Characterization of a New Trioxilin and a Sulfoquinovosyl Diacylglycerol with Anti-Inflammatory Properties from the Dinoflagellate Oxyrrhis marina

(1) Background: Both sensory quality and healthy attributes of Vitis vinifera grapes used for winemaking are closely related with the polyphenolic composition of their skins. (2) Methods: In this study, the polyphenolic characterization (flavan-3-ols, procyanidins, flavonols, stilbenes, anthocyanins) was investigated by ultra performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UPLC-QqQ-MS). Skins from Vitis vinifera Merlot, Tannat, and Syrah red grape varieties cultivated in the south of France at different stages of ripening in 2018 were used. The anti-inflammatory and the antioxidant potential of the extracts were evaluated by the measure of nitric oxide (NO) and the intracellular reactive oxygen species production (ROS) in lipopolysaccharide (LPS)-stimulated macrophages. (3) Results: 41 polyphenols were quantified in all samples. Generally, the flavan-3-ol and procyanidin content decreased during ripening whereas the anthocyanins and stilbenes increased. In addition, as a novelty of this work, a wide identification and characterization of monomeric and oligomeric stilbenes was assessed by using authentic standards isolated in our laboratory, some of them (parthenocissin A and miyabenol C) reported for the first time in Merlot, Tannat and Syrah cultivars. The before-veraison skin extracts of all studied varieties, exhibited higher NO and ROS productions inhibition (>50%) proving both antioxidant and anti-inflammatory properties.

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Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization

Scientific Reports ◽

10.1038/s41598-021-94181-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Masuzu Kikuchi ◽

Keiichi Kojima ◽

Shin Nakao ◽

Susumu Yoshizawa ◽

Shiho Kawanishi ◽

...

Keyword(s):

Escherichia Coli ◽

Proton Pump ◽

Expression System ◽

Spectroscopic Characterization ◽

Functional Expression ◽

Proton Pumping ◽

E Coli ◽

Oxyrrhis Marina ◽

Domains Of Life

AbstractMicrobial rhodopsins are photoswitchable seven-transmembrane proteins that are widely distributed in three domains of life, archaea, bacteria and eukarya. Rhodopsins allow the transport of protons outwardly across the membrane and are indispensable for light-energy conversion in microorganisms. Archaeal and bacterial proton pump rhodopsins have been characterized using an Escherichia coli expression system because that enables the rapid production of large amounts of recombinant proteins, whereas no success has been reported for eukaryotic rhodopsins. Here, we report a phylogenetically distinct eukaryotic rhodopsin from the dinoflagellate Oxyrrhis marina (O. marina rhodopsin-2, OmR2) that can be expressed in E. coli cells. E. coli cells harboring the OmR2 gene showed an outward proton-pumping activity, indicating its functional expression. Spectroscopic characterization of the purified OmR2 protein revealed several features as follows: (1) an absorption maximum at 533 nm with all-trans retinal chromophore, (2) the possession of the deprotonated counterion (pKa = 3.0) of the protonated Schiff base and (3) a rapid photocycle through several distinct photointermediates. Those features are similar to those of known eukaryotic proton pump rhodopsins. Our successful characterization of OmR2 expressed in E. coli cells could build a basis for understanding and utilizing eukaryotic rhodopsins.

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Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2010.09.010 ◽

2011 ◽

Vol 48 (1) ◽

pp. 38-43 ◽

Cited By ~ 52

Author(s):

Swagata Das ◽

Biswajit Mishra ◽

Kamaldeep Gill ◽

Md. Saquib Ashraf ◽

Abhay Kumar Singh ◽

...

Keyword(s):

Aloe Vera ◽

Isolation And Characterization ◽

Anti Inflammatory ◽

Anti Fungal ◽

Novel Protein

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Synthesis and Characterization of Metabolites and Potential Impurities of Balsalazide Disodium, an Anti-Inflammatory Drug

Synthetic Communications ◽

10.1080/00397910903221068 ◽

2010 ◽

Vol 40 (15) ◽

pp. 2241-2253 ◽

Cited By ~ 8

Author(s):

Md. Umar Khan ◽

M. A. Baseer ◽

S. Ranjith Kumar ◽

M. Saravanakumar ◽

A. G. Prasannanjali ◽

...

Keyword(s):

Synthesis And Characterization ◽

Inflammatory Drug ◽

Anti Inflammatory

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Characterization of bergenin in Endopleura uchi bark and its anti-inflammatory activity

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532009000600009 ◽

2009 ◽

Vol 20 (6) ◽

pp. 1060-1064 ◽

Cited By ~ 39

Author(s):

Rita C. S. Nunomura ◽

Viviane G. Oliveira ◽

Saulo L. Da Silva ◽

Sergio M. Nunomura

Keyword(s):

Inflammatory Activity ◽

Anti Inflammatory

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IDENTIFICATION, CHARACTERIZATION OF CAROTENOID PIGMENT PRODUCING BACTERIA FROM VEGETABLE, FRUITS AND MINI-SURVEY ON PERCEPTION OF URBAN POPULATION TOWARDS BACTERIAL PIGMENTS

International Journal of Scientific and Engineering Research ◽

10.14299/ijser.2021.11.04 ◽

2021 ◽

Vol 12 (11) ◽

pp. 538-560

Author(s):

Chinmayee Mahadik ◽

Neha Manoti ◽

Aafra Zuzar Mujawar ◽

Meera Nambidas Konar ◽

Peenal Arvind Mistry ◽

...

Keyword(s):

Urban Population ◽

Great Promise ◽

Carotenoid Pigment ◽

Natural Pigment ◽

Pharmaceutical Industries ◽

Anti Inflammatory ◽

Synthetic Pigments ◽

Toxic Reactions ◽

Bacterial Pigments

Pigments are largely exploited compounds due to their chromophoric variations and enticing properties in several applications such as food, dairy, printing, textile and pharmaceutical industries etc. While synthetic pigments are widely used, toxic reactions and effects of the pigments has led to the prompt diversion of consumers towards natural pigment sources such as microbial pigments (MP). MP hold great promise against existent synthetic counterparts with additional properties such as biodegradability, non-toxicity, therapeutics (anti-inflammatory, antioxidant, anticancer etc.

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