scholarly journals Oyster-Derived Zinc-Binding Peptide Modified by Plastein Reaction via Zinc Chelation Promotes the Intestinal Absorption of Zinc

Marine Drugs ◽  
2019 ◽  
Vol 17 (6) ◽  
pp. 341 ◽  
Author(s):  
Li ◽  
Gong ◽  
Wang ◽  
Gao ◽  
Ren ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Zinc Binding ◽  
Peptide Transport ◽  
Zinc Absorption ◽  
Absorption Mechanism ◽  
Transport Pathway ◽  
Binding Peptide ◽  
Plastein Reaction ◽  
Zinc Bioavailability ◽  
Binding Peptides

Zinc-binding peptides from oyster (Crassostrea gigas) have potential effects on zinc supplementation. The aim of this study was to prepare efficient zinc-binding peptides from oyster-modified hydrolysates by adding exogenous glutamate according to the plastein reaction and to further explore the zinc absorption mechanism of the peptide-zinc complex (MZ). The optimum conditions for the plastein reaction were as follows: pH 5.0, 40 °C, substrate concentration of 40%, pepsin dosage of 500 U/g, reaction time of 3 h and l-[1-13C]glutamate concentration of 10 mg/mL. The results of 13C isotope labelling suggested that the addition of l-[1-13C]glutamate contributed to the increase in the zinc-binding capacity of the peptide. The hydrophobic interaction was the main mechanism of action of the plastein reaction. Ultraviolet spectra and scanning electronic microscopy (SEM) revealed that the zinc-binding peptide could bind with zinc and form MZ. Furthermore, MZ could significantly enhance zinc bioavailability in the presence of phytic acid, compared to the commonly used ZnSO4. Additionally, MZ significantly promoted the intestinal absorption of zinc mainly through two pathways, the zinc ion channel and the small peptide transport pathway. Our work attempted to increase the understanding of the zinc absorption mechanism of MZ and to support the potential application of MZ as a supplementary medicine.

scholarly journals New In Vitro Coculture Model for Evaluating Intestinal Absorption of Different Lipid Nanocapsules

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 595
Author(s):  
Norraseth Kaeokhamloed ◽  
Emillie Roger ◽  
Jérôme Béjaud ◽  
Nolwenn Lautram ◽  
Florence Manero ◽  
...  
Keyword(s):  
Surface Modification ◽  
Intestinal Absorption ◽  
Oral Absorption ◽  
Membrane Integrity ◽  
Microvascular Endothelial Cell ◽  
Absorption Mechanism ◽  
Apparent Permeability ◽  
Lipid Nanocapsules ◽  
Coculture Model

Standard models used for evaluating the absorption of nanoparticles like Caco-2 ignore the presence of vascular endothelium, which is a part of the intestinal multi-layered barrier structure. Therefore, a coculture between the Caco-2 epithelium and HMEC-1 (Human Microvascular Endothelial Cell type 1) on a Transwell® insert has been developed. The model has been validated for (a) membrane morphology by transmission electron microscope (TEM); (b) ZO-1 and β-catenin expression by immunoassay; (c) membrane integrity by trans-epithelial electrical resistance (TEER) measurement; and (d) apparent permeability of drugs from different biopharmaceutical classification system (BCS) classes. Lipid nanocapsules (LNCs) were formulated with different sizes (55 and 85 nm) and surface modifications (DSPE-mPEG (2000) and stearylamine). Nanocapsule integrity and particle concentration were monitored using the Förster resonance energy transfer (FRET) technique. The result showed that surface modification by DSPE-mPEG (2000) increased the absorption of 55-nm LNCs in the coculture model but not in the Caco-2. Summarily, the coculture model was validated as a tool for evaluating the intestinal absorption of drugs and nanoparticles. The new coculture model has a different LNCs absorption mechanism suggesting the importance of intestinal endothelium and reveals that the surface modification of LNCs can modify the in vitro oral absorption.

Baclofen: Intestinal absorption mechanism in mice

1996 ◽  
Vol 27 (7) ◽  
pp. 1269-1271 ◽  
Author(s):  
Graciela N. Balerio ◽  
Modesto C. Rubio
Keyword(s):  
Intestinal Absorption ◽  
Absorption Mechanism

Absorption Properties of Luteolin and Apigenin in Genkwa Flos Using In Situ Single-Pass Intestinal Perfusion System in the Rat

2017 ◽  
Vol 45 (08) ◽  
pp. 1745-1759 ◽  
Author(s):  
Xin He ◽  
Zi-Jing Song ◽  
Cui-Ping Jiang ◽  
Chun-Feng Zhang
Keyword(s):  
Small Intestine ◽  
Intestinal Absorption ◽  
Rat Model ◽  
Clinical Effectiveness ◽  
Intestinal Perfusion ◽  
Flower Bud ◽  
Transport Pathway ◽  
Absorption Enhancers ◽  
Single Pass

