scholarly journals Effect of Temperature and Flow Rate on the Cell-Free Area in the Microfluidic Channel

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 109
Author(s):  
Angeles Ivón Rodríguez-Villarreal ◽  
Manuel Carmona-Flores ◽  
Jordi Colomer-Farrarons
Keyword(s):  
Blood Cell ◽  
Flow Rate ◽  
Red Blood Cell ◽  
Microfluidic Channel ◽  
Cell Manipulation ◽  
Effect Of Temperature ◽  
Autologous Plasma ◽  
Interesting Task ◽  
Flow Rate Increase ◽  
Free Area

Blood cell manipulation in microdevices is an interesting task for the separation of particles, by their size, density, or to remove them from the buffer, in which they are suspended, for further analysis, and more. This study highlights the cell-free area (CFA) widening based on experimental results of red blood cell (RBC) flow, suspended in a microfluidic device, while temperature and flow rate incrementally modify RBC response within the microflow. Studies of human red blood cell flow, at a concentration of 20%, suspended in its autologous plasma and phosphate-buffered saline (PBS) buffer, were carried out at a wide flow rate, varying between 10 and 230 μL/min and a temperature range of 23 °C to 50 °C. The plotted measures show an increment in a CFA near the channel wall due to cell flow inertia after a constricted channel, which becomes more significant as temperature and flow rate increase. The temperature increment widened the CFA up to three times. In comparison, flow rate increment increased the CFA up to 20 times in PBS and 11 times in plasma.

scholarly journals Probing red blood cell mechanics, rheology and dynamics with a two-component multi-scale model

2014 ◽  
Vol 372 (2021) ◽  
pp. 20130389 ◽  
Author(s):  
Xuejin Li ◽  
Zhangli Peng ◽  
Huan Lei ◽  
Ming Dao ◽  
George Em Karniadakis
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Microfluidic Channel ◽  
Scale Model ◽  
Cross Sectional ◽  
Whole Cell ◽  
Multi Scale ◽  
Rbc Membrane ◽  
Two Component

This study is partially motivated by the validation of a new two-component multi-scale cell model we developed recently that treats the lipid bilayer and the cytoskeleton as two distinct components. Here, the whole cell model is validated and compared against several available experiments that examine red blood cell (RBC) mechanics, rheology and dynamics. First, we investigated RBC deformability in a microfluidic channel with a very small cross-sectional area and quantified the mechanical properties of the RBC membrane. Second, we simulated twisting torque cytometry and compared predicted rheological properties of the RBC membrane with experimental measurements. Finally, we modelled the tank-treading (TT) motion of a RBC in a shear flow and explored the effect of channel width variation on the TT frequency. We also investigated the effects of bilayer–cytoskeletal interactions on these experiments and our simulations clearly indicated that they play key roles in the determination of cell membrane mechanical, rheological and dynamical properties. These simulations serve as validation tests and moreover reveal the capabilities and limitations of the new whole cell model.

Examination of the relation between red blood cell aggregation and hematocrit in human and various experimental animals

2021 ◽  
pp. 1-12
Author(s):  
Barbara Barath ◽  
Viktoria Somogyi ◽  
Bence Tanczos ◽  
Adam Varga ◽  
Zsuzsanna Bereczky ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Species Differences ◽  
Light Transmission ◽  
Hematological Parameters ◽  
Light Reflection ◽  
Reflection Method ◽  
Transmission Method ◽  
Autologous Plasma ◽  
Rbc Aggregation

