scholarly journals The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles

2021 ◽  
Vol 7 (9) ◽  
pp. 128
Author(s):  
Ekaterina Bobrikova ◽  
Alexey Chubarov ◽  
Elena Dmitrienko
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Stable Complex ◽  
Oxide Nanoparticles ◽  
Nucleic Acid Delivery ◽  
Transmission Electron ◽  
Species Formation ◽  
High Reactive Oxygen Species ◽  
Ph Buffer ◽  
Lung Adenocarcinoma A549

Magnetic Fe3O4 nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe3O4 MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.

Preparation and Characterization of Ultrafine Monodisperse Iron Oxide Nanoparticles by Solvothermal Method

2015 ◽  
Vol 748 ◽  
pp. 93-96
Author(s):  
Cheng Mei Liu ◽  
Yu Xia Zhao ◽  
Jin Dong ◽  
Lu Hai Li ◽  
Yen Wei ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Solvothermal Method ◽  
Physical Property Measurement System ◽  
Thermo Gravimetric Analysis ◽  
Physical Property ◽  
Oxide Nanoparticles ◽  
Gravimetric Analysis ◽  
Transmission Electron ◽  
Different Diameters

Using iron-oleate complex as a precursor, oleic acid as a stabilizer and 1-octadecene as a reductant, uniform-sized and highly monodisperse iron oxide nanoparitcles with different diameters were successfully synthesized via solvothermal method by changing reaction time. Transmission electron microscope (TEM), thermo-gravimetric analysis (TGA), fourier transform infrared spectroscopy (FT-IR), physical property measurement system (PPMS) and dynamic light scattering (DLS) was used to characterize obtained iron oxide nanoparticles. These results indicated that iron oxide nanoparitcles with the diameter ranging from 4 to 8 nm can be controllably synthesized.

Iron Oxide Nanoparticles Obtained from a Fe(II) - Chitosan Polymer Film

2010 ◽  
Vol 644 ◽  
pp. 51-55 ◽  
Author(s):  
Juan Fco Luna Martínez ◽  
E. Reyes-Melo ◽  
Virgilio González-González ◽  
A. Torres-Castro ◽  
Carlos Guerrero-Salazar ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Composite Film ◽  
Polymer Film ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Behavior ◽  
Nanostructured Material ◽  
Oxide Nanoparticles ◽  
Transmission Electron ◽  
Alkaline Conditions

In this work, iron oxide nanoparticles (~5 nm) embedded in a chitosan polymer film, were synthesized. In order to obtain this nanostructured material, firstly a homogenous film of Fe(II)-chitosan was prepared. The resulting composite film has a thickness of ~140μm. Iron oxide nanoparticles were in-situ synthesized by treating the composite film with H2O2 under alkaline conditions. The morphological analysis by Transmission Electron Microscopy (TEM) shows the nanoparticles were embedded and stabilized in chitosan polymer film. The magnetic behavior was studied by magnetization measurements. The magnetization curves at room temperature showed that iron oxide nanoparticles have a superparamagnetic behavior.

scholarly journals Synthesis and characterization of dextran-coated iron oxide nanoparticles

2018 ◽  
Vol 5 (3) ◽  
pp. 171525 ◽  
Author(s):  
Andra Mihaela Predescu ◽  
Ecaterina Matei ◽  
Andrei Constantin Berbecaru ◽  
Cristian Pantilimon ◽  
Claudia Drăgan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Quantum Interference ◽  
Synthesis And Characterization ◽  
Oxide Nanoparticles ◽  
Transmission Electron ◽  
Molar Weight ◽  
Co Precipitation

Synthesis and characterization of iron oxide nanoparticles coated with a large molar weight dextran for environmental applications are reported. The first experiments involved the synthesis of iron oxide nanoparticles which were coated with dextran at different concentrations. The synthesis was performed by a co-precipitation technique, while the coating of iron oxide nanoparticles was carried out in solution. The obtained nanoparticles were characterized by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction spectrometry, Fourier transform infrared spectroscopy and superconducting quantum interference device magnetometry. The results demonstrated a successful coating of iron oxide nanoparticles with large molar weight dextran, of which agglomeration tendency depended on the amount of dextran in the coating solution. SEM and TEM observations have shown that the iron oxide nanoparticles are of about 7 nm in size.

scholarly journals Synthesis of Oxide Iron Nanoparticles Using Laser Ablation for Possible Hyperthermia Applications

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2099
Author(s):  
María J. Rivera-Chaverra ◽  
Elisabeth Restrepo-Parra ◽  
Carlos D. Acosta-Medina ◽  
Alexandre. Mello ◽  
Rogelio. Ospina
Keyword(s):  
Iron Oxide ◽  
Laser Ablation ◽  
Iron Oxide Nanoparticles ◽  
Laser Energy ◽  
Great Influence ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Heating Capacity ◽  
Nanoparticles Concentration

In this work, iron oxide nanoparticles produced using the laser ablation technique were studied in order to determine the characteristics of these nanoparticles as a function of the laser energy for the possible application in magnetic hyperthermia. Nanoparticles were obtained by varying the power of the laser considering values of 90, 173, 279 and 370 mJ. The morphology of these nanoparticles was determined using the dynamic light scattering (DLS) and scattering transmission electron microscopy (STEM) techniques, confirming that the size of the particles was in the order of nanometers. A great influence of the laser power on the particle size was also observed, caused by the competition between the energy and the temperature. The composition was determined by X-ray diffraction and Raman spectroscopy, showing the presence of magnetite, maghemite and hematite. The hyperthermia measurements showed that the temperature rise of the iron oxide nanoparticles was not greatly influenced by the energy change, the heating capacity of magnetic NPs is quantified by the specific absorption rate (SAR), that tends to decrease with increasing energy, which indicates a dependence of these values on the nanoparticles concentration.