The flower bud of Daphne genkwa (Genkwa Flos) is a commonly used herbal medicine in Asian countries. Luteolin and apigenin are two recognized active flavonoids in Genkwa Flos. The aim of this study was to investigate the intestinal absorption mechanisms of Genkwa Flos flavonoids using in situ single-pass intestinal perfusion rat model. Using HPLC, we determined its major effective flavonoids luteolin, apigenin, as well as, hydroxygenkwanin and genkwanin in biological samples. The intestinal absorption mechanisms of the total flavonoids in Genkwa Flos (TFG) were investigated using in situ single-pass intestinal perfusion rat model. Comparing the TFG absorption rate in different intestinal segments, data showed that the small intestine absorption was significantly higher than that of the colon ([Formula: see text]). Compared with duodenum and ileum, the jejunum was the best small intestinal site for TFG absorption. The high TFG concentration (61.48[Formula: see text][Formula: see text]g/ml) yielded the highest permeability ([Formula: see text]). Subsequently, three membrane protein inhibitors (verapamil, pantoprazole and probenecid) were used to explore the TFG absorption pathways. Data showed probenecid, a multidrug resistance protein (or MRP) inhibitor, effectively enhanced the TFG absorption ([Formula: see text]). Furthermore, by comparing commonly used natural absorption enhancers on TFG, it was observed that camphor was the most effective. In Situ single-pass intestinal perfusion experiment shows that TFG absorption is much higher in the small intestine than in the colon, and the TFG is absorbed mainly via an active transport pathway with MRP-mediated efflux mechanism. Camphor obviously enhanced the TFG absorption, and this could be an effective TFG formulation preparation method to increase clinical effectiveness after Genkwa Flos administration. Our study elucidated the TFG absorption mechanisms, and provided new information for its formulation preparation.

Surface functionalization of polymer substrates with hydroxyapatite using polymer-binding peptides

2016 ◽  
Vol 4 (21) ◽  
pp. 3651-3659 ◽  
Author(s):  
Kazutoshi Iijima ◽  
Hiroumi Nagahama ◽  
Akari Takada ◽  
Toshiki Sawada ◽  
Takeshi Serizawa ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Body Fluids ◽  
Binding Peptide ◽  
Polymer Substrates ◽  
Binding Peptides ◽  
Simulated Body Fluids ◽  
Functional Peptides

Polymer substrates were modified with hydroxyapatite (HAp) using two bi-functional peptides consists of polymer-binding peptide and triasparate for HAp mineralization in simulated body fluids and HAp-binding peptide for immobilization HAp nanoparticles.

Lipid Absorption and Deposition in Rainbow Trout (Salmo gairdnerii)

1973 ◽  
Vol 51 (7) ◽  
pp. 1050-1058 ◽  
Author(s):  
J. Stephen Robinson ◽  
James F. Mead
Keyword(s):  
Fatty Acids ◽  
Rainbow Trout ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Intestinal Absorption ◽  
Lipid Absorption ◽  
Portal System ◽  
Absorption Mechanism ◽  
Dark Muscle ◽  
Muscle Lipids

Rainbow trout, fed or starved for 5 weeks, were force-fed 23 μCi 1-14C-palmitic acid and sacrificed at [Formula: see text], 1, 2, 4, 8, and 14 h after feeding. Analysis of the blood suggested that trout possess an intestinal absorption mechanism for lipid that is very different from that found in other animals. The fish, incapable of delivering triglyceride to its circulation in the usual way, receives most of its absorbed lipid as free fatty acids, probably via the portal system. It was also found that light muscle more easily yielded its triglyceride stores, in agreement with its role as the major lipid depot in trout. Nonetheless, dark muscle was metabolically more active in deposition of muscle lipids. Dark muscle appeared to function more as an organ than a kinetically responsible contractile body.

Organic crystal-binding peptides: morphology control and one-pot formation of protein-displaying organic crystals

Nanoscale ◽  
2015 ◽  
Vol 7 (47) ◽  
pp. 20155-20163 ◽  
Author(s):  
Teppei Niide ◽  
Kyohei Ozawa ◽  
Hikaru Nakazawa ◽  
Daniel Oliveira ◽  
Hitoshi Kasai ◽  
...  
Keyword(s):  
Crystal Morphology ◽  
Protein Immobilization ◽  
Morphology Control ◽  
Organic Crystal ◽  
Organic Crystals ◽  
One Pot ◽  
Binding Peptide ◽  
Simultaneous Control ◽  
Crystal Binding ◽  
Binding Peptides

We generated perylene crystal-binding peptide, which can be used for simultaneous control of perylene crystal morphology, dispersion, and protein immobilization on the crystals.

Intestinal absorption of zinc: a role for a zinc-binding ligand in milk.

1978 ◽  
Vol 235 (5) ◽  
pp. E556
Author(s):  
J R Duncan ◽  
L S Hurley
Keyword(s):  
Human Milk ◽  
Intestinal Mucosa ◽  
Neonatal Period ◽  
Bovine Milk ◽  
Zinc Binding ◽  
Acrodermatitis Enteropathica ◽  
Zinc Absorption ◽  
Human Infants ◽  
Normal Human ◽  
Old Rats

This study examined the proposal that a low molecular weight, zinc-binding ligand (ZBL) in certain milks is important for zinc absorption in the neonatal period. Ten-day-old rats, in which intestinal ZBL is absent, fed (by stomach intubation) 65Zn-labeled ZBL from rat milk, absorbed significantly more 65Zn than those fed free 65ZnCl2 or bovine milk fractions. ZBL from human milk appeared to have an intermediate effect, possibly due to species specificity. 65Zn was found in the ZBL fraction in intestinal mucosa of 10-day-old rats fed rat or human milk fractions, but not in those fed bovine milk or free 65ZnCl2. In contrast, in 18-day-old rats, which have an endogenous intestinal ZBL, there were no differences in zinc absorption, and any of the labeled milk fractions or free 65Zn caused localization of 65Zn in the ZBL fraction of intestinal mucosa. These results support the hypothesis that the intestinal ZBL plays a role in zinc absorption and that in the neonatal period before its development the milk ZBL is valuable for this function. This mechanism may be important in normal human infants as well as in acrodermatitis enteropathica patients.

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