BACKGROUND: Red blood cell (RBC) aggregation plays an important role in the physiological processes of the microcirculation. The complete mechanism of aggregation is still unclear, and it is influenced by several cellular and plasmatic factors. One of these factors is the hematocrit (Hct). OBJECTIVE: We hypothesized that the relation of RBC aggregation and Hct differs between species. METHODS: From anticoagulated blood samples of healthy volunteers, rats, dogs, and pigs, 20, 40, and 60 %Hct RBC, autologous plasma suspensions were prepared. Hematological parameters and RBC aggregation was determined by light-transmission and light-reflection method. RESULTS: Suspensions at 20%and 60%Hct expressed lower RBC aggregation than of 40%Hct suspensions, showing inter-species differences. By curve fitting the Hct at the highest aggregation value differed in species (human: 45.25%- M 5 s, 40.86%- amp;rat: 44.44 %- M1 10 s, 39.37%- amp; dog: 42.48%- M 5 s, 44.29%- amp; pig: 47.63%- M 5 s, 52.8%- amp). CONCLUSION: RBC aggregation - hematocrit relation shows inter-species differences. Human blood was found to be the most sensitive for hematocrit changes. The more obvious differences could be detected by M 5 s by light-transmission method and amplitude parameter using light-reflection method.

Nitric oxide generation by endothelial cells exposed to shear stress in glass tubes perfused with red blood cell suspensions: role of aggregation

2008 ◽  
Vol 294 (5) ◽  
pp. H2098-H2105 ◽  
Author(s):  
Ozlem Yalcin ◽  
Pinar Ulker ◽  
Ugur Yavuzer ◽  
Herbert J. Meiselman ◽  
Oguz K. Baskurt
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Vessel Wall ◽  
Plasma Viscosity ◽  
Autologous Plasma ◽  
Rbc Aggregation

Endothelial function is modulated by wall shear stress acting on the vessel wall, which is determined by fluid velocity and the local viscosity near the vessel wall. Red blood cell (RBC) aggregation may affect the local viscosity by favoring axial migration. The aim of this study was to investigate the role of RBC aggregation, with or without altered plasma viscosity, in the mechanically induced nitric oxide (NO)-related mechanisms of endothelial cells. Human umbilical vein endothelial cells (HUVEC) were cultured on the inner surface of cylindrical glass capillaries that were perfused with RBC suspensions having normal and increased aggregation at a nominal shear stress of 15 dyn/cm2. RBC aggregation was enhanced by two different approaches: 1) poloxamer-coated RBC suspended in normal, autologous plasma, resulting in enhanced aggregation but unchanged plasma viscosity and 2) normal RBC suspended in autologous plasma containing 0.5% dextran (mol mass 500 kDa), with a similar level of RBC aggregation but higher plasma viscosity. Compared with normal cells in unmodified plasma, perfusion with suspensions of poloxamer-coated RBC in normal plasma resulted in decreased levels of NO metabolites and serine 1177 phosphorylation of endothelial nitric oxide synthase (eNOS). Perfusion with normal RBC in plasma containing dextran resulted in a NO level that remained elevated, whereas only a modest decrease of phosphorylated eNOS level was observed. The results of this study suggest that increases of RBC aggregation tendency affect endothelial cell functions by altering local blood composition, especially if the alterations of RBC aggregation are due to modified cellular properties and not to plasma composition changes.

scholarly journals Quantitative absorption imaging of red blood cells to determine physical and mechanical properties

RSC Advances ◽  
2020 ◽  
Vol 10 (64) ◽  
pp. 38923-38936
Author(s):  
Ratul Paul ◽  
Yuyuan Zhou ◽  
Mehdi Nikfar ◽  
Meghdad Razizadeh ◽  
Yaling Liu
Keyword(s):  
Mechanical Properties ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Microfluidic Channel ◽  
Physical And Mechanical Properties ◽  
Constant Thickness ◽  
Absorption Imaging ◽  
Quantitative Absorption

The constant thickness in the microfluidic channel is used for controlled absorption of red and blue light to measure red blood cell hemoglobin and height mapping. High speed recording of the height mapping provides us the membrane fluctuation.

Optical detection of red blood cell aggregation in a disposable microfluidic channel

2005 ◽  
Vol 19 (3) ◽  
pp. 887-893
Author(s):  
Sehyun Shin ◽  
Ju Hee Jang ◽  
Myung Soo Park ◽  
Yunhee Ku ◽  
Jang-Soo Suh
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Optical Detection ◽  
Microfluidic Channel ◽  
Cell Aggregation ◽  
Red Blood Cell Aggregation ◽  
Blood Cell Aggregation
Sign in / Sign up

Export Citation Format

Share Document