scholarly journals One step paired electrochemical synthesis of iron and iron oxide nanoparticles

2016 ◽  
Vol 34 (3) ◽  
pp. 655-658 ◽  
Author(s):  
Juliet Ordoukhanian ◽  
Hassan Karami ◽  
Azizollah Nezhadali
Keyword(s):  
Electron Microscopy ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Low Cost ◽  
Electrolytic Cell ◽  
Oxide Nanoparticles ◽  
Transmission Electron ◽  
One Step ◽  
Application Potential ◽  
Reduction Of Energy Consumption

AbstractIn this study, a new one step paired electrochemical method is developed for simultaneous synthesis of iron and iron oxide nanoparticles. iron and iron oxide are prepared as cathodic and anodic products from iron (ii) sulfate aqueous solution in a membrane divided electrolytic cell by the pulsed current electrosynthesis. Because of organic solvent-free and electrochemical nature of the synthesis, the process could be considered as green and environmentally friendly. The reduction of energy consumption and low cost are the other significant advantages of this new method that would have a great application potential in the chemical industry. The nanostructure of prepared samples was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The magnetic properties were studied by vibrating sample magnetometer (VsM).

Iron Salt Concentration Effect to the Precipitation of Iron Oxide Nanoparticles Conjugated Antibody

2012 ◽  
Vol 620 ◽  
pp. 268-272
Author(s):  
Mohamad Nor Noorhashimah ◽  
Abdul Razak Khairunisak ◽  
Azlan Abdul Aziz ◽  
Rahmah Noordin
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Optimum Concentration ◽  
Precipitation Method ◽  
Oxide Nanoparticles ◽  
Ferrous Chloride ◽  
Iron Salt ◽  
Transmission Electron ◽  
Polyethelene Glycol ◽  
Conjugation Process

This study covers the effect of ferrous chloride (FeCl2) concentration on the formation of iron oxide nanoparticles (IONPs) via the precipitation method. IONPs with appropriate surface functionalization were synthesized in order to obtain a stable colloidal IONPs (ferrofluid) for the conjugation process. The electrostatic repulsion using percloric acid (HClO4) and steric stabilization using silane polyethelene glycol (SiPEG) were generated. The optimum concentration of FeCl2was at 0.3 M. From the transmission electron microscopy (TEM) image, the size of IONPs obtained was ~13 nm. The stable IONPs were then conjugated to the antibody and tested in the lateral flow immunoassay as the labelling agent. The conjugated IONPS with the antibody was proven sensitive to the Brugian Filiarisis disease.

scholarly journals Phytosynthesis of Iron Oxide Nanoparticles using Juncus inflexus Shoot Extract

2021 ◽  
Vol 12 (3) ◽  
pp. 3790-3799
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Low Cost ◽  
Blue Dye ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Polymorphic Composition

To elude the toxic effects of chemically synthesized nanoparticles, the phytochemically synthesized nanoparticles may provide a better alternative. For the first time, an aqueous extract of Juncus inflexus shoot with FeCl3.6H2O was used for the phytosynthesis of iron oxide nanoparticles (FeONPs). As-synthesized FeONPs were characterized by UV-Vis spectroscopy, Transmission electron microscopy (TEM), Dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). FeONPs showed UV-vis absorption spectra between 300-400 nm, whereas TEM analysis confirmed the particle sizes of 40-60 nm with aggregation. XRD is confirming the polymorphic composition of Fe3O4, α-Fe2O3, and Fe0 nanoparticles. Furthermore, FTIR analysis presenting the most probable mechanism for the synthesis of FeONPs. This multiphase FeONPs was applied for the decolorization of methylene blue dye (>83%). Phytosynthesized FeONPs have the benefits of low cost, no toxicity, sustainable, and eco-friendly technology so that they may be used as adsorbent/catalyst for remediation of toxic dyes in an aqueous medium.

Design and development of peptide-functionalized iron oxide nanoparticles: Investigation of ultrasound imaging for the diagnosis and treatment of cancers

2021 ◽  
Vol 11 (6) ◽  
pp. 817-823
Author(s):  
Meijun Mao ◽  
Minqing Zhang ◽  
Wei Xu ◽  
Bei Zhao
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Ultrasound Imaging ◽  
Particle Surface ◽  
Oxide Nanoparticles ◽  
Average Particle ◽  
Hek 293 ◽  
Transmission Electron ◽  
Contrast Analysis

Iron oxide nanoparticles (Fe3O4 NPs) with superparamagnetic characteristics were synthesized and coated with glypican-3 ligand peptide (GPC3), resulting in GPC3@Fe3O4 NPs. Fourier-transform infrared spectroscopy and high resolution transmission electron microscopy (HRTEM) were used to characterize the fabricated GPC3@Fe3O4 NPs, and dynamic light scattering measurements were performed to determine the average particle surface charge. The morphology and size distributions of the uncoated Fe3O4 NPs were determined using HRTEM images, which revealed uniform-sized GPC3@Fe3O4 NPs with a particle size of approximately 10 nm. An in vitro cell proliferation assay was performed on non-cancerous HEK-293 cells to examine the cytotoxicity of free Fe3O4 NPs, free GPC3, and GPC3@Fe3O4 NPs. The potential of the engineered GPC3@Fe3O4 NPs to act as contrast agents for enhanced ultrasound imaging was also examined in vitro. The results showed that GPC3@Fe3O4 NPs significantly improved the effective ultrasound contrast analysis for cancer detection and treatment.

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