study, total GSH level, gene expression and DNA damage in zebrafish liver cells. Fish erythrocyte nuclear abnormalities were also evaluated. Cell viability and oxidative stress level (ROS generation) after exposure to the nanoconjugates was determined using HepG2 cell in the in vitro study. RESULTS: In vivo studies of both R-CP and RI-CP showed 100% mortality at 96 hours for exposure concentration >100mg/l and showed toxic changes in zebrafish liver histology, GSH, and DNA damage levels. A noticeable upregulated PXR, CYP3A and cyp2p6 genes was observed in RI-CP exposure than in RIF or R-CP molecules. The in vitro study revealed a dose-dependent effect on cell viability and ROS generation for RIF, R-CP and RI-CP exposures in HepG2 cells. CONCLUSION: The current study reports that the rifampicin conjugated (R-CP) and rifampicin-isoniazid conjugated (RI-CP) norbornene derived nanocarriers exhibit enhanced toxic responses in both adult zebrafish and HepG2 cells. The pH-sensitive norbornene derived nanocarriers on conjugation with different drugs exhibited varied impacts on hepatic cells. Hence the present investigation recommends a complete metabolomics analysis and norbornene carrier-drug interaction study to be performed for each drug conjugated norbornene nanocarrier to ensure its biosafety.

scholarly journals Mesoporous Iron(III)-Doped Hydroxyapatite Nanopowders Obtained via Iron Oxalate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 811
Author(s):  
Margarita A. Goldberg ◽  
Marat R. Gafurov ◽  
Fadis F. Murzakhanov ◽  
Alexander S. Fomin ◽  
Olga S. Antonova ◽  
...  
Keyword(s):  
Specific Surface ◽  
Surface Area ◽  
Specific Surface Area ◽  
Density Functional ◽  
Covalent Bond ◽  
In Vitro Tests ◽  
Crystalline Region ◽  
Fe Oxides ◽  
Iron Oxalate

Mesoporous hydroxyapatite (HA) and iron(III)-doped HA (Fe-HA) are attractive materials for biomedical, catalytic, and environmental applications. In the present study, the nanopowders of HA and Fe-HA with a specific surface area up to 194.5 m2/g were synthesized by a simple precipitation route using iron oxalate as a source of Fe3+ cations. The influence of Fe3+ amount on the phase composition, powders morphology, Brunauer–Emmett–Teller (BET) specific surface area (S), and pore size distribution were investigated, as well as electron paramagnetic resonance and Mössbauer spectroscopy analysis were performed. According to obtained data, the Fe3+ ions were incorporated in the HA lattice, and also amorphous Fe oxides were formed contributed to the gradual increase in the S and pore volume of the powders. The Density Functional Theory calculations supported these findings and revealed Fe3+ inclusion in the crystalline region with the hybridization among Fe-3d and O-2p orbitals and a partly covalent bond formation, whilst the inclusion of Fe oxides assumed crystallinity damage and rather occurred in amorphous regions of HA nanomaterial. In vitro tests based on the MG-63 cell line demonstrated that the introduction of Fe3+ does not cause cytotoxicity and led to the enhanced cytocompatibility of HA.

Sign in / Sign up

Export Citation Format

Share Document